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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 667-672页

作者： Tian-Hong WANG*, Ti liU, zhi-Hong WU, shi-li liU, Yi LU, and Yin-Bo QU The State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China The State Key Laboratory of Microbial Technology Shandong UniversityJinan 250100 China

To construct strains of the filamentous fungus Trichoderma reesei with low cellobiohydrolases while high endoglucanase activity, the Pcbh1-eg3-Tcbh1 cassette was constructed and the coding sequence of the cellobiohydrolase I (CBHI) gene was replaced with the coding sequence of the eg3 gene by homologous recombination. Disruption of the cbh1 gene was confirmed by PCR, Southern dot blot and Western hybri- dization analysis in two transformants denoted as L13 and L29. The filter paper-hydrolyzing activity of strain L29 was 60% of the parent strain Rut C30, and the CMCase activity was increased by 33%. This relatively modest increase suggested that the eg3 cDNA under the control of the cbh1 promoter was not efficiently transcribed as the wild type cbhl gene. However our results confirmed that homologous recombination could be used to construct strains of the filamentous fungus Trichoderma reesei with novel cellulase profile. Such strains are of interest from the basic science perspective and also have potential industrial applications.

关键词： Trichoderma reesei gene replacement gene disruption cbh1 eg3

Risk factors for Enterobius vermicularis infection in children in Gaozhou, Guangdong, China

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Infectious Diseases of Poverty 2015年第1期4卷 220-226,219页

作者： Hong-Mei li Chang-Hai Zhou zhi-shi li Zhuo-Hui Deng Cai-Wen Ruan Qi-Ming Zhang Ting-Jun Zhu Long-Qi Xu Ying-Dan Chen National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionWHO Collaborative Center for MalariaSchistosomiasis and FilariasisKey Laboratory of Parasite and Vector BiologyMinistry of HealthShanghai 200025China Gaozhou Center for Disease Control and Prevention Gaozhou 515200China Guangdong Center for Disease Control and Prevention Guangzhou 510000China

Background:Enterobius vermicularis infection is a prevalent intestinal parasitic disease in *** this study,we explored the epidemiological status and risk factors for *** infection in children in southern ***:A cross-sectional survey was carried out in Gaozhou city,Guangdong province,China,in December *** aged 2–12 years from five schools participated in the *** adhesive cellophane-tape perianal swab method was applied to detect *** infection,while a questionnaire was sent to each child’s guardian(s)to collect demographic and socioeconomic data,as well as hygiene behaviors,pertaining to each *** and multivariate logistic regression analyses were performed to capture the potential risk ***:Out of the 802 children surveyed,440 were infected with ***,with an average prevalence of 54.86%,and a range from 45.96 to 68.13%.The age variable was found to be statistically significant,whereas the sex variable was *** was found that a mother’s education level(low)and not washing hands before dinner were major risk factors in all children(802).After stratification by age,a father’s education level(primary or below)and biting pencils(or toys)were significant risk factors in the younger children(508),while not washing hands before dinner and playing on the ground were important risk factors in the older children(294).Conclusion:This study demonstrates the prevalence of *** infection in children in Gaozhou and reveals underlying risk *** importantly,it reveals that risk factors differ among the different age groups,which indicates that different control measures targeted at particular age groups should be implemented.

关键词： Enterobius vermicularis Prevalence Risk factor Children China

Joint Feature Encoding and Task Alignment Mechanism for Emotion-Cause Pair Extraction

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Computers, Materials & Continua 2025年第1期82卷 1069-1086页

作者： shi li Didi Sun College of Computer and Control Engineering Northeast Forestry UniversityHarbin150040China

With the rapid expansion of social media,analyzing emotions and their causes in texts has gained significant ***-cause pair extraction enables the identification of causal relationships between emotions and their triggers within a text,facilitating a deeper understanding of expressed sentiments and their underlying *** comprehension is crucial for making informed strategic decisions in various business and societal ***,recent research approaches employing multi-task learning frameworks for modeling often face challenges such as the inability to simultaneouslymodel extracted features and their interactions,or inconsistencies in label prediction between emotion-cause pair extraction and independent assistant tasks like emotion and cause *** address these issues,this study proposes an emotion-cause pair extraction methodology that incorporates joint feature encoding and task alignment *** model consists of two primary components:First,joint feature encoding simultaneously generates features for emotion-cause pairs and clauses,enhancing feature interactions between emotion clauses,cause clauses,and emotion-cause ***,the task alignment technique is applied to reduce the labeling distance between emotion-cause pair extraction and the two assistant tasks,capturing deep semantic information interactions among *** proposed method is evaluated on a Chinese benchmark corpus using 10-fold cross-validation,assessing key performance metrics such as precision,recall,and F1 *** results demonstrate that the model achieves an F1 score of 76.05%,surpassing the state-of-the-art by 1.03%.The proposed model exhibits significant improvements in emotion-cause pair extraction(ECPE)and cause extraction(CE)compared to existing methods,validating its *** research introduces a novel approach based on joint feature encoding and task alignment mechanisms,contributing to advancements in emotion-c

关键词： Emotion-cause pair extraction interactive information enhancement joint feature encoding label consistency task alignment mechanisms

Interpretable Machine Learning Method for Compressive Strength Prediction and Analysis of Pure Fly Ash-based Geopolymer Concrete

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 65-78页

作者： shi Yuqiong li Jingyi ZHANG Yang li li Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of Ministry of Education Northwest A&F Uni-versityYangling 712100China College of Water Resources and Architectural Engineering Northwest A&F UniversityYangling 712100China Chongqing Key Laboratory of Public Big Data Security Technology Chongqing 401420China Chongqing College of Mobile Communication Chongqing 401520China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina National Rail Transit Electrification and Automation Engineering Technology Research Center(Hong Kong Branch) The Hong Kong Polytechnic UniversityKowloonHung HomHong KongChina

In order to study the characteristics of pure fly ash-based geopolymer concrete(PFGC)conveniently,we used a machine learning method that can quantify the perception of characteristics to predict its compressive *** this study,505 groups of data were collected,and a new database of compressive strength of PFGC was *** order to establish an accurate prediction model of compressive strength,five different types of machine learning networks were used for comparative *** five machine learning models all showed good compressive strength prediction performance on *** them,R2,MSE,RMSE and MAE of decision tree model(DT)are 0.99,1.58,1.25,and 0.25,*** R2,MSE,RMSE and MAE of random forest model(RF)are 0.97,5.17,2.27 and 1.38,*** two models have high prediction accuracy and outstanding generalization *** order to enhance the interpretability of model decision-making,we used importance ranking to obtain the perception of machine learning model to 13 *** 13 variables include chemical composition of fly ash(SiO_(2)/Al_(2)O_(3),Si/Al),the ratio of alkaline liquid to the binder,curing temperature,curing durations inside oven,fly ash dosage,fine aggregate dosage,coarse aggregate dosage,extra water dosage and sodium hydroxide *** temperature,specimen ages and curing durations inside oven have the greatest influence on the prediction results,indicating that curing conditions have more prominent influence on the compressive strength of PFGC than ordinary Portland cement *** importance of curing conditions of PFGC even exceeds that of the concrete mix proportion,due to the low reactivity of pure fly ash.

关键词： machine learning pure fly ash geopolymer compressive strength feature perception

K-H Instability of Dust Plasma

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紫金山天文台台刊 1996年第2期15卷 84-88页

作者： shi zhi-dong and li Zhong-yuanUniversity of Science and Technology of China,Hefei,230026,PRC Advanced Centre for Earth Science and Astronomy,Third World Academy of Science

Areview about K-H instability related to comet tail is *** effect of dust graing on KHI is theoretically analysed and relations of critical shear to excit KHI with the properties of dust grains both for sheared ion fl... 详细信息

关键词： K-H instability comet tail dust plasma.

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RNAi及其在肿瘤研究中的应用

生物化学与生物物理进展 2004年第6期31卷 492-499页

作者：石智符立梧中山大学肿瘤防治中心广州510060

RNA干扰 (RNAinterference ,RNAi)是指在生物体细胞内 ,外源性或内源性的双链RNA (double strandedRNA ,dsRNA)引起与其同源mRNA特异性的降解 ,因而抑制其相应的基因表达过程 .由于它能够高度特异性、高效性地抑制基因的表达 ,因此在研... 详细信息

RNA干扰 (RNAinterference ,RNAi)是指在生物体细胞内 ,外源性或内源性的双链RNA (double strandedRNA ,dsRNA)引起与其同源mRNA特异性的降解 ,因而抑制其相应的基因表达过程 .由于它能够高度特异性、高效性地抑制基因的表达 ,因此在研究基因功能及表达调控、信号传导通路、药物靶点的鉴定和基因药物开发等方面具有良好的应用前景 .主要介绍RNAi可能的分子机制、分子生物学特性。

关键词： RNAi siRNA 基因抑制肿瘤

Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent liCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie shi Changle Hou Jingjing Dong Dong Chen Jianzhong li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(liBs),properly recycling spent liBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of liB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to liCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/liCoO_(2)mass ratio of 3:1,the leaching efficiency of li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in liCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of liB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang Qing shi Guofan Zhang Yuxuan Zhu Binbin li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

CHARGING OF DUST GRAINS IN COMETARY PLASMA ENVIRONMENTS

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紫金山天文台台刊 1998年第2期 92-96页

作者： shi zhi-dong li Zhong-yuan( University of science and Technology of China, Hefei, 230026, P. R , C . ) (Advanced center for Earht Science and Astronomy, Third World Academy of Science)

The equilibrium potential of fluffy dust grains in plasma environments is modeled numerically for comet P/Halley. It is found that the dust grain acquires its largest negative potential and probability of disruption a... 详细信息

关键词： comets - plasma - dust - charging

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泵站沉井刃脚结构形式设计的探讨

中国给水排水 2013年第24期29卷 57-60页

作者：陈平史志利天津市市政工程设计研究院天津300051

结合市政泵站工程实例,对沉井应用于泵站工程中时,刃脚结构形式对壁板内力的影响进行了探讨和研究,为类似工程的设计提供了参考依据。

关键词：沉井泵站刃脚