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Poly immobilized magnetic nanoparticles as new adsorbent for extraction and enrichment of Benzene compounds from water

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Poly immobilized magnetic nanoparticles as new adsorbent for...

河南省化学会2016年学术年会

作者： Shanqi Liu Yanlong Chen Wenfen Zhang zhifen deng Shusheng Zhang School of Chemistry and Molecular Engineering Zhengzhou University at Henan

With 5 kinds of trace benzene compounds in water samples as the object. The effects of the newly synthesized COF magnetic material extraction conditions and in water non polar organic matter adsorption characteristics of extraction, and the adsorption of the substance comparison, solid phase extraction and liquid chromatography analysis of the water five benzene compounds. Determination of low content of organic pollutants in water has certain advantages.

关键词： new adsorbent HPLC benzene compounds

Identification of nephrotic urinary protein

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Identification of nephrotic urinary protein

河南省化学会2016年学术年会

作者： zhifen deng Hang Yuan Zhicong Yang Yanjie Huang Shusheng Zhang College of Chemistry and Molecular Engineering Zhengzhou University Center for Advanced Analysis and Computational Science Zhengzhou University The first affiliated hospital of Henan University of Chinese Medicine

Comparison urinary proteomics is a good way to discover biomarkers related to kidney disease. In this work, 18 proteinuria samples were treated by FASP method, analysed by nano LC-MS/MS. The experimental conditions are as follow: Ekspert nano LC 425-AB SCIEX Triple TOF 6600 system, C18column(Eksigent technologies, particle size 3 μm, 75 μm×15 cm), mobile phase was consist of A(2%Acetnitrile+0.1% Fomic acid) and B(98% Acetnitrile+0.1% Fomic acid), flow rate was 300 n L min-1,the separation was performed by 1D gradient elution(Table 1), duration time was 90 min, scan type TOF-MS(+)-product ion(+) IDA, high sensitivity, TOF-Masses(Da): min 350.0000, max *** source parameters: gas1 8.00, CUR 30.00, ISVF 2200.00, IHT 120;IDA parameters: ion tolerance50.000 m Da, with charge state 2 to 4. Flow rate of loading pump was 3 μL min-1, trap wash 10 min,injection volume was 2 μL. The TIC spectrum was shown in Figure 1. The protein was identified by Protein Pilot TM software 5.0, the database was "human+comtam". The results showed 680 proteins and 18934 distinct peptides were discovered with global FDR(<5%) for disease group, which indicated the method was sensitive, and urine can provide rich information.

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Isolation and detection of resveratrol from the extract of microbial fermentation by LC-MS/MS

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Isolation and detection of resveratrol from the extract of m...

河南省化学会2016年学术年会

作者： Yi Guo zhifen deng Hang Yuan Xue Ma Shusheng Zhang Ruiyong Wang School of Chemistry and Molecular Engineering Zhengzhou University at Henan

Resveratrol is a kind of polyphenolic compound, found in a limited number of plant species such as grape, peanut, and pine. It has been considered as a phytoalexin in plants, and many studies have also shown its health benefits such as antioxidant activities, cancer prevention, blood thinning, and life span extension. Thus it is significant to develop a method for separation and identification of more resveratrol isomers, and study its medicinal value. In this work, a LC-MS/MS method for determination of resveratrol isomers in microbial fermentation extracts was established. The optimization LC-MS/MS parameters are as follow: Shimadzu LC-30 A system coupled with AB SCIEX Q-TRAP 6500 mass spectrometer, C18 Kinetex column(50×2.1 mm, 2.6 μm), column temperature was 40 ℃, mobile phase was consisted with A(100% HO) and B(Methanol/ water, V/V, 80/20), gradient elution(Table 1), total flowrate was 0.2500 m L min, injection volume was 1 μL, MRM scan type, negative mode, curtain gas30, gas1 and gas2 both were set as 55, DP voltage was-70, CE voltage for quantitative Q1/Q3(227/185)was-26, for qualitative Q1/Q3(227/143) was-34. All the samples and standard samples are dissolved with methanol-water 1:1. As the results showed(Figure 1), five isomers of resveratrol were successfully isolated and identified, which are quantitatively more than the literature reported. In conclusion, this method can be applied for the separation and determination of resveratrol from the fermentation broth.

关键词： resveratrol LC-MS/MS microbial extract

Determination of albendazole and its metabolites in animal tissues by HPLC-FLD

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Determination of albendazole and its metabolites in animal t...

河南省化学会2016年学术年会

作者： Yanlong Chen Huifang Du Gaigai Xu Shengnan Zhao Ningpeng Wu zhifen deng Wenfen Zhang Shusheng Zhang Veterinary Drug Supervision & Animal Husbandry Bureau of Henan Province College of Chemistry and Molecular Engineering Zhengzhou University

Food safety has as a Global Concern problem, and illicit drugs in food detection and drug residual problem gradually become a hot issue. Albendazole is anthelmintic benzimidazole most widely used as veterinary medicine for the treatment of infections caused *** of albendazole has potential harm to human body, so many countries have regulations maximum residue and the method has established for detecting albendazole and its metabolites in animal tissues. However, most of detection methods had serious impurity interference, so it is necessary to establish a new method for sample preparation. In this paper, a high-performance liquid chromatography with fluorescence detection(HPLC-FLD) method, using acidic alumina and MCX solid-phase extraction(SPE) cartridges was developed for extracting and purifying the albendazole and its metabolites in animal *** limits of detection(LOD) were about 1-10 ng/m L, the limits of quantification(LOQ) were 4.0 and 20.0ng/m L for albendazole and its metabolites. The intraday and interday RSDs(n=6) of peak areas and retention times were in the range of 2.0.-15%. In addition, overall recoveries through the extraction and SPE purification ranged from 78.4% to 102.8%. Compared with others detection methods, the two cartridges(acidic alumina and MCX) in series connection was proposed SPE-HPLC–FLD method had highly effective and good purification effects for trace analysis of the albendazole and its metabolites.

关键词： albendazole solid-phase extraction(SPE)

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不同激素处理对九龙山杜鹃种子萌发的影响

分子植物育种 2022年第11期20卷 3759-3767页

作者：刘杰邓志芬彭科升王冉王灵军黎鸿陈中阳贵州师范大学生命科学学院贵阳550001 贵州师范大学贵州省植物生理与发育调控重点实验室贵阳550001 贵州省百里杜鹃管理区科研所毕节551700

为研究提高新品种九龙山杜鹃种子的发芽率的途径与方法,本试验以贵州百里杜鹃地区采集的野生九龙山杜鹃种子为试验材料,通过不同浓度的NAA、GA_(3)、6-BA、IAA四种单一激素、不同浓度的两种混合溶液对种子进行处理,研究不同处理对九龙... 详细信息

为研究提高新品种九龙山杜鹃种子的发芽率的途径与方法,本试验以贵州百里杜鹃地区采集的野生九龙山杜鹃种子为试验材料,通过不同浓度的NAA、GA_(3)、6-BA、IAA四种单一激素、不同浓度的两种混合溶液对种子进行处理,研究不同处理对九龙山种子发芽率与成苗率的影响。结果表明,用NAA溶液处理对九龙山种子的萌发有抑制作用;低浓度下的GA_(3)溶液、IAA溶液对九龙山杜鹃种子萌发均有促进作用,其中1000 mg/L的GA_(3)溶液促进萌发的作用最为明显,发芽时间缩短,相比对照组发芽率提高;一定范围内的6-BA溶液对九龙山杜鹃种子的萌发没有显著的影响,浓度过高会完全抑制其萌发;浓度为1 mg/L的IAA溶液能促进九龙山杜鹃种子的萌发,浓度低于或高于1 mg/L对九龙山种子萌发都有抑制作用;NAA与6-BA、NAA与GA_(3)两种混合激素溶液均对九龙山杜鹃种子萌发均有抑制作用。通过不同浓度的不同激素溶液对九龙山杜鹃种子进行处理,旨在为新品种开花较晚的九龙山杜鹃的开发利用提供科学依据。

关键词：九龙山杜鹃(Rhododendron jiulongshanense) 种子发芽率成苗率

长江经济带环境政务新媒体的传播图景研究——以11个省级环境政务微博为例

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环境科学与管理 2024年第6期49卷 19-24页

作者：邓倩徐志芬兰祥敏河海大学公共管理学院江苏南京211100 长江保护与高质量发展智库江苏南京211100

在进一步推进长江经济带高质量发展背景下,环境政务新媒体建设对长江经济带生态环境治理体系和治理能力现代化具有重要意义。文章基于计算传播方法,采集了2019年-2021年长江经济带11个省级环境政务微博的全量数据,结合更新数量与频率、... 详细信息

在进一步推进长江经济带高质量发展背景下,环境政务新媒体建设对长江经济带生态环境治理体系和治理能力现代化具有重要意义。文章基于计算传播方法,采集了2019年-2021年长江经济带11个省级环境政务微博的全量数据,结合更新数量与频率、内容原创度、主题与高频词、粉丝数量和转发、点赞、评论数量指标呈现长江经济带环境政务微博的传播图景。从信息发布、互动交流和办事服务三个面向评估长江经济带环境政务微博发展现状并给予相应对策,以期提升长江经济带环境政务微博影响力,以高质量生态环境支撑长江经济带高质量发展。

关键词：长江经济带政务新媒体生态环境计算传播

Mixolab参数与青稞全粉饼干品质相关性研究

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美食研究 2024年第1期41卷 78-85页

作者：邓晓青潘志芬丁捷李想肖鑫鑫潘云莲四川旅游学院烹饪学院四川成都610100 中国科学院成都生物研究所四川成都610213

为探究Mixolab参数与青稞全粉饼干品质之间的关系,通过Mixolab仪测定13份青稞全粉,并分析Mixolab参数与青稞全粉饼干营养成分、体外淀粉水解特性及饼干质构特性之间的关联性。结果显示:青稞材料间的流变特性差异明显,其中,C1(characteri... 详细信息

为探究Mixolab参数与青稞全粉饼干品质之间的关系,通过Mixolab仪测定13份青稞全粉,并分析Mixolab参数与青稞全粉饼干营养成分、体外淀粉水解特性及饼干质构特性之间的关联性。结果显示:青稞材料间的流变特性差异明显,其中,C1(characteristic torque 1)值与直链淀粉、总淀粉、快速消化淀粉(rapid digested starch,RDS)和体外血糖生成指数(extracorporeal glycemic index,eGI)呈显著负相关,而与β-葡聚糖、蛋白质、脆性、硬度和咀嚼性间呈显著正相关。C3(characteristic torque 3)值则正好相反,与直链淀粉、RDS和eGI间呈显著正相关,与β-葡聚糖、蛋白质、脆性、硬度和咀嚼性呈显著负相关。由此可见Mixolab参数吸水率、C1和C3可以作为预测青稞全粉饼干的营养成分、淀粉水解特性和质构特性的重要参数。这为青稞品种选育和青稞产品加工原料选择提供方便的检测方法。

关键词：青稞营养成分 Mixolab仪水解特性质构特性相关性分析

GA_(3)浸种处理对五种晚花杜鹃种子萌发的影响

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广西植物 2021年第6期41卷 946-952页

作者：陈婷邓志芬龙宇庭蒙俊霞刘杰乙引贵州师范大学贵州省植物生理与发育调控重点实验室贵阳550001 国家林业局西南喀斯特山地生物多样性保护重点实验室贵阳550001 贵州师范大学生命科学学院贵阳550001

晚花杜鹃(late flower Rhododendron)是一类花期较晚的杜鹃品种,具有较高的观赏价值,被广泛应用于庭院种植和园林绿化。随着旅游以及经济发展的需要,开发和利用晚花杜鹃资源显得非常迫切。自然条件下晚花杜鹃萌发率相对较低,因此为提高... 详细信息

晚花杜鹃(late flower Rhododendron)是一类花期较晚的杜鹃品种,具有较高的观赏价值,被广泛应用于庭院种植和园林绿化。随着旅游以及经济发展的需要,开发和利用晚花杜鹃资源显得非常迫切。自然条件下晚花杜鹃萌发率相对较低,因此为提高晚花杜鹃萌发率,探究不同浓度赤霉素(GA_(3))处理对五种杜鹃种子萌发的影响,该文以五种晚花杜鹃(小白杜鹃、大白杜鹃、桃叶杜鹃、长蕊杜鹃和九龙山杜鹃)为实验材料,通过不同浓度(0、300、400、500、600、700 mg·L^(-1))的GA_(3)对五种晚花杜鹃种子进行24 h浸种处理,测定其发芽指数、发芽势、发芽率以及成苗率等指标,分别确定五种杜鹃种子萌发的最适GA_(3)浓度,并对相同处理下五种杜鹃种子的萌发率和成苗率进行比较。结果表明:(1)GA_(3)浸种对五种晚花杜鹃种子萌发具有促进作用,在适当GA_(3)浓度下五种杜鹃种子的发芽指数、发芽势和发芽率均显著高于对照组,萌发时滞、萌发高峰期和持续萌发时间均较对照组浓度相对缩短。(2)大白杜鹃、桃叶杜鹃和九龙山杜鹃种子在GA_(3)浓度为600 mg·L^(-1)处理时各项萌发指标相对较好;长蕊杜鹃以GA_(3)浓度为700 mg·L^(-1)浸种处理萌发效果相对较好;小白杜鹃以GA_(3)浓度为400和700 mg·L^(-1)处理最好。因此,在杜鹃的栽培中,可以采用赤霉素GA_(3)处理法提高种子发芽率,缩短萌发时间。

关键词：晚花杜鹃种子繁殖发芽指数发芽势发芽率成苗率

通过权重共表达网络分析胰腺癌转移的特异基因网络

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贵州师范大学学报（自然科学版） 2020年第3期38卷 39-44,89页

作者：唐婧程美玲肖玉凤邓志芬李菁贵州师范大学贵州省植物生理与发育重点实验室生命科学学院贵州贵阳550025

胰腺癌是一种高度恶性的消化道肿瘤,临床表现不具有特征性,在早期难诊断易转移,发展迅速;胰腺癌转移率高,且转移后难以治愈进而导致死亡率升高。因此相比胰腺癌未转移来说,研究确定预测胰腺癌转移、转移后的新治疗方法及治疗的潜在靶标... 详细信息

胰腺癌是一种高度恶性的消化道肿瘤,临床表现不具有特征性,在早期难诊断易转移,发展迅速;胰腺癌转移率高,且转移后难以治愈进而导致死亡率升高。因此相比胰腺癌未转移来说,研究确定预测胰腺癌转移、转移后的新治疗方法及治疗的潜在靶标尤为迫切并具有重要意义。而且近两年兴起的基于基因之间相关关系的共表达网络分析法,为多种复杂疾病的研究提供了新的线索和思路。我们应用TCGA数据库中的大量胰腺癌转录组数据,筛选胰腺癌转移差异表达基因,使用共表达网络分析法(WGCNA)筛选胰腺癌转移后,基因间出现一个52个差异表达基因构建的新共表达网络模块,且挖掘到其中ASIC4,GLDC,MMD2,CTNNA2,FOXC4-AS1,SNHG19和BCAN等11个枢纽基因。我们推测认为它们为胰腺癌转移提供了新的潜在诊断、治疗靶标。同时KEGG富集分析发现,该新基因网络模块内基因富集于炎症介质对色氨酸通道的调节,卵母细胞减数分裂,乙醛酸和二元羧酸盐代谢和甘氨酸、丝氨酸和苏氨酸代谢等4条代谢通路。

关键词：胰腺癌转移 WGCNA 基因网络差异基因