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一种多重修饰血紫蛋白纳米氧载体的构建及体外性能检测

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中国组织工程研究 2025年第22期29卷 4740-4747页

作者：黄志华赵会民苏春元杨康广西医科大学第二附属医院急诊科广西壮族自治区南宁市530007 广西医科大学第二附属医院创伤中心广西壮族自治区南宁市530007 广西地中海贫血防治重点实验室广西壮族自治区南宁市530007 广西重点急危重症医学实验室广西壮族自治区南宁市530007

背景:血紫蛋白分子稳定性及生物相容性优于人和哺乳动物血红蛋白,经过修饰可能成为更加安全长效的红细胞代用品。目的:制备多重修饰血紫蛋白纳米微粒,进行理化性质表征及体外性能测试。方法:以切向流超滤法分离纯化方格星虫血紫蛋白,使... 详细信息

背景:血紫蛋白分子稳定性及生物相容性优于人和哺乳动物血红蛋白,经过修饰可能成为更加安全长效的红细胞代用品。目的:制备多重修饰血紫蛋白纳米微粒,进行理化性质表征及体外性能测试。方法:以切向流超滤法分离纯化方格星虫血紫蛋白,使用京尼平完成分子内交联,然后利用多巴胺完成纳米粒子封装,再用聚乙二醇完成钝化,获得多重修饰血紫蛋白纳米粒,表征该纳米粒的理化性质。将不同质量浓度(0,0.25,0.5,1.0,2.0 mg/mL)的血紫蛋白纳米粒、血紫蛋白、血红蛋白氧载体HBOC-201分别与巨噬细胞共同孵育6,24 h,与血管内皮细胞共同孵育24 h,采用CCK-8法检测细胞存活率,ELISA法检测血管内皮细胞培养液中一氧化氮及血管细胞黏附因子1水平。结果与结论:(1)血紫蛋白纳米粒电镜下呈现椭球形,有致密外膜,内部质地较为均匀,粒径为(150.12±1.67) nm,分散指数为0.21±0.03,Zeta电位为(-24.54±2.61) mV,半饱和氧分压为(0.97±0.15) kPa,Hill系数为1.49±0.16。(2)孵育6 h,在≤1.0 mg/mL质量浓度范围内,血紫蛋白纳米粒组、血紫蛋白组、HBOC-201组巨噬细胞存活率均在85%以上;在2.0 mg/mL质量浓度下,仅血紫蛋白纳米粒组巨噬细胞存活率在80%以上。孵育24 h,3组巨噬细胞存活率均低于80%,其中血紫蛋白纳米粒组巨噬细胞存活率高于血紫蛋白组、HBOC-201组(P <0.05)。(3)随着药物质量浓度的增加,3组血管内皮细胞存活率降低,在1.0 mg/mL或2.0 mg/mL质量浓度下,血紫蛋白纳米粒组细胞存活率高于血紫蛋白组、HBOC-201组(P <0.05);在相同质量浓度下,血紫蛋白纳米粒组一氧化氮水平高于血紫蛋白组、HBOC-201组(P <0.05);在0.25-2.0 mg/mL质量浓度范围内,血紫蛋白纳米粒组血管细胞黏附因子1水平低于血紫蛋白组、HBOC-201组(P <0.05)。(4)结果表明,经过分子内交联和聚多巴胺/聚乙二醇修饰的血紫蛋白纳米粒在体外具有良好的携氧活性,抗吞噬性能更优,细胞毒性更小。

关键词：红细胞代用品血紫蛋白化学修饰细胞实验血红蛋白氧载体

Studies on the structure and catalytic performance of Cu-Zn-Al catalyst prepared by liquid-phase preparation technology under different heat treatment atmosphere

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Journal of Natural Gas Chemistry 2010年第6期19卷 611-616页

作者： zhihua Gao Wei huang Lihua Yin Kechang Xie Key Laboratory of Coal Science and Technology of Ministry of Education and Shanxi Province Taiyuan University of Technology Taiyuan 030024 Shanxi China

Cu-Zn-Al slurry catalysts were prepared using a complete liquid-phase preparation technology under different heat treatment *** catalysts were characterized using X-ray diffraction,X-ray photoelectron spectroscope,and N2 *** application in the single-step synthesis of dimethyl ether from syngas was also *** results indicate that the type of heat treatment atmosphere has an influence on the Cu species and the Cu0/Cu＋ ratio on the catalyst ***,the final Cu/Zn ratio on the catalyst surface is mainly dependent on the composition and reaction environment of the catalyst and less on the type of heat treatment *** prepared catalysts can suppress sintering of active sites at high temperatures,and the type of heat treatment atmosphere mainly affects the capability of the catalyst for methanol *** catalysts perform best using N2 as the heat treatment atmosphere.

关键词： liquid-phase preparation technology slurry reactor dimethyl ether syngas heat treatment atmosphere

A classification-based method to estimate event-related potentials from single trial EEG

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Science China(Life Sciences) 2012年第1期55卷 57-67页

作者： huang zhihua LI MingHong ZHOU ChangLe MA YuanYe Cognitive Science Department Xiamen UniversityXiamen 361005China Fujian Key Laboratory of the Brain-like Intelligent Systems(Xiamen University) Xiamen 361005China College of Mathematics and Computer Science Fuzhou UniversityFuzhou 350108China Kunming Institute of Zoology Chinese Academy of SciencesKunming 650223China

A novel method based on machine learning is developed to estimate event-related potentials from single trial electroencephalography. This paper builds a basic framework using classification and an optimization model based on this framework for estimating event-related potentials. Then the SingleTrialEM algorithm is derived by introducing a logistic regression model, which could be obtained by training before SingleTrialEM is used, to instantiate the optimization model. The simulation tests demonstrate that the proposed method is correct and solid. The advantage of this method is verified by the comparison between this method and the Woody filter in simulation tests. Also, the cognitive test results are consistent with the conclusions of cognitive science.

关键词： classification spatial-temporal signal model optimization logistic regression SingleTrialEM

A novel single-trial event-related potential estimation method based on compressed sensing

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Neuroscience Bulletin 2013年第6期29卷 788-797页

作者： zhihua huang Minghong Li Shangchuan Yang Yuanye Ma Changle Zhou College of Mathematics and Computer Science Fuzhou University Kunming Institute of Zoology CAS Cognitive Science Department Xiamen University

Cognitive functions are often studied using eventrelated potentials（ERPs）that are usually estimated by an averaging ***,estimation of single-trial ERPs can provide researchers with many more details of cognitive activity than the averaging algorithm.A novel method to estimate single-trial ERPs is proposed in this *** method includes two key ***,singular value decomposition was used to construct a matrix,which mapped singletrial electroencephalographic recordings（EEG）into a low-dimensional vector that contained little information from the spontaneous ***,we used the theory of compressed sensing to build a procedure to restore single-trial ERPs from this low-dimensional *** are sparse or approximately sparse in the frequency *** fact allowed us to use the theory of compressed *** verified this method in simulated and real *** method and dVCA（differentially variable component analysis）,another method of single-trial ERPs estimation,were both used to estimate single-trial ERPs from the same simulated *** demonstrated that our method significantly outperforms dVCA under various conditions of signal-to-noise ***,the single-trial ERPs estimated from the real data by our method are statistically consistent with the theories of cognitive science.

关键词： compressed sensing event-related potentials single-trial electroencephalography singular value decomposition

Thermal safety analysis of aluminum matrix B4C irradiation in-pile

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Nuclear Science and Techniques 2013年第B12期24卷 79-82页

作者： MI Xiangmiao QIAN Dazhi ZHANG zhihua huang Hongwen GUO Haibin Institute of Nuclear Physics and Chemistry China Academy of Engineering Physics Mianyang 621900 China

Aluminum matrix B4C is a new structural material for spent fuel storage and related performances need in-depth research, especially the irradiation-resistance capability. The thermal calculations were completed by using the CFD software to ensure the safety of the in-pile irradiation test. Considering the characteristic of the irradiation project, the thermal safety feature of the in-pile test was analyzed, and the irradiation project was optimized. © 2013, Science Press. All rights reserved.

关键词：安全性分析 B4C 照射铝基乏燃料贮存 CFD软件结构材料安全功能

Regulation of pathological BBB restoration via nanostructured ROS-responsive glycolipid-like copolymer entrapping siVEGF for glioblastoma targeted therapeutics

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Nano Research 2022年第2期15卷 1455-1465页

作者： Lijuan Wen Yu Peng Kai Wang zhihua huang Shiying He Ruiwen Xiong Longping Wu Fengtian Zhang Fuqiang Hu National Engineering Research Center for Modernization of Tranditional Chinese Medicine-Hakka Medical Resources Branch College oj PharmacyGannan Medical UniversityGanzhou 341000China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310058China Department of Orthopedics First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000China Department of Orthopedics Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education Gannan Medical UniversityGanzhou 341000China

Glioblastoma(GBM)is one of the malignant brain tumors with high mortality and no curative *** elevated vascular endothelial growth factor(VEGF)in GBM seriously disrupts the blood brain barrier(BBB)with an increased permeability,resulting in poor outcome and *** interference has shown strong potential to inhibit VEGF expression,thus it is necessary to development an effective and safe gene delivery system possessing the ability to cross the BBB and target GBM *** study aims to explore the anti-GBM effect of angiopep-2(Ap)peptide modified reactive oxygen species(ROS)cleavable thioketal(TK)linked glycolipid-like nanocarrier(CSTKSA)delivering anti-VEGF siRNA(R),termed as Ap-CSTKSA/R *** functionalized modification produced an enhanced cellular uptake and a stronger bio-distribution of Ap-CSTKSA/R complexes in U87 MG cells and brain tumor tissues,***-CSTKSA/R complexes exhibited great superiority in GBM growth inhibition and finally translated into the longest survival period mainly via receptor-mediated targeting delivery,VEGF gene silencing accompanied with remarkable angiogenesis inhibition,and suppressed expression of caveolin-1 which is involved in BBB functional regulation in the occurrence and treatment of *** study indicated that Ap functionalization on ROS-responsive glycolipid-like copolymer exhibits a promising and effective gene delivery platform for GBM targeted treatment.

关键词： glioblastoma ROS-responsive siRNA blood brain barrier copolymer

Regulation of pathological blood-brain barrier for intracranial enhanced drug delivery and anti-glioblastoma therapeutics

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Oncology Research 2021年第5期29卷 351-363页

作者： KAI WANG FENGTIAN ZHANG CHANGLONG WEN zhihua huang ZHIHAO HU YUWEN ZHANG FUQIANG HU LIJUAN WEN Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education Gannan Medical UniversityGanzhou341000China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou310058China Department of Orthopedics First Affiliated Hospital of Gannan Medical UniversityGanzhou341000China Medical College of Soochow University Suzhou215123China Department of Infectious Diseases Ganzhou People’s HospitalGanzhou341000China Ganzhou Key Laboratory of Neuroinflammation Research Gannan Medical UniversityGanzhou341000China

The blood-brain barrier(BBB)is an essential component in regulating and maintaining the homeostatic microenvironment of the central nervous system(CNS).During the occurrence and development of glioblastoma(GBM),BBB is pathologically destroyed with a marked increase in *** to the obstruction of the BBB,current strategies for GBM therapeutics still obtain a meager success rate and may lead to systemic ***,chemotherapy could promote pathological BBB functional restoration,which results in signiﬁcantly reduced intracerebral transport of therapeutic agents during multiple administrations of GBM and the eventual failure of GBM *** effective delivery of intracerebral drugs still faces severe ***,strategies that regulate the pathological BBB to enhance the transport of therapeutic agents across the barrier may provide new opportunities for the effective and safe treatment of *** article reviews the structure and function of BBB in physiological states,the mechanisms underlying BBB pathological fenestration during the development of GBM,and the therapeutic strategies of GBM based on BBB intervention and medicinal drugs transporting across the BBB.

关键词： Blood-brain barrier Physiological Pathological Glioblastoma Intervention

AD-16 Protects Against Hypoxic-Ischemic Brain Injury by Inhibiting Neuroinflammation

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Neuroscience Bulletin 2022年第8期38卷 857-870页

作者： zhihua huang Zhengwei Luo Andrea Ovcjak Jiangfan Wan Nai-hong Chen Wenhui Hu Hong-Shuo Sun Zhong-Ping Feng Department of Physiology Temerty Faculty of MedicineUniversity of TorontoTorontoONM5S 1A8Canada Department of Surgery Temerty Faculty of MedicineUniversity of TorontoTorontoONM5S 1A8Canada State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaNeuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Key Laboratory of Molecular Target and Clinical Pharmacology State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou511436GuangdongChina Leslie Dan Faculty of Pharmacy University of TorontoTorontoONM5S 3M2Canada

Neuroinflammation is a key contributor to the pathogenic cascades induced by hypoxic-ischemic(HI)insult in the neonatal ***-16 is a novel anti-inflammatory compound,recently found to exert potent inhibition of the lipopolysaccharide-induced production of pro-inflammatory and neurotoxic *** this study,we evaluated the effect of AD-16 on primary astrocytes and neurons under oxygen-glucose deprivation(OGD)in vitro and in mice with neonatal HI brain injury in *** demonstrated that AD-16 protected against OGD-induced astrocytic and neuronal cell *** dose post-treatment with AD-16(1 mg/kg)improved the neurobehavioral outcome and reduced the infarct volume with a therapeutic window of up to 6 *** administration reduced the mortality rate and preserved whole-brain morphology following neonatal *** in vitro and in vivo effects suggest that AD-16 offers promising therapeutic efficacy in attenuating the progression of HI brain injury and protecting against the associated mortality and morbidity.

关键词： Neuroinflammation Neonatal hypoxic-ischemic brain injury Neuroprotection AD-16

Satellite-based RAR performance simulation for measuring directional ocean wave spectrum based on SAR inversion spectrum

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Acta Oceanologica Sinica 2010年第4期29卷 13-20页

作者： REN Lin MAO zhihua huang Haiqing GONG Fang Department of Electromagnetic Fields and Microwave Theory Nanjing University of Science and Technology Nanjing 210094 China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China

Some missions have been carried out to measure wave directional spectrum by synthetic aperture radar （SAR） and airborne real aperture radar （RAR） at a low *** them have their own advantages and *** hope that SAR and satellite-based RAR can complement each other for the research on wave properties in the *** this study,the authors aim to simulate the satellite-based RAR system to validate performance for measuring the directional wave *** principal measurements are introduced and the simulation methods based on the one developed by Hauser are adopted and slightly *** enhance the authenticity of input spectrum and the wave spectrum measuring consistency for SAR and satellite-based RAR,the wave height spectrum inversed from Envisat ASAR data by cross spectrum technology is used as the input spectrum of the simulation *** the process of simulation,the sea surface,backscattering signal,modulation spectrum and the estimated wave height spectrum are simulated in each look *** wave spectrum are measured based on the simulated observations from 0 ？ to 360 ？ .From the estimated wave spectrum,it has an 180 ？ ambiguity like SAR,but it has no special high wave number cut off in all the ***,the estimated spectrum is compared with the input one in terms of the dominant wave wavelength,direction and SWH and the results are *** simulation shows that satellite-based RAR should be capable of measuring the directional wave ***,it indicates satellite-based RAR basically can measure waves that SAR can measure.

关键词： satellite-based RAR directional wave spectrum SAR inversion performance simulation