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Specific shRNA targeting of FAK influenced collagen metabolism in rat hepatic stellate cells

World Journal of Gastroenterology 2010年第32期16卷 4100-4106页

作者： zhi-Na Dun Xiao-Lan Zhang Jun-Yan An li-Bo Zheng Robert Barrett Shu-Rui Xie Department of Gastroenterology The Second Hospital of Hebei Medical University Hebei Key Laboratory of Gastroenterology Hebei Institute of Gastroenterology Shijiazhuang Hebei Province China Inflammatory Bowel Disease Center and Immunobiology Research Institute Cedars-Sinai Medical Center Los Angeles CA 90048 United States

AIM:To investigate the effects and mechanism of disruption of focal adhesion kinase(FAK) expression on collagen metabolism in rat hepatic stellate cells(HSC).METHODS:The plasmids expressing FAK short hairpin RNA(shRNA) were transfected into HSC-T6 cells,and the level of FAK expression was determined by both real-time quantitative polymerase chain reaction(QPCR) and Western blotting *** production of type collagen and type collagen in FAK-disrupted cells was analyzed by real-time *** level of collagen metabolism proteins,including matrix metalloproteinases-13(MMP-13) and tissue inhibitors of metalloproteinases-1(TIMP-1) was also determined by both real-time Q-PCR and Western blotting ***:The transfection of FAK shRNA plasmids into HSC resulted in disrupted FAK *** with the HK group,the levels of type collagen and type collagen mRNA transcripts in FAK shRNA plas-mid group were signif icantly decreased(0.69 ± 0.03 vs 1.96 ± 0.15,P = 0.000;0.59 ± 0.07 vs 1.62 ± 0.12,P = 0.020).The production of TIMP-1 in this cell type was also signif icantly reduced at both mRNA and protein levels(0.49 ± 0.02 vs 1.72 ± 0.10,P = 0.005;0.76 ± 0.08 vs 2.31 ± 0.24,P = 0.000).However,the expression of MMP-13 mRNA could be significantly up-regulated by the transfection of FAK shRNA plasmids into HSC(1.74 ± 0.20 vs 1.09 ± 0.09,P = 0.000).CONCLUSION:These data support the hypothesis that shRNA-mediated disruption of FAK expression could attenuate extracellular matrix(ECM) synthesis and promote ECM degradation,making FAK a potential target for novel anti-f ibrosis therapies.

关键词： Focal adhesion kinase Hepatic stellate cells Matrix metalloproteinases RNA interference Type collagen Tissue inhibitors of metallo-proteinases

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Secure coverage-preserving node scheduling scheme using energy prediction for wireless sensor networks

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The Journal of China Universities of Posts and Telecommunications 2010年第5期17卷 100-108页

作者： li zhi-yuan,WANG Ru-chuan. College of Computer,Nanjing University of Posts and Telecommunications,Nanjing 210003,China College of Computer Nanjing University of Posts and Telecommunications Nanjing 210003 China

With the fast development of the micro-electro-mechanical systems （MEMS）,wireless sensor networks （WSNs） have been extensively *** of the studies focus on saving energy consumption because of restricted energy supply in ***-based node scheduling scheme is commonly considered as one of the most energy-efficient ***,it is not always so efficient especially when there exist hot spot and network attacks in *** this article,a secure coverage-preserved node scheduling scheme for WSNs based on energy prediction is proposed in an uneven deployment *** scheme is comprised of an uneven clustering algorithm based on arithmetic progression,a cover set partition algorithm based on trust and a node scheduling algorithm based on energy *** results show that network lifetime of the scheme is 350 rounds longer than that of other scheduling ***,the scheme can keep a high network coverage ratio during the network lifetime and achieve the designed objective which makes energy dissipation of most nodes in WSNs balanced.

关键词： wireless sensor networks secure energy-efficient coverage node scheduling cover set energy prediction

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OptiVol fluid index predicts acute decompensation of heart failure with a high rate of unexplained events

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Journal of Geriatric Cardiology 2013年第3期10卷 253-257页

作者： Xin-Wei Yang Wei Hua li-Gang Ding Jing Wang li-Hui Zheng Chong-Qiang li zhi-Min liu Ke-Ping Chen Shu Zhang Center of Arrhythmia Diagnosis and Treatment Fuwai Cardiovascular Hospital Chinese Academy of Medical Sciences Peking Union Medical College No.167 Beilishi Road Xicheng District Beijing 100037 China

Background Intrathoracic impedance monitoring has emerged as a promising new technique for the detection of impending heart failure (HF). Although false positive episodes have been reported in case reports and clinical trials, the efficacy and false positive rate in real-world practice remain unclear. Objective The aim of this study is to investigate the utility and reliability of the OptiVol alert feature in clinical practice. Methods We continuously recruited patients who underwent implantable cardioverter-defibrillator (ICD) or cardiac re-synchronization therapy with defibrillator (CRT-D) implantation with feature of intrathoracic impedance monitoring system in our center from Sep. 2010 to Oct. 2012. Regular in-office follow-up were required of all patients and the following information was collected at each visit: medical history, device interrogation, N-terminal pro-brain natriuretic peptide (NT-proBNP) measurement and an echocardiogram. Worsening HF was defined as hospitalization or the presentation of signs or symptoms of HF. Results Forty three patients (male:76.7%, mean age:57 ± 15 years, left ventricular ejection fraction (LVEF):33%± 14%) were included in this observational study. Fifty four alert events and 14 adjudicated worsening HF were detected within 288 ±163 days follow-up. Eleven (20.4%) alert episodes were associated with acute cardiac decompensation in 9 patients with a positive predictive value of 78.6%. Forty three audible alerts showed no connection to worsening HF. The unexplained alerts rate was 79.6%and 1.27 per person-year. Thirty seven alarm alerts were detected in patients with EFlic function also exhibited considerable alert events. The OptiVol fluid index predicted worsenin

关键词： Heart failure Intrathoracic impedance measurement Left ventricular ejection fraction OptiVol fluid index

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Association between PPARG genetic polymorphisms and ischemic stroke risk in a northern Chinese Han population: a case-control study

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Neural Regeneration Research 2019年第11期14卷 1986-1993页

作者： Yan-Zhe Wang He-Yu Zhang Fang liu Lei li Shu-Min Deng zhi-Yi He Department of Neurology the First Hospital of China Medical University

Two common polymorphisms of the peroxisome proliferator-activated receptor gamma(PPARG) gene, rs1801282 and rs3856806, may be important candidate gene loci affecting the susceptibility to ischemic stroke. This case-control study sought to identify the relationship between these two single-nucleotide polymorphisms and ischemic stroke risk in a northern Chinese Han population. A total of 910 ischemic stroke participants were recruited from the First Hospital of China Medical University, Shenyang, China as a case group, of whom 895 completed the study. The 883 healthy controls were recruited from the Health Check Center of the First Hospital of China Medical University, Shenyang, China. All participants or family members provided informed consent. The study protocol was approved by the Ethics Committee of the First Hospital of China Medical University, China on February 20, 2012(approval No. 2012-38-1). The protocol was registered with the Chinese Clinical Trial Registry(registration number: ChiCTR-COC-17013559). Plasma genomic DNA was extracted from all participants and analyzed for rs1801282 and rs3856806 single nucleotide polymorphisms using a SNaPshot Multiplex sequencing assay. Odds ratios(ORs) and 95% confidence intervals(CIs) were calculated using unconditional logistic regression to estimate the association between ischemic stroke and a particular genotype. Results demonstrated that the G allele frequency of the PPARG gene rs1801282 locus was significantly higher in the case group than in the control group(P < 0.001). Individuals carrying the G allele had a 1.844 fold increased risk of ischemic stroke(OR = 1.844, 95% CI: 1.286–2.645, P < 0.001). Individuals carrying the rs3856806 T allele had a 1.366 fold increased risk of ischemic stroke(OR = 1.366, 95% CI: 1.077–1.733, P = 0.010). The distribution frequencies of the PPARG gene haplotypes rs1801282-rs3856806 in the control and case groups were determined. The frequency of distribution in the G-T haplotype case

关键词： nerve regeneration stroke cerebral ischemia ischemic stroke peroxisome proliferator-activated receptor γ single-nucleotide polymorphism haplotype analysis interaction case-control study Chinese Han population neural regeneration

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Monopole effects, core excitations, and β decay in the A = 130 hole nuclei near ^(132)Sn

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Chinese Physics C 2019年第5期43卷 35-40页

作者： Han-Kui Wang zhi-Hong li Cen-Xi Yuan zhi-Qiang Chen Ning Wang Wei Qin Yi-Qi He College of Physics and Telecommunication Engineering Zhoukou Normal University China Institute of Atomic Energy Sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen University School of Physics and State Key Laboratory of Nuclear Physics and Technology Peking University College of mathematics and statistics Zhoukou Normal University

The proton and neutron cross-shell excitations across the Z = 50 shell are investigated in the southwest quadrant of ^(132) Sn by large-scale shell-model calculations with extended pairing and multipole-multipole force. The model space allows proton(neutron) core excitations, and both the low-and high-energy states for ^(130) In are well described, as found by comparison with the experimental data. The monopole effects between the proton orbit and neutron orbit are studied as the new monopole correction that perfectly reproduces the first 1^+ level in ^(130) In. The energy interval of proton(neutron) core excitations in ^(130) In lies in the range of 4.5-6.5(2.0-4.1) MeV, and the high energy yrast states are predicted as neutron core excitations. The decays are calculated among the A=130 nuclei of ^(130) In, ^(130) Sn and ^(130) Cd.

关键词： monopole effects core-excitations β decay hole nuclei

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Real-time motion deblurring algorithm with robust noise suppression

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第5期11卷 375-380页

作者： Hua-jun FENG Yong-pan WANG zhi-hai XU Qi li Hua LEI Ju-feng ZHAO State Key Lab of Modern Optical Instrumentation Zhejiang University Hangzhou 310027 China

In an image restoration process,to obtain good results is challenging because of the unavoidable existence of noise even if the blurring information is already *** suppress the deterioration caused by noise during the image deblurring process,we propose a new deblurring method with a known ***,the noise in the measurement process is assumed to meet the Gaussian distribution to fit the natural noise ***,the first and second orders of derivatives are supposed to satisfy the independent Gaussian distribution to control the non-uniform *** results show that our method is obviously superior to the Wiener filter,regularized filter,and Richardson-Lucy(RL) ***,owing to processing in the frequency domain,it runs faster than the other algorithms,in particular about six times faster than the RL algorithm.

关键词： Motion blurring Motion kernel Gaussian distribution

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Pharmaceuticals and personal care products transference-transformation in aquifer system

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Journal of Groundwater Science and Engineering 2020年第4期8卷 358-365页

作者： YUAN Qiao-ling li zhi-ping li Lei-cheng WANG Shu-li YAO Si-yu College of Geosciences and Engineering North China University of Water Resources and Electric PowerZhengzhou450046China

Pharmaceuticals and personal care products(PPCPs)are a new kind of contaminant widely existing in the surface water and groundwater *** recent years,PPCPs have been received widely attention from many *** migration and transformation of PPCPs are mainly photolysis,biodegradation,adsorption and hydrolysis in aquifer *** influencing factors of PPCPs migration include PPCPs’own physical and chemical properties,types and contents of organic matter,pH,lithology,geotechnical structure and the thickness of vadose zone,*** present,the research of PPCPs in China is still in the primary stage,especially on the contaminant in aquifer ***,the research in this field needs to be further strengthened.

关键词： PPCPs Migration and transformation Influencing factor Contaminant

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A simple,water-soluble,Fe^(3+)-selective fluorescent probe and its application in bioimaging

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Chinese Chemical Letters 2015年第1期26卷 129-132页

作者： Xiao-Hang Yang Shu li zhi-Shu Tang Xi-Di Yu Tin Huang Yong Gao Medical Technology Department.Shaanxi College of Traditional Chinese Medicine College of Chemistry and Chemical Engineering Fujian Normal University State Key Laboratory of Fine Chemicals Dalian University of Technology

A simple, water-soluble, Fe3＋-selective fluorescent probe, derived from rhodamine B, was synthesized and characterized. The probe exhibits a fluorescence response toward Fe3＋ with acceptable sensitivity and selectivity and even facilitates visual or naked-eye detection of Fe3＋. The experiment results show that the response of the probe to Fe3＋ is pH-independent over a wide range of 4.0-10.0. In addition, fluorescence microscopic imaging experiments have proven that the probe is cell permeable and can be used for monitoring intracellular Fe3＋ in living cells.

关键词： Fluorescent probe Ferric ion Aqueous solution Fluorescent imaging

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Dose response and time course of manganeseenhanced magnetic resonance imaging for visual pathway tracing in vivo

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Neural Regeneration Research 2016年第7期11卷 1185-1190页

作者： Wei-ling Wang Hui Xu Ying li zhi-zhong Ma Xiao-dong Sun Yun-tao Hu Department of Ophthalmology Beijing Tsinghua Changgung HospitalTsinghua University Medical Center Department of Ophthalmology General Hospital of Ningxia Medical University Department of Radiology Peking University Third Hospital Peking University Eye Center Peking University Third HospitalKey Laboratory of Vision Loss and RestorationMinistry of Education Department of Ophthalmology Shanghai Jiao Tong University Affiliated First People's Hospital

Axonal tracing is useful for detecting optic nerve injury and regeneration,but many commonly used methods cannot be used to observe axoplasmic flow and synaptic transmission in ***（Mn^2＋）-enhanced magnetic resonance imaging（MEMRI） can be used for in vivo longitudinal tracing of the visual ***,we explored the dose response and time course of an intravitreal injection of Mn Cl2 for tracing the visual pathway in rabbits in vivo using *** found that 2 m M Mn Cl2 enhanced images of the optic nerve but not the lateral geniculate body or superior colliculus,whereas at all other doses tested（5–40 m M）,images of the visual pathway from the retina to the contralateral superior colliculus were significantly *** images were brightest at 24 hours,and then decreased in brightness until the end of the experiment（7 days）.No signal enhancement was observed in the visual cortex at any concentration of Mn *** results suggest that MEMRI is a viable method for temporospatial tracing of the visual pathway in *** enhancement in MEMRI depends on the dose of Mn Cl2,and the strongest signals appear 24 hours after intravitreal injection.

关键词： tracing detecting optic brightness retina viable synaptic until cortex fitting

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Interactions between cadmium and multiple precipitates in an Al-li-Cu alloy:Improving aging kinetics and precipitation hardening

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Journal of Materials Science & Technology 2020年第11期42卷 44-49页

作者： liang Wu Yugang li Xianfeng li Naiheng Ma Haowei Wang State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai200240China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai200240China

This work demonstrates significant improvements in both the aging kinetics and precipitation hardening of an Al-li-Cu alloy with the minor addition of Cd(0.06 at.%).The precipitation hardening effect of T1 precipitates in casting Al-li-Cu alloys has long been ignored since it is difficult to achieve a high number density of fine precipitates without a large number of dislocations.A detailed transmission electron microscopy investigation shows that the Cd addition has changed the distribution of T1 precipitates from the conventional uneven distribution near dislocations or grain boundaries to a more homogeneous *** of the Cd-rich nanoparticles were observed at the broad face and/or terminal of the T1 *** is highly likely that these nanoparticles act as heterogeneous nucleation sites,which consequently leads to a higher number density of T1 ***,Cd atoms were preferentially segregated withinδprecipitates,which can be attributed to the strong bonding between li and *** interactions between Cd and the T1(Al2Culi)andδ′(Al3li)precipitates in Al-li-Cu alloy are first *** present study may propose a new mechanism to effectively improve precipitation kinetics and therefore the age-hardening effect of Al-li-Cu alloys.

关键词： Aluminum alloys Three-dimensional atom probe(3daP) High-angle annular dark-field(HAADF) Precipitation Cadmium

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