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Proteomic response of Phaeocystis globosa to nitrogen limitation

Journal of Oceanology and Limnology 2024年第1期42卷 141-149页

作者： Haisu LIU Ruiwang WEI Qiangyong LEI Lei cui Songhui LÜ Key Laboratory of Eutrophication and Red Tide Prevention Research Center for Harmful Algae and Marine BiologyJinan UniversityGuangzhou 510632China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China National Engineering Laboratory of Electronic and Information Standardization Shenzhen 518057China

Phaeocystis globosa is an important unicellular eukaryotic alga that can also form *** can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur *** far,the ecophysiology of *** has been investigated by numerous ***,the proteomic response of *** to nitrogen depletion remains largely *** compared four protein preparation methods of *** for two-dimensional electrophoresis(2-DE)(Urea/Triton X-100 with trichloroacetic acid(TCA)/acetone precipitation;TCA/acetone precipitation;Radio Immuno Precipitation Assay(RIPA)with TCA/acetone precipitation;and Tris buffer).Results show that the combination of RIPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect,based on which the proteomic response to nitrogen depletion was studied using *** addition,we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold(P<0.05).Most proteins could not be identified,which might be attributed to the lack of genomic sequences of *** nitrogen limitation,replication protein-like,RNA ligase,and sn-glycerol-3-phosphate dehydrogenase were reduced,which may decrease the DNA replication level and ATP production in *** *** increase of endonucleaseⅢand transcriptional regulator enzyme may affect the metabolic and antioxidant function of *** cells and induce cell *** findings provide a basis for further proteomic study of *** and the optimization of protein preparation methods of marine microalgae.

关键词： Phaeocystis globosa nitrogen limitation proteomic response two-dimensional electrophoresis

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Radiation injury and gut microbiota-based treatment

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Protein & Cell 2024年第2期15卷 83-97页

作者： Weihong Wang Bota cui Yongzhan Nie Lijuan Sun Faming Zhang Department of Microbiota Medicine and Medical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing210011China Department of Microbiotherapy Sir Run Run HospitalNanjing Medical UniversityNanjing 211166China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases Xijing Hospital of Digestive DiseasesFourth Military Medical UniversityXi'an 710032China National Clinical Research Center for Digestive Diseases Xi'an 710032China Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationSchool of MedicineNorthwest UniversityXi'an 710069China

The exposure to either medical sources or accidental radiation can cause varying degrees of radiation injury(RI).RI is a common disease involving multiple human body parts and organs,yet effective treatments are currently *** evidence suggests gut microbiota are closely associated with the development and prevention of various *** article summarizes 10 common types of RI and their possible *** also highlights the changes and potential microbiota-based treatments for RI,including probiotics,metabolites,and microbiota ***,a 5P-Framework is proposed to provide a comprehensive strategy for managing RI.

关键词： microbiome ionizing radiation radiation-induced injury short-chain fatty acids fecal microbiota transplant washed microbiota transplantation war nuclear microbiota medicine

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Estimation of free core nutation parameters and availability of computing options

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Geodesy and Geodynamics 2024年第1期15卷 61-74页

作者： Weiwei Yang Xiaoming cui Jianqiao Xu Qingchao Liu Ming Qin State Key Laboratory of Geodesy and Earth's Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430077China University of Chinese Academy of Sciences Beijing 100049China Hubei Luojia Laboratory Wuhan 430079China

The Earth’s Free Core Nutation(FCN) causes Earth tides and forced nutation with frequencies close to the FCN that exhibit resonance ***-precision superconducting gravimeter(SG) and very long baseline interferometry(VLBI) provide good observation techniques for detecting the FCN ***,some choices in data processing and solution procedures increase the uncertainty of the FCN *** this study,we analyzed the differences and the effectiveness of weight function and ocean tide corrections in the FCN parameter detection using synthetic data,SG data from thirty-one stations,and the 10 celestial pole offset(CPO) *** results show that significant discrepancies are caused by different computing options for a single SG *** stacking method,which results in a variation of0.24-5 sidereal days(SDs) in the FCN period(T) and 10^(3)-10^(4) in the quality factor(Q) due to the selection of the weighting function and the ocean tide model(OTM),can effectively suppress this *** statistical analysis results of synthetic data shows that although different weight choices,while adjusting the proportion of diurnal tidal waves involved,do not significantly improve the accuracy of fitted FCN parameters from gravity *** study evaluated a series of OTMs using the loading correction *** fitting of FCN parameters can be improved by selecting the mean of appropriate OTMs based on the evaluation *** the estimation of the FCN parameters based on the forced nutation,it was found that the weight function P_(1) is more suitable than others,and different CPO series(after 2009) resulted in a difference of 0.4 SDs in the T and of 103 in the *** estimated the FCN parameters for SG(T=430.4±1.5 SDs and Q=1.52×10^(4)±2.5×10^(3)) and for VLBI(T=429.8±0.7 SDs,Q=1.88×10^(4)±2.1×10^(3)).

关键词： Free core nutation Superconducting gravimeter Very long baseline interferometry Weight function Ocean tide model

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Algorithms and statistical analysis for linear structured weighted total least squares problem

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Geodesy and Geodynamics 2024年第2期15卷 177-188页

作者： Jian Xie Tianwei Qiu cui Zhou Dongfang Lin Sichun Long School of Earth Science and Spatial Information Engineering Hunan University of Science and TechnologyXiangtan 411201China College of Science Central South University of Forestry and TechnologyChangsha 410018China National-Local Joint Engineering Laboratory of Geo-spatial Information Technology Hunan University of Science and TechnologyXiangtan 411201China

Weighted total least squares(WTLS)have been regarded as the standard tool for the errors-in-variables(EIV)model in which all the elements in the observation vector and the coefficient matrix are contaminated with random ***,in many geodetic applications,some elements are error-free and some random observations appear repeatedly in different positions in the augmented coefficient *** is called the linear structured EIV(LSEIV)*** kinds of methods are proposed for the LSEIV model from functional and stochastic *** the one hand,the functional part of the LSEIV model is modified into the errors-in-observations(EIO)*** the other hand,the stochastic model is modified by applying the Moore-Penrose inverse of the cofactor *** algorithms are derived through the Lagrange multipliers method and linear *** estimation principles and iterative formula of the parameters are proven to be *** first-order approximate variance-covariance matrix(VCM)of the parameters is also derived.A numerical example is given to compare the performances of our proposed three algorithms with the STLS ***,the least squares(LS),total least squares(TLS)and linear structured weighted total least squares(LSWTLS)solutions are compared and the accuracy evaluation formula is proven to be feasible and ***,the LSWTLS is applied to the field of deformation analysis,which yields a better result than the traditional LS and TLS estimations.

关键词： Linear structured weighted total least squares Errors-in-variables Errors-in-observations Functional modelmodification Stochastic model modification Accuracyevaluation

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User Preference Aware Hierarchical Edge-User Cooperative Caching Strategy

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China Communications 2024年第6期21卷 69-86页

作者： Wu Dapeng Yang Lin cui Yaping He Peng Wang Ruyan School of Communications and Information Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China Advanced Network and Intelligent Connection Technology Key Laboratory of Chongqing Education Commission of China Chongqing 400065China Chongqing Key Laboratory of Ubiquitous Sensing and Networking Chongqing 400065China

The emergence of various new services has posed a huge challenge to the existing network *** improve the network delay and backhaul pressure,caching popular contents at the edge of network has been considered as a feasible ***,how to efficiently utilize the limited caching resources to cache diverse contents has been confirmed as a tough problem in the past *** this paper,considering the time-varying user requests and the heterogeneous content sizes,a user preference aware hierarchical cooperative caching strategy in edge-user caching architecture is *** divide the caching strategy into three phases,that is,the content placement,the content delivery and the content *** the content placement phase,a cooperative content placement algorithm for local content popularity is designed to cache contents *** the content delivery phase,a cooperative delivery algorithm is proposed to deliver the cached *** the content update phase,a content update algorithm is proposed according to the popularity of the ***,the proposed caching strategy is validated using the MovieLens dataset,and the results reveal that the proposed strategy improves the delay performance by at least 35.3%compared with the other three benchmark strategies.

关键词： cooperative caching network delay timevarying popularity user preference

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A novel method for modeling the phase change of iron ore particles in the cohesive zone of a blast furnace

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Particuology 2024年第3期86卷 252-261页

作者： Dianyu E Yingming Wen Jiayun Dan Youyuan Jiang Guangchao Wei Jiaxin cui Jiangxi Provincial Key Laboratory for Simulation and Modelling of Particulate Systems Jiangxi University of Science and TechnologyNanchang330013China International Institute for Innovation Jiangxi University of Science and TechnologyNanchang330013China Ironmaking Plant Xiangtan Iron and Steel Corporation of Hunan HualingXiangtan411101China Schoolof Transportation and Vehicle Enginering Shandong University of TechnologyZibo255000China

Cohesive zone plays a vital role in the stable operation of a blast furnace(BF),yet the complex phase change process of iron ore particles in this zone is still not well *** this study,a novel one-dimensional(1D)unsteady phase change model was developed to elucidate the heat transfer and melting mechanisms of iron ore *** model validation,the effects of several key operating parameters(e.g.,particle diameter,gas velocity,initial temperature)on the phase change behavior of iron ore particles were analyzed,and the joint effect of multiple parameters was *** results show that larger-sized iron ore particles possess lower specific surface areas,which in turn reduces their convective heat absorption ***,the distance from the solid-liquid phase interface to the particle surface increases,thereby slowing down the movement of the phase interface and pro-longing the melting duration of the *** the gas velocity and the initial temperature does not have a significant impact on reducing the duration of the complete melting *** the specified conditions,it is observed that increasing the gas velocity by 3-fold and 9-fold results in a reduction of the melting duration by 2.4%and 8.3%,*** the initial temperature of iron ore particles results in a decrease in the core-to-surface temperature difference,a slower heating rate,and a shorter duration to achieve *** the factors affecting the melting process,the particle diameter is found to be the most significant in terms of the liquid phase precipitation,mushy zone thickness,and core-to-surface temperature difference of iron ore particles.

关键词： Blast furnace Iron ore melting Cohesive zone Heat transfer Phase change

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Cross-linkedα-amylase aggregates on Fe3O4 magnetic nanoparticles modified with polydopamine/polyethyleneimine for efficient hydrolysis of starch

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Particuology 2024年第7期90卷 98-105页

作者： Jiandong cui Xiuming Tang Qingqing Ma Yuyan Chang Qunli Zhang Shiru Jia State Key Laboratory of Food Nutrition and Safety Laboratory of Industrial Fermentation MicrobiologyMinistry of EducationTianjin University of Science and TechnologyTianjin 300457China Key Laboratory of Biomass Chemical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China

In this workα-amylase was immobilized on magnetic Fe3O4 nanoparticles with polyethylenimine(PEI)/polydopamine(PDA)coating or 3-aminopropyl triethoxysilane(APTES)for the first time via adsorption–precipitation–*** with the freeα-amylase,the resultant magnetic cross-linkedα-amylase aggregates(PEI/PDA-M-CLEAs and N-M-CLEAs)exhibited excellent thermal and storage stability as well as pH *** storage at 25°C for 60 days,freeα-amylase only retained 60%of its initial activity,while PEI/PDA-M-CLEAs and N-M-CLEAs retained 80%and 78%of their initial activities,***,N-M-CLEAs and PEI/PDA-M-CLEAs showed good *** 6 repeated uses,PEI/PDA-M-CLEAs and N-M-CLEAs still maintained 65%and 62%of their initial activities,***,PEI/PDA-M-CLEAs and N-M-CLEAs exhibited higher starch hydrolysis efficiency than freeα-*** maximum dextrose equivalent(DE)values of starch hydrolysis by PEI/PDA-M-CLEAs and N-M-CLEAs reached 29.24%and 28.79%within 90 min,***,the maximum DE values of starch hydrolysis by the freeα-amylase was only 27.89%even in 150 *** magnetic cross-linkedα-amylase aggregates could be introduced as effective biocatalyst for industrial applications in production of maltose syrups.

关键词： α-amylase Magnetic nanoparticles Functional modification Magnetic cross-linked enzyme aggregates Hydrolysis of starch

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Co-Sharing Waveform Design for Millimeter-Wave Radar Communication Systems

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China Communications 2024年第6期21卷 305-318页

作者： cui Gaofeng He Mengmin Xu Lexi Wang Changheng Wang Weidong School of Electronic Engineering Beijing University of Posts and TelecommunicationsBeijing 100876China Key Laboratory of Universal Wireless Communications Ministry of EducationBeijing University of Posts and TelecommunicationsBeijing 100876China China United Network Communications Corporation Beijing 100048China

Millimeter-wave(mmWave)radar communication has emerged as an important technique for future wireless ***,the interference between the radar signal and communication data is the main issue that should be considered for the joint radar communication *** this paper,a co-sharing waveform(CSW)is proposed to achieve communication and radar sensing *** eliminate the co-interference between the communication and sensing signal,signal splitting and processing methods for communication data demodulation and radar signal processing are given *** results show that the bit error rate(BER)of CSW is close to that of the pure communication ***,the proposed CSW can achieve better performance than the existing waveforms in terms of range and velocity estimation.

关键词： co-sharing waveform millimeter-wave radar communication radar sensing range and velocity estimation

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Bridging molecular photosensitizer and catalyst on carbon nanotubes toward enhanced selectivity and durability for CO_(2)photoreduction

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Journal of Environmental Sciences 2024年第6期140卷 157-164页

作者： Xiaofeng cui Xueting Wang Lijun Zhao Jixin Wang Tingting Kong Yujie Xiong Anhui Engineering Research Center of Carbon Neutrality The Key Laboratory of Functional Molecular SolidsMinistry of EducationAnhui Laboratory of Molecular-Based MaterialsCollege of Chemistry and Materials ScienceAnhui Normal UniversityWuhu 241002China School of Chemistry and Chemical Engineering Anqing Normal UniversityAnqing 246011China School of Chemistry and Materials Science University of Science and Technology of ChinaHefei 230026China

Homogenous molecular photocatalysts for CO_(2)reduction,especially metal complex-based photosensitizer-catalyst assemblages,have been attracting extensive research interests due to their efficiency and ***,their low durability and recyclability limit practical *** this work,we immobilized the catalysts of metal terpyridyl complexes and the photosensitizer of[Ru(bpy)3]Cl2onto the surface of carbon nanotubes through covalent bonds and electrostatic interactions,respectively,transforming the homogeneous system into a heterogeneous *** characterizations prove that these metal complexes are well dispersed on CNTs with a high loading(ca.12 wt.%).Photocatalytic measurements reveal that catalytic activity is remarkably enhanced when the molecular catalysts are anchored,which is three times higher than that of homogeneous molecular ***,when the photosensitizer of[Ru(bpy)3]Cl2is immobilized,the side reaction of hydrogen evolution is completely suppressed and the selectivity for CO production reaches 100%,with its durability also significantly *** work provides an effective pathway for constructing heterogeneous photocatalysts based on rational assembly of efficient molecular photosensitizers and catalysts.

关键词： durability selectivity catalyst

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Evolutionary Optimization Methods for High-Dimensional Expensive Problems:A Survey

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1092-1105页

作者： MengChu Zhou Meiji cui Dian Xu Shuwei Zhu Ziyan Zhao Abdullah Abusorrah Department of Electrical and Computer Engineering New Jersey Institute of TechnologyNewarkNJ 07102 USA School of Information and Electronic Engineering Zhejiang Gongshang UniversityHangzhou 310018China School of Intelligent Manufacturing Nanjing University of Science and TechnologyNanjing 210094China Institute of Systems Engineering Macao University of Science and TechnologyMacao 999078China School of Artificial Intelligence and Computer Jiangnan UniversityWuxi 214122China School of Information Science and Engineering Northeastern UniversityShenyang 110819China Center of Research Excellence in Renewable Energy and Power Systems Department of Electrical and Computer EngineeringFaculty of EngineeringKing Abdulaziz UniversityJeddah 21589Saudi Arabia K.A.CARE Energy Research and Innovation Center King Abdulaziz UniversityJeddah 21589Saudi Arabia

Evolutionary computation is a rapidly evolving field and the related algorithms have been successfully used to solve various real-world optimization *** past decade has also witnessed their fast progress to solve a class of challenging optimization problems called high-dimensional expensive problems(HEPs).The evaluation of their objective fitness requires expensive resource due to their use of time-consuming physical experiments or computer ***,it is hard to traverse the huge search space within reasonable resource as problem dimension *** evolutionary algorithms(EAs)tend to fail to solve HEPs competently because they need to conduct many such expensive evaluations before achieving satisfactory *** reduce such evaluations,many novel surrogate-assisted algorithms emerge to cope with HEPs in recent *** there lacks a thorough review of the state of the art in this specific and important *** paper provides a comprehensive survey of these evolutionary algorithms for *** start with a brief introduction to the research status and the basic concepts of ***,we present surrogate-assisted evolutionary algorithms for HEPs from four main *** also give comparative results of some representative algorithms and application ***,we indicate open challenges and several promising directions to advance the progress in evolutionary optimization algorithms for HEPs.

关键词： computer optimization evolutionary

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