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Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第11期20卷 891-900页

作者： Zhang-qing SONG zhi-jun liao Ye-feng HU Zheng MA Andreas BECHTHOLD Xiao-ping YU Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine College of Life SciencesChina Jiliang UniversityHangzhou 310018China Institute for Pharmaceutical Sciences Pharmaceutical Biology and BiotechnologyUniversity of Freiburg79104 FreiburgGermany

An efficient genetic transformation system and suitable promoters are essential prerequisites for gene expression studies and genetic engineering in *** this study,firstly,a genetic transformation system based on intergeneric conjugation was developed in Streptomyces rimosus M527,a bacterial strain which exhibits strong antagonistic activity against a broad range of plant-pathogenic *** experimental parameters involved in this procedure were optimized,including the conjugative media,ratio of donor to recipient,heat shock temperature,and incubation time of mixed *** the optimal conditions,a maximal conjugation frequency of 3.05^10-5 per recipie nt was obtai *** ntly,based on the above developed and optimized tran sformati on system,the synthetic promoters SPL-21 and SPL-57,a native promoter potrB,and a constitutive promoter permE commonly used for gene expression in streptomycetes were selected and their activity was analyzed using gusA as a reporter gene in *** *** the four tested promoters,SPL-21 exhibited the strongest expression activity and gave rise to a 2.2-fold increase in p-glucuronidase(GUS)activity compared with the control promoter *** SPL-57 showed activity comparable to that of *** potrB,which showed the lowest activity,showed a 50%decrease in GUS activity compared with the control *** transformation system developed in this study and the tested promotors provide a basis for the further modification of *** M527.

关键词： Streptomyces rimosus M527 Intergeneric conjugation Promoter β-Glucuronidase(GUS)

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NFT Security Matrix:Towards Modeling NFT Ecosystem Threat

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Computer Modeling in Engineering & Sciences 2024年第6期139卷 3255-3285页

作者： Peng liao Chaoge Liu Jie Yin zhi Wang Xiang Cui Key Laboratory of Trustworthy Distributed Computing and Service Beijing University of Posts and TelecommunicationsBeijing100876China Zhongguancun Laboratory Beijing100094China Institute of Information Engineering Chinese Academy of SciencesBeijing100085China

Digital assets have boomed over the past few years with the emergence of Non-fungible Tokens(NFTs).To be specific,the total trading volume of digital assets reached an astounding$55.5 billion in ***,numerous security concerns have been raised by the rapid expansion of the NFT *** holders are exposed to a plethora of scams and traps,putting their digital assets at risk of being ***,academic research on NFT security is scarce,and the security issues have aroused rare *** this study,the NFT ecological process is comprehensively *** process falls into five different stages encompassing the entire lifecycle of ***,the security issues regarding the respective stage are elaborated and analyzed in depth.A matrix model is proposed as a novel contribution to the categorization of NFT security *** data are collected from social networks,the Ethereum blockchain,and NFT markets to substantiate our claims regarding the severity of security concerns in the NFT *** this comprehensive dataset,nine key NFT security issues are identified from the matrix model and then subjected to qualitative and quantitative *** study aims to shed light on the severity of NFT ecosystem security *** findings stress the need for increased attention and proactive measures to safeguard the NFT ecosystem.

关键词： Non-fungible token blockchain cyber security

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One-shot synthesis of heavy-atom-modified carbazole-fused multi-resonance thermally activated delayed fluorescence materials

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Science China Materials 2024年第9期67卷 2789-2795页

作者： Jia-jun Hu Jia-Qi Liang zhi-Ping Yan Hua-Xiu Ni Xiang-Ji liao You-Xuan Zheng State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic MaterialsSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou450001China

Efficient multi-resonance thermally activated delayed fluorescence(MR-TADF)materials hold significant potential for applications in organic light-emitting diodes(OLEDs)and ultra-high-definition ***,the stringent synthesis conditions and low yields typically associated with these materials pose substantial challenges for their practical *** this study,we introduce an innovative strategy that involves peripheral modification with sulfur and selenium atoms for two materials,CFDBNS and *** approach enables a directed one-shot borylation process,achieving synthesis yields of 66%and 25%,respectively,while also enhancing reverse intersystem crossing *** emitters exhibit ultra-narrowband sky-blue emissions centered around 474 nm,with full width at half maximum(FWHM)values as narrow as 19 nm in dilute toluene solutions,along with high photoluminescence quantum yields of 98%and 99%in doped films,*** OLEDs based on CFDBNS and CFDBNSe display sky-blue emissions with peaks at 476 and 477 nm and exceptionally slender FWHM values of 23 ***,the devices demonstrate remarkable performances,achieving maximum external quantum efficiencies of 24.1%and 27.2%.This work presents a novel and straightforward approach for the incorporation of heavy atoms,facilitating the rapid construction of efficient MR-TADF materials for OLEDs.

关键词： one-shot synthesis multi-resonance thermally activated delayed fluorescence carbazole-fused dual-boron embedded framework ultra-narrowband emission organic light-emitting diode

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Triphenylphosphine-Assisted Exsolution Engineering on Ruddlesden-Popper Perovskites for Promoting Oxygen Evolution

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Energy & Environmental Materials 2024年第4期7卷 433-441页

作者： Juan Bai Jing Shang jun Mei Dongchen Qi Ting liao Ziqi Sun School of Chemistry and Physics Queensland University of Technology Centre for Materials Science Queensland University of Technology School of Materials Science and Engineering Shaanxi University of Science and Technology School of Mechanical Medical and Process Engineering Queensland University of Technology

Metal exsolution engineering has been regarded as a promising strategy for activating intrinsically inert perovskite oxide catalysts toward efficient oxygen evolution *** metal exsolution processes on perovskites are often achieved by using the reducing hydrogen gas; however,this is not effective for the relatively stable phase,such as Ruddlesden-Popper perovskite oxides.

关键词： exsolution oxygen evolution perovskite reconstruction Ruddlesden–Popper

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Controllable image generation based on causal representation learning

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Frontiers of Information Technology & Electronic Engineering 2024年第1期25卷 135-148页

作者： Shanshan HUANG Yuanhao WANG zhili GONG jun liao Shu WANG Li LIU School of Big Data and Software Engineering Chongqing UniversityChongqing 401331China School of Materials and Energy Southwest UniversityChongqing 400715China

Artificial intelligence generated content(AIGC)has emerged as an indispensable tool for producing large-scale content in various forms,such as images,thanks to the significant role that AI plays in imitation and ***,interpretability and controllability remain *** AI methods often face challenges in producing images that are both flexible and controllable while considering causal relationships within the *** address this issue,we have developed a novel method for causal controllable image generation(CCIG)that combines causal representation learning with bi-directional generative adversarial networks(GANs).This approach enables humans to control image attributes while considering the rationality and interpretability of the generated images and also allows for the generation of counterfactual *** key of our approach,CCIG,lies in the use of a causal structure learning module to learn the causal relationships between image attributes and joint optimization with the encoder,generator,and joint discriminator in the image generation *** doing so,we can learn causal representations in image’s latent space and use causal intervention operations to control image *** conduct extensive experiments on a real-world dataset,*** experimental results illustrate the effectiveness of CCIG.

关键词： Image generation Controllable image editing Causal structure learning Causal representation learning

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Preparation of three-dimensional nanoporous copper foil with high specific surface area

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Rare Metals 2024年第7期43卷 3430-3437页

作者： Ning Song Li-Juan Wang jun-Feng Wang Juan liao Jian Huang Peng-Kang Du Yun-zhi Tang Yu-Hui Tan Xiao-Wei Fan Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry School of Chemistry and Chemical EngineeringJiangxi University of Science and TechnologyGanzhou 341000China

The porous copper foil material has the advantages of ultralight and ultra-thin,and can prevent dangers such as thermal runaway and battery fire,which could be regarded as a prospective material for lithium battery **... 详细信息

关键词： copper porous dimensional

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FPR1 Antagonist (BOC-MLF) Inhibits Amniotic Epithelial-mesenchymal Transition

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Current Medical Science 2024年第1期44卷 187-194页

作者： Xiao-mei HUANG E liao jun-qun liao Ya-ling LIU Yong SHAO Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China Medical Laboratory Science The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Yubei Maternity and Child Healthcare HospitalChongqing 400042China

Objective:Premature rupture of membranes(PROM)is a common pregnancy disorder that is closely associated with structural weakening of fetal *** have found that formyl peptide receptor 1(FPR1)activates inflammatory pathways and amniotic epithelial-mesenchymal transition(EMT),stimulates collagen degradation,and leads to membrane weakening and membrane *** purpose of this study was to investigate the anti-inflammatory and EMT inhibitory effects of FPR1 antagonist(BOC-MLF)to provide a basis for clinical prevention of ***:The relationship between PROM,FPR1,and EMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting,PCR,Masson staining,and ELISA ***(LPS)was used to establish a fetal membrane inflammation model in pregnant rats,and BOC-MLF was used to treat the LPS rat *** detected interleukin(IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was *** used electron microscopy to analyze the structure and collagen expression of rat fetal ***:Western blotting,PCR and Masson staining indicated that the expression of FPR1 was significantly increased,the expression of collagen was decreased,and EMT appeared in *** rat model indicated that LPS caused the collapse of fetal membrane epithelial cells,increased intercellular gaps,and decreased ***-MLF promoted an increase in fetal membrane collagen,inhibited EMT,and reduced the weakening of fetal ***:The expression of FPR1 in the fetal membrane of PROM was significantly increased,and EMT of the amniotic membrane was ***-MLF can treat inflammation and inhibit amniotic EMT.

关键词： formyl peptide receptor 1 BOC-MLF epithelial-mesenchymal transition premature rupture of membranes

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Re-evaluating the nano-sized inorganic protective layer on Cu current collector for anode free lithium metal batteries

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Chinese Chemical Letters 2024年第7期35卷 452-455页

作者： Shuo Zhang Haitao liao zhi-Qun Liu Chong Yan Jia-Qi Huang School of Materials Science&Engineering Beijing Institute of TechnologyBeijing 100081China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Wuxi Yiwen Microelectronics Technology Co. Ltd.Wuxi 214000China Shanxi Research Institute for Clean Energy Tsinghua UniversityTaiyuan 030032China

Anode free lithium metal batteries(AF-LMBs)have conspicuous advantages both in energy density and the compatibility of battery manufacturing ***,the limited cycle life of AF-LMBs is a crucial factor hindering its practical *** or nitride artificial inorganic solid electrolyte interphase(SEI)has been found as an effective method to prolong the lifespan of ***,by investigating the impact of nano-sized inorganic gradient layers(LiF or Li3N)on initial Li deposition behavior,we notice that the Li^(+) diffusion barrier and the deposition morphology are highly depended on the thickness of inorganic *** protective layers cause larger overpotential as well as more aggregated Li^(+) *** study reveals that the ideal SEI should be synthesized thin and uniformly enough and uncontrollable artificial SEI can cause damage to the lifespan of AF-LMBs.

关键词： Anode free lithium metal battery Solid electrolyte interphase Nano-sized protective layer Lithium fluoride Li deposition behavior

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Teffichthys wui sp. nov., a new perleidid fish from the Early Triassic of Jiangsu and Anhui, China

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古脊椎动物学报（中英文） 2024年第3期62卷 165-185页

作者： XU Guang-Hui YUAN zhi-Wei REN Yi liao jun-Ling ZHAO Li-jun SONG Hai-jun Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044 State Key Laboratory of Biogeology and Environmental Geology School of Earth SciencesChina University of GeosciencesWuhan 430074 University of Chinese Academy of Sciences Beijing 100049 College of Economics and Trade Minzu Normal University of Xingyi XingyiGuizhou 562400 Zhejiang Museum of Natural History Hangzhou 310014

Perleididae is a group of stem neopterygian fishes known only from the ***,we report the discovery of a new perleidid,Teffichthys wui ***.,based on six well-preserved specimens from the late Smithian(Olenekian,Early Triassic)marine deposits of Jurong,Jiangsu and Chaohu,Anhui,*** new discovery documents the third and youngest species of Teffichthys,which is slightly younger than the Dienerian(Induan)*** from Guizhou and the early Smithian *** from *** new species shows diagnostic features of Teffichthys(presence of a spiracular,38-41 lateral line scales,and no more than three epaxial rays in the caudal fin)but differs from *** and *** in some autapomorphies(e.g.,a horizontal opercle/subopercle contact and smooth scales with a nearly straight posterior margin).The diagnostic features for the genus Teffichthys and the family Perleididae are emended based on detailed comparisons of the new taxon with other *** phylogenetic relationships of perleidids with other stem neopterygians are discussed using a cladistic approach,and the results provide new insights into the phylogeny and classification of main stem neopterygian clades.

关键词： Jiangsu Anhui Early Triassic Perleidiformes osteology phylogeny

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Characteristics and quality of clinical practice guidelines for diabetic kidney disease:a systematic review

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Journal of Traditional Chinese Medicine 2024年第3期44卷 609-619页

作者： WANG Yuhuang ZHANG Le ZHANG Zhengshan YAO zhi LI Xiyao SUN Luying liao Xing Department of Nephropathy and Endocrine Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100010China Centre for Evidence-Based Chinese Medicine Institute of Basic Research in Clinical MedicineChina Academy of Chinese Medical SciencesBeijing 100010China Department of Endocrine One Zone Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China

OBJECTIVE:To assess the quality of Clinical practice guidelines(CPGs)in the context of diabetic kidney disease(DKD)and determine whether any factors affect the ***:We searched eight databases along with five international and national organizations to develop or archive guidelines from their inception to July 2023,with an additional search of *** the authoritative organizations related to *** and consensus statements created using direct differential diagnosis or therapy for DKD were included without language *** quality was evaluated by four reviewers using the Appraisal of Guidelines for Research and EvaluationⅡ(AGREEⅡ)*** with the item and domain scores,the guideline was also allocated an overall quality score,which ranged from 1(lowest possible quality)to 7(highest possible quality).Moreover,an overall recommendation for use was also assigned(“recommended”,“recommended with modifications”or“not recommended”).RESULTS:A total of 16 CPGs were included,of which 14 were from Asia and the remaining two from *** two CPGs were updated in the third *** CPGs were recommended for use because their primary domains scored in the medium or high ***,five CPGs were recommended with modifications as the stakeholder involvement,applicability,and editorial independence domains were evaluated as low *** all domains,the lowest average score was for rigour of development(33%),followed by application(36%),and stakeholder involvement(51%).The highest average score was for scope and purpose(79%),followed by clarity of presentation(75%).None of the CPGs considered the patient's viewpoint,and six of 16 CPGs did not use any grading system to translate the evidence into ***,only three of 16 CPGs shared search strategy,and eight of 16 CPGs did not declare a funding ***:According to the AGREE II evaluation,more than one in four CPGs for DKD had poo

关键词： diabetic nephropathies practice guideline appraisal of guidelines for research and evaluation II quality assessment systematic review

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