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Potential role of novel hepatocellular carcinoma-associated gene IDD01 in promoting tumorigenesis of HepG2 cell line

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Chinese Medical Journal 2006年第20期119卷 1709-1714页

作者： CHEN Xiang-yu LI Jian-sheng ma Jun DUAN Fang-ling ZHONG peng Department of Digestive Disease Institute of Clinical Medical Research of Henan Province First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China Institute of Digestive Disease Zhengzhou University Zhengzhou 450003 China Faculty of Pharmacy Engineering College of Life Sciences JilinUniversity Changchun 130012 China

Background We have used suppression subtractive hybridization to construct a subtracted cDNA library of hepatocellular carcinoma （HCC） and isolated a panel of differential expression sequence tag （ESTs）. By using bioinformatics and rapid amplification of cDNA ends （RACE）, we found a novel HCC-associated gene IDD01. To further investigate its function, a recombinant eukaryotic vector pEGFP/ORF was constructed and transfected into the HepG2 cell line.Methods The open reading frame （ORF） of IDD01 was amplified by RT-PCR, digested with Bamh Ⅰ and Hind Ⅲ, and subcloned into the pEGFP-C1 vector. The ligation reaction was conducted with T4 DNA ligase, and the recombinant vector was named pEGFP/ORF. Untransfer control （control group）, pEGFP-C1 （HepG2/C1 group） and pEGFP/ORF （HepG2/ORF group） transfer groups were designed. Gene transfer was conducted with lipofectamine. To obtain stable transfection in HepG2 cells, selection was initiated with 500 μg/ml G418. Cellular IDD01 mRNA levels were assayed by semi-quantitative RT-PCR. The MTT colorimetric method and flow cytometry were used to determine the cell proliferation. The tumorigenic potential of transformed cells was determined from their ability to grow as anchorage-independent colonies on soft agar. Transient transfections were performed to observe subcellular location of GFP-IDD01 fusion protein.Results A 778 bp specific band of ORF was obtained by RT-PCR, and the positive clone of recombinant plasmid pEGFP/ORF （5.5 Kb） was identified by restriction endonuclease cleavage and sequence. The brightness ratio of IDD01 mRNA was not obvious between control and pEGFP/C1 groups, whereas the ratio of pEGFP/ORF was higher than that in the other two groups. After culture for 24-72 hours, the A490 values in pEGFP/ORF were higher than those in the other two groups （P〈0.01）. On histograms of flow cytometry, the S phase ratio of HepG2/ORF cells was significantly higher than that of the control and HepG2/C1 groups. The HepG2/ORF ce

关键词： hepatocellular carcinoma gene expression plasmids IDD01 gene

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畜禽饲料磷源和粪尿磷施用量对磷淋失的影响

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中国生态农业学报（中英文） 2024年第7期32卷 1218-1226页

作者：刘玲柏兆海马林中国科学院遗传与发育生物学研究所农业资源研究中心/河北省土壤生态学重点实验室/中国科学院农业水资源重点实验室石家庄050022

近年来集约化养殖业发展和畜禽粪尿的不合理还田导致大量畜禽粪尿磷流失到环境。针对该问题,我国完善了饲料磷推荐使用标准,提出了加强畜禽废弃物资源化等措施,这些措施显著降低了畜禽粪尿磷排泄量和磷环境排放。然而,将优化饲料磷与畜... 详细信息

近年来集约化养殖业发展和畜禽粪尿的不合理还田导致大量畜禽粪尿磷流失到环境。针对该问题,我国完善了饲料磷推荐使用标准,提出了加强畜禽废弃物资源化等措施,这些措施显著降低了畜禽粪尿磷排泄量和磷环境排放。然而,将优化饲料磷与畜禽粪尿施用协同管理能否降低畜禽粪尿磷淋溶风险及损失尚不明确。基于此,本文以饲喂高水溶性饲料磷源(磷酸一二钙, MDCP)和常规饲料磷源(磷酸氢钙, DCP)的畜禽粪尿为供试肥料,设计淋溶模拟试验,分析“饲料-粪尿-农田”磷素协同管理对不同形态磷淋溶量的影响,以期为我国畜禽粪尿还田的深入推进提供理论支撑。结果表明,与DCP相比, MDCP可降低仔猪、肉鸡、肉鸭和蛋鸡畜禽粪尿淋溶液中溶解性无机磷和溶解性有机磷浓度,其降低范围分别为18.2%~31.0%和19.4%~55.9%。MDCP处理降低了畜禽粪尿活性磷以溶解性无机磷和溶解性有机磷形式的损失。施用来源于DCP饲料磷源的畜禽粪尿时,仔猪、肉鸡、肉鸭和蛋鸡粪尿输入的活性磷分别有19.7%、6.9%、16.7%和6.3%以溶解性无机磷形态淋溶损失;然而,施用来源于MDCP的畜禽粪尿,上述4种畜禽粪尿输入的活性磷分别有18.3%、6.4%、12.8%和4.7%以溶解性无机磷形态淋溶损失。因此,未来为了促进畜禽粪尿科学还田,降低畜禽粪尿磷淋溶风险,建议采取“饲料-粪尿-农田”磷素协同管理措施。

关键词：饲料磷酸盐畜禽粪尿磷形态磷施用淋溶磷管理

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Smart Interfacing between Co-Fe Layered Double Hydroxide and Graphitic Carbon Nitride for High-efficiency Electrocatalytic Nitrogen Reduction

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Energy & Environmental materials 2023年第2期6卷 62-69页

作者： Xiaohui Wu Lu Tang Yang Si Chunlan ma peng Zhang Jianyong Yu Yitao Liu Bin Ding Key Laboratory of High Performance Fibers&Products(Ministry of Education) College of TextilesDonghua UniversityShanghai 201620China Innovation Center for Textile Science and Technology Donghua UniversityShanghai 200051China Jiangsu Key Laboratory of Micro and Nano Heat Fluid Flow Technology and Energy Application School of Physical Science and TechnologySuzhou University of Science and TechnologySuzhou 215009China State Center for International Cooperation on Designer Low-carbon&Environmental materials(CDLCEM) School of Materials Science and EngineeringZhengzhou UniversityZhengzhou 450001China

Bimetallic compounds such as hydrotalcite-type layered double hydroxides(LDHs)are promising electrocatalysts owing to their unique electronic ***,their abilities toward nitrogen adsorption and reduction are undermined since the surface-mantled,electronegative-OH groups hinder the charge transfer between transition metal atoms and nitrogen ***,a smart interfacing strategy is proposed to construct a coupled heterointerface between LDH and 2D g-C_(3)N_(4),which is proven by density functional theory(DFT)investigations to be favorable for nitrogen adsorption and ammonia desorption compared with neat LDH *** interfaced LDH and g-C_(3)N_(4) is further hybridized with a self-standing TiO_(2) nanofibrous membrane(NM)to maximize the interfacial effect owing to its high porosity and large surface *** from the synergistic superiorities of the three components,the LDH@C_(3)N_(4)@TiO_(2) NM delivers superior ammonia yield(2.07×10^(−9) mol s^(−1) cm^(−2))and Faradaic efficiency(25.3%),making it a high-efficiency,noble-metal-free catalyst system toward electrocatalytic nitrogen reduction.

关键词： density functional theory electrocatalytic nitrogen reduction graphitic carbon nitride interface engineering layered double hydroxide

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市域产教联合体中的多重制度逻辑冲突与协调

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职教论坛 2024年第7期40卷 5-13页

作者：马君梅文娟陕西师范大学教育学部陕西西安710062

市域产教联合体是破解我国产教融合“最后一公里”问题的关键举措。通过制度逻辑理论视角对影响市域产教联合体建设的多重制度逻辑进行分析得出,市域产教联合体的构建和运行兼具政府逻辑、市场逻辑、教育逻辑、社会逻辑和园区逻辑。不... 详细信息

市域产教联合体是破解我国产教融合“最后一公里”问题的关键举措。通过制度逻辑理论视角对影响市域产教联合体建设的多重制度逻辑进行分析得出,市域产教联合体的构建和运行兼具政府逻辑、市场逻辑、教育逻辑、社会逻辑和园区逻辑。不同的制度逻辑在市域产教联合体发展过程中呈现一定张力和矛盾冲突,主要表现为多元主体价值取向差异化、角色定位模糊化、政策制定与执行二维离散、校企合作貌合神离。因此,寻找相悖逻辑间的平衡点并进行协调是提升市域产教联合体效能的可能路径:首先应构建公共价值,回应多元主体利益诉求;其次要纵向建横向连,提高联合体治理效能;再次要创新政策执行模式,落实政策细节;最后需紧盯着力点,集聚资源优化校企合作。

关键词：市域产教联合体制度逻辑多重制度逻辑冲突协调路径

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Synergistic lubrication of few-layer Ti_(3)C_(2)T_(x)/MoS_(2) heterojunction as a lubricant additive

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Friction 2022年第12期10卷 2018-2032页

作者： peng FENG Yanping REN Yuting LI Jifan HE Zhuang ZHAO Xiaoliang ma Xiaoqiang FAN Minhao ZHU Key Laboratory of Advanced Technologies of materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Tribology Research Institute School of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China

The few-layer Ti_(3)C_(2)T_(x)/MoS_(2) heterostructure was successfully prepared via vertically growing of MoS_(2) nanosheets on the few-layer Ti3C2Tx matrix using hydrothermal *** tribological properties as additive in mineral oil(150N)were evaluated in *** 0.3 wt% of few-layer Ti_(3)C_(2)T_(x)/MoS_(2) heterostructure addition amount can reduce the friction and wear of 150N by 39% and 85%,***,the enhancement effect of few-layer Ti_(3)C_(2)T_(x)/MoS_(2) on tribological properties of 150N is superior to that of few-layer Ti_(3)C_(2)T_(x),MoS_(2) nanosheets,and their mechanical *** on the characterization and analysis of wear debris and wear track,such excellent tribological properties of the few-layer Ti_(3)C_(2)T_(x)/MoS_(2) heterostructure derive from its structural advantage toward good dispersion,the synergistic lubrication of Ti_(3)C_(2)T_(x) and MoS_(2) nanosheets during the rubbing process,and the formation of tribo-film.

关键词： few-layer Ti_(3)C_(2)T_(x) molybdenum disulfide(MoS_(2)) heterojunction tribological performance

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Advances in selective catalytic oxidation of ammonia (NH_(3)-SCO): A review of catalyst structure-activity relationship and design principles

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Chinese Chemical Letters 2024年第1期35卷 169-180页

作者： Zhao Li Chunxue Wang Junjun Qiu Yixing ma Chi Wang Xin Sun Kai Li Ping Ning Fei Wang Faculty of Environmental Science and Engineering Kunming University of Science and TechnologyKunming 650500China National-Regional Engineering Center for Recovery of Waste Gases from Metallurgical and Chemical Industries Kunming 650500China Faculty of Chemical Engineering Kunming University of Science and TechnologyKunming 650500China

NH_(3) in ambient air directly leads to an increase in the aerosol content in the air. These substances lead to the formation of haze to various environmental problems after atmospheric circulation and diffusion. Controlling NH_(3) emissions caused by ammonia escaping from mobile and industrial sources can effectively reduce the NH_(3) content in ambient air. Among the various NH_(3) removal methods, the selective catalytic oxygen method (NH_(3)-SCO) is committed to oxidizing NH_(3) to environmentally harmless H_(2)O and N_(2);therefore, it is the most valuable and ideal ammonia removal method. In this review, the characteristics of loaded and core-shell catalysts in NH_(3)-SCO have been reviewed in the context of catalyst structure-activity relationships, and the H_(2)O resistance and SO2 resistance of the catalysts are discussed in the context of practical application conditions. Then the effects of the valence state of the active center, oxygen species on the catalyst surface, dispersion of the active center and acidic sites on the catalyst performance are discussed comprehensively. Finally, the shortcomings of the existing catalysts are summarized and the catalyst development is discussed based on the existing studies.

关键词： Ammonia Selective catalytic oxidation Active metals and supports Influence of gas composition Reaction mechanism Catalyst structure-activity relationship

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Poly(vinylidene fluoride) membrane based thin film microextraction for enrichment of benzoylurea insecticides from water samples followed by their determination with HPLC

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Chinese Chemical Letters 2014年第12期25卷 1625-1629页

作者： Chen-Huan Wang Xiao-Xing ma Chun Wang Qiu-Hua Wu zhi Wang College of Science Agricultural University of Hebei No 1 High School of Baoding

Thin-film microextraction（TFME）,a new geometry for solid-phase microextraction,has become an attractive sample-preparation *** to other microextraction approaches,the sensitivity of this technique was enhanced without sacrificing the sampling time due to the high surface area-tovolume ratio together with the increase of extraction-phase *** this paper,a new TFME method based on poly（vinylidene fluoride） membrane was developed for the extraction of benzoylurea insecticides（diflubenzuron,triflumuron,hexaflumuron and teflubenzuron） from water samples followed by their determination with high performance liquid chromatography-diode array *** the optimal conditions,good linearity was observed over the concentration range of 0.5-100.0 ng/mL with correlation coefficient greater than *** limits of detection（S/N = 3） of the method for the target analytes were 0.1 ng/*** recoveries ranged from 87.7% to 103.9% with relative standard deviations lower than 6.5%.The results indicated that the developed TFME method is simple,efficient,and cost effective.

关键词： Thin film microextraction Poly(vinylidene fluoride) membrane Benzoylurea insecticides High performance liquid chromatography

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2010—2020年中国大陆副溶血性弧菌引发食源性疾病暴发调查

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中国食品卫生杂志 2024年第1期36卷 73-78页

作者：吴鹏刘继开戴月陈莉莉黄峥梁骏华付萍马洁郭云昌李宁恩施州疾病预防控制中心湖北恩施445000 国家食品安全风险评估中心北京100022 江苏省疾病预防控制中心江苏南京210009 浙江省疾病预防控制中心浙江杭州310051 福建省疾病预防控制中心福建福州350001 广东省疾病预防控制中心广东广州511400 天津市疾病预防控制中心天津300011

目的分析2010—2020年中国大陆副溶血性弧菌引起的食源性疾病暴发事件的流行病学特征,获得其发生时间和地区的分布特点以及引发事件的场所、高危食品和危险因素。方法对我国食源性疾病暴发监测系统收集的2010—2020年副溶血性弧菌引起... 详细信息

目的分析2010—2020年中国大陆副溶血性弧菌引起的食源性疾病暴发事件的流行病学特征,获得其发生时间和地区的分布特点以及引发事件的场所、高危食品和危险因素。方法对我国食源性疾病暴发监测系统收集的2010—2020年副溶血性弧菌引起的食源性疾病暴发资料进行统计分析。结果2010—2020年共上报副溶血性弧菌引起的食源性疾病暴发事件1772起,累计发病27212人,住院5944人,死亡2人。发生高峰时间是7~9月。发达地区发病率(28.8例/百万人)高于不发达地区(8.2例/百万人),沿海地区发病率(31.7例/百万人)高于内陆地区(9.2例/百万人)。病因食品以水产食品(61.2%)、肉类食品(25.3%)为主。发生场所以宾馆饭店占比最高,占49.6%。污染环节主要是烹饪水产食品时未充分烧熟煮透,加工即食肉类食品和蔬菜类食品时生熟交叉污染。结论应重点加强餐饮服务场所中水产食品和肉类食品的加工、储存等环节监管,降低副溶血性弧菌导致食源性疾病发病的风险。

关键词：食源性疾病暴发副溶血性弧菌归因分析

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韩国中小学开展人工智能教育的举措与经验

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比较教育学报 2024年第2期 163-176页

作者：夏雪景马早明华南师范大学教育科学学院国家教材建设重点研究基地华南师范大学港澳台教材综合研究基地广州510631 华南师范大学教育科学学院国际与比较教育研究所

在中小学开展人工智能教育是国际人才竞争的新焦点。基于对人工智能教育的理解,韩国在中小学人工智能教育方面积极探索有效路径,主要包括强化政策保障,顶层设计指导课程实施;职前职中并重,培育人工智能专业师资;普及AI数字教材,为学生... 详细信息

在中小学开展人工智能教育是国际人才竞争的新焦点。基于对人工智能教育的理解,韩国在中小学人工智能教育方面积极探索有效路径,主要包括强化政策保障,顶层设计指导课程实施;职前职中并重,培育人工智能专业师资;普及AI数字教材,为学生提供定制化学习内容;先锋学校引领,分享推广课程案例。这一系列举措为我国中小学人工智能教育提供了借鉴和参考的思路。

关键词：人工智能人工智能教育韩国中小学

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卧式五轴数控机床电主轴的动态特性分析及振动抑制优化

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制造技术与机床 2024年第6期 177-182页

作者：马骋邵佳煊大连理工大学机械工程学院辽宁大连116081 通用技术集团机床工程研究院有限公司大连分公司辽宁大连116620

针对某卧式五轴数控加工中心电主轴动态特性较差、测试过程中振动量大的问题,对其建立动力学模型,利用Ansys Workbench对其进行模态分析,解得固有频率及模态振型。基于仿真结果及电主轴结构原理,诊断为电主轴后端悬伸量较大,外部连接法... 详细信息

针对某卧式五轴数控加工中心电主轴动态特性较差、测试过程中振动量大的问题,对其建立动力学模型,利用Ansys Workbench对其进行模态分析,解得固有频率及模态振型。基于仿真结果及电主轴结构原理,诊断为电主轴后端悬伸量较大,外部连接法兰与主轴后轴承盖及冷却水套间隙量较大,导致机床固有频率低,动态特性差。针对上述原因对电主轴后端悬伸部增加辅助支撑优化设计,进行模态仿真分析,通过优化前后固有频率及临界转速数据对比验证其可行性。将优化设计应用于实际,并对电主轴进行振动测试,试验表明:电主轴振动量大幅减小,优化设计合理。研究结果为卧式五轴数控机床电主轴系统动态特性的改善,提供了优化思路和设计方法。

关键词：电主轴动态特性仿真分析振动抑制

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