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Molecular characterization and expression of HSP70, HSF and HSBP genes in Octopus vulgaris during thermal stress

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Acta Oceanologica Sinica 2015年第8期34卷 62-72页

作者： hong Jingni MAO Yong niu Sufang SUN Tiantian SU Yongquan College of Ocean and Earth Sciences Xiamen University

Temperature is an important environmental factor that affects the growth and survival of Octopus vulgaris, the common octopus. To understand the protective mechanism that O. vulgaris exhibits under heat stress, we used rapid amplification of cDNA ends （RACE） to obtain full-length sequences of three heat stress response related genes：（1） the heat shock protein 70 （OvHSPT0）, （2） the heat shock transcription factor （OvHSF）, and （3） the heat shock factor-binding protein （OvHSBP） of O. vulgaris. The OvHSP70, OvHSF, and OvHSBP proteins contained 2 222 bp, 2 264 bp, 841 bp that encoded for 635, 458 and 90 amino acids, respectively. The results of multiple sequence alignment showed that the amino acid sequences of OvHSP70 were highly conserved with respect to other species. Similarly, the DNA binding domain, the trimerization domain of OvHSF, and the coiled coil region of OvHSBP also had highly conserved regions. The real-time polymerase chain reaction （PCR） results indicated that OvHSP70 was temperature-dependent and time-dependent, showing a positive response to heat stress. On exposure to 26℃ and to 30℃, the mRNA expression levels of OvHSF and OvHSBP were higher than those in the control group at 24℃. The mRNA expression of OvHSBP significantly increased with heat treatment at 26℃, while the mRNA expression of OvHSF decreased. The experimental results indicated that the expression of OvHSP70, OvHSF and OvHSBP were all sensitive to heat stress, which suggests that these three genes may play an important role for O. uulgaris in responding to environmental stress. Thus, this study sets a theoretical foundation for further in-depth studies on the molecular protective mechanisms of the heat response in O. vulgaris.

关键词： Octopus vulgaris high temperature stress HSP70 HSF HSBP

BHONEM:Binary High-Order Network Embedding Methods for Net worked-Guarantee Loans

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Journal of Computer Science & Technology 2019年第3期34卷 657-669页

作者： Da-Wei Cheng Yi Tu Zhen-Wei Ma zhi-Bin niu Li-Qing Zhang Department of Computer Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Mathematical Science Shanghai Jiao Tong University.Shanghai 200240China School of Computer Software Tianjin UniversityTianjin 300354China

Networked-guarantee loans may cause systemic risk related concern for the government and banks in *** prediction of the default of enterprise loans is a typical machine learning based classification problem, and the networked guarantee makes this problem very difficult to solve. As we know, a complex network is usually stored and represented by an adjacency matrix. It is a high-dimensional and sparse matrix, whereas machine-learning methods usually need lowdimensional dense feature representations. Therefore, in this paper, we propose a binary higher-order network embedding method to learn the low-dimensional representations of a guarantee network. We first set vertices of this heterogeneous economic network by binary roles (guarantor and guarantee), and then define high-order adjacent measures based on their roles and economic domain knowledge. Afterwards, we design a penalty parameter in the objective function to balance the importance of network structure and adjacency. We optimize it by negative sampling based gradient descent algorithms,which solve the limitation of stochastic gradient descent on weighted edges without compromising efficiency. Finally, we test our proposed method on three real-world network datasets. The result shows that this method outperforms other start-of-the-art algorithms for both classification accuracy and robustness, especially in a guarantee network.

关键词： networked-guarantee loan high-order network embedding representative learning gradient descent

Analysis of solute preferential transport in a dark coniferous forest ecosystem of Gongga Mountain,Sichuan Province,southwestern China

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Forestry Studies in China 2010年第1期12卷 14-20页

作者： niu Jian-zhi YU Xin-xiao ZHANG zhi-qiang Key Laboratory of Soil and Water Conservation ＆ Desertification Combating of the Ministry of Education School of Soil and WaterConservation Beijing Forestry University Beijing 100083 P. R. China

We selected a dark coniferous forest ecosystem of Gongga Mountain in the upper reaches of the Yangtze River as our research area to study the preferential flow and solute preferential transport by adding the tracers KNO3 and KBr to the self-made soil column equipment in different ways to examine density and volume changes of inflows and outflows of a mass input （impulse input） and a stable, well-distributed input （step input））. The results showed that this dark coniferous forest ecosystem of Gongga Mountain is a typical area of preferential flow and solute preferential transport, a process that can be classified into five parts. A great amount of solute was transported at high speed as the result of preferential flow in the soil and caused the density of the solute in both deep water and in groundwater to rise rapidly, which definitely increased pollution in the deep soil layer.

关键词： Gongga Mountain dark coniferous forest ecosystem preferential flow preferential transport solute transport,breakthrough curve water quality

Efficient and robust CNN-LSTM prediction of flame temperature aided light field online tomography

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Science China(Technological Sciences) 2024年第1期67卷 271-284页

作者： niu zhiTian QI hong SUN AnTai REN YaTao HE MingJian GAO BaoHai School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China Key Laboratory of Aerospace Thermophysics Ministry of Industry and Information TechnologyHarbin 150001China

Light field tomography,an optical combustion diagnostic technology,has recently attracted extensive attention due to its easy implementation and ***,the conventional iterative methods are high data throughput,low efficiency and time-consuming,and the existing machine learning models use the radiation spectrum information of the flame to realize the parameter field measurement at the current *** is still an offline measurement and cannot realize the online prediction of the instantaneous structure of the actual turbulent combustion *** this work,a novel online prediction model of flame temperature instantaneous structure based on deep convolutional neural network and long short-term memory(CNN-LSTM)is *** method uses the characteristics of local perception,shared weight,and pooling of CNN to extract the threedimensional(3D)features of flame temperature and outgoing radiation ***,the LSTM is used to comprehensively utilize the ten historical time series information of high dynamic combustion flame to accurately predict 3D temperature at three future moments.A chaotic time-series dataset based on the flame radiation forward model is built to train and validate the performance of the proposed CNN-LSTM *** is proven that the CNN-LSTM prediction model can successfully learn the evolution pattern of combustion flame and make accurate predictions.

关键词： temperature prediction convolutional neural network long short-term memory light field imaging online tomography

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Synthesis and anti-HBV activity of novel 5-substituted pyridin-2(1H)-one derivatives

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Chinese Chemical Letters 2010年第3期21卷 290-292页

作者： Zhang, Yi Kai Lv, zhi Liang niu, Chun Juan Li, Ke Second Mil Med Univ Dept Med Chem Sch Pharm Shanghai 200433 Peoples R China

Four novel 5-substituted pyridine-2(1H)-one derivatives were designed and synthesized by using addition-elimination *** structures of these novelly synthesized compounds were verified by ~1H NMR,ESI-MS and single crystal X-ray ***,all four compounds(most notably compound 7a) were found to be highly efficient against hepatitis B virus (HBV) in cultured HepG2 2.2.15 cell,making them promising drug candidates for potential bioactive molecule against hepatitis B.

关键词： 5-Substituted pyridin-2(1H)-one Synthesis Crystal structure Anti-HBV activity

Bio-inspired Backstepping Adaptive Sliding Mode Control for Parallel Mechanism with Actuation Redundancy

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International Journal of Automation and computing 2014年第5期11卷 555-564页

作者： Xue-Mei niu Guo-Qin Gao Xin-Jun Liu zhi-Ming Fang School of Electrical and Information Engineering Jiangsu University Department of Mechanical Engineering Tsinghua University

This paper presents a bio-inspired backstepping adaptive sliding mode control strategy for a novel 3 degree of freedom(3-DOF) parallel mechanism with actuation redundancy. Based on the kinematic model and the dynamic model, a sliding mode controller is designed to assure the tracking performance, and an adaptive law is introduced to approximate the system uncertainty including parameters variation, external disturbances and un-modeled part. Furthermore, a bio-inspired model is introduced to solve the inherent chattering problem of sliding mode control and provide a chattering free control. The simulation and experimental results testify that the proposed bio-inspired backstepping adaptive sliding mode control can achieve better performance(the tracking accuracy,robustness, response speed, etc.) than the conventional slide mode control.

关键词： Parallel mechanism redundant actuation kinematics analysis dynamics analysis adaptive sliding mode control bioinspired model

Sliding Mode Control of Hydraulic Pressure in Electro-Hydraulic Brake System Based on the Linearization of Higher-Order Model

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Fluid Dynamics & Materials Processing 2020年第3期16卷 513-524页

作者： Qiping Chen Haoyu Sun Ning Wang zhi niu Rui Wan Key Laboratory of Conveyance and Equipment Ministry of Education East China Jiaotong UniversityNanchang330013China

The possibility to enhance the stability and robustness of electrohydraulic brake(EHB)systems is considered a subject of great importance in the automotive *** such a context,the present study focuses on an actuator with a four-way sliding valve and a hydraulic cylinder.A 4-order nonlinear mathematical model is introduced *** the linearization of the feedback law of the high order EHB model,a sliding mode control method is proposed for the hydraulic *** hydraulic pressure tracking controls are simulated and analyzed by MATLAB/Simulink soft considering separately different conditions,i.e.,a sine wave,a square wave and a square wave with superimposed sine *** results show that the proposed strategy can track the target within 0.25 s,and the mean observed error is less than 1.2 ***,with such a strategy,faster response and less overshoot are possible,which should be regarded as significant advantages.

关键词： EHB hydraulic pressure feedback linearization sliding mode control

Ultrafast pulse-dilation framing camera and its application for time-resolved X-ray diagnostic

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Nuclear Science and Techniques 2024年第7期35卷 101-114页

作者： Hou-zhi Cai Qiu-Yan Luo Kai-Xuan Lin Xuan Deng Jun-Kai Liu Kai-zhi Yang Dong Wang Jia-Jie Chen Jia-Heng Wang Jing-Hua Long Li-hong niu Yun-Fei Lei Jin-Yuan Liu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen Key Laboratory of Photonics and BiophotonicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen518060China

An ultrafast framing camera with a pulse-dilation device,a microchannel plate(MCP)imager,and an electronic imaging system were *** camera achieved a temporal resolution of 10 ps by using a pulse-dilation device and gated MCP imager,and a spatial resolution of 100μm by using an electronic imaging system comprising combined magnetic *** spatial resolution characteristics of the camera were studied both theoretically and *** results showed that the camera with combined magnetic lenses reduced the field curvature and acquired a larger working area.A working area with a diameter of 53 mm was created by applying four magnetic lenses to the ***,the camera was used to detect the X-rays produced by the laser-targeting *** diagnostic results indicated that the width of the X-ray pulse was approximately 18 ps.

关键词： Inertial confinement fusion Plasma diagnostics Framing camera Combined lenses Pulse-dilation

Dynamics and Control of a Novel 3-DOF Parallel Manipulator with Actuation Redundancy

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Machine Intelligence Research 2013年第6期10卷 552-562页

作者： Xue-Mei niu Guo-Qin Gao Xin-Jun Liu zhi-Da Bao School of Electrical and Information Engineering Jiangsu University Department of Mechanical Engineering Tsinghua University

This paper deals with the dynamics and control of a novel 3-degrees-of-freedom(DOF)parallel manipulator with actuation *** to the kinematics of the redundant manipulator,the inverse dynamic equation is formulated in the task space by using the Lagrangian formalism,and the driving force is optimized by utilizing the minimal 2-norm *** on the dynamic model,a synchronized sliding mode control scheme based on contour error is proposed to implement accurate motion tracking ***,an adaptive method is introduced to approximate the lumped uncertainty of the system and provide a chattering-free *** simulation results indicate the efectiveness of the proposed approaches and demonstrate the satisfactory tracking performance compared to the conventional controller in the presence of the parameter uncertainties and un-modelled dynamics for the motion control of manipulators.

关键词： Parallel manipulator redundant actuation dynamics modelling sliding mode control weighed integral gain reaching law