检索结果-南通市图书馆

Identification of a delayed leaf greening gene from a mutation of pummelo

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第7期64卷 1165-1173页

作者： Hui-Wen Yu zhi-hao lu Xia Wang Dan Liu Jia-Xian He Xiao-Lin Jiang Ling-Jun Ke Wen-Wu Guo Xiu-Xin Deng Qiang Xu Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics ofFujian Colleges and Universities Minnan Normal UniversityZhangzhou 363000China

Delayed greening of young leaves is an unusual phenomenon of plants in *** are mostly evergreen tree ***,a natural mutant of“Guanxi”pummelo(Citrus maxima),which shows yellow leaves at the young stage,was characterized to identify the genes underlying the trait of delayed leaf greening in plants.A segregating population with this mutant as the seed parent and a normal genotype as the pollen parent was *** DNA pools respectively from the leaves of segregating seedlings with extreme phenotypes of normal leaf greening and delayed leaf greening were collected for *** segregant analysis(BSA)and In Del marker analysis demonstrated that the delayed leaf greening trait is governed by a 0.3 Mb candidate region on chromosome *** expression analysis further identified a key candidate gene(Citrus Delayed Greening gene 1,CDG1)in the 0.3 Mb region,which showed significantly differential expression between the genotypes with delayed and normal leaf greening *** was a 67 bp In Del region difference in the CDG1 promoter and the In Del region contains a TATA-box *** laser-scanning microscopy revealed that the CDG1-GFP fusion protein signals were co-localized with the chloroplast signals in the *** of CDG1 in tobacco and Arabidopsis led to the phenotype of delayed leaf *** results suggest that the CDG1 gene is involved in controlling the delayed leaf greening phenotype with important functions in chloroplast development.

关键词： citrus pummelo delayed leaf greening chlorophyll

Multiple hypointense vessels on susceptibility-weighted imaging predict early neurological deterioration in acute ischaemic stroke patients with severe intracranial large artery stenosis or occlusion receiving intravenous thrombolysis

在线全文

学校读者我要写书评

暂无评论

Stroke & Vascular Neurology 2020年第4期5卷 361-367页

作者： Yong-Lin Liu Han-Peng Yin Dong-Hai Qiu Jian-Feng Qu Huo-Hua Zhong zhi-hao lu Fang Wang Man-Qiu Liang Yang-Kun Chen Department of Neurology Dongguan People’s HospitalDongguanGuangdongChina Department of Radiology Dongguan People’s HospitalDongguanGuangdongChina

background and purpose Early neurological deterioration(END)is a common feature in patients with acute ischaemic stroke(AIS)receiving *** study aimed to investigate whether the presence of multiple hypointense vessels(MHVs)on susceptibility-weighted imaging(SWI)could predict END in patients with the anterior circulation AIS treated with recombinant tissue plasminogen activator(r-tPA).Methods This was a retrospective study focusing on AIS patients suffering from symptomatic stenosis or occlusion of the middle cerebral artery or internal carotid artery with r-tPA *** collected clinical variables and initial haematological and neuroimaging *** were measured on SWI performed after intravenous thrombosis and were defined as the presence of a greater number of veins or veins of a larger diameter with greater signal loss on SWI than those of the contralesional *** degree of hyperintensity of MHVs was classified into four grades:none,subtle,moderate and *** was defined as an increase in the National Institutes of Health Stroke Scale score by 2 points during the first 48 hours after the onset of *** logistic regressions were conducted to investigate the predictors of *** The study included 61 patients(51 males and 10 females)with a mean age of 62.4±12.6 ***-five(57.4%)patients presented with MHVs:8(13.1%)were graded as subtle MHVs,while 23(37.7%)and 4(6.6%)were graded as moderate or extensive MHVs,*** patients(32.8%)presented with *** regression analysis showed that compared with patients without MHVs,moderate MHVs(adjusted OR 5.446,95%CI 1.360 to 21.800;p=0.017)and extensive MHVs(adjusted OR 15.240,95%CI 1.200 to 193.544;p=0.036)were significantly associated with *** MHVs might be a useful predictor of END in AIS patients with symptomatic large artery stenosis or occlusion after r-tPA treatment.

关键词： patients stenosis intravenous

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

胡杨叶总黄酮提取物的体内抗氧化活性研究

新疆农业科学 2011年第8期48卷 1438-1443页

作者：王安银郝志录张君白红进塔里木大学生命科学学院新疆阿拉尔843300 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室新疆阿拉尔843300

【目的】以胡杨叶子为研究对象,测定其总黄酮提取物的体内抗氧化活性。【方法】用超声辅助法提取胡杨叶中的总黄酮,并采用CCl_4肝损伤小鼠模型测定胡杨叶中的总黄酮提取物的体内抗氧化活性。【结果】胡杨叶中的总黄酮提取物可以显著提... 详细信息

【目的】以胡杨叶子为研究对象,测定其总黄酮提取物的体内抗氧化活性。【方法】用超声辅助法提取胡杨叶中的总黄酮,并采用CCl_4肝损伤小鼠模型测定胡杨叶中的总黄酮提取物的体内抗氧化活性。【结果】胡杨叶中的总黄酮提取物可以显著提高小鼠血清和肝组织中SOD、GSH-PX的活性,并降低它们的MDA含量。【结论】胡杨叶中的总黄酮提取物具有一定的体内抗氧化活性,为其进一步研究提供了参考依据。

关键词：胡杨叶总黄酮超声辅助法体内抗氧化活性

A mechanical reinforced and antifreezing polyacrylate hydrogel electrolyte for high-performance zinc-ion batteries

在线全文

学校读者我要写书评

暂无评论

Nano Research 2025年第1期18卷 341-350页

作者： Zili Zhang Ruolin Wang Hongfei lu Di Zhang Yu Zhao Jing Xu Bin Sun zhi-Min Dang Yang Jin School of Electrical and Information Engineering Zheng Zhou UniversityZhengzhou 450001China State Key Lab of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

The operation of aqueous zinc-ion batteries in flexible energy storage field is plagued by the uncontrollable growth of Zn-dendrite and inevitable freeze of water below 0 ℃.Therefore,it is necessary to design a hydrogel electrolyte with good mechanical property and freezing resistance to uniform the Zn-deposition and resist flexibility loss at low *** find that the mechanical property(strength and toughness)of hydrogel electrolyte has a significant impact on the suppression of dendrite growth and the uniform deposition of zinc ***,a polyacrylate hydrogel is prepared in one step by ultraviolet(UV)curing method with Zn(CF_(3)SO_(3))_(2)salt and polyvinyl alcohol(PVA)addition to increase the antifreezing ability and mechanical *** adsorption of water molecules by 2-hydroxyethyl acrylate(HEA)and PVA reduces the freezing point of the hydrogel,which is beneficial for enhancing the electrochemical stability at low *** this basis,the Zn-symmetrical battery with hydrogel electrolyte has a long lifespan of 4710 h at 0.5 mA·cm^(-2)and 0.5 mAh·cm^(-2)at room ***,the hydrogel electrolyte exhibits an outstanding stability at low temperature of^(-2)0 ℃,the lifespan of symmetrical battery reaches to 4000 h at 0.5 mA·cm^(-2)and 0.5 mAh·cm^(-2).The assembled full cell with NaV3O8·1.5H_(2)O(NVO)cathode and hydrogel electrolyte possesses a high capacity retention ratio of 77%after 10,000 cycles at^(-2)0 ℃.The flexible cell can power light-emitting diode(LED)lamp under bending,warping and cutting without liquid leakage and an electronic watch at the operating temperature of^(-2)0 ℃.

关键词： zinc ion battery hydrogel electrolyte mechanical enhancement freezing resistance

High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2611-2623页

作者： Mengshi Yang Miao Bai Yuan Zhuang Shenghua lu Qianqian Ge hao Li Yu Deng Hongbin Wu Xiaojian Xu Fei Niu Xinlong Dong Bin Zhang Baiyun Liu Beijing Tiantan Hospital Capital Medical UniversityBeijingChina Department of Neurology The First Hospital of Tsinghua UniversityBeijingChina Department of Neurotrauma and Beijing Key Laboratory of Central Nervous System Injury Beijing Neurosurgical InstituteCapital Medical UniversityBeijingChina Department of Critical Care Medicine Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Center for Nerve Injury and Repair Beijing Institute of Brain DisordersChina National Clinical Research Center for Neurological DiseasesBeijingChina

Although microglial polarization and neuroinflammation are crucial cellular responses after traumatic brain injury,the fundamental regulatory and functional mechanisms remain insufficiently *** potent anti-inflammato ry agents,the use of glucoco rticoids in traumatic brain injury is still controversial,and their regulatory effects on microglial polarization are not yet *** the present study,we sought to determine whether exacerbation of traumatic brain injury caused by high-dose dexamethasone is related to its regulatory effects on microglial polarization and its mechanisms of *** vitro cultured BV2 cells and primary microglia and a controlled cortical impact mouse model were used to investigate the effects of dexamethasone on microglial ***,dexamethasone,RU486(a glucocorticoid receptor antagonist),and ruxolitinib(a Janus kinase 1 antagonist)were ***-sequencing data obtained from a C57BL/6 mouse model of traumatic brain injury were used to identify potential targets of *** Morris water maze,quantitative reverse transcription-polymerase chain reaction,western blotting,immunofluorescence and confocal microscopy analysis,and TUNEL,Nissl,and Golgi staining were performed to investigate our ***-throughput sequencing results showed that arginase 1,a marker of M2 microglia,was significantly downregulated in the dexamethasone group compared with the traumatic brain injury group at3 days post-traumatic brain *** dexamethasone inhibited M1 and M2 microglia,with a more pronounced inhibitory effect on M2microglia in vitro and in *** receptor plays an indispensable role in microglial polarization after dexamethasone treatment following traumatic brain ***,glucocorticoid receptor activation increased the number of apoptotic cells and neuronal death,and also decreased the density of dendritic spines.A possible downstream receptor signaling mechanism is th

关键词： apoptosis BV2 microglia dexamethasone glucocorticoid receptor glucocorticoids innate immune system microglial polarization neuroinflammation primary microglia traumatic brain injury

在线全文

学校读者我要写书评

暂无评论

X期鸡胚简易体外培养方法的探索

天津农学院学报 2003年第4期10卷 36-38页

作者：郝志明卢祺天津农学院动物科学系天津300384

以原蛋壳为孵化蛋壳,利用塑料袋对鸡胚进行转壳,用保鲜膜或蛋壳皿和石蜡封口,分别对I、II两种简易方法的X期鸡胚体外培养进行了研究。实验结果表明,方法I组鸡胚蚊虫珠期的发育率为72.7%(8 11);方法I、II组鸡胚8日龄存活率分别为0(0 11)... 详细信息

以原蛋壳为孵化蛋壳,利用塑料袋对鸡胚进行转壳,用保鲜膜或蛋壳皿和石蜡封口,分别对I、II两种简易方法的X期鸡胚体外培养进行了研究。实验结果表明,方法I组鸡胚蚊虫珠期的发育率为72.7%(8 11);方法I、II组鸡胚8日龄存活率分别为0(0 11)和17.6%(3 17)。

关键词：鸡胚体外培养原蛋壳

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

在线全文

学校读者我要写书评

暂无评论

Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang zhi-Lai Zhan Guang-lu Chang Yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation

药效学结合正交试验优选参连颗粒剂的醇提工艺

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国中药杂志 2017年第4期42卷 702-707页

作者：王敏杜守颖陈笑南田志浩陆洋白洁武慧超张勤帅万洁北京中医药大学北京100102 北京中医药大学东方医院北京100078

对参连方中醇提部分工艺进行优选。在以致豚鼠心律失常时哇巴因的消耗量为药效指标,考察全方水提,全方水提醇沉,党参等水提醇沉,丹参等醇提和党参等水提、丹参等醇提4种不同提取工艺路线的基础上,采用L9(34)正交表进行正交实验,以不同... 详细信息

对参连方中醇提部分工艺进行优选。在以致豚鼠心律失常时哇巴因的消耗量为药效指标,考察全方水提,全方水提醇沉,党参等水提醇沉,丹参等醇提和党参等水提、丹参等醇提4种不同提取工艺路线的基础上,采用L9(34)正交表进行正交实验,以不同实验方案的出膏率及丹酚酸B、丹参酮Ⅱ_A、小檗碱3种指标成分的含量为评价指标,研究乙醇浓度、乙醇用量、提取时间、提取次数4个因素对醇提部分提取工艺的影响,应用综合评分法优选最佳提取工艺,并进行工艺验证试验。所得最佳提取工艺为加入60%乙醇共提取2次,分别加入10,9倍量、提取2.0 h,丹酚酸B、丹参酮Ⅱ_A、小檗碱提取量分别为42.01,0.41,40.15 mg·g^(-1)。优选的工艺稳定、可行,可为参连颗粒的工业化生产提供依据。

关键词：参连方醇提正交试验多指标加权综合评分法

Grain-boundary segregation and grain growth in nanocrystalline substitutional solid solution alloys

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2024年第1期168卷 239-249页

作者： Fawei Tang Chao Hou hao lu zhi Zhao Xiaoyan Song Faculty of Materials and Manufacturing Key Laboratory of Advanced Functional MaterialsMinistry of Education of ChinaBeijing University of TechnologyBeijing 100124China

A model for describing solute segregation at grain boundaries has been developed for substitutional solid solution alloys,which integrates multiple factors from atomic to microstructural scales.A concept of mo-lar Gibbs free energy of segregation was introduced to evaluate the segregating capability of the solute elements in a closed system,through which the influences of grain boundary structure,grain size,ma-terial composition,and external conditions were *** on the evaluation of various energy forms related to solute segregation and grain growth processes,the nature of the thermal stabilization of nanograin structures by solute segregation was disclosed.A criterion for the destabilization of nanostruc-tures,which is determined by the competition of the change rates between the molar Gibbs free energy of segregation and the total energy of grain boundaries with grain size,has been *** study provided guideline to achieve high-temperature stability of nanograin structures of solid solution alloys even for the weakly segregating nanocrystalline systems.

关键词： Substitutional solid solution Grain boundary segregation Thermal stabilization Solute concentration Grain size