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Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits

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Horticulture Research 2021年第1期8卷 368-377页

作者： zhi-zhong song Bin Peng Zi-Xia Gu Mei-Ling Tang Bei Li Mei-Xia Liang Li-Min Wang Xiao-Tong Guo Jian-Ping Wang Yu-Fen Sha Hong-Xia Zhang The Engineering Research Institute of Agriculture and Forestry Ludong University186 Hongqizhong RoadYantai 264025China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong(Ludong University) 186 Hongqizhong RoadYantai 264025China Institute of Botany Jiangsu Province and Chinese Academy of Sciences1 QianhuhoucunNanjing 210014China Yantai Academy of Agricultural Science 26 Gangcheng West StreetYantai 265500China

The aroma of peach fruit is predominantly determined by the accumulation of γ-decalactone and ester compounds.A previous study showed that the biosynthesis of these aroma compounds in peach fruit is catalyzed by PpAAT1,an alcohol *** this work,we investigated the key active site residues responsible for γ-decalactone and ester biosynthesis.A total of 14 candidate amino acid residues possibly involved in internal esterification and 9 candidate amino acid residues possibly involved in esterification of PpAAT1 were assessed via site-directed *** of the in vitro enzyme activities of PpAAT1 and its site-directed mutant proteins(PpAAT1-SMs)with different amino acid residue mutations as well as the contents of γ-decalactone in transgenic tobacco leaves and peach fruits transiently expressing PpAAT1 and PpAAT1-SMs revealed that site-directed mutation of H165 in the conserved HxxxD motif led to lost enzymatic activity of PpAAT1 in both internal esterification and its reactions,whereas mutation of the key amino acid residue D376 led to the total loss ofγ-decalactone biosynthesis activity of *** of 9 and 7 other amino acid residues also dramatically affected the enzymatic activity of PpAAT1 in the internal esterification and esterification reactions,*** findings provide a biochemical foundation for the mechanical biosynthesis ofγ-decalactone and ester compounds catalyzed by PpAAT1 in peach fruits,which could be used to guide the molecular breeding of new peach species with more favorable aromas for consumers.

关键词： residues directed alcohol

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TWO NEW NEOLIGNANS FROM MESSERSCHMIDIA SIBIRICA ***.

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Chinese Chemical Letters 1992年第12期3卷 975-976页

作者： zhi zhong song Zi Min LIU zhong Jian JIA Qi Xiu ZHU Institute of Organic Chemistry,State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou-730000

From the aerial parts of Messerschmidia sibirica ***.,two new neolignans were isolated and elucidated as messerschmidin and its acetate.

关键词： TWO NEW NEOLIGNANS FROM MESSERSCHMIDIA SIBIRICA L.SSP ppm

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CHARACTERISTICS OF MICROBIAL TRANSFUSION IN POROUS MEDIUM AND ITS THEORETICAL INVESTIGATION

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Journal of Hydrodynamics 2003年第2期15卷 101-107页

作者： Lei Guang lun, Zhang zhong zhi, song Shao fu, Wang Hong jun, Li Shu yuanFaculty of Chemical Engineering, University of Petroleum, Beijing 102249, China Faculty of Chemical Engineering University of Petroleum Beijing 102249 China

This paper introduces the experimental and theoretical results of microbialtransfusion in porous medium. The experimetnal results show that microbes can pass through throatsof porous media which permeability is 0. 03 Darcy or over. Based on these results a new supplemorphotropy and dynamic directionality theory is obtained to explain this transfusion *** the characteristics of convection and diffusion while microbe is moving, one dimensionaland radial transfusion mathematical model including adsorption has been set up. With this model andexperimental data, the diffusion coefficient and maximum adsorption constants are obtained bycalculation and mathematical fitting. The results show that the concentration of microbe decreasesquickly during transfusion, and the effective region also decreases. Therefore, it is indispensablefor microbes to reproduce quickly under the reservoir conditions. Additionally, this model can alsobe used to calculate the time when the oil production begins to increase and when the microbes aregot from another production well.

关键词： microbial transfusion porous medium microbial enhanced oil recovery

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A large-scale fatal outbreak of Pasteurella multocida among wild rodents in southwestern China

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Journal of Biosafety and Biosecurity 2020年第2期2卷 91-98页

作者： Chun-Hong Du Yong Huang Jia-Fu Jiang Zi-Hou Gao Na Jia Shan Yang Chao-Nian Chen Shun-Gao Ma Yu-Ying Liang Bao-Gui Jiang Wei-Hong Sang Peng Wang Yun Liang Yun-zhi Zhang Ji-Hua Zhou Shui-Ping Chen Frans Jongejan zhi-zhong song Yi-Gang Tong Wu-Chun Cao State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingPR China Yunnan Institute for Endemic Diseases Control and Prevention Dali YunnanPR China Puer Center for Disease Control and Prevention PuerYunnanPR China Simao Prefecture Center for Disease Control and Prevention PuerYunnanPR China DaLi Bai Autonomous Prefecture People’Hospital YunnanPR China Fifth Medical Center of PLA General Hospital BeijingPR China Utrecht Centre for Tick-borne Diseases(UCTD) Faculty of Veterinary MedicineUtrecht UniversityYalelaan 13584 CL UtrechtThe Netherlands College of Life Science and Technology Beijing University of Chemical Technology.BeijingPR China

Background:Pasteurella multocida is an important and old zoonotic pathogen worldwide which has an impressive host spectrum including numerous domestic and wild animals as well as birds,causing specific diseases or outbreak with great economic *** has never been reported that *** can cause an epidemic in wild *** June 5–17,2016,more than 1000 rodent deaths of an unknown cause quickly spread in the PuEr City,Yunnan province,southwestern ***:The rodents in affected areas and outside of the epidemic areas were collected and screened for possible known pathogens including Yersinia pestis,rabies virus and hantavirus as well as other *** possible bacterial pathogens were isolated both by culture medium and by mouse inoculation in *** isolates were identified by the Vitek GNI card and PCR assays for 16S rRNA *** pathogen strains were selected for whole genome sequencing ***:A total of 123 rodents were collected from 25 sample sites at affected area,among of which,all 119 dead rodents were negative for the pathogen under consideration except ***,and all four live rodents were negative for *** addition,480 rodents collected from other 23 counties outside of the epidemic area in Yunnan were negative for with ***.A total of 14 strains of ***(six directly isolated from the field rodents and eight from the experimental mice that were injected with the organ substrates from the dead rodents)belonged to serogroup A and serogroup F represented by 9 N and 20 N were identified in these epidemic *** genome sequencing revealed that the serogroup F strain shared 99%similarity to *** Pm70 from chicken,but contained a 50 k bp insertion *** serogroup A strain shared 95%similarity to *** FDAARGOS_385 from a human patient,but contained four large structural *** abnormalities were identified in the livers,lungs,hearts and brains of the ino

关键词： Pasteurella multocida Serotypes A Serotypes F Rodents Outbreak China

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宣城水东地区中二叠统硅质角砾岩地球化学特征及其成因环境研究

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矿物岩石地球化学通报 2018年第4期37卷 687-695页

作者：王存智黄志忠宋世明陈志洪中国地质调查局南京地质调查中心

为研究长江中下游成矿带东部宣城水东地区中二叠统孤峰组中发育的一套硅质岩特别是其中的硅质角砾岩的成因,在详细的野外地质调查和室内岩石学研究基础上,对取自该套硅质岩中的7件硅质角砾岩、7件块状硅质岩和1件中薄层硅质岩样品进行... 详细信息

为研究长江中下游成矿带东部宣城水东地区中二叠统孤峰组中发育的一套硅质岩特别是其中的硅质角砾岩的成因,在详细的野外地质调查和室内岩石学研究基础上,对取自该套硅质岩中的7件硅质角砾岩、7件块状硅质岩和1件中薄层硅质岩样品进行了主量元素、微量元素和稀土元素地球化学分析。结果表明,硅质角砾岩和硅质岩的地球化学特征相似,其SiO_2含量高（93.70%-99.24%）、Al/（Al＋Fe＋Mn）值较高、轻稀土略富集、无明显铈异常和铕异常,显示非热液成因的特征。硅质岩的地球化学特征指示其主要为正常海水沉积成因,形成于靠近大陆边缘的沉积环境,受一定的陆源碎屑输入影响。硅质角砾岩的形成可能与江南断裂带活动影响有关,后期的海底热液对硅质岩进行叠加或动力学改造,并在局部形成角砾状构造。

关键词：硅质角砾岩地球化学中二叠统沉积环境宣城

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基于预测控制的LLC变换器谐振频率自动跟踪技术

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燕山大学学报 2016年第5期40卷 419-425页

作者：阚志忠张海松齐飞燕山大学电气工程学院河北秦皇岛066004

LLC谐振变换器在工作时,谐振元器件参数可能会出现波动,影响LLC谐振变换器的电压增益特性。在定频占空比控制方式下,上述现象造成LLC谐振变换器的设计谐振频率与开关频率不一致,同时降低LLC谐振变换器的效率。针对这一问题,本文提出一... 详细信息

LLC谐振变换器在工作时,谐振元器件参数可能会出现波动,影响LLC谐振变换器的电压增益特性。在定频占空比控制方式下,上述现象造成LLC谐振变换器的设计谐振频率与开关频率不一致,同时降低LLC谐振变换器的效率。针对这一问题,本文提出一种基于预测控制的LLC谐振频率追踪控制方法,此控制方法克服了不能在轻载条件下跟踪谐振频率的问题,不仅能够有效抵抗谐振元件参数变化的扰动,而且具有算法简单和采样环节简便易于实现的优点。最后给出MATLAB仿真结果,验证了所提方法的可行性。

关键词： LLC谐振变换器自动频率追踪预测控制定频控制

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Polarization-encoded neural networks with simplified grating patch

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Science China Technological Sciences 2025年

作者： Chengyan zhong Xiang WANG Lingfei LI Yuanchi CUI Lei XIAO Dawei song Junxiong GUO Wen HUANG Yufeng GUO Yu LIU College of Integrated Circuit Science and Engineering Nanjing University of Posts and Telecommunications National and Local Joint Engineering Laboratory of RF Integration and Micro-Assembly Technology College of Integrated Circuits Hangzhou Global Scientific and Technological Innovation Centre Zhejiang University Nanjing Mumusili Technology Co. LTD School of Integrated Circuits Tsinghua University Institute of Advanced Study School of Electronic Information and Electrical Engineering Chengdu University State Key Laboratory of Electronic Thin Films and Integrated Devices School of Integrated Circuit Science and EngineeringUniversity of Electronic Science and Technology of China

Optical neural networks (ONNs) offer a promising solution for high-performance, energy-efficient artificial intelligence hardware by leveraging the parallelism and speed of light. However, the large-scale implementation of ONNs remains challenging due to the bulky footprint and complex control of optical synapses. In this work, we propose and simulate a plasmonic polarized synaptic architecture that overcomes the diffraction limit and enables ultra-compact ONNs. By tuning the polarization state of incident light, the optical transmittance through each plasmonic unit can be dynamically adjusted to represent a synaptic weight. Our plasmonic structures, with features as small as 40 nm, operate well below this limit in the visible spectrum (400–750 nm). Compared with diffraction and interference-based circuit designs, our proposed method achieves a substantial reduction in synaptic density by factors of 150000-fold and 1500-fold, respectively. Furthermore, we successfully demonstrate a proof-of-concept plasmonic ONN applied to the Canadian Institute for Advanced Research—10classes (CIFAR-10) dataset using a Visual Geometry Group network with 16 layers (VGG16) model. After training for 80epochs, the network achieves an accuracy of 93%. The polarization-tunable plasmonics paves the way towards scalable ONNs for next-generation artificial intelligence (AI) accelerators and smart sensors.

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Impacts of the annual cycle of background SST in the tropical Pacific on the phase and amplitude of ENSO

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 12-17页

作者： song Jiang Congwen Zhu Ning Jiang State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological SciencesBeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

The dominant annual cycle of sea surface temperature(SST)in the tropical Pacific exhibits an antisymmetric mode,which explains 83.4%total variance,and serves as a background of El Niño-Southern Oscillation(ENSO).However,there is no consensus yet on its anomalous impacts on the phase and amplitude of *** on data during 1982-2022,results show that anomalies of the antisymmetric mode can affect the evolution of ENSO on the interannual scale via Bjerknes feedback,in which the positive(negative)phase of the antisymmetric mode can strengthen El Niño(La Niña)in boreal winter via an earlier(delayed)seasonal cycle transition and larger(smaller)annual *** magnitude of the SST anomalies in the equatorial eastern Pacific can reach more than±0.3◦C,regulated by the changes in the antisymmetric mode based on random sensitivity *** reveal the spatial pattern of the annual cycle associated with the seasonal phase-locking of ENSO evolution and provide new insight into the impact of the annual cycle of background SST on ENSO,which possibly carries important implications for forecasting ENSO.

关键词： Annual cycle SST anomaly Antisymmetric mode ENSO

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： song He Li Li Sai Wang Shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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Neuronal regulated cell death in aging-related neurodegenerative diseases:key pathways and therapeutic potentials

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Neural Regeneration Research 2025年第8期20卷 2245-2263页

作者： Run song Shiyi Yin Jiannan Wu Junqiang Yan Department of Neurology The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina Neuromolecular Biology Laboratory The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina

Regulated cell death(such as apoptosis,necroptosis,pyroptosis,autophagy,cuproptosis,ferroptosis,disulfidptosis)involves complex signaling pathways and molecular effectors,and has been proven to be an important regulatory mechanism for regulating neuronal aging and ***,excessive activation of regulated cell death may lead to the progression of aging-related *** review summarizes recent advances in the understanding of seven forms of regulated cell death in age-related ***,the newly identified ferroptosis and cuproptosis have been implicated in the risk of cognitive impairment and neurodegenerative *** forms of cell death exacerbate disease progression by promoting inflammation,oxidative stress,and pathological protein *** review also provides an overview of key signaling pathways and crosstalk mechanisms among these regulated cell death forms,with a focus on ferroptosis,cuproptosis,and *** instance,FDX1 directly induces cuproptosis by regulating copper ion valency and dihydrolipoamide S-acetyltransferase aggregation,while copper mediates glutathione peroxidase 4 degradation,enhancing ferroptosis ***,inhibiting the Xc-transport system to prevent ferroptosis can increase disulfide formation and shift the NADP^(+)/NADPH ratio,transitioning ferroptosis to *** insights help to uncover the potential connections among these novel regulated cell death forms and differentiate them from traditional regulated cell death *** conclusion,identifying key targets and their crosstalk points among various regulated cell death pathways may aid in developing specific biomarkers to reverse the aging clock and treat age-related neurodegenerative conditions.

关键词： apoptosis autophagy cuproptosis disulfidptosis ferroptosis necroptosis neurodegenerative disease neurological aging diseases PANoptosis pyroptosis

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