检索结果-南通市图书馆

山羊流产嗜衣原体OmpA基因重组质粒构建及对小鼠免疫效果研究

在线全文

学校读者我要写书评

暂无评论

中国畜牧兽医 2016年第11期43卷 2886-2891页

作者：龙冲冲邓位喜毛以智曾贵英伍波涛熊朝丽徐茂文夏鹏文明遵义市农业委员会遵义563000 贵州大学动物科学学院贵阳550025

为探讨山羊流产嗜衣原体重组真核质粒进入临床试验的可行性,本试验用PCR方法扩增出山羊流产嗜衣原体OmpA基因,克隆至真核表达载体pcDNA3.1(+)中,经PCR和双酶切鉴定后,将重组质粒pcDNA3.1-OmpA转染至PK-15细胞,观察目的基因表达情况,并... 详细信息

为探讨山羊流产嗜衣原体重组真核质粒进入临床试验的可行性,本试验用PCR方法扩增出山羊流产嗜衣原体OmpA基因,克隆至真核表达载体pcDNA3.1(+)中,经PCR和双酶切鉴定后,将重组质粒pcDNA3.1-OmpA转染至PK-15细胞,观察目的基因表达情况,并将制备的大肠杆菌基因组单链DNA作为分子佐剂与重组质粒共同免疫小鼠,检测重组质粒在小鼠体内分布情况及血清抗体水平。结果表明,经PCR、双酶切和测序鉴定表明成功构建重组质粒pcDNA3.1-OmpA;转染PK-15细胞后,荧光抗体试验结果证实重组质粒pcDNA3.1-OmpA得到有效表达。首免后14d,重组质粒加分子佐剂组的抗体效价显著高于重组质粒组和灭活疫苗组(P<0.05);随着免疫次数增加和时间推移,免疫小鼠抗体水平均呈现上升趋势,至35d时达到最高峰,此后抗体滴度逐渐下降,但仍维持较高水平。首免后21d,在小鼠心脏、肝脏、脾脏、肾脏、肺脏、脑、空肠和腿肌中均可检测到质粒的分布,此后逐渐消失,49d在所检组织中均未检测到重组质粒的存在。表明试验成功构建了基于OmpA基因的山羊流产嗜衣原体重组真核质粒,且加入分子佐剂后可诱导小鼠产生较高水平的血清抗体。

关键词：流产嗜衣原体 OmpA基因分子佐剂免疫效果

Reaction Kinetics in Anionic Copolymerization： A Revisit on Mayo-Lewis Equation

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2016年第4期34卷 431-438页

作者： zhi-chao wu Yu Liu Wei Wei Fang-shu Chen gui-xue Qiu 熊辉明 School of Polymer Science and Engineering Qingdao University of Science and Technology Qingdao 266042 China Department of Polymer Science Shanghai Jiao Tong University Shanghai 200240 China

Reactivity ratio is a traditional parameter quantifying the reaction kinetics in copolymerization, which is important for potentially controlling microstructures of polymers and guiding the copolymerization process. Our recent experiments using tube-NMR technique enable us to in situ monitor the concentration profiles of the co-monomers during the anionic copolymerization process. This motivates us to revisit the Mayo-Lewis （ML） equation, which is the basis for derivation of reactivity ratio and has been extensively utilized in addition copolymerization. We found that although an explicit ML expression is desirable for ease of calculation and correlation with experimental data, it fails in our anionic copolymerization experiment as well as some data available in the literature. The origin is ascribed to the validity of the steady state assumption which is essential in the ML equation. This assumption can be released in anionic copolymerization and replaced by the fact that the overall concentration of the living chain ends keeps constant throughout the copolymerization. Alternative numerical method has been utilized to obtain the rate constants and consequently the reactivity ratios. Our work suggests that the ML equation should be applied with caution.

关键词： Anionic copolymerization Polyether Reactivity ratio Mayo-Lewis equation Steady state assumption.

Brain-derived neurotrophic factor plays with TRiC:focus on synaptic dysfunction in Huntington’s disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2919-2920页

作者： Yingli Gu Kijung Sung Chengbiao wu Department of Neurosciences University of California San DiegoLa JollaCAUSA

Brain-derived neurotrophic factor(BDNF)exerts pleiotropic effects on brain processes including psychiatric disorders,aging,neurodegeneration,and metabolic homeostasis.A simple PubMed search using the key word“BDNF,”to date,yields over 33,000 *** fundamental biology to potential therapeutic applications,BDNF has clearly garnered extensive and significant attention in the field of neurobiology research.

关键词： Huntington homeostasis therapeutic

Loss of stat3 function leads to spine malformation and immune disorder in zebrafish

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2017年第3期62卷 185-196页

作者： Shuting Xiong Junjie wu Jing Jing Peipei Huang zhi Li Jie Mei Jian-Fang gui College of Fisheries Key Laboratory of Freshwater Animal BreedingMinistry of AgricultureFreshwater Aquaculture Collaborative Innovation Center of Hubei ProvinceHuazhong Agricultural University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences

STAT （Signal Transducers and Activators of Transcription） gene family members have been revealed to be involved in cell growth and differentiation in vertebrates. Despite their physiological importance, their functions are poorly studied at organ and systemic levels. In this study, we performed a genome-wide analysis using data from invertebrates to vertebrates to identify STAT genes and analyze their evolutionary history. Interestingly, the STAT gene family undergoes genome duplications during the evolutionary history with STAT3 homologues firstly appearing in the basal extant vertebrate, sea lamprey, suggesting its possible roles in spine formation. To investigate the functions of stat3 in fish species, TALEN technology was performed to generate mutant zebrafish lines, star3 mutant zebrafish showed no obvious defects at early developmental stage but displayed severe lateral and vertical curvature of the spine （scoliosis）, spine fracture and the incomplete bone joints with narrower junction between vertebrae at early juvenile stage, as indicated by Alizarin red and Alcian blue staining, radiography and micro-computed tomography （MicroCT） analysis. Transcriptome analysis reveals dramatic alterations in a number of genes involved in immune and infection response, skeletal development and somatic growth, especially downregulated expression of collagen gene family, in the juvenile stat3 mutant zebrafish. Moreover, most of the collagen genes were detected to have abnormal expression pattern during the formation of spine deformities in stat3 mutants. Our data reveal that stat3 is specially expressed in vertebrates and required for normal spine development and immune function in zebrafish.

关键词： Star3 Zebrafish Spine malformation Immune disorder

Reliability of a Novel Cobb Protractor for Measuring the Cobb Angle of Radiograph in Scoliosis

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2015年第1期30卷 18-22页

作者： gui wu Hai Wang Ran Ding Xu-hong Xue zhi-hong wu gui-xing Qiu Department of Orthopaedics Peking Union Medical College HospitalChinese Academy of Medical Sciences & Peking Union Medical College

Objective To introduce a novel Cobb protractor and assess its reliability and rapidity for measuring Cobb angle in scoliosis patients. Methods The novel Cobb protractor had two endplate markers. A measurement was performed just to align the two markers to each endplate of the curve. The Cobb angle on the posteroanterior radiographs of 24 patients clinically diagnosed with adolescent idiopathic scoliosis was measured by three orthopedic surgeons with both standard Cobb method and the new technique, and the time of measurement was recorded. Intraclass correlation coefficients(ICCs) were calculated to assess the reliability of the new method. Results The time for a measurement with the new tool was approximately 10 seconds less than the time that used to finish a measurement with the standard method(P<0.05). The overall mean Cobb angle for the major curve of the 24 patients was 47.8°. The mean overall intraobserver and interobserver ICC was 0.971 and 0.971 for the Cobb method group, while the overall intraobserver ICC and the interobserver was 0.985 and 0.979 for the new tool group. Conclusions The novel Cobb protractor could perform quick measurement and measure almost all forms of radiographs. The Cobb protractor might be an ideal instrument to measure the Cobb angle.

关键词： Cobb protractor reliability measurement scoliosis Cobb angle

Neuronal regulated cell death in aging-related neurodegenerative diseases:key pathways and therapeutic potentials

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2245-2263页

作者： Run Song Shiyi Yin Jiannan wu Junqiang Yan Department of Neurology The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina Neuromolecular Biology Laboratory The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina

Regulated cell death(such as apoptosis,necroptosis,pyroptosis,autophagy,cuproptosis,ferroptosis,disulfidptosis)involves complex signaling pathways and molecular effectors,and has been proven to be an important regulatory mechanism for regulating neuronal aging and ***,excessive activation of regulated cell death may lead to the progression of aging-related *** review summarizes recent advances in the understanding of seven forms of regulated cell death in age-related ***,the newly identified ferroptosis and cuproptosis have been implicated in the risk of cognitive impairment and neurodegenerative *** forms of cell death exacerbate disease progression by promoting inflammation,oxidative stress,and pathological protein *** review also provides an overview of key signaling pathways and crosstalk mechanisms among these regulated cell death forms,with a focus on ferroptosis,cuproptosis,and *** instance,FDX1 directly induces cuproptosis by regulating copper ion valency and dihydrolipoamide S-acetyltransferase aggregation,while copper mediates glutathione peroxidase 4 degradation,enhancing ferroptosis ***,inhibiting the Xc-transport system to prevent ferroptosis can increase disulfide formation and shift the NADP^(+)/NADPH ratio,transitioning ferroptosis to *** insights help to uncover the potential connections among these novel regulated cell death forms and differentiate them from traditional regulated cell death *** conclusion,identifying key targets and their crosstalk points among various regulated cell death pathways may aid in developing specific biomarkers to reverse the aging clock and treat age-related neurodegenerative conditions.

关键词： apoptosis autophagy cuproptosis disulfidptosis ferroptosis necroptosis neurodegenerative disease neurological aging diseases PANoptosis pyroptosis

在线全文

学校读者我要写书评

暂无评论

区域最优人口规模测评模型研究

武汉理工大学学报 2004年第8期26卷 93-95页

作者：黄明知罗荣桂吴兵李文军武汉理工大学系统工程研究所武汉430070

提出了 3大类区域最优人口规模测评模型 ,它们分别是适用于农业文明时代的相对土地资源承载力模型和人口优属度模型、适用于工业文明时代的 Com moner方程反算模型和适用于知识经济时代的基于 HDI- GPC基准意义下的最优人口规模模型。... 详细信息

提出了 3大类区域最优人口规模测评模型 ,它们分别是适用于农业文明时代的相对土地资源承载力模型和人口优属度模型、适用于工业文明时代的 Com moner方程反算模型和适用于知识经济时代的基于 HDI- GPC基准意义下的最优人口规模模型。以上海市为例 ,对模型的应用做了相应的说明。

关键词：区域最优人口规模测评模型

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： Fei Lv Longjie Liu Jiazhe wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

Maximizing deviation method for multiple attribute decision making under q-rung orthopair fuzzy environment

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2020年第5期16卷 1073-1087页

作者： Jie Wang gui-wu Wei Cun Wei Jiang wu School of Business Sichuan Normal UniversityChengdu610101PR China School of Statistics Southwestern University of Finance and EconomicsChengdu611130PR China

Because of the uncertainty and subjectivity of decision makers in the complex decision-making environment,the evaluation information of alternatives given by decision makers is often fuzzy and *** a generalization of intuitionistic fuzzy set(IFSs)and Pythagoras fuzzy set(PFSs),q-rung orthopair fuzzy set(q-ROFS)is more suitable for expressing fuzzy and uncertain ***,in actual multiple attribute decision making(MADM)problems,the weights of DMs and attributes are always completely unknown or partly known,to date,the maximizing deviation method is a good tool to deal with such ***,combine the q-ROFS and conventional maximizing deviation method,we will study the maximizing deviation method under q-ROFSs and q-RIVOFSs in this ***,we briefly introduce the basic concept of q-rung orthopair fuzzy sets(q-ROFSs)and q-rung interval-valued orthopair fuzzy sets(q-RIVOFSs).Then,combine the maximizing deviation method with q-rung orthopair fuzzy information,we establish two new decision making *** this basis,the proposed models are applied to MADM problems with q-rung orthopair fuzzy *** with existing methods,the effectiveness and superiority of the new model are *** method can effectively solve the MADM problem whose decision information is represented by q-rung orthopair fuzzy numbers(q-ROFNs)and whose attributes are incomplete.

关键词： Multiple attribute decision making(MADM) q-rung orthopair fuzzy sets(q-ROFSs) q-rung interval-valued orthopair fuzzy sets (q-RIVOFSs) Maximizing deviation method Building materials