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Response of Polygonum viviparum Species and Community Level to Long-term Livestock Grazing in Alpine Shrub Meadow in Qinghai-Tibet Plateau

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Journal of Integrative Plant Biology 2008年第6期50卷 659-672页

作者： zhi-hong zhu Jeremy Lundholm Yingnian Li Xiaoan Wang College of Life Sciences Shaanxi Normal University Xi'an 710062 China Biology DepartmenUEnvironmental Studies Program Saint Mary's University Halifax Nova Scotia Canada B3H3C3 Northwest Institute of Plateau Biology the Chinese Academy of Sciences Xining 810008 China

Grazing by domestic herbivores is generally recognized as a major ecological factor and an important evolutionary force in grasslands, Grazing has both extensive and profound effects on individual plants and communities, We investigated the response patterns of Polygonum viviparum species and the species diversity of an alpine shrub meadow in response to long-term livestock grazing by a field manipulative experiment controlling livestock numbers on the Qinghai-Tibet Plateau in China, Here, we hypothesize that within a range of grazing pressure, grazing can alter relative allocation to different plant parts without changing total biomass for some plant species if there is life history trade-offs between plant traits, The same type of communities exposed to different grazing pressures may only alter relative species＇ abundances or species composition and not vary species diversity because plant species differ in resistant capability to herbivory, The results show that plant height and biomass of different organs differed among grazing treatments but total biomass remained constant, Biomass allocation and absolute investments to both reproduction and growth decreased and to belowground storage increased with increased grazing pressure, indicating the increasing in storage function was attained at a cost of reducing reproduction of bulbils and represented an optimal allocation and an adaptive response of the species to long- term aboveground damage, Moreover, our results showed multiform response types for either species groups or single species along the gradient of grazing intensity, Heavy grazing caused a 13,2% increase in species richness, There was difference in species composition of about 18%-20% among grazing treatment, Shannon-Wiener （H′） diversity index and species evenness （E） index did not differ among grazing treatments, These results support our hypothesis,

关键词： alpine shrub meadow biomass allocation grazing response relative species' abundances species diversity

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Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection

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Military Medical Research 2021年第4期8卷 523-534页

作者： Ming-Wang Zhang zhi-hong zhu zhi-Kuan Xia Xin Yang Wan-Ting Luo Jun-hong Ao Rong-Ya Yang Department of Dermatology Southwest HospitalArmy Medical UniversityChongqing 400038China. Department of Dermatology the Seventh Medical Center of PLA General Hospital5 NanmencangDongcheng DistrictBeijing 100700China. Department of Ophthalmology Hainan Hospital of Chinese PLA General HospitalSanya 572000HainanChina. The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510280GuangdongChina.

Background:Invasive Trichosporon asahii(***)infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of *** infection remains *** RNAs(circRNAs)are a type of endogenous noncoding RNA that participate in various disease ***,the mechanism of circRNAs in *** infection remains completely ***:RNA sequencing(RNA-seq)was performed to analyze the expression profiles of circRNAs,microRNAs(miRNAs),and mRNAs in THP-1 cells infected with *** or uninfected *** of the RNA-seq results were verified by *** Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were used to analyze the differentially expressed mRNAs.A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression ***:A total of 46 circRNAs,412 mRNAs and 47 miRNAs were differentially expressed at 12 h after *** *** and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response,the Toll-like receptor signaling pathway,and the TNF signaling pathway.A competing endogenous RNA(ceRNA)network was constructed with 5 differentially expressed circRNAs,5 differentially expressed miRNAs and 42 differentially expressed *** them,hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging ***:These data revealed a comprehensive circRNA-associated ceRNA network during *** infection,thus providing new insights into the pathogenesis of the ***-host interactions.

关键词： Trichosporon asahii Circular RNA Competing endogenous RNA RNA sequencing

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Effect of CaF_(2) and CaO/Al_(2)O_(3) on viscosity and structure of TiO_(2)-bearing slag for electroslag remelting

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Journal of Iron and Steel Research International 2021年第12期28卷 1541-1550页

作者： Jian-tao Ju Kang-shuai Yang zhi-hong zhu Yue Gu Li-zhong Chang School of Metallurgical Engineering Xi’an University of Architecture and TechnologyXi’an710055ShaanxiChina Shaanxi Engineering Research Center of Metallurgical Xi’an710055ShaanxiChina School of Metallurgy Engineering Anhui University of TechnologyMa’anshan243002AnhuiChina

The relationship between the viscosity and structure of CaF_(2)-CaO-Al_(2)O_(3)-MgO-TiO_(2)slag with different CaF_(2)contents and CaO/Al_(2)O_(3)ratios was studied using the rotating cylinder method,Fourier transform infrared spectroscopy,and Raman *** activity coefficients of CaF_(2)and the CaO/Al_(2)O_(3)ratio were determined to understand the correlation between viscosity and structure of the *** results suggest that the slag viscosity reduces gradually with an increase in CaF_(2)content from 14.1 to 28.1 wt.%or CaO/Al_(2)O_(3)ratio from 0.9 to 1.5,and correspondingly apparent activation energy for viscous flow *** addition of CaF_(2)does not change the structure of the molten slag;however,the relaxation effect of the anionic species and the hindrance effect of the cationic species are promoted by substituting part of the non-bridging oxygens(NBO)with F−ions from CaF_(2),which is attributed to the formation of NBO-Ca^(2+)-F−and NBO-Ca^(2+)-NBO,***,as the CaO/Al_(2)O_(3)ratio increases,some of the Q4 units in the aluminate structure are depolymerized into Q2 units,so that the relative strength of the Al-O-Al linkage decreases,and the relative fraction of Ti2O64−chains increases,whereas that of O-Ti-O chains decreases slightly,resulting in depolymerization on the slag ***,the effect of the CaO/Al_(2)O_(3)ratio on the structure was greater than that of CaF_(2)because of the greater depolymerization *** variation in the activity can indirectly explain the relationship between the viscosity and structure of the aluminate structural units based on thermodynamic analysis.

关键词： Viscosity TiO_(2)-bearing slag Fourier transform infrared spectroscopy Raman spectrum Thermodynamic

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Proteomic profiling of early degenerative retina of RCS rats

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 878-889页

作者： zhi-hong zhu Yan Fu Chuan-Huang Weng Cong-Jian Zhao Zheng-Qin Yin Southwest Hospital/Southwest Eye Hospital Third Military Medical University Chongqing 400038 China Key Lab of Visual Damage and Regeneration ＆ Restoration of Chongqing Chongqing 400038 China

AIM： To identify the underlying cellular and molecular changes in retinitis pigmentosa （RP）. METHODS： Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole - orbitrap MS/MS, to identify the significant differentially expressed proteins （DEPs）. Bioinformatics analyses, including Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway annotation and upstream regulatory analysis, were then performed on these retina proteins. Finally, a Western blotting experiment was carried out to verify the difference in the abundance of transcript factor E2F1. RESULTS： In this study, we identified a total of 2375 protein groups from the retinal protein samples of RCS rats and non-dystrophic congenic controls. Four hundred thirty-four significantly DEPs were selected by Student＇s t-test. Based on the results of the bioinformatics analysis, we identified mitochondrial dysfunction and transcription factor E2F1 as the key initiation factors in early retinal degenerative process. CONCLUSION： We showed that the mitochondrial dysfunction and the transcription factor E2F1 substantially contribute to the disease etiology of RP. The results provide a new potential therapeutic approach for this retinal degenerative disease.

关键词： retinal degeneration proteomics mitochondrion E2F1 MaxQuant RCS rat

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Dynamic Properties of Two-Dimensional Polydisperse Granular Gases

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Communications in Theoretical Physics 2007年第2X期48卷 343-347页

作者： LI Rui ZHANG Duan-Ming HUANG Ming-Tao SU Xiang-Ying CHEN zhi-Yuan zhu hong-Ying ZHANG Lin Department of Physics Huazhong University of Science and Technology Wuhan 430074 China

We propose a two-dimensional model of polydisperse granular mixtures with a power-law size distribution in the presence of stochastic driving. A fractal dimension D is introduced as a measurement of the inhomogeneity of the size distribution of particles. We define the global and partial granular temperatures of the multi-component mixture. By direct simulation Monte Carlo, we investigate how the inhomogeneity of the size distribution influences the dynamic properties of the mixture, focusing on the granular temperature, dissipated energy, velocity distribution, spatial clusterization, and collision time. We get the following results： a single granular temperature does not characterize a multi-component mixture and each species attains its own ＂granular temperature＂; The velocity deviation from Gaussian distribution becomes more and more pronounced and the partial density of the assembly is more inhomogeneous with the increasing value of the fractal dimension D; The global granular temperature decreases and average dissipated energy per particle increases as the value olD augments.

关键词： fractal fractal dimension polydisperse mixture

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Elevated nitrogen and co-evolution history with competitors shape the invasion process of Galinsoga quadriradiata

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Journal of Plant Ecology 2024年第4期17卷 9-22页

作者： Wen-Gang Zhang Xing-Jiang Song Laís Petri Gang Liu Xiao-Yan Chen Rui-Ling Liu Fang-Fang Huang Jia-Bin Zou zhi-hong zhu College of Life Sciences Shaanxi Normal UniversityXi’an 710119China School for Environment and Sustainability University of MichiganAnn ArborMI 48109USA Research Center for UAV Remote Sensing Shaanxi Normal UniversityXi’an 710119China Changqing Teaching&Research Base of Ecology Shaanxi Normal UniversityXi’an 710119China Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangdong Academy of ForestryGuangzhou 510520China

Invasive plants usually experience population differentiation as they expand from their initial invasive range to the ***,invasive plants usually encounter competitors which shared different co-evolutionary histories with *** factors may lead to varying responses of invasive plant populations to elevated nitrogen deposition during ***,this issue has received limited attention in prior *** address these challenges,we conducted a greenhouse experiment to investigate how population differentiation of Galinsoga quadriradiata interacts with the presence of various competitors in response to increased nitrogen *** types(new or old that shared short or long co-evolutionary history with the invader,respectively)were set to compete with the invasive central and edge populations under different nitrogen addition *** from the central population of ***,originating from the initial invasion range,showed greater total mass,reproduction and interspecific competitiveness compared with the edge *** addition improved growth and reproductive performance in both populations,and the central population had a stronger response compared with the edge *** performance of *** was inhibited more effectively by old competitors than new *** results indicate that population differentiation occurs in terms of growth and competitiveness during the range expansion of ***,with the central population exhibiting superior ***-evolutionary history with competitors is considered unfavorable for invasive plants in this *** results highlight the combined effects of population differentiation in invasive species and their co-evolution history with competitors in the context of global change factors.

关键词： Galinsoga quadriradiata central population edge population interspecific competition population differentiation elevated nitrogen

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Novel mutations in the BEST1 gene cause distinct retinopathies in two Chinese families

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International Journal of Ophthalmology(English edition) 2022年第2期15卷 205-212页

作者： zhi-hong zhu Xin Jin Yi-Xin Zhang Rui Wang Tong Wu Wei Liu Ze-Hua Chen Hai-Nan Xie Lan-Lan Chen Zi-Hao Liu Hou-Bin Huang Department of Ophthalmology Hainan Hospital of Chinese PLA General HospitalSanya 572013Hainan ProvinceChina Department of Ophthalmology Chinese PLA General HospitalBeijing 100853China Department of Ophthalmology the PLA Rocket Force Characteristic Medical CenterBeijing 100088China Department of Ophthalmology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina

AIM:To describe the clinical heterogeneity of patients with novel mutations in ***:All the members in the two Chinese families underwent detailed clinical evaluations including best-corrected visual acuity,slit-lamp examination,applanation tonometry,and dilated fundus *** autofluorescence,fundus fluorescein angiography,spectral-domain optical coherence tomography,electrooculography,and electroretinogram were also *** DNA was extracted from venous blood for all the *** targeted next-generation sequencing of inherited retinal disease-associated genes was conducted to identify the causative ***:A novel BEST1 missense mutation c.41T>C(***14Ser) was identified in Family *** was co-segregated with the phenotype of best vitelliform macular dystrophy(BVMD) and bioinformatics analysis confirmed it was *** novel BEST1 frameshift mutation c.345_(3)46insGGCAAGGACG(***119Glyfs*116) and a novel USH2A missense mutation c.12560G>A,***4187 His were identified in family 2 with retinitis pigmentosa(RP),which might interact and lead to the phenotype of ***:Two novel mutations in the BEST1 gene in two unrelated families with distinct phenotypes and BEST1 mutation accompanied with USH2A mutation would result in RP,which could be enormously helpful in understanding the pathogenesis of the inherited retinal disease caused by a BEST1 mutation.

关键词： BEST1 gene best vitelliform macular dystrophy retinitis pigmentosa gene mutation

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入侵植物粗毛牛膝菊根际土壤和根系真菌群落会沿海拔扩散路径发生变化

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Journal of Plant Ecology 2023年第1期16卷 105-122页

作者： Rui-Ling Liu Wen-Gang Zhang Benjamin R.Lee Gang Liu Xing-Jiang Song Xiao-Yan Chen Jia-Bin Zou Fang-Fang Huang zhi-hong zhu College of Life Sciences Shaanxi Normal UniversityXi’an 710119China Carnegie Museum of Natural History PittsburghPA 15213USA Department of Biological Sciences University of PittsburghPittsburghPA 15213USA Holden Forest and Gardens KirtlandOH 44094USA National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China Shaanxi Normal UniversityXi’an 710119China Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangdong Academy of ForestryGuangzhou 510520China

与入侵植物相关的真菌群落可能会随着海拔发生变化,这可能会影响入侵植物的表现和入侵力。我们最近的研究表明,入侵植物粗毛牛膝菊(Galinsoga quadriradiata)的根系AMF侵染率随着海拔的升高而下降。然而,尚不清楚这是否与真菌群落沿海... 详细信息

与入侵植物相关的真菌群落可能会随着海拔发生变化,这可能会影响入侵植物的表现和入侵力。我们最近的研究表明,入侵植物粗毛牛膝菊(Galinsoga quadriradiata)的根系AMF侵染率随着海拔的升高而下降。然而,尚不清楚这是否与真菌群落沿海拔的变化相关。为此,我们采用高通量测序技术、功能分组和线性分析等方法,研究了秦巴山区的粗毛牛膝菊根际土壤和根系真菌群落组成与结构沿海拔的变化趋势。研究结果表明,粗毛牛膝菊根际土壤和根系真菌群落物种多样性和种类组成随海拔升高发生了显著变化。在根际土壤中真菌群落香农-威纳多样性指数随着海拔升高呈上升趋势,而在根系中则呈下降趋势。同时,在根际土壤中共生真菌的相对丰度随海拔高度没有发生显著变化,而在根系中呈下降趋势;在根际土壤中病原真菌的相对丰度随着海拔呈下降趋势,而在根系中呈上升趋势。这些研究结果暗示,该种在向研究区域高海拔地区入侵时,土壤共生真菌的供给并不会缺乏,但其与这些真菌之间建立并维持共生关系的能力降低了;在向高海拔扩张过程中,该种倾向于在根系中积累更多的病原真菌,而减少对共生真菌的依赖。这些研究结果说明,入侵植物与真菌群落之间的相互作用会沿着海拔梯度发生显著变化,植物地下部分与真菌的相互作用可能是我们理解外来种如何在高海拔环境中入侵成功的关键。

关键词：入侵植物范围扩张真菌多样性真菌丰度共生菌病原菌

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变长分组Banyan网中排队的神经网络调度

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上海交通大学学报 2001年第6期35卷 866-869页

作者：李生红薛质诸鸿文潘理上海交通大学电子工程系上海200030

提出了适用于变长分组情形下进行调度的系统结构 ;给出了分组调度的具体原则 ;基于所给出的调度原则 ,研究了利用 Hopfield神经网络实现优化调度的方法和在硬件电路上的可实现性 .

关键词： Banyan网神经网络排队多级互联网络分组调度通信网分组网交换结构

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阳性神经元面积百分比和染色灰度相对强度值的结合在大鼠脑组织免疫组化图像定量分析中的应用

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中国应用生理学杂志 2017年第3期33卷 282-286页

作者：曾志刚邱红朱梅菊朱洪竹萧建华井冈山大学体育学院井冈山大学附属医院儿科江西吉安343009

目的:通过下丘脑弓状核乙酰胆碱表达的检测、图像处理、图像分析,应用阳性神经元面积百分比(APPN)和染色灰度相对强度值(RISGL)相结合的方法,探讨免疫组化(IHC)图像定量分析的特点。方法:样本来源于运动性免疫抑制过程中SD大鼠下丘脑弓... 详细信息

目的:通过下丘脑弓状核乙酰胆碱表达的检测、图像处理、图像分析,应用阳性神经元面积百分比(APPN)和染色灰度相对强度值(RISGL)相结合的方法,探讨免疫组化(IHC)图像定量分析的特点。方法:样本来源于运动性免疫抑制过程中SD大鼠下丘脑弓状核胆碱能阳性神经元乙酰胆碱表达的免疫组化切片,包括第0、2、4、6周与每周Control、Immediately after exercise、3 hours after exercise三组共计12组(n=6)。采用免疫组化技术测定其乙酰胆碱(ACh)的表达,并根据ACh阳性神经元总面积(TAPN)、阳性神经元灰度平均强度值(AISGL)、APPN、RISGL、APPN/RISGL参数进行免疫组化图像的定量分析,从而比较APPN和RISGL在定量分析中与传统参数的不同及其优势。结果:APPN与TAPN的变化呈现几乎一致的变化特征,能发现相应差异的显著性意义(P<0.05),但APPN的敏感性和抗干扰性更强。RISGL与AISGL呈现的结果并不完全一致,而且APPN结合RISGL比单参数更能反映阳性表达情况。结论:APPN和RISGL免疫组化图像分析参数能较可靠地、准确地进行图像定量分析,APPN与RISGL的结合不仅可反映阳性表达的数量情况,还可以帮助分析其作用机理,优于传统分析参数。

关键词：免疫组化图像分析面积灰度大鼠

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