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Chemical Research in Chinese Universities 2006年第3期22卷 343-346页

作者： XU Qing-xuan YUE Hao LIU zhi-qiang WANG Yong yan Cun-yu LIU Shu-ying Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China

Electrospray ionization mass spectrometry（ESI-MS） was applied simultaneously in determining norditerpenoid alkaloids from the roots of Aconitum sinomantanum Nakai （RAS） based on molecular mass information. The tandem mass spectra（ ESI-MS^n） provided the alkaloidal structural information, through which the existence of these alkaloids was further confirmed. Accordingly, six known norditerpenoid alkaloids were simultaneously determined on the basis of their ESI-MS^n spectra. Furthermore, based on the diagnostic fragmentation pathways of alkaloidal MS^n, a rapid method for direct detection and characterization of alkaloids from an ethanolic extract of RAS was described.

关键词： Electrospray ionization tandem mass spectrometry( ESI-MS^n) Norditerpenoid alkaloid Aconitum sinomantanum Nakai

Adhesion behavior of endothelial progenitor cells to endothelial cells in simple shear flow

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Acta Mechanica Sinica 2011年第6期27卷 1071-1080页

作者： Xiao-Bo Gong Yu-Qing Li Quan-Chao Gao Bin-Bin Cheng Bao-Rong Shen zhi-qiang yan Zong-Lai Jiang Institute of Mechanobiology and Biomedical Engineering School of Life Sciences and Biotechnology Shanghai Jiao Tong University 200240 Shanghai China Department of Engineering Mechanics School of Naval Architecture Ocean and Civil Engineering Shanghai Jiao Tong University 200240 Shanghai China

The adhesion of endothelial progenitor cells（EPCs） on endothelial cells（ECs） is one of the critical physiological processes for the regenesis of vascular vessels and the prevention of serious cardiovascular ***,the rolling and adhesion behavior of EPCs on ECs was studied numerically.A two-dimensional numerical model was developed based on the immersed boundary method for simulating the rolling and adhesion of cells in a channel *** binding force arising from the catch bond of a receptor and ligand pair was modeled with stochastic Monte Carlo method and Hookean spring *** effect of tumor necrosis factor alpha（TNF-α） on the expression of the number of adhesion molecules in ECs was analyzed experimentally.A flow chamber system with CCD camera was set up to observe the top view of the rolling of EPCs on the substrate cultivated with *** results prove that the adhesion of EPC on ECs is closely related to membrane stiff-ness of the cell and shear rate of the *** also suggests that the adhesion force between EPC and EC by P-selectin glycoprotein ligand-1 only is not strong enough to bond the cell onto vessel walls unless contributions of other catch bond are *** results demonstrate that TNF-α enhanced the expressions of VCAM,ICAM,P-selectin and E-selectin in ECs,which supports the numerical results that the rolling velocity of EPC on TNF-α treated EC substrate decreases obviously compared with its velocity on the untreated *** is found that because the adhesion is affected by both the rolling velocity and the deformability of the cell,an optimal stiffness of EPC may exist at a given shear rate of flow for achieving maximum adhesion rates.

关键词： Endothelial progenitor cell Endothelial cell Cell adhesion. Flow chamber. Immersed boundary method

Enhanced head-to-head photodimers in the photocyclodimerization of anthracenecarboxylic acid with a cationic pillar[6]arene

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Chinese Chemical Letters 2016年第7期27卷 1017-1021页

作者： Jian-Chang Gui zhi-qiang yan Yuan Peng Ji-Gao Yi Da-yang Zhou Dan Su zhi-Hui Zhong Guo-Wei Gao Wan-Hua Wu Cheng yang Key Laboratory of Green Chemistry & Technology College of Chemistry State Key Laboratory of Biotherapy West China Medical Center and State Key Laboratory of Polymer Materials Engineering Sichuan University Chengdu 610064 China Chengdu Environmental Monitoring Center Chengdu 610042 China Comprehensive Analysis Center ISIR Osaka University 8-I Mihogaoka lbaraki Osaka 5670047 Japan

The complexation behaviors of anthracenecarboxylic acid and water-soluble cationic pillararenes have been investigated by 1H NMR,UV-vis and ITC *** cationic pillar[6]arene was found to stepwise form 1：1 and 1：2 complexes,having a large K1 and a relatively small K2 *** of AC within the pillar[6]arene improved the yield of the head-to-head *** to 4.97 HH/HT ratio has been reached by optimizing the reaction conditions.

关键词： Pillararene Photocyclodimerization Anthracenecarboxylic acid Supramolecular complexation

Characteristics and Advantages of Regional Grain Production in Guangxi Zhuang Autonomous Region of China

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Asian Agricultural Research 2010年第9期2卷 1-4,9页

作者： ZHANG Fei, yan zhi-qiang, WEI yan-fei College of Resources and Environmental Sciences, Guangxi Teachers Education University, Nanning 530001, China

According to the latest Land Use Planning of Guangxi Zhuang Autonomous Region, Guangxi is divided into five regions, which are eastern Guangxi, western Guangxi, southern Guangxi, northern Guangxi, and central Guangxi. Regional variation characteristics of grain production are introduced from two aspects of the decline of grain sowing area and the increase of grain output. According to the data in Guangxi Statistical Yearbook, comparative advantages of regional grain production in Guangxi Zhuang Autonomous Region are analyzed by the method of Comprehensive Comparative Dominance Index. Result shows that eastern Guangxi and northern Guangxi have the maximum comprehensive dominance indices with the minimum increasing amount of dominance indices. Their advantages in grain production are gradually diminished; and the grain production center is moving to central Guangxi and southern Guangxi. In order to solve the problems in grain production, Guangxi should make full use of the comparative advantage in agricultural production, carry out structural adjustment of agricultural production, and try to realize the rational distribution and specialization of agricultural production.

关键词： Grain production Comparative advantage Dominance i

抗肿瘤血管抗体AA98功能片段V_H/κ的脲浓度梯度柱复性

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生物化学与生物物理进展 2002年第4期29卷 645-650页

作者：张志强林芸阎锡蕴中国科学院微生物研究所

利用稀释法、凝胶过滤、脲浓度梯度凝胶过滤 3种方法研究了抗肿瘤血管抗体功能片段VH/κ的体外复性 .通过考察复性液中还原型谷胱甘肽 /氧化型谷胱甘肽 (GSH/GSSG)比例、精氨酸浓度、pH值、洗脱速度、变性液中蛋白质浓度、凝胶过滤脲梯... 详细信息

利用稀释法、凝胶过滤、脲浓度梯度凝胶过滤 3种方法研究了抗肿瘤血管抗体功能片段VH/κ的体外复性 .通过考察复性液中还原型谷胱甘肽 /氧化型谷胱甘肽 (GSH/GSSG)比例、精氨酸浓度、pH值、洗脱速度、变性液中蛋白质浓度、凝胶过滤脲梯度长度等因素 ,发展了脲梯度凝胶过滤柱复性VH/κ的方法 .结果表明 ,与传统的稀释法和柱复性法相比 ,脲梯度法复性获得的VH/κ的活性回收率和相对亲和力均有显著提高 .

关键词：抗肿瘤血管抗体AA98 功能片段 VH/κ 脲浓度梯度柱复性柱复性单链抗体基因工程抗体

Optimization of the Acoustic Pressure Distribution in a Resonant Photoacoustic Cell

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Chinese Journal of Lasers 2001年第2期 28-34页

作者： HAO Luyuan REN zhi SHI qiang ZHENG yan ZHENG Jingjing ZHU Qingshi (Open Laboratory of Bond Selective Chemistry,University of Science and Technology of China, Hefei 230026, China)

The size of a cylindrical photoacoustic cell with suitable size was selected so that the resonant frequency of the first radial mode was equal to that of a longitudinal higher mode. By maintaining two thin coaxial tubes at each end, a enhanced photoacoustic cell was constructed with two tubes of 1/2 and 1/4 of the wavelength. In this enhanced photoacoustic cell, both the first radial resonance and the higher longitudinal resonance were excited adequately. Coupling of two acoustic modes makes the acoustic energy concentrating in the middle of the cell. The surface loss was decreased,the acoustic quality factor and pressure amplitude increased obviously as compared with conventional cylindrical cell.

关键词： photoacoustic spectrum acoustic mode resonant coupl

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基于偏振特性对石英玻璃和绿漆涂层的反演

中国光学 2016年第5期9卷 547-553页

作者：苏志强颜昌翔张军强杨斌中国科学院长春光学精密机械与物理研究所吉林长春130033 中国科学院大学北京100049

物质的偏振特性与其复折射率、表面粗糙度以及观测几何条件有关,为了应用偏振探测技术实现对目标的定量反演,本文首先对两种典型目标(绿漆涂层和石英玻璃)的偏振特性进行了实验测定,并对偏振度与探测天顶角的关系进行了分析。利用实验... 详细信息

物质的偏振特性与其复折射率、表面粗糙度以及观测几何条件有关,为了应用偏振探测技术实现对目标的定量反演,本文首先对两种典型目标(绿漆涂层和石英玻璃)的偏振特性进行了实验测定,并对偏振度与探测天顶角的关系进行了分析。利用实验数据并基于描述目标偏振特性的PG模型首次在考虑了粗糙度的影响下,对目标的折射率、消光系数进行了定量反演,最后将反演结果与参考结果进行比较。结果表明,石英玻璃的折射率相对误差为4.944 9%,绿漆涂层的折射率与消光系数的相对误差分别为11%和21.558 9%。该方法在考虑表面粗糙度的条件下能够更精确地测定物质的复折射率,同时也为偏振技术应用于目标定量反演提供了依据。

关键词：偏振遥感目标识别复折射率粗糙度

The role of Dby mRNA in early development of male mouse zygotes

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Asian Journal of Andrology 2010年第4期12卷 567-577页

作者： Chen-Jiang Yao Wang-Jie Xu Xiu-Li Gong Ying Zhou zhi-qiang yan Zi-Jue Zhu Zhao-Xia Wang Qiao-Li Li Xin-Bin Guo Lian-Yun Wang Duan Ma Zhong-Dong Qiao Department of Biological Science and Technology Key Laboratory of Developmental Genetics ＆ Neuropsychiatric Diseases Ministry of Education School of Life Science ＆ Biotechnology Shanghai Jiao Tong University Shanghai 200240 China Shanghai Institute of Medical Genetics Shanghai Jiao Tong University Shanghai 200240 China School of Medicine Fudan University Shanghai 200030 China

Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location ofDby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group （35.9% vs. 95%, P = 0.001） and the as-Dby female pronucleus injection group （35.9% vs. 93.8%, P = 0.001）. The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group （17.4% vs. 57.9%, P = 0.002） and the as-Dby female pronucleus injection group （17.4% vs. 54.1%, P = 0.002）. Thus, we conclude that Dby mRNA is selectively retained in capaci- tated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.

关键词： Dby early zygotic development function mRNA spermatozoa

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山药肉类复合灌肠的配方优化研究

食品研究与开发 2017年第2期38卷 133-137页

作者：宋志强张小燕胡宏志郑州科技学院河南郑州450064

通过在灌肠配方中加入山药,原料经护色,腌制,斩拌,蒸煮等工序制得复合灌肠,增强了灌肠的营养保健功效,从而达到营养互补的目的。试验选择猪肉肥瘦质量比、山药添加量、改性玉米淀粉添加量以及山药添加方式为主要因素,研究不同因素的改... 详细信息

通过在灌肠配方中加入山药,原料经护色,腌制,斩拌,蒸煮等工序制得复合灌肠,增强了灌肠的营养保健功效,从而达到营养互补的目的。试验选择猪肉肥瘦质量比、山药添加量、改性玉米淀粉添加量以及山药添加方式为主要因素,研究不同因素的改变对山药复合灌肠感官品质的影响。试验结果表明:猪肉肥瘦质量比为1∶5,山药添加量为12%,淀粉添加量为18%,山药的添加状态为熟(糊状)时复合灌肠的弹性、组织状态、口感等最好。本研究为山药的深加工开辟了新的途径。

关键词：山药猪肉复合灌肠配比

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色素炭黑表面聚丙烯酸接枝改性制备染料

四川大学学报（自然科学版） 2003年第1期40卷 122-126页

作者：李志强赵焱陈敏四川大学皮革化学与工程教育部重点实验室成都610065

作者提出了一种基于改性炭黑的新型染料的制备方法.首先,将羟基引入到炭黑表面,然后以Ce4+为催化剂实施丙烯酸(AA)与炭黑的接枝聚合;分析与测定了接枝状况和产物的接枝率.结果表明,经上述方法改性的炭黑具有良好的水分散性和染色性.

关键词：色素炭黑聚丙烯酸接枝改性染料制备方法接枝聚合水分散性