检索结果-南通市图书馆

Towards Better Understanding of KSHV Life Cycle: from Transcription and Posttranscriptional Regulations to Pathogenesis

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第2期34卷 135-161页

作者： Lijun Yan Vladimir Majerciak zhi-ming zheng Ke Lan State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan 430072 China National Cancer Institute National Institutes of Health Frederick MD 21702 USA

Kaposi’s sarcoma-associated herpesvirus(KSHV), also known as human herpesvirus-8(HHV-8), is etiologically linked to the development of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. These malignancies often occur in immunosuppressed individuals, making KSHV infection-associated diseases an increasing global health concern with persistence of the AIDS epidemic. KSHV exhibits biphasic life cycles between latent and lytic infection and extensive transcriptional and posttranscriptional regulation of gene expression. As a member of the herpesvirus family, KSHV has evolved many strategies to evade the host immune response, which help the virus establish a successful lifelong infection. In this review, we summarize the current research status on the biology of latent and lytic viral infection, the regulation of viral life cycles and the related pathogenesis.

关键词： Kaposi's sarcoma-associated herpesvirus (KSHV) Human herpesvirus 8 (HHV-8)- Transcription Posttranscriptonal regulation ORF57

Viral Regulation of RNA Granules in Infected Cells

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第2期34卷 175-191页

作者： Qiang Zhang Nishi R. Sharma zhi-ming zheng mingzhou Chen State Key Laboratory of Virology and Modern Virology Research Center College of Life Sciences Wuhan UniversityWuhan 430072 China Tumor Virus RNA Biology Section RNA Biology Laboratory National Cancer Institute National Institutes of Health Frederick MD 21702 USA

RNA granules are cytoplasmic, microscopically visible, non-membrane ribo-nucleoprotein structures and are important posttranscriptional regulators in gene expression by controlling RNA translation and stability. TIA/G3BP/PABP-specific stress granules(SG) and GW182/DCP-specific RNA processing bodies(PB) are two major distinguishable RNA granules in somatic cells and contain various ribosomal subunits, translation factors, scaffold proteins, RNA-binding proteins, RNA decay enzymes and helicases to exclude m RNAs from the cellular active translational pool. Although SG formation is inducible due to cellular stress, PB exist physiologically in every cell. Both RNA granules are important components of the host antiviral defense. Virus infection imposes stress on host cells and thus induces SG formation. However, both RNA and DNA viruses must confront the hostile environment of host innate immunity and apply various strategies to block the formation of SG and PB for their effective infection and multiplication. This review summarizes the current research development in the field and the mechanisms of how individual viruses suppress the formation of host SG and PB for virus production.

关键词： Stress granules (SG) P-bodies (PB) RNA virus - DNA virus

HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第2期34卷 211-221页

作者： Ayslan Castro Brant Vladimir Majerciak Miguel Angelo Martins Moreira zhi-ming zheng Tumor Virus RNA Biology Section RNA Biology Laboratory Center for Cancer Research National Cancer Institute NIH Frederick MD 21702 USA Genetics Post-Graduation Program Rio de Janeiro Federal University Rio de Janeiro Brazil Genetics Program Nacional Cancer Institute INCA Rio de Janeiro 20231-050 Brazil

Human papillomavirus 18(HPV18) E6 and E7 oncogenes are transcribed as a single bicistronic E6 E7 pre-mRNA. The E6 ORF region in the bicistronic E6 E7 pre-mRNA contains an intron. Splicing of this intron disrupts the E6 ORF integrity and produces a spliced E6*I RNA for efficient E7 translation. Here we report that the E6 intron has two overlapped branch point sequences(BPS) upstream of its 30 splice site, with an identical heptamer AACUAAC, for E6*I splicing. One heptamer has a branch site adenosine(underlined) at nt 384 and the other at nt 388. E6*I splicing efficiency correlates to the expression level of E6 and E7 proteins and depends on the selection of which branch site. In general, E6*I splicing prefers the 30 ss-proximal branch site at nt 388 over the distal branch site at nt 384. Inactivation of the nt 388 branch site was found to activate a cryptic acceptor site at nt 636 for aberrant RNA splicing. Together, these data suggest that HPV18 modulates its production ratio of E6 and E7 proteins by alternative selection of the two mapped branch sites for the E6*I splicing, which could be beneficial in its productive or oncogenic infection according to the host cell environment.

关键词： Human papillomavirus 18 (HPV18) HPV splicing Branch point E6,E7,E6 intron HPV oncogenes

Boosting lithium storage performance of Si nanoparticles via thin carbon and nitrogen/phosphorus co-doped two-dimensional carbon sheet dual encapsulation

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2021年第6期40卷 1347-1356页

作者： Cheng-zhi Ke Fang Liu zhi-ming zheng He-He Zhang Meng-Ting Cai Miao Li Qi-Zhang Yan Hui-Xin Chen Qiao-Bao Zhang Department of Materials Science and Engineering College of MaterialsXiamen UniversityXiamen 361005China Department of Nanoengineering University of California San DiegoLa JollaCA 92093USA Xiamen Institute of Rare Earth Materials Haixi InstitutesChinese Academy of SciencesXiamen 361021China

Silicon(Si)is a promising anode candidate for next-generation lithium-ion batteries(LIBs),but it suffers from poor electronic conductivity and dramatic volume variation during cycling,which poses a critical challenge for stable battery *** mitigate these issues simultaneously,we propose a"double carbon synergistic encapsulation"strategy,namely thin carbon shell and nitrogen/phosphorus co-doped two-dimensional(2D)carbon sheet dual encapsulate Si nanoparticles(denoted as 2D NPC/C@Si).This double carbon structure can serve as a conductive medium and buffer matrix to accommodate the volume expansion of Si nanoparticles and enable fast electron/ion transport,which promotes the formation of a stable solid electrolyte interphase film during *** structural advantages,the resulting 2 D NPC/C@Si electrode demonstrates a high reversible capacity of592 mAh·g^(-1) at 0.2 A·g^(-1) with 90.5%excellent capacity retention after 100 cycles,outstanding rate capability(148 mAh·g^(-1) at 8 A·g^(-1)),and superior long-term cycling stability(326 mAh·g^(-1) at 1 A·g^(-1) for 500 cycles,86%capacity retention).Our findings elucidate the development of high-performance Si@C composite anodes for advanced LTBs.

关键词： Silicon@carbon composites Anode Nitrogen/phosphorus co-doped carbon Lithium-ion battery

Current Status of Human Papillomavirus-Related Head and Neck Cancer: From Viral Genome to Patient Care

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2021年第6期36卷 1284-1302页

作者： Haoru Dong Xinhua Shu Qiang Xu Chen Zhu Andreas M.Kaufmann zhi-ming zheng Andreas E.Albers Xu Qian Department of Clinical Laboratory Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou310022China Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou310022China Department of Cancer Prevention Cancer Hospital University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou310022China Clinic for Gynecology Berlin Institute of HealthCharité-Universitätsmedizin BerlinFreie Universität BerlinHumboldt-Universität Zu BerlinBerlin12203Germany Tumor Virus RNA Biology Section HIV Dynamics and Replication ProgramNational Cancer InstituteNational Institutes of HealthFrederickMD21702USA Department of Otolaryngology Head and Neck SurgeryBerlin Institute of HealthCharité-Universitätsmedizin BerlinFreie Universität BerlinHumboldt-Universität Zu BerlinBerlin13353Germany

Human papillomavirus(HPV)infection identified as a definitive human carcinogen is increasingly being recognized for its role in carcinogenesis of human *** to 38%–80%of head and neck squamous cell carcinoma(HNSCC)in oropharyngeal location(OPSCC)and nearly all cervical cancers contain the HPV genome which is implicated in causing cancer through its oncoproteins E6 and *** by the biologically distinct HPV-related OPSCC and a more favorable prognosis compared to HPV-negative tumors,clinical trials on de-escalation treatment strategies for these patients have been *** is therefore raised the questions for the patient stratification if treatment de-escalation is ***,understanding the crosstalk of HPV-mediated malignancy and immunity with clinical insights from the proportional response rate to immune checkpoint blockade treatments in patients with HNSCC is of importance to substantially improve the treatment *** review discusses the biology of HPV-related HNSCC as well as successful clinically findings with promising candidates in the pipeline for future *** the advent of various sequencing technologies,further biomolecules associated with HPV-related HNSCC progression are currently being identified to be used as potential biomarkers or targets for clinical decisions throughout the continuum of cancer care.

关键词： Human papillomavirus(HPV)integration Molecular diagnostics Oropharyngeal cancer De-Escalation treatment Immunotherapy

RGFP966 inactivation of the YAP pathway attenuates cardiac dysfunction induced by prolonged hypothermic preservation

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第9期21卷 703-715页

作者： Xiao-he zheng Lin-lin WANG ming-zhi zheng Jin-jie ZHONG Ying-ying CHEN Yue-liang SHEN Department of Basic Medicine Sciences Zhejiang University School of MedicineHangzhou 310058China Department of Orthopaedics Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Pharmacology Hangzhou Medical CollegeHangzhou 310053China Department of Obstetrics the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China

Oxidative stress and apoptosis are the key factors that limit the hypothermic preservation time of donor hearts to within 4–6 *** aim of this study was to investigate whether the histone deacetylase 3(HDAC3)inhibitor RmGodFyPn9 a6 m6 i cc opualrda mpreotteercst daugrianing srt ecpaerrdfiuasci oinn juwreyr ei nedvuacleuda *** nexgperde shsyipoont haenrdm pich *** lsh eoaf rtms awmemrea lihayn-??STE20-like kinase-1(Mst1)and Yes-associated protein(YAP)were determined by western *** apoptosis was measured by the terminal deoxynucleotidyl-transferase(TdT)-mediated dUTP nick-end labeling(TUNEL)*** of RGFP966 in Celsior solution significantly inhibited cardiac dysfunction induced by hypothermic ***966 inhibited the hypothermic preservation-induced increase of the phosphorylated(p)-Mst1/Mst1 and p-YAP/YAP ratios,prevented a reduction in total YAP protein expression,and increased the nuclear YAP protein ***(VP),a small molecular inhibitor of YAP–transcriptional enhanced associate domain(TEAD)interaction,partially abolished the protective effect of RGFP966 on cardiac function,and reduced lactate dehydrogenase activity and malondialdehyde ***966 increased superoxide dismutase,catalase,and glutathione peroxidase gene and protein expression,which was abolished by ***966 inhibited hypothermic preservation-induced overexpression of B-cell lymphoma protein 2(Bcl-2)-associated X(Bax)and cleaved caspase-3,increased Bcl-2 mRNA and protein expression,and reduced cardiomyocyte *** antioxidant and anti-apoptotic effects of RGFP966 were cancelled by *** results suggest that supplementation of Celsior solution with RGFP966 attenuated prolonged hypothermic preservation-induced cardiac *** mechanism may involve inhibition of oxidative stress and apoptosis via inactivation of the YAP pathway.

关键词： Hypothermic preservation RGFP966 Yes-associated protein(YAP) Oxidative stress Apoptosis

HPV18 DNA replication inactivates the early promoter P_(55) activity and prevents viral E6 expression

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第5期31卷 437-440页

作者： Xiaohong Wang Haibin Liu Hsu-Kun Wang Craig Meyers Louise Chow zhi-ming zheng Tumor Virus RNA Biology Section RNA Biology LaboratoryNational Cancer Institute National Institutes of Health Department of Biochemistry and Molecular Genetics University of Alabama at Birmingham Department of Microbiology and Immunology Penn State University College of Medicine

Dear Editor,Human papillomaviruses(HPV)are a large group(>200genotypes)of small double-stranded DNA viruses(https://***/).Although infections by most HPV types are asymptomatic,persistent infections in cervical and an... 详细信息

关键词： HPV18 DNA replication inactivates the early promoter P DNA activity and prevents viral E6 expression

Red cell distribution width/platelet ratio predicts decompensation of metabolic dysfunction-associated steatotic liver disease-related compensated advanced chronic liver disease

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2025年第3期31卷 1-10页

作者： ming-Hua zheng Amedeo Lonardo Department of Hepatology MAFLD Research CenterThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina Department of Internal Medicine Azienda Ospedaliero-Universitaria of Modena(2023)Modena 41126Italy

Prognostication of compensated advanced chronic liver disease(cACLD)is of paramount importance for the physician-and-patient communication and for rational clinical *** paper published by Dallio et al reports on red cell distribution width(RDW)/platelet ratio(RPR)as a non-invasive biomarker in predicting decompensation of metabolic dysfunction-associated steatotic liver disease(MASLD)-related *** from other biomarkers and algorithms,RPR is inexpensive and widely available,based on parameters which are included in a complete blood *** is computed on the grounds of two different items,one of which,RDW,mirrors the host’s response to a variety of disease stimuli and is *** second parameter involved in RPR,platelet count,is more specific and has been used in the hepatological clinic to discriminate cirrhotic from non-cirrhotic chronic liver disease for *** disease is the primary cause of mortality among MASLD subjects,followed by extra-hepatic cancers and liver-related ***,MASLD biomarkers should be validated not only in terms of liver-related events but also in the prediction of major adverse cardiovascular events and cardiovascular mortality and extra-hepatic *** sized multi-ethnic confirmatory investigation is required to define the role and significance of RPR in the stratification of MASLD-cACLD.

关键词： Cirrhosis Liver fibrosis Natural course Prognostication Stratification

基于物联网的数字唐徕信息化管理系统设计与实现

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

节水灌溉 2017年第1期 71-75页

作者：杨志顾正明宁夏回族自治区唐徕渠管理处银川750001 广东凯通软件开发有限公司广州510665

针对长期以来传统水利工程点多、面广、线长,通讯不便,管理手段落后、粗放,工程效益差,灌溉效率低,"人治"痕迹深,安全管理不合理、不到位,事故频发的问题,充分利用智能感知技术、3S技术、云计算云存储技术、物联网技术以及WebGIS等先进... 详细信息

针对长期以来传统水利工程点多、面广、线长,通讯不便,管理手段落后、粗放,工程效益差,灌溉效率低,"人治"痕迹深,安全管理不合理、不到位,事故频发的问题,充分利用智能感知技术、3S技术、云计算云存储技术、物联网技术以及WebGIS等先进信息技术,实现由传统水利向数字水利过渡,最终彻底向智慧水利转型,建立数字唐徕信息化管理系统。系统包括数字地图决策指挥平台、综合数据分析查询平台、智能灌渠专家辅助系统、掌上唐徕移动信息终端、数字唐徕渠业务管理支撑平台、自动化采集测控平台的"数字唐徕渠"的六个"一"的功能架构。是一套真正满足灌区管理需求的呈现数字化、控制智能化、决策科学化、管理协同化的灌区生产指挥管理平台,为全渠管理部门提供了科学的决策依据。

关键词：智慧水利数字唐徕信息化管理物联网 WebGIS