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Suppression of matrix metalloproteinase-2 via RNA interference inhibits pancreatic carcinoma cell invasiveness and adhesion

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World Journal of Gastroenterology 2009年第9期15卷 1072-1078页

作者： Ying-Hui zhi Mao-min song Pi-Lin Wang Tie Zhang Zi-Yi Yin Department of General Surgery Affiliated Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

AIM:To investigate the inhibitory effects of RNA interference (RNAi) on expression of matrix metalloproteinase-2 (MMP-2) gene and invasiveness and adhesion of human pancreatic cancer cell line,***:RNAi was performed using the vector (pGPU6)-based small interference RNA (siRNA) plasmid gene silence system to specifically knock down MMP-2 expression in pancreatic cancer cell line,BxPC-3. Four groups of different specific target sequence in coding region of MMP-2 and one non-specific sequence were chosen to construct four experimental siRNA plasmids of pGPU6-1,pGPU6-2,pGPU6-3 and pGPU6-4,and one negative control siRNA plasmid of pGPU6 (-). MMP-2 expression was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation and apoptosis were examined by methyl thiazolyl tetrazolium (MTT) and flow cytometry,respectively. The abilities of adhesion and invasion were detected by cell adhesion assay and cell invasion assay using Transwell ***:The expression of MMP-2 was inhibited and the inhibitory effects of different sequence varied. pGPU6-1 group had the most efficient inhibitory effect,followed by pGPU6-2 and pGPU6-3 *** and adhesion were more significantly reduced in pGPU6-1,pGPU6-2 and pGPU6-3 groups as compared with pGPU6 (-) and blank control groups. However,no difference concerning cell proliferation and apoptosis was observed after transfection between experiment groups and control ***:RNAi against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the pancreatic cancer cell line,BxPC-3,leading to a potent suppression of tumor cell adhesion and invasion without affecting cell proliferation and apoptosis. These findings suggest that the RNAi approach towards MMP-2 may be an effective therapeutic strategy for the clinical management of pancreatic tumor.

关键词： Pancreatic neoplasm Tumor metastasis Matrix metalloproteinase-2 Small interfering RNA Tumor invasiveness

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Direct Z-scheme CdS-CdS Nanorod Arrays Photoanode: Synthesis,Characterization and Photoelectrochemical Performance

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Chinese Journal of Chemical Physics 2019年第6期32卷 715-720,I0003,I0025-I0031页

作者： Yi Fan zhi-min song Jing-jing Dong zhi-yu Wang Yue Yang Xiao-di Zhu song Sun Chen Gao Jun Bao National Synchrotron Radiation Laboratory Collaborative Innovation Center of Chemistry for Energy MaterialsUniversity of Science and Technology of ChinaHefei 230029China School of Chemistry and Chemical Engineering Anhui UniversityHefei 230601China Beijing Advanced Sciences and Innovation Center Chinese Academy of SciencesBeijing 101407China

Direct Z-scheme CdO-CdS 1-dimensional nanorod arrays were constructed through a facile and simple hydrothermal process. The structure, morphology, photoelectrochemical properties and H2 evolution activity of this catalyst were investigated systematically. The morphology of the obtained nanorod is a regular hexagonal prism with 100-200 nm in diameter. The calcination temperature and time were optimized carefully to achieve the highest photoelectrochemical performance. The as-fabricated hybrid system achieved a photocurrent density up to 6.5 mA/cm2 and H2 evolution rate of 240 μmol·cm-2·h-1 at 0 V vs. Ag/AgCl, which is about 2-fold higher than that of the bare CdS nanorod arrays. The PEC performance exceeds those previously reported similar systems. A direct Z-scheme photocatalytic mechanism was proposed based on the structure and photoelectrochemical performance characterization results, which can well explain the high separation efficiency of photoinduced carriers and the excellent redox ability.

关键词： Direct Z-scheme CdO-CdS One-Dimensional nanorod arrays PEC performance H2 evolution rate

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Unusual Insertion Reaction of CO2 and Tantalum(Ⅴ)Metallaheterocyclic Compound

Unusual Insertion Reaction of CO2 and Tantalum(Ⅴ)Metallahet...

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中国化学会2013年中西部地区无机化学化工学术研讨会

作者： Li Wang zhi-min song Hu Cai Department of chemistry Nanchang University

The insertion reactions of CO2 with transition metal amide or imide complexes have been well ***,studies of transition metal metallaheterocyclic compounds and CO have received little *** interest was especially focused on the insertion reaction of CO and Tantalum(Ⅴ) metallaheterocyclic *** our studies,the reaction of CO and complex 1 was found to give an unusual product of *** this reaction,three molecules of CO were likely inserted

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全膝人工关节置换术中髌骨置换对股四头肌肌力的影响

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中华医学杂志 2008年第12期88卷 809-811页

作者：刘帆吕厚山闵志松北京大学人民医院关节病诊疗研究中心 100044

目的探讨髌骨置换后髌骨轨迹、髌骨厚度等对股四头肌力的影响，为指导人工膝关节置换术髌骨关节置换方法及术后膝关节功能的恢复提供理论依据。方法收集退变性骨性关节炎行膝关节置换术患者100例134个膝关节，由同一组医生进行同种关节... 详细信息

目的探讨髌骨置换后髌骨轨迹、髌骨厚度等对股四头肌力的影响，为指导人工膝关节置换术髌骨关节置换方法及术后膝关节功能的恢复提供理论依据。方法收集退变性骨性关节炎行膝关节置换术患者100例134个膝关节，由同一组医生进行同种关节假体关节置换，根据患者术后髌股关节情况分为3组，采用CYBEX-6000型等速肌力测试系统测试股四头肌肌力，采用伸展滞缺角度评价伸肌肌力。结果髌骨轨迹不良以及髌骨假体安装位置不当造成股四头肌肌力和耐力明显下降，髌骨轨迹不良组（61±24）、髌骨假体位置不良组（56±33）与髌股关节正常组（86±22）间差异有统计学意义（P〈0．01）。结论髌前疼痛等临床症状主要与髌骨轨迹不良相关，而单纯的股四头肌无力主要与置换后的髌骨厚度有关；研究髌股关节生物力学与髌骨置换技术，有利于提高全膝置换手术疗效。

关键词：人工关节髌骨肌收缩

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Complete dissociation of respiratory burst from extracellular trap formation in the human leukemia cell HL-60

Complete dissociation of respiratory burst from extracellula...

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第九届全国钙信号和细胞功能研讨会

作者： zhi min song,Zong Jie Cui Institute of Cell Biology,Beijing Normal University,Beijing 100875,China

Neutrophils kill and clean up pathogens by both respiratory burst and the formation of extracellular *** human leukemic HL-60 cells show typical neutrophil properties after *** present work investigates the capability of HL-60 cells to produce respiratory burst and to form extracellular traps at difierent stages of difrerentiation and compare these properties with those of the freshly isolated rat *** was found that PMA stimulation of rat neutrophils triggered respiratory burst and extracellular trap *** identified MPO,NE and

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Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

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Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo min song Lulu An Xiao Huang Qian Zhang Chenhao Zhang Tao Shen Shuang Wang Deli Wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Synergistic effect Intermetallics Single atomic sites Oxygen reduction reaction Hydrogen evolution reaction

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黄河下游流域的文化界说——兼论华北平原在黄河文化中的历史地位

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河北学刊 2025年第1期45卷 98-110页

作者：王志民宋宁山东师范大学齐鲁文化研究院

黄河下游流域与文化的界定,是黄河文化研究中的一个重大问题。论者将黄淮海平原的广阔区域都作为黄河下游流域的传统看法是不恰当的。淮河作为一条大河,自有其独立的流域范围,“夺淮入海”时期,黄河夺占淮河支流和主河道,主要系人为因... 详细信息

黄河下游流域与文化的界定,是黄河文化研究中的一个重大问题。论者将黄淮海平原的广阔区域都作为黄河下游流域的传统看法是不恰当的。淮河作为一条大河,自有其独立的流域范围,“夺淮入海”时期,黄河夺占淮河支流和主河道,主要系人为因素改道和维持所致,且多为河道游移、水系混乱的“地上河”,并未形成自己的“集水区”;在文化上,淮河文化是长江文化的亚文化,在春秋战国时期是长江楚文化的重要组成部分。故无论从流域还是文化上看,淮河流域都不能作为黄河下游流域看待。泰沂山西延伏脉以北的华北平原,是黄河天然形成的入海之地;在秦汉以前,是中华文明起源、三代文明奠基的重要区域之一,是春秋战国时代的文化重心,是实现秦汉“大一统”的经学繁荣基地和思想库。该地区在中华文明起源、形成及中华民族共同体奠基的重要历史时期作出了突出贡献,是奠定黄河文化为中华民族“根”与“魂”的核心区之一。华北平原及其文化就是黄河下游的流域与文化。

关键词：黄河下游文化界说华北平原黄河文化

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移动IPv6通信与防火墙冲突问题研究

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计算机工程与设计 2007年第11期28卷 2575-2576,2695页

作者：王峰闵志松王清贤解放军信息工程大学河南郑州450002

IPv6协议取代IPv4已经成为趋势,IPv6对移动IP的具有更强的支持,但是现阶段防火墙存在无法正确处理移动IPv6数据包的问题。在分析移动IPv6的通信机制以及现阶段包过滤防火墙特性的基础上,针对移动节点在通信过程中位置变换的情况,分类探... 详细信息

IPv6协议取代IPv4已经成为趋势,IPv6对移动IP的具有更强的支持,但是现阶段防火墙存在无法正确处理移动IPv6数据包的问题。在分析移动IPv6的通信机制以及现阶段包过滤防火墙特性的基础上,针对移动节点在通信过程中位置变换的情况,分类探讨了移动IPv6的通信机制与现阶段防火墙过滤规则的各种冲突现象,总结了冲突的原因并提出了解决方案。

关键词：移动IPv6 防火墙包过滤返回路由可达性测试绑定更新

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红枣中天然苯甲酸的溯源分析及本底调查

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食品科技 2017年第9期42卷 290-293页

作者：巩志国苏敏宋姣伍新宇新疆出入境检验检疫局乌鲁木齐830063 新疆农业科学院园艺作物研究所乌鲁木齐830043

苯甲酸(苯甲酸盐)天然存在于多种植物(浆果、香料和核果)及发酵食品中,植物中的苯甲酸及其衍生物是植物生长过程中的次级代谢产物。红枣中就含有天然苯甲酸。使用3种不同品牌的色谱柱,在相同的色谱条件下,红枣样品均呈现出与苯甲酸标准... 详细信息

苯甲酸(苯甲酸盐)天然存在于多种植物(浆果、香料和核果)及发酵食品中,植物中的苯甲酸及其衍生物是植物生长过程中的次级代谢产物。红枣中就含有天然苯甲酸。使用3种不同品牌的色谱柱,在相同的色谱条件下,红枣样品均呈现出与苯甲酸标准品相同的保留时间和紫外吸收光谱图,证实了红枣中天然苯甲酸的存在。通过对新疆主产区主栽的红枣品种生长过程中的苯甲酸含量进行分析,表明天然苯甲酸的生成时期为红枣果实生育期,并随着果实成熟而逐渐增加。通过对全国3个主产区共122批干制红枣样品进行检测,初步明确了红枣中天然苯甲酸的本底值范围,含量普遍小于0.15 g/kg。为制订或修订红枣中苯甲酸含量相关标准提供了重要依据。

关键词：红枣天然苯甲酸溯源分析本底值

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Extramedullary plasmacytoma with the uvula as first affected site:A case report

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World Journal of Clinical Oncology 2025年第1期16卷 53-58页

作者： Jing Yang Hui Peng Sheng-Ke Tu min Li Kui song Department of Hematology The First Affiliated Hospital of Jishou UniversityJishou 416000Hunan ProvinceChina Department of Pharmacy The First Affiliated Hospital of Jishou UniversityJishou 416000Hunan ProvinceChina

BACKGROUND Extramedullary plasmacytoma(EMP)represents one of the rarer forms of plasma cell malignancies,capable of impacting a variety of tissues and organs throughout the *** majority of EMP cases are predominantly found in the head and neck region,especially within the laryngopharynx,as well as in the gastrointestinal *** there have been documented instances of oropharyngeal involvement in EMP cases in the academic literature,it is important to note that EMP specifically affecting the uvula is exceedingly ***,it is noteworthy that over 60% of epithelial carcinomas in the upper respiratory tract and oropharynx tend to metastasize to the cervical lymph nodes,indicating a propensity for regional spread in these types of *** this context,we present a rare case of extramedullary plasmacytoma where the uvula served as the initially affected *** case emphasizes the need for heightened awareness among clinicians regarding such unusual comorbidities,as early recognition and diagnosis can significantly influence patient management and treatment *** addition,a review of the relevant literature is included to further educate and inform healthcare professionals about this rare presentation,ultimately aiming to enhance clinical understanding and improve patient care in similar *** SUMMARY A 51-year-old man was admitted to our hospital because of a slowly enlarging neck mass.A physical examination revealed a palpable left lymph node,and magnetic resonance imaging(MRI)of the oropharynx and the neck showed a soft tissue mass in the oropharynx and enlargement of multiple lymph nodes in the *** soft tissue mass was diagnosed as plasmacytoma by immunohistochemical *** immunoglobulins and bone marrow biopsy showed normal ***,we diagnosed that as EMP of the *** four cycles of adjuvant chemotherapy dominated by bortezomib,MRI reexamination showed a significant reduction of the mass

关键词： Extramedullary plasmacytoma Plasmacytoma Uvula Multiple myeloma Case report

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