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Multiplexed stimulated emission depletion nanoscopy(mSTED)for 5-color live-cell long-term imaging of organelle interactome

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Opto-Electronic Advances 2024年第7期7卷 17-26页

作者： Yuran Huang zhimin Zhang Wenli tao Yunfei Wei Liang Xu Wenwen Gong Jiaqiang Zhou Liangcai Cao Yong Liu Yubing Han Cuifang Kuang Xu Liu State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and EngineeringZhejiang UniversityHangzhou 310027China Research Center for Intelligent Chips and Devices Zhejiang LabHangzhou 311121China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China Department of Endocrinology and Metabolism Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Precision Instruments Tsinghua UniversityBeijing 100084China College of electronics and information engineering Shanghai University of Electrical PowerShanghai 200090China ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 311200China

Stimulated emission depletion microscopy(STED)holds great potential in biological science applications,especially in studying nanoscale subcellular ***,multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser ***,we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and *** separating live-cell fluorescent probes with similar spectral properties using phasor analysis,our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular *** results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell.

关键词： optical nanoscopy phasor analysis multicolor live cell imaging

Effect of arotinolol on right ventricular function in patients with dilated cardiomyopathy

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Journal of Geriatric Cardiology 2007年第3期4卷 170-173页

作者： Hong Yang Li Xu Yongkang tao zhimin Xu Xiuqing Du Naqiang Lu Kefei Dou Jinglin Zhao Xianqi Yuan Yanfen Zhao Rongfang Shi Chaomei Fan Key Laboratory of Clinical Trials Research in Cardiovascular Drugs Ministry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Beijing Nuclear Industry Hospital Beijing 100042China. Beijing Nuclear Industry Hospital Beijing 100042China. Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037China Key Laboratory of Clinical Trials Research in Cardiovascular DrugsMinistry of HealthClinical Pharmacology CenterCardiovascular Institute and Fuwai Hospital CAMS and PUMCBeijing 100037Ch

Objective Dilated cardiomyopathy(DCM) is generally considered to be accompanied by both left and right ventricular dysfunction,but most studies only analyze the left ventricular function. In this study,we evaluated the effect of arotinolol on right ventricular function in patients with DCM. Methods Right ventricular ejection fraction(RVEF) and right ventricular diameter(RVD) were measured by two-dimensional echocardiography(2-DE) in 33 DCM patients;RVEF measured by first-pass radionuclide angiography(FPRA) was compared with that by 2-DE. Results The treatment with arotinolol for one year resulted in a reduction in the right ventricular diameter(baseline,23.0 ± 8.3 mm vs after one-year treatment,20.7 ± 5.4 mm;P=0.004 ) and an associated increase in ejection fraction(baseline,36.9 ± 10.3% vs after one-year treatment,45.8 ± 9.6%;P < 0.001 ) ;there is a high correlation between the 2-DE method and radionuclide ventriculographic method. The correlation coefficient is 0.933(P<0.001) . Conclusion Arotinolol therapy could not only improve left ventricular function,but also improve right ventricular function in DCM patients.

关键词： dilated cardiomyopathy arotinolol right ventricular function

Genome-wide CRISPR screening identifies critical role of phosphatase and tensin homologous (PTEN) in sensitivity of acute myeloid leukemia to chemotherapy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第8期25卷 700-710页

作者： Liming LIN Jingjing tao Ying MENG Yichao GAN Xin HE Shu LI Jiawei ZHANG Feiqiong GAO Dijia XIN Luyao WANG Yili FAN Boxiao CHEN zhimin LU Yang XU Department of Hematology the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Zhejiang Provincial Key Laboratory of Pancreatic Disease the First Affiliated Hospital and Institute of Translational MedicineCancer CenterZhejiang University School of MedicineHangzhou 310029China Institute of Genetics Zhejiang UniversityHangzhou 310058China Division of Hematopoietic Stem Cell&Leukemia Research Beckman Research Institute of City of HopeDuarteCA 91010USA Department of Hematology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

Although significant progress has been made in the development of novel targeted drugs for the treatment of acute myeloid leukemia(AML)in recent years,chemotherapy still remains the mainstay of treatment and the overall survival is poor in most ***,we demonstrated the antileukemia activity of a novel small molecular compound NL101,which is formed through the modification on bendamustine with a suberanilohydroxamic acid(SAHA)***101 suppresses the proliferation of myeloid malignancy cells and primary AML *** induces DNA damage and caspase 3-mediated apoptosis.A genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)library screen revealed that phosphatase and tensin homologous(PTEN)gene is critical for the regulation of cell survival upon NL101 *** knockout or inhibition of PTEN significantly reduced NL101-induced apoptosis in AML and myelodysplastic syndrome(MDS)cells,accompanied by the activation of protein kinase B(AKT)signaling *** inhibition of mammalian target of rapamycin(mTOR)by rapamycin enhanced the sensitivity of AML cells to NL101-induced cell *** findings uncover PTEN protein expression as a major determinant of chemosensitivity to NL101 and provide a novel strategy to treat AML with the combination of NL101 and rapamycin.

关键词： Genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)library Phosphatase and tensin homologous(PTEN) Rapamycin Acute myeloid leukemia(AML) Chemotherapy

Shared genetic architecture highlights the bidirectional association between major depressive disorder and fracture risk

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General Psychiatry 2024年第3期37卷 352-363页

作者： Pianpian Zhao zhimin Ying Chengda Yuan Haisheng Zhang Ao Dong Jianguo tao Xiangjiao Yi Mengyuan Yang Wen Jin Weiliang Tian David Karasik Geng Tian Houfeng Zheng The Affiliated Hangzhou First People’s Hospital School of MedicineWestlake UniversityHangzhouZhejiangChina Westlake Laboratory of Life Sciences and Biomedicine HangzhouZhejiangChina Diseases&Population(DaP)Geninfo Lab School of Life SciencesWestlake UniversityHangzhouZhejiangChina Institute of Basic Medical Sciences Westlake Institute for Advanced StudyHangzhouZhejiangChina Department of Orthopedic Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina Department of Dermatology Hangzhou Hospital of Traditional Chinese MedicineHangzhouZhejiangChina Department of Global Statistics Eli Lilly and CompanyBranchburgNew JerseyUSA Azrieli Faculty of Medicine Bar-Ilan UniversitySafedIsrael Binzhou Medical University YantaiShandongChina

Background There is limited evidence suggesting that osteoporosis might exacerbate depressive symptoms,while more studies demonstrate that depression negatively affects bone density and increases fracture *** To explore the relationship between major depressive disorder(MDD)and fracture *** We conducted a nested case-control analysis(32670 patients with fracture and 397017 individuals without fracture)and a matched cohort analysis(16496 patients with MDD and 435492 individuals without MDD)in the same prospective UK Biobank data ***,we investigated the shared genetic architecture between MDD and fracture with linkage disequilibrium score regression and the MiXeR statistical *** used the conditional/conjunctional false discovery rate approach to identify the specific shared *** calculated the weighted genetic risk score for individuals in the UK Biobank and logistic regression was used to confirm the association observed in the prospective *** We found that MDD was associated with a 14%increase in fracture risk(hazard ratio(HR)1.14,95%CI 1.14 to 1.15,p<0.001)in the nested case-control analysis,while fracture was associated with a 72%increase in MDD risk(HR 1.72,95%CI 1.64 to 1.79,p<0.001)in the matched cohort analysis,suggesting a longitudinal and bidirectional ***,genetic summary data suggested a genetic overlap between MDD and ***,we identified four shared genomic loci,with the top signal(rs7554101)near *** protein encoded by SGIP1 is involved in cannabinoid receptor type 1 *** found that genetically predicted MDD was associated with a higher risk of fracture and vice *** addition,we found that the higher expression level of SGIP1 in the spinal cord and muscle was associated with an increased risk of fracture and *** The genetic pleiotropy between MDD and fracture highlights the bidirectional association observed in the epidemiological *** shared ge

关键词： fracture matched osteoporosis

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基于等效FE-BEM法的层合板隔声性能

航空动力学报 2024年第2期39卷 9-16页

作者：彭涛侯峰邹学锋戚志民韩啸中国飞机强度研究所航空声学与振动航空科技重点实验室西安710065 中国飞机强度研究所强度与结构完整性全国重点实验室西安710065 中国飞机强度研究所陕西省飞行器振动冲击与噪声重点实验室西安710065

以对称层合板结构为对象,基于经典层合板理论,将其等效为单层各项异性板,采用FE-BEM法(hybrid finite element-boundary element method)分析了其在宽频噪声激励作用下的隔声特性。为验证等效方法的适用性,开展对称复合材料层合板模态... 详细信息

以对称层合板结构为对象,基于经典层合板理论,将其等效为单层各项异性板,采用FE-BEM法(hybrid finite element-boundary element method)分析了其在宽频噪声激励作用下的隔声特性。为验证等效方法的适用性,开展对称复合材料层合板模态测试和数值分析;为验证预估结论的正确性,将FE-BEM法结果与FE-SEA法(hybrid finite element-statistic energy analysis)结果、试验结果分别进行对比。结果表明:将等效方法用于对称层合板固有特性模拟是正确的,等效层合板的固有特性的仿真结果与试验值一致,误差在6.9%以内;等效方法和FE-BEM法结合进行对称层合板隔声预计是有效的,FE-BEM法预计结果与试验结果吻合良好;等效方法和FE-SEA法结合进行对称层合板隔声预计也是有效的,FE-BEM法比FE-SEA法所建模型计算耗时长,算例中计算耗时增大4.4%。

关键词： FE-BEM 隔声特性复合材料宽频激励层合板

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护理本科生皮肤损伤性职业暴露现状调查

中国实用护理杂志 2015年第17期31卷 1254-1257页

作者：陶志敏杨长永河南大学护理学院开封475004

目的了解护理本科生皮肤损伤性职业暴露的现状并分析其原因，为制订科学的护理本科实习生职业防护教育措施提供参考依据。方法自行设计调查表，采取方便抽样的方法，抽取河南省2所本科院校的护理毕业生260人为调查对象，对影响皮肤损伤... 详细信息

目的了解护理本科生皮肤损伤性职业暴露的现状并分析其原因，为制订科学的护理本科实习生职业防护教育措施提供参考依据。方法自行设计调查表，采取方便抽样的方法，抽取河南省2所本科院校的护理毕业生260人为调查对象，对影响皮肤损伤的相关原因进行分析。结果最终有效问卷238份。本科实习护生皮肤损伤的发生率为96．64％（230／238），实习初期发生率较高；导致皮肤损伤的物品主要是空心针头和玻璃安瓿；皮肤损伤时进行的操作环节主要集中在配药和回套针帽；皮肤损伤后进行的操作一般为挤出血液、流水冲洗和碘酊加乙醇的消毒，查看患者检验单的仅有58．47％（473／809）；缺乏上报意识。结论护理本科生在实习中皮肤损伤发生率较高，主要原因是操作不规范，防护意识淡漠；皮肤损伤后多为一般性处理，对损伤后的危害认识不足，需要加强护生岗前职业防护的规范化教育、健全职业暴露后处理及报告制度。

关键词：护理职业暴露问卷调查皮肤损伤

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急性心肌梗死病人康复期家庭护理模式的探讨

护理研究（上旬版） 2007年第8期21卷 2060-2062页

作者：靳艳陶志敏河南大学护理学院河南大学第一附属医院

针对急性心肌梗死病人及家属的需求,通过对病人疾病及家庭资料的评估,制订相应个体化的护理计划和目标,落实护理措施,给予效果评价。旨在探求急性心肌梗死康复期家庭护理模式,促进病人康复,使病人和家属均受益,使护理质量得到提高。

关键词：急性心肌梗死家庭护理康复

原发性高血压患者大动脉弹性功能及其与年龄关系分析

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中国现代医生 2007年第8Z期45卷 25-26页

作者：靳艳陶志敏河南大学第一附属医院河南大学护理学院河南开封475001

目的探讨原发性高血压患者大动脉弹性功能的改变及其与年龄的关系。方法应用脉搏波速度自动测量系统对58例健康人和239例高血压患者进行颈动脉-股动脉脉搏波速度检测。结果原发性高血压患者的CFPWV显著高于健康人(P<0.01)。年龄是影响... 详细信息

目的探讨原发性高血压患者大动脉弹性功能的改变及其与年龄的关系。方法应用脉搏波速度自动测量系统对58例健康人和239例高血压患者进行颈动脉-股动脉脉搏波速度检测。结果原发性高血压患者的CFPWV显著高于健康人(P<0.01)。年龄是影响健康人大动脉弹性功能最主要的因素。结论增龄导致大动脉弹性功能减退,高血压加速这一过程;脉搏波速度检测为临床提供一种无创、简便的评估高血压患者血管功能状态的方法。

关键词：大动脉弹性脉搏波速度高血压

离心式高速玉米精量排种器T形槽型孔设计与试验

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农业工程学报 2024年第7期40卷 50-60页

作者：杨丽李治民张东兴李川崔涛和贤桃中国农业大学工学院北京100083 农业农村部土壤-机器-植物系统技术重点实验室北京100083

针对离心式高速玉米精量排种器双粒种子并排填充导致重播严重的问题,该研究提出利用型孔槽对称凸台结构降低玉米种子并排填充概率的方法,设计了一种T形槽型孔。通过构建充种阶段玉米种子的力学模型,并结合玉米种子的形状特征确定了T形... 详细信息

针对离心式高速玉米精量排种器双粒种子并排填充导致重播严重的问题,该研究提出利用型孔槽对称凸台结构降低玉米种子并排填充概率的方法,设计了一种T形槽型孔。通过构建充种阶段玉米种子的力学模型,并结合玉米种子的形状特征确定了T形槽型孔基本结构参数。借助EDEM离散元仿真软件,以型孔槽前端长度、型孔槽后端面倾斜角以及型孔槽底部倾斜角为因素,以合格指数、重播指数与漏播指数为评价指标,设计二次正交旋转回归组合仿真试验。仿真试验结果表明:在作业速度18 km/h条件下,型孔槽前端长度为9.31 mm,型孔槽后端面倾斜角为43.37°与型孔槽底部倾斜角为70.5°时,排种质量最优,排种合格指数、重播指数与漏播指数分别为94.03%、1.72%与4.25%。台架验证试验结果表明:作业速度为15 km/h时,T形槽型孔的合格指数最高为95.16%,在此作业速度下,排种器的重播指数为1.42%,漏播指数为3.42%,满足高速精量播种要求;作业速度在12~21 km/h内时,T形槽型孔的平均合格指数为94.77%,相较于矩形槽型孔提升了3.13个百分点,相较于蹄型槽型孔提升了1.80个百分点,T形槽型孔的平均重播指数为2.06%,相较于矩形槽型孔降低了3.41个百分点,相较于蹄型槽型孔降低了2.49个百分点。该研究可为离心式高速玉米精量排种器优化提供参考。

关键词：农业机械仿真玉米高速播种型孔 EDEM