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中华医学杂志(英文版) 2024年第16期137卷 1976-1978页

作者： Ma Xiuwei Zhuang Lu Ma Wenhao Li Jun Wang Xiaodong Li Zhongqiu Jiang Xinyang Wang Yongxia Du Ying Zhang Yingqian He Fang Zhu Zhiming Du Shaopeng Xu Juan Gu Ruijie Zhang Yanping Zhang Shan Li Ting Yang Xiao Zhang Sheng Zhu Lina Li Qiuping Dong Xiaoyan Wu Xiaobing feng zhichun Department of Neurodevelopment Faculty of Pediatrics The Seventh Medical Center of Chinese PLA General Hospital Beijing China National Engineering Laboratory for Birth Defects Prevention and Control of Key Technology Beijing China Research and Development Division Genecradle Therapeutics Inc. Beijing China Medical Division Genecradle Therapeutics Inc. Beijing China Department of Pharmacy Medical Supplies Center of Chinese PLA General Hospital Beijing China Beijing Key Laboratory of Pediatric Organ Failure Beijing China

Hyperbaric oxygen treatment promotes neural stem cell proliferation in the subventricular zone of neonatal rats with hypoxic-ischemic brain damage

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Neural Regeneration Research 2013年第13期8卷 1220-1227页

作者： zhichun feng Jing Liu Rong Ju Department of Neonatology & Neonatal Intensive Care Unit Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command Department of Neonatology Chengdu Women's & Children's Central Hospital

Hyperbaric oxygen therapy for the treatment of neonatal hypoxic-ischemic brain damage has been used clinically for many years, but its effectiveness remains controversial. In addition, the mechanism of this potential neuroprotective effect remains unclear. This study aimed to investigate the influence of hyperbaric oxygen on the proliferation of neural stem cells in the subventricular zone of neonatal Sprague-Dawley rats （7 days old） subjected to hypoxic-ischemic brain damage. Six hours after modeling, rats were treated with hyperbaric oxygen once daily for 7 days. Immunohistochemistry revealed that the number of 5-bromo-2＇-deoxyuridine positive and nestin positive cells in the subventricular zone of neonatal rats increased at day 3 after hypoxic-ischemic brain damage and peaked at day 5. After hyperbaric oxygen treatment, the number of 5-bromo-2＇- deoxyuddine positive and nestin positive cells began to increase at day 1, and was significantly higher than that in normal rats and model rats until day 21. Hematoxylin-eosin staining showed that hyperbaric oxygen treatment could attenuate pathological changes to brain tissue in neonatal rats, and reduce the number of degenerating and necrotic nerve cells. Our experimental findings indicate that hyperbaric oxygen treatment enhances the proliferation of neural stem cells in the subventricular zone of neonatal rats with hypoxic-ischemic brain damage, and has therapeutic potential for promoting neurological recovery following brain injury.

关键词： neural regeneration brain injury neonatal hypoxic-ischemic encephalopathy hypoxic-ischemicbrain damage hyperbaric oxygen neural stem cells neurons proliferation subventricular zone neonatal rats nestin grants-supported paper neuroregeneration

Intestinal Microbiota in Early Life and Its Implications on Childhood Health

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Genomics, Proteomics & Bioinformatics 2019年第1期17卷 13-25页

作者： Lu Zhuang Haihua Chen Sheng Zhang Jiahui Zhuang Qiuping Li zhichun feng Affiliated Bayi Children's Hospital The Seventh Medical Center of PLA General Hospital National Engineering Laboratory for Birth Defects Prevention and Control of Key Technology Beijing Key Laboratory of Pediatric Organ Failure The First Clinical Academy of Dalian Medical University College of the Environment Northeast Normal University

Trillions of microbes reside in the human body and participate in multiple physiological and pathophysiological processes that affect host health throughout the life cycle. The microbiome is hallmarked by distinctive compositional and functional features across different life *** evidence has shown that microbes residing in the human body may play fundamental roles in infant development and the maturation of the immune system. Gut microbes are thought to be essential for the facilitation of infantile and childhood development and immunity by assisting in breaking down food substances to liberate nutrients, protecting against pathogens, stimulating or modulating the immune system, and exerting control over the hypothalamic–pituitary–adrenal *** review aims to summarize the current understanding of the colonization and development of the gut microbiota in early life, highlighting the recent findings regarding the role of intestinal microbes in pediatric diseases. Furthermore, we also discuss the microbiota-mediated therapeutics that can reconfigure bacterial communities to treat dysbiosis.

关键词： Intestinal microbiota Immunity Gut-brain axis Early life Diseases Microbiota manipulation

Staphylococcus epidermidis meningitis in combination with acute disseminated encephalomyelitis A case report

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Neural Regeneration Research 2009年第11期4卷 843-845页

作者： Xin Chen Haijin Chen Zhiqin Liu Cailing Chen Fenping Luo zhichun feng Department of Pediatrics Bayi Children's Hospital Affiliated to Beijing Military Region General Hospital Beijing 100710 China Department of Nuclear Medicine Beijing Military Region General Hospital Beijing 100710 China

A nine-year-old girl with Staphylococcus epidermidis meningitis combined with acute disseminated encephalomyelitis （ADEM） was enrolled in the present study, and exhibited sustained fever and headache for 5 days and a single seizure episode. Meningeal irritation and disturbance of consciousness were apparent. Cerebrospinal fluid examination revealed increased protein level and pleocytosis. Cerebrospinal fluid culture demonstrated Staphylococcus epidermidis growth. The patient was treated with intravenous cefotaxime, intravenous dexamethasone, and decreasing intracranial pressure for 3 weeks. Seizures and fever symptoms improved, but disturbance of consciousness, muscle weakness, and bilateral limb paresis were significant. IgG index was elevated and oligoclonal bands were positive in the cerebrospinal fluid. Magnetic resonance imaging demonstrated high T2-weighted signals in subcortical white matter, which were consistent with ADEM. High-dose methylprednisolone for 3 days and a 6-day pulse therapy with immunoglobulins resulted in improved clinical symptoms and cerebrospinal fluid examination. The patient＇s temperature dropped to normal, and the headache disappeared. A Staphylococcus epidermidis infection associated with ADEM is uncommon in children. Results suggested that Staphylococcus epidermidis infection could be a pathogenic factor for ADEM, and ADEM is a complication of Staphylococcus epidermidis meningitis.

关键词： acute disseminated encephalomyelitis Staphylococcus epidermidis infection children

低渗肠内喂养减轻坏死性小肠结肠炎肠上皮损伤及其机制研究

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首都食品与医药 2024年第7期31卷 12-15页

作者：余梦楠刘钢黄柳明封志纯解放军总医院第七医学中心出生缺陷防控关键技术国家工程实验室儿童器官功能衰竭北京市重点实验室北京100700

目的研究低渗肠内喂养减轻新生大鼠坏死性小肠结肠炎(NEC)肠道上皮损伤的作用,为新防治措施提供理论基础。方法为新生12小时内SD大鼠编号,交替随机将其分为对照组、标准配方奶喂养组和低渗配方奶喂养组,每组20只。通过人工灌胃、脂多糖... 详细信息

目的研究低渗肠内喂养减轻新生大鼠坏死性小肠结肠炎(NEC)肠道上皮损伤的作用,为新防治措施提供理论基础。方法为新生12小时内SD大鼠编号,交替随机将其分为对照组、标准配方奶喂养组和低渗配方奶喂养组,每组20只。通过人工灌胃、脂多糖胃注(4mg/kg/d)及低氧刺激复制NEC大鼠模型。标准及低渗配方奶渗透压分别为256mOsm/kg和128mOsm/kg。48小时后活杀取材回肠。采用苏木精-伊红染色、电镜等技术研究肠内喂养渗透压对大鼠肠道上皮的影响;采用蛋白质免疫印迹法研究渗透压对肠道紧密连接蛋白表达的影响。结果与对照组相比,标准组及低渗组大鼠体重明显降低,差异有统计学意义(P低渗组>标准组,有统计学差异(P<0.05)。结论NEC病变可出现肠绒毛脱落、缩短、紧密连接长度增加,紧密连接蛋白表达降低。低渗肠内喂养能减轻肠上皮损伤,可能机制为减轻肠道紧密连接蛋白的破坏。

关键词：坏死性小肠结肠炎渗透压肠损伤大鼠

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重视支气管肺发育不良定义同质化问题

中华围产医学杂志 2022年第6期25卷 412-415页

作者：封志纯解放军总医院第七医学中心儿科医学部北京100700

支气管肺发育不良(bronchopulmonary dysplasia,BPD)是影响早产儿生命和生存质量的主要疾病之一。BPD的概念随着人们对疾病认知水平的提高及治疗手段的改进而不断更新。由于各个地区、国家使用的BPD定义不一致,BPD的发病率差异很大,严... 详细信息

支气管肺发育不良(bronchopulmonary dysplasia,BPD)是影响早产儿生命和生存质量的主要疾病之一。BPD的概念随着人们对疾病认知水平的提高及治疗手段的改进而不断更新。由于各个地区、国家使用的BPD定义不一致,BPD的发病率差异很大,严重阻碍了药物临床试验的研究进程。因此,BPD定义的标准化、同质化迫在眉睫。BPD定义的同质化可以使诊断标准达成一致,从而使治疗有的放矢,避免医源性损伤。因此,需要重新审视BPD定义中存在的问题。

关键词：支气管肺发育不良婴儿,早产

Study of the incidence of hyperuricemia in young males’population with rapid entry into the plateau of 4500m

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Frigid Zone Medicine 2022年第1期2卷 41-44页

作者： Chang'e Liu Yingyue Liu Xiaoyang Hong zhichun feng Department of Nutrition Seventh Medical Center of PLA General HospitalBeijing 100700China Pediatric Intensive Care Unit Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510515China

Objective:To study the incidence and risk factors of hyperuricemia in young males who rapid entered into the plateau of 4500 ***:The study contained 390 males aged 18-35 years(21.6±2.5 years),who rapidly entered the plateau with an altitude of 4500 *** to their basic level of uric acid(UA),they were divided into two groups,high uric acid(HUA)group and normal uric acid(NUA)*** characteristics and physiological index,such as the body weight and the height,of them were *** the test of the biochemical indicators,the venous blood samples were collected at the altitude of 4500 m in the *** count of blood cells,blood urea nitrogen(BUN),serum creatinine(SCR),lactate dehydrogenase(LDH),total bilirubin(TBIL),direct bilirubin(DBIL)and indirect bilirubin(IDBIL)were compared between the two ***:The incidence of hyperuricemia was 65.1%(254/390)at 4500 *** the altitude of 4500 m,the mean hemoglobin concentration(MCHC)of red blood cells in the HUA group was significantly lower than that in the NUA ***(HGB),mean red blood cell volume(MCV),TBIL,IDBIL,BUN,SCR and LDH in the HUA group were significantly higher than those in the NUA group,though without statistically significant differences in the other ***,multivariate analysis showed at the altitude of 4500 m,the risk of HUA increased by 0.982,1.038 and 1.045 times when MCHC decreased by one unit and TBIL and SCR increased by one unit,***:The incidence of hyperuricemia was high of 65.1%rush entry into the plateau of young *** MCHC and elevated TBIL and SCR were independent risk factors for hyperuricemia when rapid enter into 4500 m.

关键词： rapid entry the plateau hyperuricemia renal function

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新生儿经鼻高流量氧疗应用指南(2023)

中国循证医学杂志 2024年第4期24卷 385-404页

作者：黄益赵静滑心恬罗可人史源林振浪唐军封志纯母得志中国医师协会新生儿科医师分会循证专业委员会四川大学华西第二医院新生儿科成都610041 出生缺陷与相关妇儿疾病教育部重点实验室成都610041 重庆医科大学附属儿童医院新生儿科重庆400014 温州医科大学附属第二医院浙江温州325000 中国人民解放军总医院儿科医学部北京100007 中国人民解放军总医院第七医学中心儿科医学部研究所北京100007 出生缺陷防控关键技术国家工程实验室北京100007 儿童器官功能衰竭北京市重点实验室北京100007 不详

经鼻高流量(HFNC)氧疗,作为重要的无创呼吸支持模式之一,因为其舒适、易于启动、鼻损伤发生率低等优点,越来越多地应用于呼吸衰竭的危重新生儿。其使用优势、适应证及风险等也是近年研究的热点。基于现有证据,本指南采用证据推荐分级评... 详细信息

经鼻高流量(HFNC)氧疗,作为重要的无创呼吸支持模式之一,因为其舒适、易于启动、鼻损伤发生率低等优点,越来越多地应用于呼吸衰竭的危重新生儿。其使用优势、适应证及风险等也是近年研究的热点。基于现有证据,本指南采用证据推荐分级评估、制订与评价方法(GRADE),并广泛征求新生儿科医生、呼吸治疗师、护理专家及循证医学等专家意见后制订。本指南针对9个关键问题提出了24条建议,旨在成为临床实践中HFNC氧疗的证据和参考来源,使更多的新生儿及其家庭从HFNC中获益。

关键词：氧气吸入疗法鼻导管高流量指南新生儿

PIP, Not FiO<sub>2</sub>Regulates Expression of MMP-9 in the Newborn Rabbit VILI with Different Mechanical Ventilation Strategies

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Chinese Medicine 2013年第4期4卷 137-147页

作者： Shaodong Hua Xiaoying Zhang Shengli An Xiuxiang Liu zhichun feng Department of Biostatistics South Medical University Guangzhou China Department of Pediatrics BaYi Children’s Hospital of the General Military Hospital of Beijing PLA Beijing China The Hospital Affilicated Binzhou Medicall University Binzhou China

Background: Results from experimental and clinical studies have shown that mechanical ventilation or/and hyperoxia may aggravate a pre-existing lung injury or even cause lung injury in healthy lungs by affecting the expression of MMP-9, but the MMP-9 effects are controversial. How are MMP-9 regulated when multicausative factors of injury such as different FiO2, PIP, and respiratory time (RT) impose simultaneously on lungs? Methods: Newborn New Zealand white rabbits were randomly allocated to an unventilated air control group or to one of the 2 × 3 × 3 ventilation strategies by using a factorial design, with different FiO2, PIP, and RT. Then, lung wet-to-dry ratio (W/D), lung histopathology scores, transmission electron microscope, and cells in BALF were analyzed in these different groups. MMP-9 levels were studied by immunohistochemistry and ELISA. Results: MMP-9 levels were significantly different among 3 PIP ventilation regimes (F = 7.215) and MPIP group was the highest among 3 PIP groups. The lung histopathology score in 100% oxygen was significantly higher than in 45% oxygen group (F = 9.037) and MPIP group was the lowest among 3 PIP groups (F = 57.515) and RT 6 h was more serious than RT 1 h. MMP-9 positively correlated with monocytes, but negatively correlated with neutrophils and lung injury histopathology scores. Conclusions: Different PIP and FiO2 exert simultaneously on newborn lung in newborn rabbits ventilation, only mechanical stretch stimulation affects MMP-9 synthesis. Advisable mechanical stretch can promote MMP-9 expression and has protective role in lung in VILI. HPIP causes barotraumas and LPIP induces atelectrauma.

关键词： Mechanical Ventilation Lung Injury Matrix Metalloproteinase Newborn Rabbit Fraction of Inspired Oxygen Peak Inspiratory Pressure

RNA Interference Mediated Silencing of Tissue Factor in Human Umbilical Vein Endothelial Cells

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Open Journal of Obstetrics and Gynecology 2018年第5期8卷 477-484页

作者： Xiaojuan Yin Zhu Chen Weiqin Zhou Wen Tang zhichun feng Bayi Children’s Hospital Affiliated to Army General Hospital of People’s Liberation Army Beijing China

Objective: To construct to silence tissue factor (TF) expression in Human Umbilical Vein Endothelial Cells (HUVECs) for providing evidence of the gene therapy and prevention of coagulation dysfunction in placental abruption (PA) neonates. Methods: Cultured HUVECs in vitro were divided into control group and PA group, each including non-prevention, scramble siRNA and TF-siRNA subgroups. pENTRTM/U6-shRNA/TF vector expressing TF were constructed and transfected into HUVECs. The mRNA expression of TF was tested with RT-PCR, and TF protein expression was detected with immunofluorescence staining. Results: Monolayer HUVECs with short-rod and short spindle shaped were adherent to the bottom, forming paving stone arrangement. The TF mRNA expression was significantly different between control group and PA group (P 0.01) and among different subgroups (P 0.01). In non-prevention subgroups, significant difference was observed in TF mRNA expression between control group and PA group (P 0.05). Significant difference in TF mRNA expression was found in false-prevention subgroups (P 0.05). The TF mRNA expression was markedly different among different subgroups in control (P 0.01), and the similar result among different PA subgroups (P 0.01). In both control and PA groups, the TF mRNA expression was the lowest after TF silencing. Immunofluorescence staining showed high TF expression in HUVECs in false-prevention subgroups. In scramble siRNA group, the TF protein expression reduced as compared to non-prevention group and reduced dramatically after TF silencing as compared to control. Conclusion: pENTRTM/U6-TF-shRNA is able to significantly inhibit the TF mRNA and protein expression in HUVECs from healthy neonates and PA neonates.

关键词： Neonate Tissue Factor Placental Abruption