检索结果-南通市图书馆

Trillium tschonoskii maxim extract attenuates abnormal Tau phosphorylation

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2018年第5期13卷 915-922页

作者： Hong-Bin Luo Nan Shang wen-zhi xie De-Jian wen Min Qu Sheng Huang Sha-Sha Fan Wei Chen Nan-Qiao Mou Xiang-Yu Liu Qin Chen Feng-Feng xie Jun-Xu Li Hubei Key Laboratory of Biological Resource Protection and Utilization Hubei University for Nationalities Medical College Hubei University for Nationalities Institute of Neurological and Psychiatric Comorbidity Hubei University for Nationalities Hubei Provincial Key Laboratory for Applied Toxicology Hubei Provincial Center for Disease Control and Prevention Department of Pharmacology and Toxicology Jacobs School of Medicine and Biomedical Sciences University at Buffalo State University of New York

Large-scale epidemiological studies have found that hyperhomocysteinemia is a powerful, independent risk factor for Alzheimer＇s disease. Trillium tschonoskii maxim is a traditional Chinese medicine that is used to promote memory. However, scientific understanding of its mechanism of action is limited. This report studied the potential neuroprotective effects of Trillium tschonoskii maxim extract against homocysteine-induced cognitive deficits. Rats were intravenously injected with homocysteine（400 μg/kg） for 14 days to induce a model of Alzheimer＇s disease. These rats were then intragastrically treated with Trillium tschonoskii maxim extract（0.125 or 0.25 g/kg） for 7 consecutive days. Open field test and Morris water maze test were conducted to measure spontaneous activity and learning and memory abilities. Western blot assay was used to detect the levels of Tau protein and other factors involved in Tau phosphorylation in the hippocampus. Immunohistochemical staining was used to examine Tau protein in the hippocampus. Golgi staining was applied to measure hippocampal dendritic spines. Our results demonstrated that homocysteine produced learning and memory deficits and increased levels of Tau phosphorylation, and diminished the activity of catalytic protein phosphatase 2A. The total number of hippocampal dendritic spines was also decreased. Trillium tschonoskii maxim extract treatment reversed the homocysteine-induced changes. The above results suggest that Trillium tschonoskii maxim extract can lessen homocysteine-induced abnormal Tau phosphorylation and improve cognitive deterioration such as that present in Alzheimer＇s disease.

关键词： nerve regeneration Trillium tschonoskii maxim homocysteine Alzheimer's disease cognitive disorders Tau synaptic development neural regeneration

Microstructure evolution and tensile mechanical properties of thixoformed AZ61 magnesium alloy prepared by squeeze casting

在线全文

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2014年第11期24卷 3421-3428页

作者：陈添解志文罗荘竹杨钦谭生王赟姣罗一旻中国科学院重庆绿色智能技术研究院重庆401122

A novel process that combines squeeze casting with partial remelting to obtain AZ61 magnesium alloy with semi-solid microstructures was proposed. In this route, the squeeze casting was used to predeform the magnesium alloy billets to obtain small dendritic structures. During subsequent partial remelting, small dendritic structures transform into globular grains surrounded by liquid films. The results show that the squeeze casting AZ61 alloy after partial remelting produces more ideal, finer semi-solid microstructure compared with as-cast AZ61 alloy treated by the same isothermal holding conditions. Moreover, the mechanical properties of the thixoformed AZ61 alloy prepared by squeeze casting plus partial remelting are better than those of the thixoformed alloy prepared by conventional casting plus partial remelting.

关键词： AZ61 magnesium alloy squeeze casting partial remelting mechanical properties

哈萨克族和汉族孕妇晚期妊娠可溶性血管内皮生长因子受体l与子痫前期的关系研究

在线全文

学校读者我要写书评

暂无评论

中国全科医学 2017年第28期20卷 3527-3531页

作者：张丽宏王志梅谢文娟朱启英新疆医科大学第一附属医院产科新疆乌鲁木齐市830054

目的探讨哈萨克族和汉族孕妇晚期妊娠可溶性血管内皮生长因子受体1(s Flt-1)与子痫前期(PE)的关系。方法选取2015年12月—2016年4月在新疆医科大学第一附属医院产科住院并分娩的孕妇110例,其中哈萨克族PE孕妇25例(哈萨克族PE组)、汉族P... 详细信息

目的探讨哈萨克族和汉族孕妇晚期妊娠可溶性血管内皮生长因子受体1(s Flt-1)与子痫前期(PE)的关系。方法选取2015年12月—2016年4月在新疆医科大学第一附属医院产科住院并分娩的孕妇110例,其中哈萨克族PE孕妇25例(哈萨克族PE组)、汉族PE孕妇25例(汉族PE组)、哈萨克族正常妊娠孕妇30例(哈萨克族正常妊娠组)、汉族正常妊娠孕妇30例(汉族正常妊娠组)。收集4组孕妇的临床资料,采用定量反转录聚合酶联反应(qRT-PCR)测定胎盘组织中的sFlt-1表达水平。结果哈萨克族PE组孕妇体质量高于汉族正常妊娠组,差异有统计学意义(P0.05)。哈萨克族重度PE、汉族重度PE、哈萨克族轻度PE、汉族轻度PE孕妇sFlt-1表达水平比较,差异无统计学意义(P>0.05)。结论晚期妊娠s Flt-1可能参与了PE的发生、发展,可作为预测PE发生、发展的血液学指标,但不能作为预测PE患者病情严重程度的指标。

关键词：子痫血管内皮生长因子受体1 妊娠末期哈萨克族汉族

Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 265-276页

作者： Yao Yuan Lingqi Yu Xudong Zhuang Dongjing wen Jin He Jingmei Hong Jiayu xie Shengan Ling Xiaoyue Du wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

Cerebral fat embolism following autologous fat injection in facial reconstruction:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2025年第2期13卷 50-58页

作者： Xiu-Ying Chen Fa Shen Chang Cheng Yu-Han Wang wen-Chao Cheng De-zhi Yuan wen Huang Department of Neurology Chongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400000China Department of Neurology Xinqiao HospitalThe Army Medical University(Third Military Medical University)Chongqing 400000China

BACKGROUND Autologous fat injection in facial reconstruction is a common cosmetic *** cerebral fat embolism(CFE)as a complication is rare,it carries serious health *** SUMMARY We present a case of a 29-year-old female patient who developed acute CFE following facial fat filling *** the surgery,the patient experienced symptoms including headache,nausea,vomiting,and difficulty breathing,which was followed by neurological symptoms such as slurred speech and left-sided *** physical examination and auxiliary investigations,including blood tests,head and neck computed tomography angiography,and cranial magnetic resonance diffusion-weighted imaging,were performed upon *** clinical diagnosis was acute cerebral embolism following facial fat filling *** included measures to improve cerebral circulation,dehydration for intracranial pressure reduction,nutritional support,and rehabilitation therapy for left limb *** patient showed a significant improvement in symptoms after 2 weeks of *** recovered left limb muscle strength to grade 5,had clear speech,and experienced complete relief of *** Our case highlights the potential occurrence of severe complications in patients undergoing fat injection in facial *** prevent these complications,plastic surgeons should enhance their professional knowledge and skills.

关键词： Cerebral fat embolism Mechanical thrombectomy Thrombolysis Steroid therapy Symptomatic treatment Case report

二陈汤加味对COPD大鼠转化生长因子-β_1,组蛋白去乙酰化酶2基因表达的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国实验方剂学杂志 2017年第10期23卷 147-154页

作者：陈四清季书尚立芝谢文英张良芝刘坦张静胡文豪张淼王祎河南中医药大学基础医学院郑州450046

目的:观察二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠肺组织中转化生长因子-β_1(TGF-β_1)及其受体(TGF-βRII)基因表达,外周血单个核细胞(PBMCs)中组蛋白去乙酰化酶2(HDAC2)基因表达及活性的影响。方法:采用烟熏加脂多糖方法制备COPD大... 详细信息

目的:观察二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠肺组织中转化生长因子-β_1(TGF-β_1)及其受体(TGF-βRII)基因表达,外周血单个核细胞(PBMCs)中组蛋白去乙酰化酶2(HDAC2)基因表达及活性的影响。方法:采用烟熏加脂多糖方法制备COPD大鼠模型。随机分为正常组、模型组、二陈汤加味高、中、低剂量组(20,10,5 g·kg^(-1))。正常组、模型组灌胃生理盐水(10 g·kg^(-1)),二陈汤加味高、中、低剂量组分别灌胃,连续14 d。荧光酶联免疫法测定PBMCs中HDAC2活性,酶联免疫吸附测定(ELISA)法测定肺组织匀浆中TGF-β_1及PBMCs中HDAC2含量;实时荧光定量PCR(Real-time PCR)检测肺组织匀浆中TGF-β_1mRNA及PBMCs中HDAC2 mRNA表达;免疫组化检测TGF-β_1与TGF-βRⅡ蛋白在肺组织的原位表达,光镜观察组织结构。结果:与正常组比较,模型组大鼠肺组织匀浆中TGF-β_1含量升高(P<0.05);肺组织中TGF-β_1mRNA,TGF-β_1与TGF-βRⅡ蛋白表达均显著增强(P<0.01);模型组大鼠PBMCs中HDAC2 mRNA表达、含量及其活性均明显减低(P<0.05,P<0.01),差异均有统计学意义。与模型组比较,二陈汤加味高、中剂量组肺组织匀浆中TGF-β_1mRNA表达(P<0.01)和TGF-β_1蛋白含量均显著降低(P<0.05,P<0.01),免疫组化检测肺组织中TGF-β_1与TGF-βRⅡ蛋白表达均显著弱于模型组(P<0.01),PBMCs中HDAC2 mRNA表达、含量及其活性均升高(P<0.05,P<0.01),肺组织结构明显改善。结论:二陈汤加味对COPD有抗炎作用。其机制可能与增强PBMCs中HDAC2基因表达,提高HDAC2活性,抑制TGF-β_1及其受体基因的表达有关。

关键词：慢性阻塞性肺疾病肺功能二陈汤加味转化生长因子-β1(TGF-β1) 组蛋白去乙酰化酶2

在线全文

学校读者我要写书评

暂无评论

腹水浓缩回输术治疗肝硬化腹水42例的护理

寄生虫病与感染性疾病 2004年第3期2卷 111-113页

作者：朱清文周立智谢文乐山市人民医院四川乐山614000

目的　总结腹水超滤浓缩回输腹腔治疗肝硬化顽固性腹水的护理要点及注意事项。方法　对 42例肝硬化顽固性腹水患者行腹水超滤浓缩回输腹腔治疗 ,治疗前后进行心理护理 ,严格无菌技术操作 ,保持管路通畅 ,密切观察病情 ,预防并发症等护... 详细信息

目的　总结腹水超滤浓缩回输腹腔治疗肝硬化顽固性腹水的护理要点及注意事项。方法　对 42例肝硬化顽固性腹水患者行腹水超滤浓缩回输腹腔治疗 ,治疗前后进行心理护理 ,严格无菌技术操作 ,保持管路通畅 ,密切观察病情 ,预防并发症等护理措施。结果　 42例患者均顺利完成治疗 ,治疗前后症状、腹围、尿量、血浆白蛋白及腹水蛋白都有显著性差异 ,自觉症状明显减轻 ,无护理并发症发生。结论　应用腹水超滤浓缩回输腹腔治疗肝硬化顽固性腹水是一种安全有效的方法 ,加强术前、术中、术后护理是保证治疗顺利进行并获得良好疗效的重要条件。

关键词：肝硬化顽固性腹水超滤浓缩回输护理

Design and Simulation Test of Advanced Secondary Cooling Control System of Continuous Casting Based on Fuzzy Self-Adaptive PID

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2011年第1期18卷 26-30页

作者： LIU wen-hong xie zhi Key Laboratory of Integrated Automation of Process Industry of Ministry of Education Northeastern University Shenyang 110819 Liaoning China College of Information Science and Engineering Northeastern University Shenyang 110819 Liaoning China

To improve billet quality and the trackability and stability of secondary cooling water during continuous casting, the superheat is introduced into the water distribution for secondary cooling to design the relevant control system, based on the water distribution model, superheat and fuzzy self-adaptive PID （process identity） . A spray cooling system is set up for simulation test in laboratory to test the step signal from the conventional, integral sepa rated and fuzzy self-adaptive PID controllers and the simulation casting. And the on-site test is done in some steel plant. The test results show that the fuzzy self-adaptive PID controller＇s performance is better than that of the other two controllers, which provides a basis for further study and application.

关键词： fuzzy self-adaptive PID continuous casting secondary cooling superheat

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

在线全文

学校读者我要写书评

暂无评论

Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang zhi-Lai Zhan Guang-Lu Chang Yan Gao Xia Li wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation