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Chinese Science Bulletin 2008年第15期53卷 2307-2314页

作者： YE GuiXiang CAO zhiqiang LIN Lin chen DaYong LIU WenGuang School of Materials Science and Engineering Tianjin Key Laboratory of Composite and Functional Materials Tianjin UniversityTianjin 300072 China

Previously we successfully prepared poly(vinyldiaminotriazine)(PVDT)-based non-viral vectors which complexed plasmid DNA via hydrogen bonding with adenine-thymine base pairs. In this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events: the aggregation induced by the insta-bility of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin Ⅲ were used to inhibit clathrin- and caveolae-me- diated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by (i) a more en-ergy dependent process and (ii) a much slow transfection-effective internalization.

关键词：聚乙烯络合物氢键细胞提取

基于Th1/Th2平衡探讨升阳益胃汤对膜性肾病大鼠足细胞的影响

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中国实验方剂学杂志 2024年第16期30卷 60-67页

作者：吴媛张学琴安恒通王帅杰王开爽赵亚云陈志强河北中医药大学研究生院石家庄050200 河北中医药大学第一附属医院/河北省中医院石家庄050011

目的:基于辅助性T细胞1(Th1)/辅助性T细胞2(Th2)免疫平衡探讨升阳益胃汤抑制膜性肾病大鼠肾小球足细胞的损伤凋亡分子机制。方法:40只SD大鼠采用尾静脉注射羊抗大鼠肾刷状缘抗体(Fx1A)血清方法建造膜性肾病大鼠模型,将造模成功的大鼠分... 详细信息

目的:基于辅助性T细胞1(Th1)/辅助性T细胞2(Th2)免疫平衡探讨升阳益胃汤抑制膜性肾病大鼠肾小球足细胞的损伤凋亡分子机制。方法:40只SD大鼠采用尾静脉注射羊抗大鼠肾刷状缘抗体(Fx1A)血清方法建造膜性肾病大鼠模型,将造模成功的大鼠分为升阳益胃汤低剂量组、高剂量组及盐酸贝那普利组,健康组大鼠10只作为正常组,各组分别予以对应药物进行灌胃,每天1次,连续干预6周。干预结束前,考马斯亮蓝法检测各组大鼠24 h尿蛋白含量;干预结束后麻醉后腹主动脉取血,自动生化仪检测各组大鼠血清中血肌酐(Crea)、血尿素(UREA)、总胆固醇(TC)含量水平;免疫荧光染色观察各组大鼠肾组织免疫球蛋白G(IgG)、补体C3沉积情况;免疫组化(IHC)检测各组大鼠肾组织中突触足蛋白(Synaptopodin)、足细胞裂孔膜蛋白(Nephrin)、足萼蛋白(Podocalyxin)的表达水平;蛋白免疫印迹法(Western blot)检测各组大鼠肾组织中Synaptopodin、Nephrin、Podocalyxin、B细胞淋巴瘤-2基因(Bcl-2)、Bcl-2基因相关启动子(Bad)、Bcl-2相关X蛋白(Bax)、γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)、T细胞特异性转录因子(T-bet)、GATA结合蛋白-3(GATA-3)的表达水平。结果:与正常组比较,模型组大鼠血清中总胆固醇水平明显升高(P<0.05),24 h尿蛋白水平显著升高(P<0.01);肾组织存在明显C3、IgG沉积;足细胞标志蛋白Synaptopodin、Nephrin、Podocalyxin免疫组化半定量表达明显降低(P<0.05);Western blot检测肾组织Synaptopodin、Nephrin、Podocalyxin蛋白表达水平明显降低(P<0.05),Bax、Bad蛋白表达明显升高(P<0.05),Bcl-2蛋白表达明显降低(P<0.05),IL-4、GATA3蛋白明显升高(P<0.05),IFN-γ、T-bet蛋白水平明显降低(P<0.05)。与模型组比较,各治疗组总胆固醇水平明显降低(P<0.05),24 h尿蛋白水平显著降低(P<0.01);IgG、C3沉积明显减少;足细胞标志蛋白Synaptopodin、Nephrin、Podocalyxin免疫组化半定量表达均明显升高(P<0.05);Synaptopodin、Nephrin、Podocalyxin蛋白表达均明显升高(P<0.05),Bax、Bad蛋白表达明显降低(P<0.05),Bcl-2蛋白表达明显升高(P<0.05),IL-4、GATA3蛋白明显降低(P<0.05),IFN-γ、T-bet蛋白水平明显升高(P<0.05)。结论:升阳益胃汤可能通过调节Th1/Th2免疫平衡减少膜性肾病大鼠肾小球足细胞凋亡,缓解足细胞损伤,减轻肾损伤。

关键词：膜性肾病足细胞升阳益胃汤免疫平衡

IMF production and symmetry energy in heavy ion collisions near Fermi energy

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Nuclear Science and Techniques 2013年第5期24卷 1-6页

作者： WADA Roy HUANG Meirong LIN Weiping LIU Xingquan ZHAO Minghui chen zhiqiang Institute of Modern Physics Chinese Academy of Science University of Chinese Academy of Sciences

The symmetry energy at the time of the production of intermediate mass fragments(IMFs) is studied using experimentally observed IMF multiplicities combined with quantum statistical model calculations(QSM of Hahn and St cker).The ratios of difference in chemical potentials between neutrons and protons relative to the temperature,(μn-μp)/T,and the double ratio temperature,T,were extracted experimentally in the reactions of64,70Zn,64Ni+58,64Ni,112,124Sn,197Au,232Th at 40A *** extracted(μn-μp)/TTscales linearly with δNN,where δNN is the asymmetry parameter,(N-Z)/A,of the emitting source and(μn-μp)/T=(11.1 1.4)δNN0.21 was *** experimentally extracted(μn-μp)/T and the double ratio temperatures are compared with those from the QSM *** temperatures-,T,and densities,ρ,extracted from the(μn-μp)/T values agreed with those from the double ratio thermometer which used the yield ratios of d,t,h and α *** the two analyses of the differential chemical potential analysis and the initial temperature analysis end up almost identical relation between T and ρ.T=5.25±0.75 MeV is evaluated from the(μn-μp)/Tanalysis,but no density determination was *** the extracted T value,the symmetry energy coefficient Esym =14.6±3.5 MeV is determined for the emitting source of T=5.25±0.75 MeV.

关键词：国际货币基金组织对称能重离子碰撞生产费米能量子统计模型实验观察中等质量碎片

喹硫平联合丙戊酸镁在双相情感障碍抑郁中的应用

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长春中医药大学学报 2024年第7期40卷 791-795页

作者：马亦林孙静齐玉陈志强田军南京医科大学附属脑科医院精神科南京210029 宣城市第四人民医院精神科安徽宣城242099 深圳康宁医院抑郁障碍科广州深圳518020

目的探讨喹硫平联合丙戊酸镁在双相情感障碍抑郁中的应用效果。方法依据随机数字表法将2021年6月-2023年4月宣城市第四人民医院收治的106例双相情感障碍抑郁发作患者,分为Ⅰ组和Ⅱ组,各53例。Ⅰ组给予丙戊酸镁,Ⅱ组在Ⅰ组基础上给予小... 详细信息

目的探讨喹硫平联合丙戊酸镁在双相情感障碍抑郁中的应用效果。方法依据随机数字表法将2021年6月-2023年4月宣城市第四人民医院收治的106例双相情感障碍抑郁发作患者,分为Ⅰ组和Ⅱ组,各53例。Ⅰ组给予丙戊酸镁,Ⅱ组在Ⅰ组基础上给予小剂量富马酸喹硫平,2组均持续治疗8周。比较2组治疗8周后的临床疗效;比较2组治疗前、治疗8周后的生化指标、阳性和阴性症状量表(PANSS)、蒙特利尔认知功能评定量表(MoCA)、贝克拉范森躁狂量表(BRMS)评分、生活质量;研究期间安全性。结果治疗8周后,Ⅱ组总有效率为92.45%,高于Ⅰ组的75.47%;与治疗前比较,治疗8周后,2组血清泌乳素(PRL)、促甲状腺激素(TSH)、胆红素(BIL)水平、MoCA评分、生活质量各个维度评分均升高,且Ⅱ组高于Ⅰ组(P<0.05),2组血清尿酸(UA)水平、BRMS、PANSS评分均降低,且Ⅱ组低于Ⅰ组(P<0.05)。结论喹硫平联合丙戊酸镁可稳定双相情感障碍抑郁发作患者体内激素水平,有效解除氧化应激状态,促进患者生活质量、躁狂症状及精神状态的改善,进而提高临床治疗效果及患者的认知功能,且安全性良好。

关键词：双相情感障碍抑郁丙戊酸镁喹硫平生化指标临床症状

水煤浆添加剂TS-M1对几种煤成浆效果的试验研究

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煤化工 2024年第1期52卷 56-59页

作者：胡兴刚王润宇陈志强中海油天津化工研究设计院有限公司天津300131 天津正达科技有限责任公司天津300308

为了考察复合高效水煤浆添加剂TS-M1在水煤浆制浆中的整体成浆效果,以4种特定的煤种为制浆对象,对煤浆质量分数、黏度、流动性及稳定性等进行了量化分析。试验结果及现场应用情况表明:针对煤气化项目现场的应用条件及4种特定煤种,复合... 详细信息

为了考察复合高效水煤浆添加剂TS-M1在水煤浆制浆中的整体成浆效果,以4种特定的煤种为制浆对象,对煤浆质量分数、黏度、流动性及稳定性等进行了量化分析。试验结果及现场应用情况表明:针对煤气化项目现场的应用条件及4种特定煤种,复合高效水煤浆添加剂TS-M1在常规添加量1‰~4.5‰范围内,可制得最高成浆质量分数分别为64%、63.5%、65%和68%的水煤浆,同时能保持良好的煤浆黏度、流动性及稳定性。

关键词：水煤浆 TS-M1添加剂成浆性粒度级配

职业性尘肺病合并间质性肺炎56例临床特点分析

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中国职业医学 2024年第4期51卷 415-418页

作者：白梅张久红陈志强谢伟民广西职业病防治研究院尘肺病科二区

目的探讨职业性尘肺病（以下简称“尘肺病”）合并间质性肺炎（ILD）的临床特点。方法采用回顾性研究方法，选择56例尘肺病合并ILD患者为研究对象，分析其临床特征。结果 56例患者的临床症状主要为进行性呼吸困难、咳嗽和咳痰，检出... 详细信息

目的探讨职业性尘肺病（以下简称“尘肺病”）合并间质性肺炎（ILD）的临床特点。方法采用回顾性研究方法，选择56例尘肺病合并ILD患者为研究对象，分析其临床特征。结果 56例患者的临床症状主要为进行性呼吸困难、咳嗽和咳痰，检出率分别为76.8%、75.0%和55.4%；低氧血症、呼吸衰竭检出率分别为67.9%和32.1%。35例完成肺功能检查的患者中，限制性、混合性、阻塞性通气功能障碍检出率分别为37.1%、22.9%和17.1%，弥散功能降低检出率为74.3%。胸部高分辨电子计算机断层扫描可见磨玻璃状阴影、网格状阴影、小叶间隔增厚、蜂窝状阴影和牵拉性支气管扩张，检出率分别为57.1%、35.7%、35.7%、23.2%和17.9%；病变多为两肺弥漫性分布，下肺分布多于中上肺。有19例（占33.9%）患者合并结缔组织疾病，包括合并系统性硬化症、类风湿性关节炎、系统性红斑狼疮、多发性肌炎与皮肌炎。结论尘肺病合并ILD患者的临床症状、肺功能与血气分析结果均无特异性，但部分尘肺病患者合并ILD的同时可合并有结缔组织疾病。

关键词：尘肺病间质性肺炎高分辨电子计算机断层扫描结缔组织病

Experimental reconstruction of primary fragments with kinematical focusing method

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Nuclear Science and Techniques 2013年第5期24卷 54-58页

作者： LIN Weiping WADA Roy HUANG Meirong LIU Xingquan ZHAO Minghui chen zhiqiang Institute of Modern Physics Chinese Academy of Science University of Chinese Academy of Sciences

An experimental method was used to evaluate the primary isotope yields of semi-central collisions in the reaction system64Zn+112Sn at 40A *** characteristic nature of the hot nuclear matter at the time of the isotope formation was *** multiplicities of light particles(LPs) associated with intermediate mass fragments(IMFs) were determined experimentally by using a kinematical focusing *** primary isotope distributions,reconstructed by a Monte Carlo method,were compared with those of the AMD-Gemini ***/T=0.11 and asym/T=3.34 were extracted from the reconstructed primary fragments *** are consistent with those of the primary fragments of the AMD simulation.

关键词：运动学主段实验聚焦法同位素分布中等质量碎片蒙特卡罗方法反应体系

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实验辅助“电子技术”课堂教学探索与实践

电气电子教学学报 2024年第3期46卷 211-214页

作者：陈志强王丽安斯光童仁圆李青中国计量大学电工电子教学中心杭州310018

探索了“电子技术”课程JIT(Just In Time,即时)实验辅助课堂教学的课程改革。通过分析教学中存在的问题,优化了教学内容,搭建了灵活、便携的JIT实验平台,设计了JIT实验用例。课堂实践结果表明,JIT实验辅助教学激发了学生的学习热情,提... 详细信息

探索了“电子技术”课程JIT(Just In Time,即时)实验辅助课堂教学的课程改革。通过分析教学中存在的问题,优化了教学内容,搭建了灵活、便携的JIT实验平台,设计了JIT实验用例。课堂实践结果表明,JIT实验辅助教学激发了学生的学习热情,提高了学生的工程实践能力,收到良好的教学效果。

关键词：课堂教学教学改革电子技术

UAV swarm air combat maneuver decision-making method based on multi-agent reinforcement learning and transferring

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Science China(Information Sciences) 2024年第8期67卷 49-66页

作者： zhiqiang ZHENG chen WEI Haibin DUAN State Key Laboratory of Virtual Reality Technology and Systems School of Automation Science and Electrical Engineering Beihang University

During short-range air combat involving unmanned aircraft vehicle(UAV) swarms, UAVs must make accurate maneuver decisions based on information from both enemy and friendly UAVs. This dual requirement of competition and cooperation presents a significant challenge in the field of unmanned air combat. In this paper, a method based on multi-agent reinforcement learning(MARL) is proposed to address this issue. An actor network containing three subnetworks that can handle different types of situational information is designed. Hence, the results from simpler one-on-one scenarios are leveraged to enhance the complex swarm air combat training process. Separate state spaces for local and global information are designed for the actor and critic networks. A detailed reward function is proposed to encourage *** prevent lazy participants in air combat, a reward assignment operation is applied to distribute these dense rewards. Simulation testing and ablation experiments demonstrate that both the transfer operation and reward assignment operation can effectively deal with the swarm air combat scenario, and reflect the effectiveness of the proposed method.

关键词： UAV swarm short-range air combat multi-agent reinforcement learning reward assignment transfer

Two-Sided Empirical Bayes Test for the Exponential Family with Contaminated Data

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Wuhan University Journal of Natural Sciences 2013年第6期18卷 466-470页

作者： chen Jiaqing JIN Qianyu chen zhiqiang LIU Cihua College of Science Wuhan University of Technology College of Mathematics and Statistics Huazhong University of Science and Technology

In this study, the two-sided Empirical Bayes test （EBT） rules for the parameter of continuous one-parameter exponential family with contaminated data （errors in variables） are constructed by a deconvolution kernel method. The asymptotically optimal uniformly over a class of prior distributions and uniform rates of convergence, which depends on two types of the error distribu- tions for the proposed EBT rules, are obtained under suitable con- ditions. Finally, an example about the main results of this paper is given.

关键词： empirical Bayes test asymptotic optimal conver-gence rate contaminated data