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Testis-specific Fankl gene in knockdown mice produces oligospermia via apoptosis

Asian Journal of Andrology 2014年第1期16卷 124-130页

作者： Wan-Wei Dong hua-Liang huang Wei Yang Jia Liu Yang Yu Sheng-Lai Zhou Wei Wang Xiang-Chuan Lv Zhao-Yang LP Mei-Ying Zhang zhi-Hong Zheng Wei Yan Laboratory Animal Centre China Medical University Shenyang China Department of Clinical Laboratory The Third Affiliated Hospital of Inner Mongonia MedicalUniversity Baogang Hospital Baotou China Department of Pathology and Pathophysiology Research China Medical University Shenyang China and Department ofPhysiology and Cell Biology University of Nevada School of Medicine Reno Nevada USA.

Fankl is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis. A reduction in sperm number and an increase in apoptotic spermatocytes were observed in Fankl-knockdown mice, and the apoptotic cells were found to be primarily spermatogonia and spermatocytes. The CAST results demonstrated that the consensus DNA-binding sequence was AAAAAG, in which the percentage occurrence of each base at each position ranged from 55 to 86%. This sequence was present in the promoter regions of 10 differentially expressed genes that were examined using microarray analysis. In total, 17 genes were differentially expressed with changes in their expression levels greater than twofold. The abnormal expression of Fankl target genes that were regulated directly or indirectly by Fankl reduced the number of sperm in the knockdown mice. Thus, FANK1 may Dlav a pivotal role in sDermato^enesis as a transcription *** is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis, A reduction in sperm number an

关键词： Fankl knockdown mice spermatogenesis

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Small-molecule 7,8-dihydroxyflavone counteracts compensated and decompensated cardiac hypertrophy via AMPK activation

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Journal of Geriatric Cardiology 2022年第11期19卷 853-866页

作者： Peng-Zhou HANG Pei-Feng LI Jie LIU Feng-Feng LI Ting-Ting CHEN Yang PAN Man-Ru ZHANG hua-Qing YU Hong-Yu JI zhi-Min DU Jing ZHAO Institute of Clinical Pharmacology the Second Affiliated Hospital of Harbin Medical University(University Key Laboratory of Drug ResearchHeilongjiang Province)HarbinChina Department of Pharmacy Clinical Medical CollegeYangzhou UniversityNorthern Jiangsu People's HospitalYangzhouChina Department of Pharmacology Harbin Medical UniversityHarbinChina State Key Laboratory of Quality Research in Chinese Medicines Macao University of Science and TechnologyMacaoChina Department of Cardiology the First Affiliated Hospital of Harbin Medical UniversityHarbinChina

BACKGROUND Pathological cardiac hypertrophy is a compensated response to various stimuli and is considered a key risk factor for heart failure.7,8-Dihydroxyflavone(7,8-DHF)is a flavonoid derivative that acts as a small-molecule brain-derived neurotrophic factor *** present study aimed to explore the potential role of 7,8-DHF in cardiac *** Kunming mice and H9c2 cells were exposed to transverse aortic constriction or isoproterenol(ISO)with or without 7,8-DHF,respectively.F-actin staining was performed to calculate the cell *** levels of hypertrophic markers,including ANP,BNP,andβ-MHC,were ***,hematoxylin-eosin staining,and transmission electron microscopy were used to examine the cardiac function,histology,and ultrastructure of *** levels of mitochondria-related factors,such as adenosine monophosphate-activated protein kinase(AMPK),and peroxisome proliferator-activated receptorγcoactivator-1α(PGC-1α),were *** 7,8-DHF inhibited compensated and decompensated cardiac hypertrophy,diminished the cross-sectional area,and alleviated the mitochondrial disorders of ***,7,8-DHF reduced the cell size and repressed the mRNA levels of the hypertrophic markers of ISO-treated *** addition,7,8-DHF activated AMPK and PGC-1αsignals without affecting the protein levels of mitochondrial dynamics-related *** effects of 7,8-DHF were eliminanted by Compound C,an AMPK *** These findings suggest that 7,8-DHF inhibited cardiac hypertrophy and mitochondrial dysfunction by activating AMPK signaling,providing a potential agent for the treatment of pathological cardiac hypertrophy.

关键词： cardiac hypertrophy inhibited

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Effect of axial vertical vibration on degeneration of lumbar intervertebral discs in modified bipedal rats: An in-vivo study

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 781-784页

作者： Xiao Liang Hao Shen Wei-Dong Shi Shan Ren Wei Jiang Hao Liu Peng Yang zhi-Yong sun Jun Lin Hui-Lin Yang Department of Orthopaedic Surgery The First Affiliated Hospital of Soochow University Intensive Care Unit Department The First Affiliated Hospital of Medical CollegeShihezi University Department of Nephrology The Affiliated Hospital of Qingdao University

Objective: To assess the effects of axial vibrations on gene expression and lumbar intervertebral disc degeneration in vivo. Methods: A modified bipedal rat model was established using a brachial plexus rhizotomy approach to imitate human upright posture. The experimental animals were randomly divided into three groups: control, vertical vibration, and whole-body vibration. Gene expression in degeneration of the intervertebral discs was assessed by reverse transcription-quantitative polymerase chain reaction. Results: The expression of aggrecan, Col1α1, Col2α1, and decorin were shown to be up-regulated in 14-week-old rats in the vertical vibration and whole-body vibration groups, whereas biglycan and versican expression was down-regulated in 14-week-old rats of the two experimental groups. Furthermore, biglycan and versican expression levels were shown to be lower in the whole-body vibration group than in the vertical vibration group(P<0.05). Conclusions: This in-vivo study demonstrated that vibrations can influence the expression of anabolic genes. Furthermore, whole-body vibrations seem to have a greater effect in this regard than vertical vibrations. A new method is expected to relieve the low back pain of the patients through our research.

关键词： Intervertebral disc Vibration Animal model Gene expression

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Identification of the Genetic Cause for Childhood Disintegrative Disorder by Whole-Exome Sequencing

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Neuroscience Bulletin 2017年第2期33卷 251-254页

作者： Zhu Wen Tian-Lin Cheng Da-zhi Yin Shi-Bang sun Zheng Wang Shun-Ying Yu Yi Zhang Zilong Qiu Ya-Song Du Shanghai Mental Health Center Shanghai Jiao Tong University School of MedicineShanghai 200030China Institute of Neuroscience Center for Excellence in BrainScience and Intelligence TechnologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China School of Life Sciences Peking UniversityBeijing 100871China Euler Genomics Beijing 102206China

Dear Editor,Childhood Disintegrative Disorder（CDD）,also known as Heller’s syndrome and disintegrative psychosis,is a rare progressive neurological disorder,characterized by a late onset（[2 years of age）and regres... 详细信息

关键词： Identi?cation of the Genetic Cause for Childhood Disintegrative Disorder by Whole-Exome Sequencing IIIA CDD

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纳米Cu/3Y-ZrO_2复合材料的制备及脱硫性能研究

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功能材料 2007年第8期38卷 1366-1369页

作者：汪慧峰顾华志汪厚植武汉科技大学高温陶瓷与耐火材料湖北省重点实验室湖北武汉430081

研究了不同制备方法得到的Cu/3Y-ZrO2纳米材料的结构和物相变化,并在模拟烟气中采用气相色谱仪对其脱除SO2的性能进行了比较研究。结果表明,以氨水为沉淀剂,采用共沉淀-超临界干燥法所得的产物晶形发育较好且团聚较少,但经真空热处理后... 详细信息

研究了不同制备方法得到的Cu/3Y-ZrO2纳米材料的结构和物相变化,并在模拟烟气中采用气相色谱仪对其脱除SO2的性能进行了比较研究。结果表明,以氨水为沉淀剂,采用共沉淀-超临界干燥法所得的产物晶形发育较好且团聚较少,但经真空热处理后,在铜负载量为30%以上时才出现以金属Cu形式存在的铜组分衍射峰;而以碳酸钠为沉淀剂,采用分步沉淀-真空干燥处理后的产物晶形发育较差,团聚程度也增加,在相同的热处理条件下,铜负载量为10%以上就出现了CuO的衍射峰。与分步沉淀-真空干燥法得到的产物相比,共沉淀-超临界干燥产物的脱硫率较高,稳定性也较好。

关键词：纳米材料氧化铜氧化锆制备脱硫

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Stimulated Brillouin interaction between guided phonons and photons in a lithium niobate waveguide

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 173-175页

作者： Yuan-Hao Yang Jia-Qi Wang Zheng-Xu Zhu Xin-Biao Xu Qiang Zhang Juanjuan Lu Yu Zeng Chun-hua Dong Luyan sun Guang-Can Guo Chang-Ling Zou CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Center for Quantum Information Institute for Interdisciplinary Information SciencesTsinghua UniversityBeijing 100084China

The Brillouin scattering is one of the strongest nonlinear optical effects in dielectrics[1].In particular,the backward Brillouin scattering(BBS)provides an efficient method to achieve low-noise optical gain for active photonics[2]and the coherent interconnects between 10 GHz phonons and photons[3].However,previous studies on BBS are primarily limited to the unconfined phonons[1]or phonon confined in suspended structures[4-6].

关键词： scattering waveguide Brillouin

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煤矿自动控制风门的选择与应用

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煤矿机械 2006年第8期27卷 134-136页

作者：孙建华邵芝梅黑龙江科技学院哈尔滨150027

通过对目前煤矿使用的自动控制风门的原理进行分析,指出各种自动控制风门的优缺点及在使用选择过程中的注意事项,并重点介绍了PLC控制器在煤矿自动控制风门设计中的重要性。

关键词：自动控制风门信号拾取 PLC控制器

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一种基于变长位平面的自适应视频水印算法研究

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计算机应用研究 2010年第5期27卷 1876-1878页

作者：蒋华孙志刚孙晋永桂林电子科技大学计算机与控制学院广西桂林541004

针对视频水印嵌入强度在平衡水印系统的鲁棒性和不可见性时存在的问题,提出了一种变长位平面分解的视频水印算法。该算法根据8位灰度水印图像在位平面分解后各位平面在重构水印图像时的权值不同,将不同的位平面嵌入在不同数量的DCT中低... 详细信息

针对视频水印嵌入强度在平衡水印系统的鲁棒性和不可见性时存在的问题,提出了一种变长位平面分解的视频水印算法。该算法根据8位灰度水印图像在位平面分解后各位平面在重构水印图像时的权值不同,将不同的位平面嵌入在不同数量的DCT中低频系数中,并利用HVS和Watson视觉模型的亮度掩蔽特性自适应的调整水印嵌入的强度从而实现了自适应地嵌入水印。实验表明,该视频水印系统在满足鲁棒性的同时具有高度的透明性。

关键词：变长位平面人类视觉系统视频水印自适应性

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Right-sided colon cancer and left-sided colorectal cancer respond differently to cetuximab

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癌症 2015年第9期34卷 384-393页

作者： Feng Wang Long Bai Tian-Shu Liu Yi-Yi Yu Ming-Ming He Kai-Yan Liu Hui-Yan Luo Dong-Sheng Zhang Yin Jin Feng-hua Wang zhi-Qiang Wang De-Shen Wang Miao-Zhen Qiu Chao Ren Yu-Hong Li Rui-hua Xu Department of Medical Oncology Sun Yat-sen University Cancer Center:State Key Laboratory of Oncology in South China Department of Medical Oncology Zhongshan HospitalFudan University Collaborative Innovation Center for Cancer Medicine

Introduction: Right-sided colon cancer(RSCC) and left-sided colorectal cancer(LSCRC) differ with respect to their biology and genomic patterns. This study aimed to examine whether the primary tumor location is associated with the response to cetuximab in patients with metastatic colorectal cancer(m CRC).Methods: Patients with mC RC treated with cetuximab and standard chemotherapy as first- or second-line treatments were compared with randomly chosen patients who were treated with chemotherapy alone between 2005 and *** main outcome measures were the overal response rate(ORR), progression-free survival(PFS), and overal survival(OS).The differences in the outcome were analyzed by using the chi-squared test, Student’s t test, and Kaplan-Meier ***: The treatment results of 206 patients with m CRC treated with cetuximab and standard chemotherapy as first- or second-line treatments were compared with those of 210 patients who were treated with chemotherapy alone. As a first-line treatment, cetuximab with chemotherapy was associated with a significantly higher ORR(49.4 % vs. 28.6 %, P = 0.005) as well as longer PFS(9.1 vs. 6.2 months, P = 0.002) and OS(28.9 vs. 20.1 months,P = 0.036) than chemotherapy alone in patients with LSCRC. However, cetuximab neither improved the ORR(36.4 % vs. 26.2 %, P = 0.349) nor prolonged PFS(5.6 vs. 5.7 months, P = 0.904) or OS(25.1 vs. 19.8 months, P = 0.553) in patients with RSCC. As a second-line treatment, cetuximab exhibited a tendency to improve the ORR(23.5 % vs. 10.2 %,P = 0.087) and prolong PFS(4.9 vs. 3.5 months, P = 0.064), and it significantly prolonged OS(17.1 vs. 12.4 months,P = 0.047) compared with chemotherapy alone in the patients with LSCRC. In contrast, as a second-line treatment,cetuximab neither improved the ORR(7.1 % vs. 11.4 %, P = 0.698) nor prolonged PFS(3.3 vs. 4.2 months, P = 0.761) or OS(13.4 vs. 13.0 months, P = 0.652) in patients with ***: The addition of cetuximab to chemotherapy in b

关键词： Colorectal neoplasms Cetuximab Left-sided Right-sided Chemotherapy

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以二斑叶螨为食的伪钝绥螨种群生命表

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贵州农业科学 2009年第1期37卷 85-87页

作者：孙月华郅军锐贵州大学昆虫研究所贵州贵阳550025 贵州山地农业病虫害重点实验室贵州贵阳550025

研究了在31℃条件下伪钝绥螨取食二斑叶螨时的发育和繁殖情况，建立了伪钝绥螨试验种群生命表，以期为利用该螨进行生物防治提供科学依据。试验结果表明，31℃时伪钝绥螨的净增殖率17．8594，世代平均周期11．6375d，内禀增长率0．2477... 详细信息

研究了在31℃条件下伪钝绥螨取食二斑叶螨时的发育和繁殖情况，建立了伪钝绥螨试验种群生命表，以期为利用该螨进行生物防治提供科学依据。试验结果表明，31℃时伪钝绥螨的净增殖率17．8594，世代平均周期11．6375d，内禀增长率0．2477，周限增长率1．2811，种群倍增所需时间2．7983d，日平均产卵量2．5974粒／d，总产卵量27～65粒／雌，平均40．1250粒／雌。

关键词：伪钝绥螨二斑叶螨试验种群生命表

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