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Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells

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World Journal of Gastroenterology 2005年第44期11卷 7033-7035页

作者： zhi-zhong liu Wen-Ying Huang Ju-Sheng Lin Xiao-Sheng Li Kuo-Huan Liang Jia-Long Huang Institute of Liver Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China The People's Hospital of Chenzhou City Chenzhou 423000 Hunan Province China Xiehe Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Hubei Province China

AIM： To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells. METHODS： All tumor tissue samples were obtained from 39 hepatocarcinoma patients with a mean age of 49.6 years （range 22-76 years）. We divided the samples into irradiated group and non-irradiated group and measured their plating efficiency （PE）, population doubling time （PDT）, radiosensitivity index SF2 and cell RESULTS： The PDT of primary culture of hepatocardnoma cells was 91.0±6.6 h, PE was 12.0±1.4%, SF2 was 0.41±0.05%. The PDT of their inadiated progeny was 124.8±5.8 h, PE was 5.0±0.7%, SF2 was 0.65±0.09%. The pdmary cultured human hepatocarcinoma cells showed significant S reduction and G^2 arrest in a dose-dependent manner. The progeny of irradiated primary cultured hepatocarcinoma cells grew more slowly and its radiosensitivity increased. CONCLUSION： The progeny of irradiated primary cultured human hepatocarcinoma cells grows more slowly and its radiosensitivity increases.

关键词： Hepatocarcinoma Cell cycle Population doubling time Radiosensitivity

Prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and micronucleus assay

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World Journal of Gastroenterology 2005年第44期11卷 7036-7039页

作者： zhi-zhong liu Wen-Ying Huang Xiao-Sheng Li Ju-Sheng Lin Xiao-Kun Cai Kuo-Huang Lian He-Jun Zhou Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Hubei Province China The People's Hospital of Chenzhou City Chenzhou 423000 Hunan Province China Xiehe Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Hubei Province China

AIM： To investigate the prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and rnicronucleus assay. METHODS： Clonogenic assay, flow cytometry, and CB micronuclei assay were used to survey the cell survival rate, radiation-induced apoptosis and rnicronucleus frequency of hepatocarcinorna cell lines SMMC-7721, HL-7702, and HepG2 after being irradiated by X-ray at the dosage ranging 0-8 Gy. RESULTS： After irradiation, there was a dose-effect relationship between rnicronucleus frequency and radiation dosage among the three cell lines （P〈0.05）. A positive relationship was observed between apoptosis and radiation dosage among the three cell lines. The HepG2 cells had a significant correlation （P〈0.05） but apoptosis incidence had a negative relationship with rnicronucleus frequency. There was a positive relationship between apoptosis and radiation dosage and the correlation between 5MMC-7721 and HL-7702 cell lines had a significant difference （P〈0.01）. After irradiation, a negative relationship between cell survival rate and radiation dosages was found among the three cell lines （P〈0.01）. There was a positive relationship between cell survival rate and rnicronucleus frequency （P〈0.01）. No correlation was observed between apoptosis and cell survival rate. CONCLUSION： The radiosensiUvity of hepatocarcinoma cells can be reflected by apoptosis and rnicronuclei. Detection of apoptosis and rnicronuclei could enhance the accuracy for predicting radiosensitivity.

关键词： Hepatocarcinoma cell ticronuclei Apoptosis Radiosensitivity

Cell survival curve for primary hepatic carcinoma cells and relationship between SF2 of hepatic carcinoma cells and radiosensitivity

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World Journal of Gastroenterology 2005年第44期11卷 7040-7043页

作者： zhi-zhong liu Wen-Ying Huang Ju-Sheng Lin Xiao-Sheng Li Xiao Lan Xiao-Kun Cai Kuo-Huan Liang Hai-Jun Zhou Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China The People's Hospital of Chenzhou Chenzhou 423000 Hunan Province China Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To establish the cell survival curve for primary hepatic carcinoma cells and to study the relationship between SF2 of primary hepatic carcinoma cells and radiosensitivity. METHODS： Hepatic carcinoma cells were cultured in vitro using 39 samples of hepatic carcinoma at stages Ⅱ-Ⅳ. Twenty-nine samples were cultured successfully in the fifth generation cells. After these cells were radiated with different dosages, the cell survival ratio and SF2 were calculated by clonogenic assay and SF2 model respectively. The relationship between SF2 and the clinical pathological feature was analyzed. RESULTS： Twenty-nine of thirty-nine samples were successfully cultured. After X-ray radiation of the fifth generation cells with 0, 2, 4, 6, 8 Gy, the cell survival rate was410, 36.5%, 31.0%, 26.8%, and 19%, respectively. There was a negative correlation between cell survival and irradiation dosage （r = -0.973, P〈0.05）. SF2 ranged 0.28-0.78 and correlated with the clinical stage and pathological grade of hepatic carcinoma （P〈0.05）. There was a positive correlation between SF2 and D0.5 （r = 0.773, P〈0.05）. CONCLUSION： SF2 correlates with the clinical stage and pathological grade of hepatic carcinoma and is a marker for predicting the radiosensitivity of hepatic carcinomas.

关键词： Hepatocarcinoma SF2 Radiosensitivity D0.54

Study on High-Performance Computing for Simulation of End Milling Force

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厦门大学学报（自然科学版） 2002年第S1期41卷 183-184页

作者： ZHANG zhi-hai, ZHENG Li, LI zhi-zhong, liu Da-cheng, ZHAN G Bo-peng (Department of Industry Engineering, Tsinghua University, Beijing 1000 84, China)

Milling Process Simulation is one of the important re search areas in manufacturing science. For the purpose of improving the prec ision of simulation and extending its usability, numerical algorithm is more and more used in the milling modeling areas. But simulative efficiency is decreasin g with increase of its complexity. As a result, application of the method is lim ited. Aimed at above question, high-efficient algorithm for milling process sim ulation is studied. It is important for milling process simulation’s applicatio n. Parallel computing is widely used to solve the large-scale computation question s. Its advantages include system flexibility, robust, high-efficient computing capability and high ratio of performance to price. With the development of compu ter network, utilizing the computing resource in the Internet, a virtual computi ng environment with powerful computing capability can be consisted by microc omputers, and the difficulty of building hardware environment which is used to s upport parallel computing is reduced. How to use network technology and parallel algorithm to improve simulative effic iency for milling forces simulation is investigated in the paper. In order to pr edict milling forces, a simplified local milling forces model is used in the pap er. End milling cutter is assumed to be divided by r number of differential elem ents along the axial direction of the cutter. For a given time, the total cuttin g forces can be obtained by summarizing the resultant cutting force produced by each differential cutter disc. Divide the whole simulative time into some segmen ts, send these program’s segments to microcomputers in the Internet and obtain the result of the program’s segments, all of the result of program’s segments a re composed the final result. For implementing the algorithm, a distributed Parallel computing framework is de signed in the paper. In the framework, web server plays a role of controller. Us ing Java RMI(remote method int

关键词： end-milling force model simulation high-perfo rmance computing parallel algorithm Java RMI

Coronary sinus： an important target of catheter ablation of atrial fibrillation

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Chinese Medical Journal 2009年第23期122卷 2912-2913页

作者： ZHANG Ming LI zhi-zhong liu Xin-peng DONG Jian-zeng MA Chang-sheng Department of cardiology Beijing Anzhen Hospital CapitalMedical University Beijing 100029 China

The pulmonary veins have been demonstrated to be the major source of atrial fibrillation （AF）. Isolation of the pulmonary veins is effective in curing patients with paroxysmal AE However, this strategy is associated with limited success in some patients with paroxysmal AF and in most patients with persistent and permanent AF It requires more widespread ablation targeting most parts of the left atrial and thoracic veins and may include some discrete sites, which, if missed or neglected, can lead to persistence of fibrillation. In recent years, other AF origins have also been reported in addition to some types of atrial tachycardia and AF originating from the coronary sinus （CS） musculature. The CS appears to be a major element perpetuating persistent AF.

关键词： coronary sinus atrial fibrillation catheter ablation

Improving native human sperm freezing protection by using a modified vitrification method

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Asian Journal of Andrology 2021年第1期23卷 91-96页

作者： Dai Zhou Xing-Ming Wang Rui-Xue Li Yi-Ze Wang Yuan-Chi Chao zhi-zhong liu Zeng-Hui Huang Hong-Chuan Nie Wen-Bing Zhu Yue-Qiu Tan Li-Qing Fan Hnstitute of Reproduction and Stem Cell Engineering School of Basic Medicine ScienceCentral South UniversityChangsha 410000China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410000China Department of Urology Hunan Cancer HospitalChangsha 410000China

Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa in clinical ***,it has been shown to have a negative impact on sperm function and *** as a successful alternative method has been proved to have better protective effects on human embryos,but vitrification of spermatozoa is still subject to low recovery *** this study,a modified vitrification method for native spermatozoa was developed.A total of 28 semen samples were included;each sample was divided into three equal parts and assigned to fresh,slow freezing,and vitrification *** vitality,motility,morphology,DNA integrity,and acrosome reaction were assessed for each of the *** results showed that vitrification achieves better results for several sperm protection parameters than slow freezing;vitrification achieves a higher recovery rate(P<0.05),motility(P<0.05),morphology(P<0.05),and curve line velocity(P<0.05)than slow ***,DNA fragmentation was decreased(P<0.05)and better acrosome protection(P<0.05)was exhibited in the spermatozoa after *** component analysis of all sperm parameters revealed that the vitrification cluster was closer to the fresh cluster,indicating that spermatozoa are better preserved through *** conclusion,while both slow freezing and vitrification have negative effects on sperm function and structure,the vitrification protocol described here had a relatively better recovery rate(65.8%)and showed improved preservation of several sperm quality parameters compared with slow freezing.

关键词： cryopreservation cryoprotectant slow freezing sperm vitrification

A STUDY ON STABILITY OF PENTADIENYL ANION ISOMERS

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Chinese Science Bulletin 1989年第4期34卷 348-349页

作者：王志中于恒泰杨忠志刘举正 Institute of Theoretical Chemistry Jilin University Changchun

Scientists have synthesized a number of organometallic compounds as substituents for pentadienyl anions. Studies on stability and electronic structures of pentadienyl anions （C5H7-） have been carried out from both experimental measurements and theoretical calculations. Up to now the conclusions are quite different. Among them Dewar has performed a complete geometrical optimization of C5H7- by MNDO method and pointed out that relative stability order of C5H7- isomers was W>S> *** a MONSTERGAUSS program we have performed STO-3G geometrical op-

关键词： isomers conclusions pointed favorable charges bonds listed diene shorter formal

Abnormal expression of osteocalcin on circulating endothelial progenitor cells in patients with ischemic cerebrovascular diseases

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Abnormal expression of osteocalcin on circulating endothelia...

影像的光芒与文化的悖论——首届中国电影伦理学高端论坛学术综述

第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Li liu zhi-zhong liu Guo-Jun Zhang Ya-Jie Wang Xi-Xiong Kang Center for Laboratory Diagnosis Beijing Tian Tan HospitalCapital Medical University

Background:Recent study implicated that endothelial progenitor cells(EPCs) in patients with coronary arteriosclerosis or severe aortic valve stenosis can express high level of osteocalcin. Because of different vascula... 详细信息

关键词： Osteocalcin Calcification Ischemic Cerebrovascular Disease Endothelial Progenitor Cells Angiostenosis

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艺术百家 2017年第4期33卷 254-256页

作者：袁智忠田毅刘好西南大学新闻传媒学院重庆400715

西南大学影视传播与道德教育研究中心与北京电影学院视觉文化批评研究中心共同发起并于2017年5月20日在重庆北碚联合举行了首届中国电影伦理学学术论坛，开启了中国电影理论界在电影伦理学这一维度的历史性航程。此次论坛由西南大学与... 详细信息

西南大学影视传播与道德教育研究中心与北京电影学院视觉文化批评研究中心共同发起并于2017年5月20日在重庆北碚联合举行了首届中国电影伦理学学术论坛，开启了中国电影理论界在电影伦理学这一维度的历史性航程。此次论坛由西南大学与北京电影学院联合主办、来自西南大学、北京电影学院、中国艺术研究院、中国电影家协会、北京师范大学、中国电影资料馆、中国文联电影艺术中心、中国传媒大学、陕西师范大学、四川大学、西南交通大学、西华大学、海南师范大学、重庆大学、重庆交通大学、重庆师范大学、重庆邮电大学、重庆工商大学、重庆长江师范学院、重庆市文化艺术研究中心、重庆市文化委电影处等30多个高校与研究机构70余名专家和学者参与了本次论坛。

关键词：中国电影学术论坛文化批评伦理学学术综述中国艺术研究院北京电影学院北京师范大学