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Oil Crop Science 2021年第2期6卷 58-65页

作者： Hongli Yang Wei Guo Dong Cao Haifeng Chen Shuilian Chen Zhonglu Yang zhihui shan Limiao Chen Xinan Zhou Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan430062China

Auxin has been reported to regulate plant growth and development,as well as to mediate plant adaption to abiotic stresses,including ***/LAX family displays auxin uptake functions and comprises four highly conserved genes AUX1 and LIKE AUX1(LAX1,LAX2,and LAX3)in *** are fifteen GmLAX family genes in the soybean genomes and several members were regulated by dehydration *** this study,the sequence differences and expression pattern of GmLAXs-I were analyzed under stress treatment between the soybean droughttolerant Jindou 21 and drought-sensitive varieties Zhongdou *** homologous genes of AUX1 were all responsive to PEG,salt,ABA and IAA *** were two SNPs in the promoter region of GmLAX4 gene,and this gene was differentially expressed in two ***,our results showed YFP-GmLAXs are predominantly localized in plasma *** together,our results suggest that GmLAXs are involved in abiotic response,which can provide theoretical and technical support for the genetic improvement of soybean drought tolerance.

关键词： Glycine max GmLAXs Expression profile Subcellular localization

A natural allelic variant of GmSW17.1 confers high 100-seed weight in soybean

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The Crop Journal 2024年第6期12卷 1709-1717页

作者： Hao Zhang Lei Yang Shiyu Guo Yu Tian Chunyan Yang Chaosen Zhao zhihui shan Qi Wang Zihao Zheng Ying-hui Li Li-juan Qiu State Key Laboratory of Crop Gene Resources and Breeding/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Institute of Cereal and Oil Crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang Branch Center of National Center for Soybean ImprovementThe Key Laboratory of Crop Genetics and BreedingShijiazhuang 050031HebeiChina Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Nanchang Branch of National Centre of Oil Crops Improvement/Jiangxi Province Key Laboratory of Oil Crops Biology Nanchang 330200JiangxiChina Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of AgricultureWuhan 430062HubeiChina Department of Agronomy Iowa State UniversityAmesIA 50011-1051USA

Soybean(Glycine max)is an important and valuable crop,providing oil and proteins for both humans and *** weight is a key trait that determines soybean yields;however,the genes and mechanisms controlling seed weight remain poorly ***,we used genome-wide association study(GWAS)and joint linkage mapping to identify a ubiquitin-specific protease,GmSW17.1,which regulates 100-seed weight in *** natural allelic variants of GmSW17.1 resulted in significantly different 100-seed weight,with GmSW17.1T conferring heavier *** used CRISPR/Cas9 technology to knock out GmSW17.1,resulting in lighter and smaller seeds;however,these mutants produced more seeds than the wild type,resulting in similar overall *** to the increased number of seeds,we determined that GmSW17.1 is highly transcribed in developing seeds,and its encoded protein physically interacts in the nucleus with GmSGF11,which plays a crucial role in the deubiquitinating *** of genomic sequences from more than 1714 soybean accessions suggested that the natural allele GmSW17.1T was selected during the domestication and genetic improvement,resulting in its rapid expansion in cultivated *** findings provide important insights into the role of GmSW17.1 in 100-seed weight and offer valuable clues for the molecular breeding of soybean.

关键词： GWAS GmSW17.1 Natural allelic variant 100-Seed weight Soybean

Herbivore defense responses and associated herbivore defense mechanism as revealed by comparing a resistant wild soybean with a susceptible cultivar

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The Crop Journal 2015年第6期3卷 451-467页

作者： Xiaoyi Wang Haifeng Chen zhihui shan Qingnan Hao Chanjuan Zhang Zhonglu Yang Xiaojuan Zhang Songli Yuan Dezhen Qiu Shuilian Chen Yongqin Jiao Xin'an Zhou Key Laboratory of Oil Crop Biology of the Ministry of Agriculture Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Graduate School of the Chinese Academy of Agriculture Sciences

Plants have evolved sophisticated defense mechanisms against herbivores to help them adapt to the environment. Understanding the defense mechanisms in plants can help us control insects in a more effective manner. In this study, we found that compared with Tianlong 2(a cultivated soybean with insect susceptibility), ED059(a wild soybean line with insect resistance)contains sharper pubescence tips, as well as lower transcript levels of wound-induced protein kinase(WIPK) and salicylic acid-induced protein kinase(SIPK), which are important mitogen-activated protein kinases involved in early defense response to herbivores. The observed lower transcript levels of WIPK and SIPK induced higher levels of jasmonic acid(JA), JA biosynthesis enzymes(AOC3) and some secondary metabolites in ED059. Functional analysis of the KTI1 gene via Agrobacterium-mediated transformation in Arabidopsis thaliana indicated that it plays an important role in herbivore defense in ED059. We further investigated the molecular response of third-instar Helicoverpa armigera(Hübner) larvae to Tianlong 2 and ED059. We found apoptotic cells only in the midguts of larvae that fed on ED059. Compared with larvae reared on the susceptible cultivar Tianlong 2, transcript levels of catalase(CAT) and glutathione S-transferase(GST) were up-regulated, whereas those of CAR, CHSB, and TRY were down-regulated in larvae that fed on the highly resistant variety ED059. We propose that these differences underlie the different herbivore defense responses of ED059 and Tianlong 2.

关键词： Soybean H.armigera Morphology Gene expression level polymorphism(ELP) Detoxification and toxin-tolerance gene

Screening and identification of salt tolerance soybean varieties and germplasms

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Oil Crop Science 2024年第3期9卷 204-210页

作者： Limiao Chen Lihua Peng Wenqi Ouyang Haowen Yao Yuxin Ye zhihui shan Dong Cao Shuilian Chen Zhonglu Yang Yi Huang Bei Han Aihua Sha Xinan Zhou Haifeng Chen Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan430062China College of Agriculture Yangtze UniversityJingzhou434000China

Soil salinization is a globally prevalent abiotic environmental *** imbalance of ions caused by high concentrations of sodium chloride results in a 40%reduction in soybean ***,as an important crop for soil quality improvement,necessitates the identification of salt-tolerant varieties and germplasms to effectively utilize and enhance saline-alkali *** this study,we assessed the salt tolerance of 435 soybean varieties and germplasms during the seedling *** them,Qihuang34,You2104,Hongzhudou,Pamanheidou,and Osage exhibited grade 1 salt tolerance rates surpassing other tested ***,Hongzhudou and Qihuang34 demonstrated higher salt tolerance during germination and emergence stages based on their elevated rates of emergence,salt tolerance index,chlorophyll content,and shoot fresh *** findings provide valuable resources for molecular breeding efforts aimed at developing salt-tolerant soybean varieties suitable for cultivation in saline-alkali soils.

关键词： Soybean Varieties Germplasms Salt tolerance Seedling stage Germination and emergence stage

High efficient broad-spectrum Bradyrhizobium elkanii Y63-1

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Oil Crop Science 2023年第4期8卷 228-235页

作者： Piao Leng Fuxiao Jin Song Li Yi Huang Chanjuan Zhang zhihui shan Zhonglu Yang Limiao Chen Dong Cao Qingnan Hao Wei Guo Hongli Yang Shuilian Chen Xinan Zhou Songli Yuan Haifeng Chen Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsWuhan430062China

Soybean(Glycine max),the primary source of high-quality plant protein,plays a crucial role as a grain and oil crop in *** the full potential of symbiotic nitrogen fixation in soybean production holds immense significance for agriculture and ecology *** 63,a newly developed early-maturing summer soybean cultivar in 2021,exhibits remarkable traits such as high yield,superior quality,multi-resistance,and wide *** this study,eight distinct rhizobia strains from diverse regions were meticulously screened to identify highly effective strains specifically suited for Zhongdou *** aboveground biomass,plant height,chlorophyll content,root length,nodule number,and nodule dry weight of Zhongdou 63 were measured and the data were subjected to statistical *** results demonstrated that Y63-1 is a predominant strain of Zhongdou ***,we conducted further investigations on the broad-spectrum nodulation characteristics of *** representative soybean cultivars were individually inoculated with Y63-1 and subsequently analyzed for nodule numbers and nodule dry weight in their symbiotic systems with *** findings revealed that Y63-1 effectively formed nodules with all ten soybean varieties *** summary,our current study identified highly efficient broad-spectrum Bradyrhizobium elkanii strain Y63-1 as the predominant strain in Zhongdou 63 and provided a theoretical foundation for enhancing yield potential not only in Zhongdou 63 but also in other varieties through inoculation with highly efficient rhizobia in production.

关键词： Soybean Rhizobium Nodule symbiosis Nodulation Broad spectrum

Transcript profile of the response of two soybean genotypes to potassium deficiency

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Transcript profile of the response of two soybean genotypes ...

第23届全国大豆科研生产研讨会

作者： Cheng Wang,HaiFeng Chen,QingNan Hao,zhihui shan,Rong Zhou,HaiJian Zhi XinAn Zhou

Potassium(K)is an essential macronutrient for plant growth and *** yield potential is often affected by lack of soluble *** molecular regulation mechanism of physiological and biochemical responses to K starvation in ...

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中国大豆锈病研究(英文)

大豆科学 2006年第4期25卷 438-444页

作者：单志慧周新安中国农业科学院油料作物研究所武昌430062

1899年在中国吉林首次报道了由豆薯层锈菌(Phakopsora pachyrhiziSyd)引起的大豆锈病。20世纪60年代大豆锈病成为热带、亚热带地区大豆生产中最严重的病害。中国于20世纪70年代开始了较为系统的研究,本篇综述介绍了中国在大豆锈病的病... 详细信息

1899年在中国吉林首次报道了由豆薯层锈菌(Phakopsora pachyrhiziSyd)引起的大豆锈病。20世纪60年代大豆锈病成为热带、亚热带地区大豆生产中最严重的病害。中国于20世纪70年代开始了较为系统的研究,本篇综述介绍了中国在大豆锈病的病原菌分布及其寄主、产量损失,病害流行、病原生活周期、病原与寄主的互作、抗锈资源鉴定、抗锈遗传、抗锈育种和综合防治等方面的研究进展。

关键词：大豆锈病流行抗性机制抗锈资源生理小种

Evaluation of seed vigor in soybean germplasms from different eco-regions

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Oil Crop Science 2020年第1期5卷 22-25页

作者： Qingnan Hao Yanyan Yang Changxun Guo Xuefei Liu Haifeng Chen Zhonglu Yang Chanjuan Zhang Limiao Chen Songli Yuan Shuilian Chen Dong Cao Wei Guo Dezhen Qiu Xiaojuan Zhang zhihui shan Xin’an Zhou Oil Crops Research Institute Chinese Academy of Agricultural Scienceswuhanchina Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of AgricultureOil Crops Research Institute Chinese Academy of Agricultural Scienceswuhanchina

Seed vigor plays an essential role in soybean *** seed is sensitive to environment and easy to get seed *** soybean seed vigor can be maintained for less than one year and have to be multiplied every *** with good seed vigor are essential for maintaining planting population and stable *** this research,419 germplasms from three eco-regions,South China,Huanghuai region and Northeast China,were evaluated for seed vigor by assessing germination ratio,germination potential and germination *** 34.8%of tested germplasm had germination ratio equal or more than 85%,suggested by the national soybean seed quality standard of *** 9 from 82 tested cultivars met the seed *** germination ratio distribution had obvious regional *** performed better seed vigor than *** from South China had stronger seed vigor compared to those from Huanghuai and Northeast *** vigor has no significant relationship with maturity and 100-seed *** 21 germplasms with strong seed vigor,favor maturation and seed size were identified and can be used in future breeding program.

关键词： soybean seed vigor Eco-region

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动态数据上的高效用模式挖掘综述

计算机应用 2022年第1期42卷 94-108页

作者：单芝慧韩萌韩强北方民族大学计算机科学与工程学院银川750021

高效用模式挖掘(HUPM)考虑了项的购买数量及单位利润,提供了项更详细的信息,使用户能够做出更好的经济决策。针对大多数HUPM算法都应用在与不断产生数据的现实世界不符的静态数据集上的问题,近些年不断提出了动态数据上的HUPM算法。首先... 详细信息

高效用模式挖掘(HUPM)考虑了项的购买数量及单位利润,提供了项更详细的信息,使用户能够做出更好的经济决策。针对大多数HUPM算法都应用在与不断产生数据的现实世界不符的静态数据集上的问题,近些年不断提出了动态数据上的HUPM算法。首先,对增量数据、数据流、动态删除和动态修改数据上的HUPM算法以及融合高效用模式(高效用序列模式、平均高效用模式、top-k高效用模式等)挖掘算法进行了总结;然后,对使用不同类型数据的算法进行了总结,包括动态利润数据、动态序列数据等数据类型;其次,从算法使用的数据结构、剪枝策略、窗口模型、优缺点等角度对HUPM算法进行分类总结;最后,针对目前研究的不足,提出了下一步动态数据上的HUPM算法研究方向。

关键词：高效用模式增量数据数据流动态删除动态修改动态数据

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基于滑动窗口的数据流高效用模糊项集挖掘

南京师大学报（自然科学版） 2023年第1期46卷 120-129页

作者：单芝慧韩萌韩强北方民族大学计算机科学与工程学院宁夏银川750021 北方民族大学图像图形智能处理国家民委重点实验室宁夏银川750021

高效用项集挖掘可以提供有趣的结果集,但并不能提供单个项的数量,因此,本文提出了高效用模糊项集.但是,现实世界的数据是不断出现的,需要实时处理新到来的数据.为解决当前高效用模糊项集不能处理数据流的问题,又提出了模糊效用列表(fuzz... 详细信息

高效用项集挖掘可以提供有趣的结果集,但并不能提供单个项的数量,因此,本文提出了高效用模糊项集.但是,现实世界的数据是不断出现的,需要实时处理新到来的数据.为解决当前高效用模糊项集不能处理数据流的问题,又提出了模糊效用列表(fuzzy utility list,FUL)结构用于存储当前窗口中项的批次号、项在事务中的事务标识符、项的模糊效用以及项的剩余模糊效用,该结构能有效的对批次进行插入和删除操作.最后,基于FUL提出了数据流高效用模糊项集挖掘算法.对真实数据集和合成数据集进行了广泛的实验,结果证实了算法的效率及可行性.

关键词：数据流挖掘滑动窗口高效用项集挖掘模糊效用效用列表