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Integrative transcriptome,proteome,and microRNA analysis reveals the effects of nitrogen sufficiency and deficiency conditions on theanine metabolism in the tea plant(Camellia sinensis)

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Horticulture Research 2020年第1期7卷 1865-1877页

作者： zhi-wei liu Hui Li Jie-Xia liu Yu Wang Jing Zhuang Tea Science Research Institute Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University210095 NanjingChina State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University210095 NanjingChina

Nitrogen(N)is associated with amino acid metabolism in higher *** is an important amino acid in tea *** explore the relationship between theanine metabolism and N conditions,we examined the differentially expressed genes(DEGs),proteins(DEPs),and microRNAs(DEMs)involved in theanine metabolism in tea plant shoots and roots under N sufficiency and deficiency ***,proteome,and microRNA analyses were performed on tea plant shoots and roots under N sufficiency and deficiency *** contents of theanine,expression levels of genes involved in theanine metabolism,contents of proteinogenic amino acids,and activity of enzymes were *** DEP–DEG correlation pairs and negative DEM–DEG interactions related to theanine metabolism were identified based on correlation *** expression profiles of DEGs and negative DEM–DEG pairs related to theanine biosynthesis were consistent with the sequencing *** results suggest that the molecular and physiological mechanism of theanine accumulation is significantly affected by N sufficiency and deficiency *** DEGs,DEPs,and DEMs and the activity of the enzymes involved in theanine biosynthesis might play vital roles in theanine accumulation under N sufficiency and deficiency conditions in the shoots and roots of tea plants.

关键词： metabolism sinensis ani

Remediation of Ni^(2+)-contaminated water using iron powder and steel manufacturing byproducts

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Journal of Environmental Sciences 2006年第3期18卷 464-467页

作者： JIN Jian ZHAO wei-rong XU Xin-hua HAO zhi-wei liu Yong HE Ping ZHOU Mi Department of Environment Engineering Zhejiang University Hangzhou 310027 China

Steel manufacturing byproducts and commercial iron powders were tested in the treatment of Ni^2＋-contaminated water. Ni^2＋ is a priority pollutant of some soils and groundwater. The use of zero-valent iron, which can reduce Ni^2＋ to its neural form appears to be an alternative approach for the remediation of Ni^2＋-contaminated sites. Our experimental data show that the removal efficiencies of Ni^2＋ were 95.15% and 94.68% at a metal to solution ratio of 20 g/L for commercial iron powders and the steel manufacturing byproducts in 60 min at room temperature, respectively. The removal efficiency reached 98.20% when the metal to solution ratio was 40 g/L for commercial iron powders. Furthermore, we found that the removal efficiency was also largely affected by other factors such as the pHs of the treated water, the length of time for the metal to be in contact with the Ni^2＋-contaminated water, initial concentrations of metal solutions, particle sizes and the amount of iron powders. Surprisingly, the reaction temperature appeared to have little effect on the removal efficiency. Our study opens the way to further optimize the reaction conditions of in situ remediation of Ni^2＋ or other heavy metals on contaminated sites.

关键词： zero-valent iron reduction remediation Ni^2+

^(3)S_(1)–^(3)D_(1) coupled channel Λ_(c)N interactions:chiral effective field theory versus lattice QCD

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Communications in Theoretical Physics 2023年第1期75卷 86-95页

作者： Jing Song Yang Xiao zhi-wei liu Kai-Wen Li Li-Sheng Geng School of space and environment Beihang UniversityBeijing102206China School of Physics Beihang UniversityBeijing102206China UniversitéParis-Saclay CNRS/IN2P3IJCLabOrsayF-91405France Medical Management Department CAS Ion Medical Technology Co.LtdBeijing 100190China Beijing Advanced Innovation Center for Big Data-Based Precision Medicine School of Medicine and EngineeringBeihang UniversityKey Laboratory of Big Data-Based Precision Medicine(Beihang University)Ministry of Industry and Information TechnologyBeijing100191China Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang UniversityBeijing102206China School of Physics and Microelectronics Zhengzhou UniversityZhengzhouHenan450001China

We study the lattice QCDΛ_(c)N phase shifts for the^(3)S_(1)-^(3)D_(1)coupled channel using both the leading order covariant chiral effective theory and the next-to-leading order non-relativistic chiral effective field theory(ChEFT).We show that although it is possible to describe simultaneously the^(3)S_(1)and^(3)D_(1)phase shifts and the inelasticityη_(1),the fitted energy range is quite small,only up to E_(c.m.)=5 *** raises concerns regarding the consistency between leading/next-to-leading order ChEFT and the lattice QCD simulations.

关键词： Λ_(c)N interaction covariant ChEFT lattice QCD

Differentially expressed protein and gene analysis revealed the effects of temperature on changes in ascorbic acid metabolism in harvested tea leaves

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Horticulture Research 2018年第1期5卷 263-275页

作者： Hui Li zhi-wei liu zhi-Jun Wu Yong-Xin Wang Rui-Min Teng Jing Zhuang Tea Science Research Institute College of HorticultureNanjing Agricultural UniversityNanjing210095China

Tea is an important non-alcoholic beverage *** quality is determined by numerous secondary metabolites in harvested tea leaves,including tea polyphenols,theanine,caffeine,and ascorbic acid(AsA).AsA metabolism in harvested tea leaves is affected by storage and transportation ***,the molecular mechanisms underlying AsA metabolism in harvested tea leaves exposed to different storage and transportation temperature conditions remain *** we performed RP-HPLC to detect dynamic changes in AsA content in tea leaves subjected to high-(38°C),low-(4°C),or room-temperature(25°C)*** AsA distribution and levels in the treated tea leaves were analyzed using cytological–anatomical characterization *** differentially expressed CsAPX1 and CsDHAR2 proteins,which are involved in the AsA recycling pathway,were identified from the corresponding proteomic data using *** also analyzed the expression profiles of 18 genes involved in AsA metabolism,including CsAPX1 and *** was mainly distributed in tea leaf mesophyll ***-and low-temperature treatments upregulated the CsAPX1 and CsDHAR2 proteins and induced CsAPX and CsDHAR2 gene *** results indicated that the CsAPX1 and CsDHAR2 proteins might have critical roles in AsA recycling in tea *** results provide a foundation for the in-depth investigation of AsA metabolism in tea leaves during storage and transportation,and they will promote better tea flavor in tea production.

关键词： metabolism expression. leaves

Palladium-catalyzed highly regioselective Heck reaction of aryl nonaflates with electron-rich olefins

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Chinese Chemical Letters 2008年第9期19卷 1017-1020页

作者： Dan Xu zhi Hua liu wei Jun Tang Jun Mo Li Jin Xu Department of Chemistry Renmin University of China Beijing 100872 China

Highly efficient palladium-catalyzed Heck coupling of aryl nonaflates with electron-rich vinyl ethers, and enamides is described. These reactions afforded exclusively the branched products in good yields. The results ... 详细信息

关键词： Aryl nonaflates Electron-rich Palladium Heck reaction Regioselectivity

Thermal Properties of Mg–Al/AlN Composites Fabricated by Powder Metallurgy

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Acta Metallurgica Sinica(English Letters) 2018年第6期31卷 641-649页

作者： Jie Chen Chong-Gao Bao zhi-wei liu Ben-Shuang Sun Yong-Chun Shu Qing-Kui Li Henan Province Industrial Technology Research Institute of Resources and Materials Zhengzhou University State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and EngineeringXi’an Jiaotong University

Magnesium matrix composites reinforced with AlN particles were fabricated by the powder metallurgy *** mixing methods were used in this study to control the distribution of Al N *** microstructure,thermal diffusivity and thermal expansion of the Mg–Al/Al N composites using different mixing methods were *** results showed that the intergranular and intragranular distributions of Al N particles were obtained,respectively,by controlling the mixing *** composite with intragranular particles exhibited lower thermal diffusivity because of the existences of more interfaces,defects and grain boundaries,which acted as scattering centers and reduced the mean free path of electrons and *** existence of Al N particles lowered the coefficient of thermal expansion（CTE）and enhanced the dimensional stability of the *** the use of the improved mixing method further reduced the CTE of Mg–Al/Al N composites.

关键词： Magnesium matrix composite Microstructure Thermal diffusivity Thermal expansion Mixing

Oxygen isotopic ratios toward molecular clouds in the Galactic disk

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Research in Astronomy and Astrophysics 2016年第3期16卷 99-106页

作者： Hai-Kun Li Jiang-Shui Zhang zhi-wei liu Deng-Rong Lu Min Wang Jin Wang Center for Astrophysics Guangzhou University School of Physics and Mechanical & Electrical Engineering Xiamen University Purple Mountain Observatory Qinghai Station Purple Mountain Observatory Chinese Academy of Sciences

We present our observations of the J = 1-0 rotation transitions in molecular isotopes C^18O and C^17O toward a sample of molecular clouds with different galactocentric distances, using the Delingha 13.7 m（DLH 13.7 m） telescope, administered by Purple Mountain Observatory, and its 9-beam SIS *** observations toward several sources with large galactocentric distance are obtained with the IRAM 30 m and Mopra 22 m telescopes. C^18O/C^17O abundance ratios reflecting the ^（18）O/^（17）O isotope ratios are obtained from integrated intensity ratios of C^18O and C^17O. We derived the ratio value for 13 sources covering a galactocentric distance range of 3 kpc to 16 kpc. In combination with our mapping results that provide a ratio value of 3.01±0.14 in the Galactic center region, it shows that the abundance ratio tends to increase with galactocentric distance, i.e., it supports a radial gradient along the Galactic disk for the abundance ratio. This is consistent with the inside-out formation scenario of our Galaxy. However, our results may suffer from small samples with large galactocentric distance. Combining our data with multitransition lines of C^18O and C^17O will be helpful for constraining opacities and abundances and further confirming the Galactic radial gradient shown by the isotope ratio ^（18）O/^（17）O.

关键词： ISM: abundances -- ISM: clouds -- ISM: molecules -- Galaxy: abundances -- radio lines:ISM

Blood compatibility of zinc-calcium phosphate conversion coating on Mg-1.33Li-O.6Ca alloy

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Frontiers of Materials Science 2016年第3期10卷 281-289页

作者： Yu-Hong ZOU Rong-Chang ZENG Qing-Zhao WANG Li-Jun liu Qian-Qian XU Chuang WANG zhi-wei liu College of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao 266590 China College of Materials Science and Engineering Shandong University of Science and Technology Qingdao 266590 China Hospital of Shandong University of Science and Technology Qingdao 266590 China State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology Shandong University of Science and Technology Qingdao 266590 China

Magnesium alloys as a new class of biomaterials possess biodegradability and biocompatibility in comparison with currently used metal implants. However, their rapid corrosion rates are necessary to be manipulated by appropriate coatings. In this paper, a new attempt was used to develop a zinc-calcium phosphate （Zn-Ca-P） conversion coating on Mg-1.33Li-0.6Ca alloys to increase the biocompatibility and improve the corrosion resistance. In vitro blood biocompatibility of the alloy with and without the Zn-Ca-P coating was investigated to determine its suitability as a degradable medical biomaterial. Blood biocompatibility was assessed from the hemolysis test, the dynamic cruor time test, blood cell count and SEM observation of the platelet adhesion to membrane surface. The results showed that the Zn-Ca-P coating on Mg-1.33Li-0.6Ca alloys had good blood compatibility, which is in accordance with the requirements for medical biomaterials.

关键词： magnesium alloy lithium biomaterial biocompatibility zinc-calciumphosphate coating

Left hepatectomy with caudate lobe resection using the methylene blue staining technique for bismuth lllb hilar cholangiocarcinoma

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Hepatobiliary Surgery and Nutrition 2019年第6期8卷 674-676页

作者： Ji-Ye Chen Shou-Wang Cai zhi-wei liu Xian-Lei Xing Hospital and Institute of Hepatobiliary Surgery Chinese PLA General HospitalChinese PLA Postgraduate Medical SchoolBeijing 100853China

Left hepatectomy with caudate lobe resection has been widely accepted as the standard treatment for bismuth IIIb hilar cholangiocarcinoma(1-6).However,there is no clear boundary between the right caudate lobe and the right posterior *** methylene blue staining technique are commonly used to delineate the margin of the relevant hepatic segment for anatomic hepatectomy(7).Herein,we described this technique to demarcate the boundary between the caudate lobe and the right posterior lobe for an en bloc resection of the tumor and improve the R0 surgical margin.

关键词： resection hepatectomy hilar