检索结果-南通市图书馆

Frontiers of Medicine 2010年第3期4卷 342-345页

作者： Sheng-Xuan LIU zhi-hua huang Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

The aim of this study was to investigate the value of technetium etifenin injection(^(99m)Tc-EHIDA)hepatobiliary scintigraphy in combination with determination of bilirubin from duodenal drainage in differential diagnosis between infantile hepatitis syndrome and biliary atresia.^(99m)Tc-EHIDA hepatobiliary scintigraphy in combination with duodenal fluid examination was used for evaluation in 84 infants with persistent infantile jaundice.For diagnosing biliary atresia,the sensitivity and specificity of scintigraphy were 100% and 74.5%,respectively;the sensitivity and specificity of scintigraphy in combination with duodenal fluid examination were 100% and 100%,respectively.In conclusion,hepatobiliary scintigraphy,which is a noninvasive,safe,valuable examination method,in combination with examination of duodenal fluid,is of value for the differential diagnosis between infantile hepatitis syndrome and biliary atresia.

关键词： jaundice radionuclide hepatobiliary scintigra-phy duodenal drainage

Clinical Features and Genetic Analysis of Pediatric Patients with Alagille Syndrome Presenting Initially with Liver Function Abnormalities

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2018年第2期38卷 304-309页

作者： Yan LIU Hong WANG Chen DONG Jie-xiong FENG zhi-hua huang Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Genetic Diagnostic Centre Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Alagille syndrome （AGS） is a multisystem disorder and caused by mutations in JAG1 or NOTCH2 gene. The diagnosis of AGS is hampered by its highly variable clinical manifestations. We performed a retrospective analysis on 16 children diagnosed as having AGS in recent five years in our hospital. Cholestasis was seen in 15 patients （93.8%）, heart disease in 12 （75%）, characteristic facies in 7 （43.8%）, and butterfly vertebrae in 7 （43.8%）. Ophthalmology examination was not performed on all the patients. Further, serum biochemical parameters were compared between AGS and 16 biliary atresia （BA） patients who were confirmed by surgery. Elevated liver enzymes were seen in all the patients. Serum total cholesterol （TC）（P=0.0007）, alanine aminotransferase （ALT）（P=0.0056）, aspartate aminotransferase （AST）（P=0.0114）, gamma-glutamyl transferase （GGT）（P=0.035） and total bile acid （TBA） levels （P=0.042） were significantly elevated in AGS patients compared to those in BA cases. However, there were no significant differences in serum total bilirubin （TB）, conjugated bilirubin （CB） and albumin （ALB） between the two groups. We identified 14 different JAG1 gene variations and 1 NOTCH2 gene mutation in 16 Chinese AGS patients. Our study suggested clinical features of AGS are highly variable and not all patients meet the classical diagnostic criteria. It was suggested that hypercholesterolaemia and significantly elevated GGT, TBA and ALT may be helpful to diagnose AGS. Genetic testing is integral in the diagnosis of AGS.

关键词： Alagille syndrome cholestasis biochemical characteristics gene infant

3′-Daidzein sulfonate sodium improves mitochondrial functions after cerebral ischemia/reperfusion injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第2期12卷 235-241页

作者： Wa Yuan Qin Chen Jing Zeng Hai Xiao zhi-hua huang Xiao Li Qiong Lei Gannan Medical University Ganzhou Jiangxi Province China

3′-Daidzein sulfonate sodium is a new synthetic water-soluble compound derived from daidzein（an active ingredient of the kudzu vine root）. It has been shown to have a protective effect on cerebral ischemia/reperfusion injury in rats. We plan to study the mechanism of its protective effect. 3′-Daidzein sulfonate sodium was injected in rats after cerebral ischemia/reperfusion injury. Results showed that 3′-daidzein sulfonate sodium significantly reduced mitochondrial swelling, significantly elevated the mitochondrial membrane potential, increased mitochondrial superoxide dismutase and glutathione peroxidase activities, and decreased mitochondrial malondialdehyde levels. 3′-Daidzein sulfonate sodium improved the structural integrity of the blood-brain barrier and reduced blood-brain barrier permeability. These findings confirmed that 3′-daidzein sulfonate sodium has a protective effect on mitochondrial functions after cerebral ischemia/reperfusion injury, improves brain energy metabolism, and provides protection against blood-brain barrier damage.

关键词： nerve regeneration 3′-daidzein sulfonate sodium cerebral ischemia/reperfusion injury infarct volume anti-oxidation mitochondria mitochondrial membrane swelling mitochondrial membrane potential superoxide dismutase malondialdehyde glutathione peroxidase blood-brain barrier neural regeneration

维普期刊数据库

3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2018年第9期13卷 1561-1567页

作者： Xiao Li Rui-Zhen Liu Qi Zeng zhi-hua huang Jian-Dong Zhang Zong-Liang Liu Jing Zeng Hai Xiao Gannan Medical University Ganzhou Jiangxi Province China Department of Ultrasound First Affiliated Hospital of Gannan Medical University Ganzhou liangxi Province China Department of Pathology First Affiliated Hospital of Gannan Medical University Ganzhou Jiangxi Province China

3′-Daidzein sulfonate sodium（DSS） is a new synthetic water-soluble compound derived from daidzein,a soya isoflavone that plays regulatory roles in neurobiology.In this study,we hypothesized that the regulatory role of DSS in neurobiology exhibits therapeutic effects on hippocampal damage and memory impairment.To validate this hypothesis,we established rat models of chronic cerebral hypoperfusion（CCH） by the permanent occlusion of the common carotid arteries using the two-vessel occlusion method.Three weeks after modeling,rat models were intragastrically administered 0.1,0.2,and 0.4 mg/kg DSS,once a day,for 5 successive weeks.The Morris water maze test was performed to investigate CCH-induced learning and memory deficits.TUNEL assay was used to analyze apoptosis in the hippocampal CA1,CA3 regions and dentate gyrus.Hematoxylin-eosin staining was performed to observe the morphology of neurons in the hippocampal CA1,CA3 regions and dentate gyrus.Western blot analysis was performed to investigate the phosphorylation of PKA,ERK1/2 and CREB in the hippocampal PKA/ERK1/2/CREB signaling pathway.Results showed that DSS treatment greatly improved the learning and memory deficits of rats with CCH,reduced apoptosis of neurons in the hippocampal CA1,CA3 regions and dentate gyrus,and increased the phosphorylation of PKA,ERK1/2,and CREB in the hippocampus.These findings suggest that DSS protects against CCH-induced memory impairment and hippocampal damage possibly through activating the PKA/ERK1/2/CREB signaling pathway.

关键词： nerve regeneration learning and memory deficits chronic cerebral hypoperfusion 3＇-daidzein sulfonate sodium PKAIERK1/2/CREB signaling pathway neuroprotection hippocampus neural regeneration

维普期刊数据库

Safety, pharmacokinetics, and biomarkers of F-652, a recombinant human interleukin-22 dimer, in healthy subjects

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2019年第5期16卷 473-482页

作者： Kai-Yang Tang Jason Lickliter zhi-hua huang Zong-Shu Xian Han-Yang Chen Cheng huang Chong Xiao Yu-Peng Wang Ying Tan Lin-Feng Xu Yu-Liang huang Xiao-Qiang Yan Generon(Shanghai)Corporation Ltd. Zhangjiang Hi-Tech ParkShanghaiChina Nucleus Network MelbourneVICAustralia

F-652 is a recombinant fusion protein consisting of two human interleukin-22(IL-22)molecules linked to an immunoglobulin constant region(IgG 2-Fc).IL-22 plays critical roles in promoting tissue repair and suppressing bacterial infection.The safety,pharmacokinetics(PK),tolerability,and biomarkers of F-652 were evaluated following a single dose in healthy male volunteers in a randomized,double-blind,placebo-controlled study.Following single-dose subcutaneous(SC)injection of F-652 at 2.0µg/kg into healthy subjects,six out of six subjects experienced delayed injection site reactions,which presented as erythematous and/or discoid eczematous lesions 10 to 17 days post-dosing.F-652 was then administered to the healthy subjects via an intravenous(IV)infusion at 2.0,10,30,and 45µg/kg.No severe adverse event(SAE)was observed during the study.Among the IV-dosed cohorts,eye and skin treatment emergent adverse events(TEAEs)were observed in the 30 and 45µg/kg cohorts.F-652 IV dosing resulted in linear increases in C max and AUC(0–t),and the T 1/2 ranged from 39.4 to 206h in the cohorts.An IV injection of F-652 induced dose-dependent increases in serum marker serum amyloid A,C-reactive protein,and FIB,and decreased serum triglycerides.The serum levels of 36 common pro-inflammatory cytokines/chemokines were not altered by the treatment of F-652 at 45μg/kg.In conclusion,IV administration of F-652 to healthy male volunteers is safe and well-tolerated and demonstrates favorable PK and pharmacodynamic properties.These results warrant further clinical development of F-652 to treat inflammatory diseases.

关键词： Interleukin-22 F-652 Pharmacokinetics Pharmacodynamics Safety

Alterations of biliary biochemical constituents and cytokines in infantile hepatitis syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第43期12卷 7038-7041页

作者： Yan Ding Lei Zhao Hong Mei zhi-hua huang Shu-Ling Zhang Department of Gastroenterology andHepatology Wuhan Children's Hospital Wuhan 430016 HubeiProvince China Department of Hepatology and Infectious Diseases Union Hospital Tongji Medical College ofHuazhong University of Science and Technology Wuhan 430022Hubei Province China Department of Pediatrics TongjiHospital Tongji Medical College of Huazhong University ofScience and Technology Wuhan 430030 Hubei Province China

AIM: To investigate the biliary biochemical constituents and cytokines in infantile hepatitis syndrome (IHS). METHODS: From 42 IHS subjects and 21 controls, serum and biliary biochemical constituents, including total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (γ-GT), total bile acid (TBA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) both in bile and serum, were assayed. The subjects with IHS were divided into a cholestasis group (n = 21) and a hepatitis group (n = 21). RESULTS: In the cholestasis group, serum TBIL, DBIL, ALT, γ-GT, TBA, IL-6 and TNF-α levels were higher than those in the control (P < 0.01); and also the biliary TBIL, DBIL, γ-GT and TBA levels were lower than those in the control, whereas biliary IL-6 and TNF-α levels were higher than those in the control (P < 0.01). In the cholestasis group, serum IL-6 and TNF-α levels were lower than those in bile (P < 0.01). In the hepatitis group, serum DBIL, ALT, γ-GT, TBA, IL-6 and TNF-α levels were higher than those in the control (P < 0.01 or 140.57 ± 70.32 vs 79.06 ± 35.25, P < 0.05), while biliary TBIL, DBIL, γ-GT and TBA levels were lower than those in the control (P < 0.01), and biliary IL-6 and TNF-α levels were higher than those in the control (P < 0.01). In the hepatitis group, serum IL-6 and TNF-α levels were also lower than those in bile (P < 0.01). Serum TBIL, DBIL, γ-GT, IL-6 and TNF-α levels in the cholestasis group were higher than those in the hepatitis group, while biliary IL-6 and TNF-α levels in the cholestasis group were higher than those in the hepatitisgroup. Biliary IL-6 and TNF-α were found to be more significantly increased than serum IL-6 and TNF-α in IHS (P < 0.01). The biliary IL-6 and TNF-α levels were positively correlated with serum DBIL, TBA and γ-GT levels in IHS subjects. CONCLUSION: Biliary biochemical constituents alter in coincidence with pathological changes in hepatocellular injury. Cholestasis is more serious in IHS patients of cholestasis subty

关键词：胆汁生物化学细胞活素类肝炎

Fecal microbiota transplantation induces remission of infantile allergic colitis through gut microbiota re-establishment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第48期23卷 8570-8581页

作者： Sheng-Xuan Liu Yin-Hu Li Wen-Kui Dai Xue-Song Li Chuang-Zhao Qiu Meng-Ling Ruan Biao Zou Chen Dong Yan-Hong Liu Jia-Yi He zhi-hua huang Sai-Nan Shu Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Microbial Research We Health Gene InstituteShenzhen 518000Guangdong ProvinceChina Department of Computer Science College of Science and EngineeringCity University of Hong KongHong KongChina

AIM To investigate the impact of fecal microbiota transplantation(FMT) treatment on allergic colitis(AC) and gut microbiota(GM).METHODS We selected a total of 19 AC infants,who suffered from severe diarrhea/hematochezia,did not relieve completely after routine therapy or cannot adhere to the therapy,and were free from organ congenital malformations and other contraindications for FMT. Qualified donor-derived stools were collected and injected to the AC infants via a rectal tube. Clinical outcomes and follow-up observations were noted. Stools were collected from ten AC infants before and after FMT,and GM composition was assessed for infants and donors using 16 S r DNA sequencing analysis.RESULTS After FMT treatment,AC symptoms in 17 infants were relieved within 2 d,and no relapse was observed in the next 15 mo. Clinical improvement was also detected in the other two AC infants who were lost to followup. During follow-up,one AC infant suffered from mild eczema and recovered shortly after hormone therapy. Based on the 16 S r DNA analysis in ten AC infants,most of them(n = 6) had greater GM diversity after FMT. As a result,Proteobacteria decreased(n = 6) and Firmicutes increased(n = 10) in post-FMT AC infants. Moreover,Firmicutes accounted for the greatest proportion of GM in the patients. At the genus level,Bacteroides(n = 6),Escherichia(n = 8),and Lactobacillus(n = 4) were enriched in some AC infants after FMT treatment,but the relative abundances of Clostridium(n = 5),Veillonella(n = 7),Streptococcus(n = 6),and Klebsiella(n = 8) decreased dramatically.CONCLUSION FMT is a safe and effective method for treating pediatric patients with AC and restoring GM balance.

关键词： Pediatric Infantile allergic colitis Fecal microbiota transplantation Gut microbiota Immune reaction

Chinese clinical practice guidelines for acute infectious diarrhea in children

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2018年第5期14卷 429-436页

作者： Jie Chen Chao-Min Wan Si-Tang G ong Feng F ang Mei Sun Yuan Qian Ying huang Bao-Xi Wang Chun-Di X u Li-Yan Ye Mei Dong Yu Jin zhi-hua huang Qin-Bing Wu Chao-Min Zhu You-Hong F ang Qi-Rong Zhu Yong-Sui Dong Department of Gastroenterology Children’s HospitalZhejiang University School of MedicineHangzhou 310051China Department of Pediatrics West China Second HospitalSichuan UniversityChengdu 610041China Department of Gas troenterology Guangzhou Women and Children’s Medical CenterGuangzhou 510623China Department of Pediatrics Tongji Hospital Affiliated to Tongji Medical CollegeHuazhong University of Science and T echnologyWuhan 430030China Department of Gas troenterology Shengjing Hospital of China Medical UniversityShenyang 110004China Laboratory of Virology Beijing Key Laboratory of Etiology ofV iral Diseases inChildrenCapital Institute ofP ediatricsBeijing 100020China Department of Gas troenterology Children’s Hospital of Fudan UniversityShanghai 201102China Department of Inf ectious Diseases Children’s Hospital of Fudan UniversityShanghai 201102China Department ofP ediatrics Tangdu HospitalThe Fourth Military Medical UniversityXi’an 710038China Department of Pediatrics Ruijin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 350025China Department of P ediatrics Fuzhou East HospitalFuzhou 350025China Department of P ediatrics Peking Union Medical College HospitalBeijing 100730China Department of Gas troenterology Children’s Hospital of N anjing Medical UniversityNanjing 210008China Department of Gas troenterology Children’s Hospital of Sooc how UniversitySuzhou 215003China Department of Inf ectious Diseases Children’s Hospital of Chongqing Medical UniversityChongqing 400014China

Background The guidelines addressed the evidence-based indications for the management of children with acute infectious diarrhea in Chinese pediatric population. Data sources The experts group of evidence development put forward clinical problems, collects evidence, forms prelimi-nary recommendations, and then uses open-ended discussions to form recommendations. The literature review was done for developing this guideline in databases including PubMed, Cochrane, EMBASE, China Biomedical Database, and Chinese Journal Full-text Database up to June 2013. Search the topic 'acute diarrhea' or 'enteritis' and 'adolescent' or 'child' or'Pediatric patient' or 'Baby' or 'Infant'. Results For the treatment of mild, moderate dehydration, hypotonic oral rehydration solutions (ORS) are strongly recom-mended. Intravenous (IV) rehydration is recommended for severe dehydration, with a mixture of alkali-containing dextrose sodium solution. Nasogastric feeding tube rehydration is used for children with severe dehydration without IV infusion conditions with ORS solution. Regular feeding should resume as soon as possible after oral rehydration or IV rehydration. The lactose-free diet can shorten the diarrhea duration. Zinc supplements are recommended in children with acute infectious diarrhea. Saccharomyces boulardii and Lactobacillus Rhamnus are recommended to be used in acute watery diarrhea. Sac-charomyces boulardii is recommended in children with antibiotic-associated diarrhea as well. Montmorillonite and Racec-adotril (acetorphan) can improve the symptoms of diarrhea or shorten the course of acute watery diarrhea. Antibiotics are recommended with dysenteric-like diarrhea, suspected cholera with severe dehydration, immunodeficiency, and premature delivery children with chronic underlying disease;otherwise, antibiotics are not recommended. Conclusion The principles of the most controversial treatments with of acute infectious disease are reaching to a consensus in China.

关键词： Children Diarrhea Dehydration Infection Rehydration Treatment Probiotics

Learnware:small models do big

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2024年第1期67卷 39-50页

作者： zhi-hua ZHOU zhi-Hao TAN National Key Laboratory for Novel Software Technology Nanjing University

There are complaints about current machine learning techniques such as the requirement of a huge amount of training data and proficient training skills,the difficulty of continual learning,the risk of catastrophic forgetting,and the leaking of data privacy/proprietary.Most research efforts have been focusing on one of those concerned issues separately,paying less attention to the fact that most issues are entangled in practice.The prevailing big model paradigm,which has achieved impressive results in natural language processing and computer vision applications,has not yet addressed those issues,whereas becoming a serious source of carbon emissions.This article offers an overview of the learnware paradigm,which attempts to enable users not to need to build machine learning models from scratch,with the hope of reusing small models to do things even beyond their original purposes,where the key ingredient is the specification which enables a trained model to be adequately identified to reuse according to the requirement of future users who know nothing about the model in advance.

关键词： artificial intelligence machine learning learnware

Molecular mechanism for the unusual enhancement of the second-step chemiluminescence production from the carcinogenic tetrabromohydroquinone and H2O_(2)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第7期141卷 330-342页

作者： Zi-Han Wang Chun-hua huang zhi-Sheng Liu Li Mao Ben-Zhan Zhu State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Environment and Resources University of Chinese Academy of SciencesBeijing 100049China

We have found recently that two-step intrinsic hydroxyl radical(·OH)-dependent chemiluminescence(CL)could be produced by carcinogenic tetrahaloquinone and H_(2)O_(2).However,the first-step CL was too fast to clearly detect the stepwise generation of·OH and CL,and to distinguish the exact dividing point between the first-step and second-step CL.Here we found that,extremely clear two-step intrinsic CL could be produced by the relative slow reaction of tetrabromohydroquinone(TBHQ)with H_(2)O_(2),which was directly dependent on the two-step·OH generation.Interestingly,the second-step,but not the first-step CL production of TBHQ/H_(2)O_(2)(CRET donor)was markedly enhanced by fluorescein(a typical xanthene dye,CRET acceptor)through a unique chemiluminescence resonance energy transfer(CRET)process.The novel CRET system of TBHQ/H_(2)O_(2)/fluorescein was successfully applied for the sensitive detection of TBHQ with the detection limit as low as 2.5μmol/L.These findings will help to develop more sensitive and highly efficient CL or CRET systems and specific CL sensor to detect the carcinogenic haloquinones,which may have broad environmental applications.

关键词： Two-step chemiluminescence Bromohydroquinone Hydrogen peroxide Hydroxyl radical CRET

维普期刊数据库