检索结果-南通市图书馆

Journal of Iron and Steel Research International 2014年第2期21卷 159-165页

作者： De-wei LI zhi-jian su Katsukiyo MARUKAWA Ji-cheng HE Key Laboratory of Electromagnetic Processing of Materials of Ministry of Education Northeastern University

A new process for swirling flow generation in the submerged entry nozzle （SEN） in continuous casting process of steel was proposed. A rotating electromagnetic field was set up around the SEN to induce swirling flow by Lorentz force. The flow and temperature fields in the SEN and round billet mold with electromagnetic swirling were numerically simulated and then verified by the electromagnetic swirling model experiment of low melting point alloy. The effects of divergent angle of the SEN on the flow and temperature fields in mold with electromagnetic swirling were investigated. The electromagnetic swirling flow generator （EMSFG） could effectively induce swirling flow of molten steel in the SEN, which consequently improved greatly the flow and temperature fields in the mold. Below the nozzle outlet in mold, with the increase of divergent angle, the stream of bulk flow diverged more widely, the high temperature zone shifted up, and the temperature field became more uniform. Above the nozzle outlet in mold, with 350 A electromagnetic swirling, when the divergent angle of the SEN increased, the upward flow velocity and the meniscus temperature first increased and then decreased. With a divergent angle of 60~, the upward flow velocity and meniscus temperature reaced the largest value.

关键词： continuous casting process swirling flow flow field divergent angle round billet

Abnormal mold level fluctuation during slab casting of peritectic steels

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2020年第2期27卷 160-168页

作者： Zhong-kuai jiang zhi-jian su Cheng-qian Xu Jin Chen Ji-cheng He Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education) Northeastern UniversityShenyang 110004LiaoningChina Shanghai Meishan Iron and Steel Co. Ltd.Nanjing 210039JiangsuChina School of Metallurgy Northeastern UniversityShenyang 110004LiaoningChina

Abnormal mold level fluctuation is frequently observed in high-speed casting,especially for peritectic steels,which has been shown to severely deteriorate product *** an online electromagnetic sensor and an ibaAnalyzer are used to analyze the frequency and amplitude of massive mold level fluctuation data from the slab casting process of different steel grades using different casting speeds,slab widths and physical parameters of mold *** results show that in the slab continuous casting process of peritectic steels,the main frequency and equivalent amplitude of abnormal mold level fluctuations first increase and then decrease with increasing the carbon *** the production of various grades of steel,the main frequency of abnormal mold level fluctuation and the equivalent amplitude increase with increasing the casting speed,while the main frequency of abnormal mold level fluctuation changes little with increasing the casting *** main frequency of abnormal mold level fluctuation is positively related to the surface tension of the mold *** on these results,a new mechanism has been *** is shown that the abnormal mold level fluctuation is mainly induced by shell bulging while moving through the rollers,and resonance occurs when the frequency approaches the natural frequency of the molten steel in mold,which results in a large amplitude of the abnormal mold level *** on this mechanism,a new equation for the resonance frequency of molten steel is *** improved method based on this mechanism has proved to be effective in controlling abnormal mold fluctuations.

关键词： Slab continuous casting Abnormal mold level fluctuation Resonance frequency Peritectic steel surface tension

Generation of Leydig-like cells:approaches,characterization,and challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2022年第4期24卷 335-344页

作者： Zhao-Hui Li Jun-Dong Lu Shi-Jun Li Hao-Lin Chen zhi-jian su Guangdong Provincial Key Laboratory of Bioengineering Medicine Department of Cell BiologyJinan UniversityGuangzhou 510632China lnstitute of Life Sciences Wenzhou UniversityWenzhou 325035China Department of Gynecology and Obstetrics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027China National Engineering Research Center of Genetic Medicine Jinan UniversityGuangzhou 510632China

Testosterone production by Leydig cells(LCs)plays a crucial role in male *** functional degeneration of LCs can cause testosterone deficiency,ultimately resulting in primary male *** of exogenous LCs with the ability to produce testosterone in response to the regulation of the hypothalamus-pituitary-gonad axis could be a promising alternative option to treat male primary *** studies have shown that it is possible to generate Leydig-like cells from stem cells by various *** addition,somatic cells,such as embryonic or adult fibroblasts,have also been successfully reprogrammed into Leydig-like *** this review,we summarized the recent advances in the generation of Leydig-like cells,with an emphasis on comparing the effectiveness and safety of different protocols used and the cells *** further analyzing the characteristics of Leydig-like cells generated from fibroblasts based on small signaling molecules and regulatory factors,we found that although the cells may produce testosterone,they are significantly different from real *** future in vivo applications,it is important that the steroidogenic cells generated be evaluated not only for their steroidogenic functions but also for their overall cell metabolic state by proteomics or transcriptomic tools.

关键词： fibroblasts hypogonadism Leydig-like cells reprogramming stem cells

Site-directed Chemical Modification of Recombinant Human aFGF Mutant with Polyethylene Glycol

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2005年第8期16卷 1013-1016页

作者： Qing ZHENG zhi Feng HUANG Xiao Ping WU zhi jian su Hua XU Wen ZHAO Ya Dong HUANG Xiao Kun LI College of Pharmacy Jinan University Guangzhou 510632 Biopharmaceutical R＆D Center of Jinan University Guangzhou 510630

A new product PEGylated rhaFGF was obtained by site-directed chemical *** compared with unmodified rhaFGF, PEGylated rhaFGF showed comparable bioactivity and superior stability at 37℃ in mouse serum and the stronger resistant potency to trypsin. This was accompanied by a substantial decreasing ***-specific PEGylation of rhaFGF may increase its therapeutic potency in humans.

关键词： rhaFGF chemical modification PEGylated rhaFGE

Comparison of main lactobacillus species between healthy women and women with bacterial vaginosis

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2009年第22期122卷 2748-2751页

作者： YAN Dong-hui LU zhi su jian-rong The Center of Clinical Laboratory Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Background The normal microbial flora of the vagina plays an important role in preventing genital and urinary tract infections in women. Thus an accurate understanding of the composition and ecology of the ecosystem is important to understanding the etiology of these diseases. This study aimed to compare the characteristics of main lactobacillus species between healthy women and women with bacterial vaginosis （BV） by quantitative culture and PCR methods. Main lactobacillus species include L. crispatus , L. gasseri, L. jensenii and L. iners.Methods A total of 150 Women attending Gynecology Outpatient Clinic of Beijing Friendship Hospital, were diagnosed as having BV because three or more of the following criteria were met （standard of Amsel＇s composite criteria）： homogeneous discharge, elevated vaginal pH （pH 〉4.5）, production of amines, and presence of ＂clue＂ cells. Those with less than three of the criteria were considered as healthy. Simultaneously, smears were made of vaginal fluid and Gram stained, then were assessed qualitatively as normal （grade Ⅰ）, intermediate （grade Ⅱ）, or consistent with BV （grade Ⅲ）. Gardnerella vaginalis were identified by using Vitek 2 Compact and PCR methods. Lactobacillus species were identified by PCR methods. Gardnerella vaginalis and lactobacilli colony counts were determined by calculating the most number of colonies of each species in the appropriate plates （colonies between 10 and 300）, corrected by the dilution of the sample in the plates, and multiplied by 10 （to account for plating 100 μl）, in order to get colony forming units per milliliter of vaginal secretion.Results BV was diagnosed in 31% （46/150） patients using the composite criteria, the remainder being regarded as healthy. The majority of patients with BV had a smear assessed as grade Ⅲ （91%, 42/46） and minority of them had a smear assessed as grade Ⅱ（9%, 4/46）. The majority of healthy women had a smear assessed as grade Ⅰ （64%, 67/104）. Smears assessed as grade Ⅱ

关键词： bacterial vaginosis vaginal smears lactobacillus Gardnerella vaginalis

Alterations of gene profiles in Leydig-cell-regenerating adult rat testis after ethane dimethane su Ifonate-treatment

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2015年第2期17卷 253-260,I0009页

作者： Yu-Fei Zhang Kai-Ming Yuan Yong Liang Yan-Hui Chu Qing-Quan Lian Yu-Fei Ge Wei Zhen Chantal M Sottas zhi-jian su Ren-Shan Ge Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy Mudanjiang Medical University Mudanjiang 157011 China The 2nd Affiliated Hospital Wenzhou Medical College Wenzhou 325000 China The Affiliated Taizhou Municipal Hospital Taizhou University Taizhou 318000 China The Population Council New York 10065 USA Department of Pathology Molecular Pathology Laboratory Montefiore Medical Centre Albert Einstein College of Medicine Bronx NY 10467 USA College of Life Science and Technology Jinan University Guangzhou 510632 China

成年睾丸间质细胞（ALC）是睾丸中产生雄激素的唯一内分泌细胞，只占有睾丸细胞的1％-5％。大鼠睾丸中的睾丸间质细胞被EDs去除后能够再生。本研究中，我们主要研究了大鼠睾丸经EDS处理后睾丸间质细胞再生及基因的变化图谱。EDS处理后7... 详细信息

成年睾丸间质细胞（ALC）是睾丸中产生雄激素的唯一内分泌细胞，只占有睾丸细胞的1％-5％。大鼠睾丸中的睾丸间质细胞被EDs去除后能够再生。本研究中，我们主要研究了大鼠睾丸经EDS处理后睾丸间质细胞再生及基因的变化图谱。EDS处理后7、21、35、90天后分析血清中睾酮、睾丸基因图谱和一些类固醇生成相关的蛋白变化情况。EDS处理后第7天睾丸中睾酮水平几乎检测不到，然后开始恢复。处理后7天，81个基因下调2倍或者更多，其中48个基因检测不到，包括类固醇合成通路中的蛋白Star、Scarbl、Cypllal、Cypl7al、Hsd3bl、CyplblICyp2al。这些基因在21天开始表达增多，到90天已经完全恢复到正常水平。89个基因上调2倍或者更高，nPkib。这些上调的基因到90天恢复正常；其中Cyp2al直到35天才开始恢复，表明这个基因只在成年睾丸间质细胞中表达，不在前体细胞中表达。qPCR，Westernblot、半定量免疫组织染色检测了随机抽取的基因和蛋白确认其变化情况。

关键词： EDS 基因分析睾丸间质细胞类固醇生成

鲍曼不动杆菌外膜蛋白34的表达纯化及其生物活性分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物技术通报 2017年第7期33卷 185-194页

作者：安志远苏建荣首都医科大学附属北京友谊医院临床检验中心北京100050

通过基因克隆方法制备鲍曼不动杆菌ATCC 19606(Acinetobacter baumannii ATCC 19606)外膜蛋白34(Outer membrance protein 34,Omp34),并分析其生物结构及对HEK293细胞的凋亡作用。采用PCR方法扩增鲍曼不动杆菌ATCC19606Omp34基因,并将其克隆到表达载体p ET28a(+)以构建重组质粒pET28a(+)-Omp34,转化大肠杆菌BL21,IPTG诱导蛋白表达并用镍柱亲和层析纯化蛋白,使用L-精氨酸和还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)可使Omp34蛋白正确复性,生物信息预测Omp34生物结构,利用圆二色谱(Circular dichroism CD)技术测定Omp34的二级结构,通过流式细胞术和Western blot检测Omp34对HEK293细胞的凋亡作用。成功构建表达载体p ET28a(+)-Omp34,并在原核表达体系中高效表达,通过复性得到可溶蛋白。生物信息预测Omp34为β桶状蛋白,圆二色谱证实Omp34结构为β折叠结构,其中α-螺旋含量为12%、β-折叠为39%、β-转角为22%、无规则卷曲为27%。复性的Omp34可使HEK293细胞发生凋亡。通过分子克隆技术成功获得了鲍曼不动杆菌外膜蛋白34,并解析出其二级结构,证实其可以导致HEK293细胞凋亡,这为将来进一步研究鲍曼不动杆菌外膜蛋白34的生物学功能及其耐药机制奠定了基础。

关键词：鲍曼不动杆菌外膜蛋白34 包涵体圆二色谱凋亡

Corrigendum to“Generation of Leydig-like cells:approaches,characterization,and challenges”

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2023年第6期25卷 756-756页

作者： Zhao-Hui Li Jun-Dong Lu Shi-Jun Li Hao-Lin Chen zhi-jian su Guangdong Provincial Key Laboratory of Bioengineering Medicine Department of Cell BiologyJinan UniversityGuangzhou 510632China Institute of Life Sciences Wenzhou UniversityWenzhou 325035China Department of Gynecology and Obstetrics The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027China National Engineering Research Center of Genetic Medicine Jinan UniversityGuangzhou 510632China

In the published article by Li et al.,1 an error occurred in the *** funding agency should be“the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515010947)”,not“the Natural Science Foundation of Gua... 详细信息

关键词： Basic Guangdong funding

Physical Layer Security Scheme With AoI-Awareness for Industrial IoT Based on Covert Communications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2025年第1期 276-278页

作者： Yaping Li zhi-Xin Liu Jia-Wei su Ya-Zhou Yuan the Key Laboratory of Industrial Computer Control Engineering of Hebei Province Yanshan University

Dear Editor,Industrial Internet of things(IIoT) is a typical application of cyberphysical system(CPS). In the IIoT, wireless communication is an inevitable trend to replace the deployment-limited wired transmission for cases with large-scale and mobile devices. However, wireless communication gives rise to critical issues related to physical security, such as malicious detections and attacks [1].

关键词：

Quantum public-key crypto via EPR pairs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： Xiang-Qun Fu Hong-Wei Li jian-Hong Shi Tan Li Wan-su Bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs