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Apoptosis and its pathway in X gene-transfected HepG_2 cells

World Journal of Gastroenterology 2005年第28期11卷 4326-4331页

作者： Na Lin Hong-Ying Chen Dan Li Sheng-Jun Zhang zhi-xin cheng Xiao-Zhong Wang Department of Gastroenterology Affiliated Union Hospital Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To investigate the effect of hepatitis B virus （HBV） X gene on apoptosis and expressions of apoptosis factors in X gene-transfected HepG2 cells.METHODS： The HBV X gene eukaryon expression vector pcDNA3-X was transiently transfected into HepG2 cells by lipid-media transfection. Untransfected HepG2 and HepG2 transfected with pcDNA3 were used as controls. Expression of HBx in HepG2 was identified by RT-PCR. MTT and TUNEL were employed to measure proliferation and apoptosis of cells *** groups. Semi-quantified RT-PCR was used to evaluate the expression levels of Fas/FasL, Bax/Bcl-xL, and c-myc in each group.RESULTS： HBV X gene was transfected into HepG2 cells successfully. RT-PCR showed that HBx was only expressed in HepG2/pcDNA3-X cells, but not expressed in HepG2 and HepG2/pcDNA3 cells. Analyzed by MTT, cell proliferationcapacity was obviously lower in HepG2/pcDNA3-X cells （0.08910±0.003164） than in HepG2 （0.14410±0.004927） and HepG2/pcDNA3 cells （0.12150±0.007159）（P〈0.05 and P〈0.01）. Analyzed by TUNEL, cell apoptosis was much more in HepG2/pcDNA3-X cells （980/2 000） than HepG2 （420/2 000）, HepG2/pcDNA3 cells （520/2 000）（P〈0.05 and P〈0.01）. Evaluated by semi-quantified RT-PCR, the expression level of Fas/FasL was significantly higher in HepG2 cells transfected with HBx than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）. Bax/Bcl-xL expression level was also elevated in HepG2/pcDNA3-X cells （P〈0.05 and P〈0.01）. Expression of c-myc was markedly higher in HepG2/pcDNA3-X cells than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）.CONCLUSION： HBV X gene can impair cell proliferation capacity, improve cell apoptosis, and upregulate expression of apoptosis factors. The intervention of HBV X gene on the expression of apoptosis factors may be a possible mechanism responsible for the change in cell apoptosis and proliferation.

关键词： HBx Transfect HepG2 Apoptosis Fas FasL Bax Bcl-xL c-mvc

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论教育公平的法理基础

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云南民族大学学报（哲学社会科学版） 2008年第4期25卷 150-153页

作者：辛志成昆明冶金高等专科学校云南昆明650031

教育公平是社会公平的重要基础,它的实现对公民个人生存与发展尤其对社会进步、社会现代化的推动具有重大意义。从教育公平的概念、教育不公的现象及其产生原因进行剖析,分析教育公平的法理基础,旨在说明教育公平所体现的现代法治的价... 详细信息

教育公平是社会公平的重要基础,它的实现对公民个人生存与发展尤其对社会进步、社会现代化的推动具有重大意义。从教育公平的概念、教育不公的现象及其产生原因进行剖析,分析教育公平的法理基础,旨在说明教育公平所体现的现代法治的价值取向与法治的基本制度,以促进教育公平。

关键词：教育公平法治法理基础和谐社会

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牙线牵引辅助内镜黏膜下挖除术在治疗胃黏膜下肿瘤中的应用

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世界华人消化杂志 2022年第15期30卷 680-685页

作者：许捷鸿陈志新广西医科大学第四附属医院内镜诊疗室广西壮族自治区柳州市545027 广西医科大学第四附属医院消化内科广西壮族自治区柳州市545027

背景胃黏膜下肿瘤(submucosal tumor,SMT)往往侵及肌层,边界难以辨认,导致内镜下黏膜挖除术(endoscopic submucosal excision,ESE)操作难度增高,手术过程延长.牙线牵引辅助技术方法简便,能充分显露手术视野,降低手术难度,有效缩短手术... 详细信息

背景胃黏膜下肿瘤(submucosal tumor,SMT)往往侵及肌层,边界难以辨认,导致内镜下黏膜挖除术(endoscopic submucosal excision,ESE)操作难度增高,手术过程延长.牙线牵引辅助技术方法简便,能充分显露手术视野,降低手术难度,有效缩短手术操作时间.目的探讨牙线牵引辅助对内镜黏膜下挖除术在治疗胃黏膜下肿瘤的疗效及安全性.方法回顾性分析2017-01/2022-03在我院行内镜黏膜下挖除术(endoscopic submucosal excision,ESE)的胃黏膜下肿瘤患者75例,分为AB两组,A组行传统ESE术,共41例;B组行牙线牵引辅助ESE术,共34例;比较两组患者手术操作时间、术中黏膜下注射补充用量、术中出血量及术后迟发出血、发热等并发症.结果A组手术平均时间为(77.61±7.84)min、B组为(66.24±4.24)min;术中出血量A组为(13.51±10.25)mL,B组为(7.91±4.23)mL;术中黏膜下补充注射量A组为(44.85±4.75)mL、B组为(38.50±4.73)mL,2组手术平均时间、术中出血量及黏膜下补充注射量比较,差异有统计学意义(P0.05).术后A组患者迟发出血1例(2.4%),发热2例(4.9%);B组患者无迟发性出血,术后发热1例(2.9%)组间差异无统计学意义(P>0.05).结论牙线牵引辅助技术可为ESE治疗胃黏膜下肿瘤提供良好的手术视野,实现针对黏膜下肿瘤的精准剥离和挖除,缩短手术时间,从而一定程度上降低手术相关并发症发生风险.

关键词：胃黏膜下肿瘤内镜黏膜下挖除术牙线牵引辅助

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Zwitterionic hyaluronic acid derivatives for co-delivery of both chemotherapeutic and nucleic acid drugs in breast cancer treatment

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Nano Research 2025年第1期18卷 607-617页

作者： Ruo-Lin Jiang Hui-Na Liu Yu-Fan Yang zhi-cheng Zhang Qi Dai Xiao-Yan Bao Lin-Jie Wu Ya-xin Qin xin Tan Xiao-Yan Sun Xu-Fang Ying zhi-Qing Ben Min Han Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Radiation Oncology Key Laboratory of Cancer Prevention and InterventionThe Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310058China Hangzhou Institute of Innovative Medicine Zhejiang UniversityHangzhou 310058China Jinhua Institute of Zhejiang University Jinhua 321299China National Key Laboratory of Advanced Drug Delivery and Release Systems Zhejiang UniversityHangzhou 310058China Polytechnic institute Zhejiang UniversityHangzhou 310015China

Traditionally,hyaluronic acid has been widely used for drug delivery,but the current application bottleneck is that hyaluronic acid is hydrophilic and electronegative,which makes it difficult to carry hydrophobic drugs and small interfering RNA(siRNA)with the same *** on previous studies,we designed and synthesized hyaluronic acid nanocarriers HA-spermine/N,N,N-trimethylcystamine/DOX-TPP(HSTD)for loading siRNA to overcome the problem of siRNA release caused by strong electrostatic ***,N,N,Ntrimethylcystamine in the carrier can be degraded by intracellular glutathione to completely and rapidly release siRNA,thus promoting ***,when codelivered with the chemotherapy drug doxorubicin(DOX),this novel nanocarrier showed promising synergy in inhibiting tumor growth.

关键词： zwitterionic hyaluronic acid N N N-trimethylcystamine small interfering RNA(siRNA) doxorubicin

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A blood pressure measurement method based on synergetics theory

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Journal of Measurement Science and Instrumentation 2010年第S1期1卷 155-159,150页

作者： Hong-jin GUO,Jian-jun HAO,Ting WANG,zhi-xin cheng (College of Information and Electronical Engineering,Shandong University of Science and Technology,Qingdao 266510,China)

The principle for blood pressure measurement using pulse transit time is introduced in this *** the math model of synergetics theory is studied in *** synergetics theory is applied in the analysis of blood pressure measurement *** simulation results show that the application of synergetics theory is helpful to judge the normal blood pressure,and the accuracy is up to 80%.

关键词： pulse wave synergetics theor Blood pressure measurement

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Meta-analysis on clinical efficacy of Simiao Yong'an decoction in treatment of peripheral arterial occlusive diseases

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Journal of Hainan Medical University 2020年第16期26卷 62-68页

作者： Jin-Peng Jing Yue Zhang Yi Liu zhi-xin cheng Shandong University of Traditional Chinese Med Jinan250014China Affiliated hospital of Shandong University of Traditional Chinese Med Jinan250014China

Objective:To systematically evaluate the clinical efficacy and safety of Simiao Yong'an Decoction in the treatment of peripheral occlusive disease(PAOD).Methods:Eight randomized controlled clinical trials(RCT)of Simiao Yong'an Decoction in the treatment of PAOD were searched and screened from domestic and foreign databases(all from database construction to March 2020).The quality of the retrieved original studies was evaluated according to the evaluation criteria of Cochrane Handbook 5.1.0,and the included studies were meta-analyzed by RevMan5.3 ***:A total of 350 articles were retrieved,among which 14 studies met the inclusion criteria,with a total sample size of 1254 *** results of meta-analysis showed that:compared with conventional western Med,combined with Simiao Yong'an Decoction on the basis of western Med treatment can significantly improve the total clinical response rate of patients[RR=1.20,95%CI(1.14,1.27),P<0.00001],improve ankle brachial index(ABI)level[MD=0.79,95%CI(0.66,0.92),P<0.00001]and toe brachial index(TBI)level[RR=0.13,95%CI(0.10,0.16),P<0.00001],decreased c-reactive protein levels[MD=-8.55,95%CI(-8.99,-8.11),P<0.00001]and LDL levels[MD=-0.41,95%CI(-0.62,-0.19),P=0.0002],and increased HDL levels[MD=0.32(0.22,0.43),P<0.00001].There was no statistically significant difference in the incidence of adverse reactions[RR=0.50,95%CI(1.15,1.64),P=0.25].Conclusion:Simiao Yong'an Decoction combined with conventional western Med is more effective than conventional western Med in the treatment of ***,in view of the limitations of the quality of the analyzed literature,the positive results obtained in this study still need to be further verified by a large sample and multi-center clinical trial with a reliable research program.

关键词： Simiao Yong'an decoction Peripheral arterial occlusive disease Meta analysis

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星形胶质细胞条件培养液体外诱导胎鼠室管膜前下区神经干细胞向多巴胺能神经元的分化

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中国组织工程研究与临床康复 2009年第6期13卷 1040-1044页

作者：辛志成周政严稽文解放军第三军医大学新桥医院神经外科重庆市400037

背景:在神经干细胞移植治疗神经系统退行性变及修复神经系统功能损伤过程中,有效的神经干细胞体外增殖与多巴胺能神经元的定向诱导分化尤为关键。目的:以星形胶质细胞条件培养液为诱导剂,观察胎鼠室管膜前下区神经干细胞体外向多巴胺能... 详细信息

背景:在神经干细胞移植治疗神经系统退行性变及修复神经系统功能损伤过程中,有效的神经干细胞体外增殖与多巴胺能神经元的定向诱导分化尤为关键。目的:以星形胶质细胞条件培养液为诱导剂,观察胎鼠室管膜前下区神经干细胞体外向多巴胺能神经元的分化。设计、时间及地点:细胞学体外对照观察,于2006-12/2007-08在解放军第三军医大学新桥医院实验中心完成。材料:清洁级新生2d龄KM小鼠15只,孕16dWistar大鼠40只,均由解放军第三军医大学实验动物中心提供。方法:采用差速黏附法和振荡分离法纯化培养KM小鼠星形胶质细胞,收集传至第3代、培养5d的星形胶质细胞条件培养液,-20℃冻存待用。体外分离Wistar胎鼠室管膜前下区神经干细胞,加入含B27和碱性成纤维细胞生长因子的DMEM/F12无血清培养基进行原代培养,传至第3代后按0.5×108L-1密度接种,设立2组:对照组单纯加入含体积分数0.1为胎牛血清的DMEM/F12培养基予以自然分化,实验组加入已制备的星形胶质细胞条件培养液进行诱导分化。主要观察指标:免疫细胞化学染色鉴定胎鼠室管膜前下区神经干细胞,流式细胞仪检测胎鼠室管膜前下区神经干细胞向多巴胺能神经元分化的阳性率。结果:培养的神经球细胞表达巢蛋白,可分化为神经元特异性烯醇化酶、胶质纤维酸性蛋白阳性细胞。诱导分化7d后,实验组可见酪氨酸羟化酶阳性细胞,胞体呈圆形或椭圆形,酪氨酸羟化酶位于胞质及突起中;对照组酪氨酸羟化酶阳性细胞在数量、细胞成熟形态上均未达到实验组水平。实验组酪氨酸羟化酶阳性细胞分化率明显高于对照组(t=35.296,P<0.01)。结论:在体外星形胶质细胞条件培养液可显著促进胎鼠室管膜前下区神经干细胞向多巴胺能神经元分化。

关键词：神经干细胞星形胶质细胞条件培养液多巴胺能神经元

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关于高职高专院校应用文写作课的教学思考

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昆明冶金高等专科学校学报 2007年第4期23卷 36-40页

作者：辛志成昆明冶金高等专科学校社会科学与公共学院云南昆明650033

"应用文写作"是高职高专院校面向全校学生所开设的一门应用性和技能性的公共基础课。目前应用文写作教学从观念转变到课程设置、教学体系的规范到教学方式方法的改革与创新,都存在着许多较为突出且亟待解决的问题和误区;通过对应用文写... 详细信息

"应用文写作"是高职高专院校面向全校学生所开设的一门应用性和技能性的公共基础课。目前应用文写作教学从观念转变到课程设置、教学体系的规范到教学方式方法的改革与创新,都存在着许多较为突出且亟待解决的问题和误区;通过对应用文写作教学活动全过程三个主要环节的分析,结合传统与现代、他人观点与自身感受,试图在如何创新教学方法上有所突破。

关键词：高职高专应用文写作教学方法

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基于ATM单瓶颈节点模型的PID拥塞控制器设计

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计算机科学 2004年第1期31卷 53-55,65页

作者：关新平刘志新龙承念燕山大学电气工程学院秦皇岛066004

针对ATM单瓶颈节点网络模型,提出ABR业务拥塞控制方案,给出使系统闭环稳定PID参数应满足的约束条件。设置前馈环节以抑制带宽波动对缓冲区队列长度的影响。仿真结果表明,业务服务质量得到了保证,系统具有较好的动态性能。

关键词： PID拥塞控制器 ATM 动态性能缓冲区通信网络反馈机制

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Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： Wen Gao Jing-Wen Li Hui Ye Xue-zhi Zhang Jian-Xiang Liu Hong cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

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