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南方林业科学 2019年第5期47卷 62-66页

作者：朱义林楼浙辉杨宸张亚萍张振洲朱宗威张华菊熊国辉靖安县林业局江西靖安330600 江西省林业科学院江西南昌330013

靖安县科学经营杉木骨干示范林项目建设,营建示范林53.5 hm2,实施杉木管护良法,取得了超预期的效果,制作了项目光盘。旨在传播杉木管护良法,提升森林质量,增加林农收入。

关键词：杉木管护良法骨干示范林

Total glucosides of Rhizoma Smilacis Glabrae:a therapeutic approach for psoriasis by regulating Th17/Treg balance

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Chinese Journal of Natural Medicines 2023年第8期21卷 589-598页

作者： TANG Yingzhan YU Jingyi ZHAO Wen LIU Juyan PENG Hongying zhang Haoran JIANG zhenzhou YU Qinwei zhang Luyong New drug screening center Jiangsu Center for Pharmacodynamics Research and EvaluationChina Pharmaceutical UniversityNanjing 210009China Guangzhou Baiyunshan Jingxiutang Pharmaceutical Co. Ltd.Guangzhou 510130China Guangzhou Pharmaceutical Holdings Co. Ltd.Guangzhou 510103China Center for Drug Research and Development Guangdong Pharmaceutical UniversityGuangzhou 510006China

Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T *** this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material *** findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis *** improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and *** transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular ***,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase ***,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial *** particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT *** summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg *** findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.

关键词： Psoriasis Rhizoma Smilacis Glabrae Th17/Treg balance Inflammation Epithelium development

抗菌肽Temprine-La(FS)抑制2型猪链球菌生物被膜形成的研究

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中国畜牧兽医 2021年第1期48卷 324-337页

作者：张欣潘晨浩赵瑞利姜轩金天明赵洋马吉飞于恩远李留安张振洲李桂霞李瑞忠天津农学院动物科学与动物医学学院天津300384 石家庄飞龙饲料有限公司石家庄050073

试验旨在研究抗菌肽Temprine-La(S)(T-La(S))、Temprine-La(FS)(T-La(FS))、RGD-T-La(S)和RGD-T-La(FS)对2型猪链球菌(SS2)生物被膜形成的抑制作用。通过结晶紫染色法(CV)检测SS2生物被膜形成能力;微量稀释法测定抗菌肽对SS2生物被膜的最小生物被膜抑菌浓度(MBIC)和最小生物被膜杀菌浓度(MBEC);结晶紫染色法和扫描电镜(SEM)检测抗菌肽对SS2生物被膜形成的影响;XTT法检测抗菌肽对SS2生物被膜代谢活性的影响;苯酚硫酸法检测抗菌肽对SS2生物被膜胞外多糖含量的影响;建立猪链球菌-斑马鱼感染模型,HE染色法观察T-La(FS)对斑马鱼脑组织病理变化的影响;实时荧光定量PCR法分析抗菌肽对SS2生物被膜相关基因及对斑马鱼炎性细胞因子基因转录水平的影响。结果显示,SS2具有良好的生物被膜形成能力;T-La(S)、RGD-T-La(S)、T-La(FS)和RGD-T-La(FS)对SS2生物被膜的MBIC分别为31.3、15.6、7.8和15.6μg/mL;MBEC分别为62.6、31.2、15.6和31.2μg/mL;结晶紫染色结果表明,抗菌肽对SS2生物被膜的形成有抑制作用;扫描电镜结果显示,抗菌肽使SS2生物被膜中的细菌数量和生物被膜的形态发生明显变化,细胞外基质大量减少;XTT法结果显示,抗菌肽可显著降低SS2生物被膜的代谢活性;苯酚硫酸法结果显示,抗菌肽能有效抑制SS2生物被膜合成胞外多糖;实时荧光定量PCR结果表明,抗菌肽作用后降低了SS2生物被膜基因的转录水平;T-La(FS)作用后TLR2、MyD88及促炎性细胞因子基因的转录水平显著或极显著降低(P<0.05;P<0.01),抗炎性细胞因子基因的转录水平显著或极显著升高(P<0.05;P<0.01)。抗菌肽主要通过影响生物被膜相关基因转录水平和阻断胞外多糖的合成与分泌来抑制SS2生物被膜的形成,其中T-La(FS)可能通过抑制TLR2信号通路中TLR2和MyD88分子表达,抑制炎性细胞因子的释放,减轻脑膜炎的炎性反应。

关键词：抗菌肽Temprine-La(FS) 2型猪链球菌生物被膜斑马鱼 TLR2 MyD88

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硫酸锌浸种对马铃薯产量和品质的影响研究

中国马铃薯 2010年第1期24卷 18-21页

作者：贾景丽张振洲周芳赵娜刘兆才黄瑞冬沈阳农业大学农学院辽宁沈阳110161 辽宁省本溪市马铃薯研究所辽宁本溪117000

田间条件下,通过不同浓度硫酸锌浸种,研究锌对马铃薯生长发育、产量及品质的影响。结果表明:适量浓度硫酸锌浸种能够促进马铃薯植株生长发育、增加产量、提升品质;但锌素过多又会产生不利影响。其中0.05%浓度硫酸锌浸种植株生长旺盛,产... 详细信息

田间条件下,通过不同浓度硫酸锌浸种,研究锌对马铃薯生长发育、产量及品质的影响。结果表明:适量浓度硫酸锌浸种能够促进马铃薯植株生长发育、增加产量、提升品质;但锌素过多又会产生不利影响。其中0.05%浓度硫酸锌浸种植株生长旺盛,产量增加显著;0.10%浓度硫酸锌浸种块茎食用品质和商品品质最佳。因而,在本试验土壤条件下,用0.05%～0.10%浓度的硫酸锌浸种能够有效地提高马铃薯产量、提升食用品质和商品品质。

关键词：马铃薯硫酸锌产量品质

抗菌肽Temprine-La(FS)抑制耐甲氧西林伪中间型葡萄球菌生物被膜形成的研究

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天津农学院学报 2021年第1期28卷 24-33页

作者：张欣潘晨浩赵瑞利姜轩赵洋马吉飞于恩远李留安张振洲天津农学院动物科学与动物医学学院天津300392

为了探究抗菌肽Temprine-La(FS)对耐甲氧西林伪中间型葡萄球菌(MRSP)生物被膜形成的影响,本试验以Temprine-La为基序进行氨基酸改造合成新的抗菌肽T-La(S)、T-La(FS)、RGD-T-La(S)、RGD-T-La(FS)以提高抗菌活性。通过微量稀释法测定多肽... 详细信息

为了探究抗菌肽Temprine-La(FS)对耐甲氧西林伪中间型葡萄球菌(MRSP)生物被膜形成的影响,本试验以Temprine-La为基序进行氨基酸改造合成新的抗菌肽T-La(S)、T-La(FS)、RGD-T-La(S)、RGD-T-La(FS)以提高抗菌活性。通过微量稀释法测定多肽对MRSP生物被膜的最小抑菌浓度(MBIC);结晶紫染色法(CV)检测多肽对MRSP生物被膜形成能力的影响;XTT法检测多肽对MRSP生物被膜代谢活性的影响、多肽对MRSP生物被膜胞外多糖含量的影响;扫描电镜(SEM)检测多肽对MRSP生物被膜形成的影响;荧光定量PCR法(q-PCR)分析多肽对MRSP生物被膜相关基因转录水平的影响。结果显示,T-La(S)、RGD-T-La(S)、T-La(FS)、RGD-T-La(FS)对MRSP的MBIC分别为:31.3、15.6、7.8、15.6μg/mL;结晶紫染色法(CV)表明T-La(S)、RGD-T-La(S)、T-La(FS)、RGD-T-La(FS)对MRSP生物被膜形成有抑制作用;扫描电镜(SEM)结果显示多肽使MRSP生物被膜中细胞外基质大量减少,菌体裸露在物体表面;多肽可以显著降低MRSP生物被膜的代谢活性,抑制被膜菌的粘附作用;多肽能有效抑制MRSP生物被膜合成胞外多糖;荧光定量PCR结果表明,多肽作用后能够降低MRSP生物被膜相关基因的转录水平。因此,多肽主要通过影响生物被膜相关基因转录水平和阻断胞外多糖的合成与分泌来抑制MRSP生物被膜的形成。

关键词：抗菌肽Temprine-La(FS) 耐甲氧西林伪中间型葡萄球菌(MRSP) 生物被膜相关基因

X-ray scintillation in lead-free double perovskite crystals

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Science China Chemistry 2018年第12期61卷 1581-1586页

作者： Qingsong Hu zhenzhou Deng Manchen Hu Anjiang Zhao Yaqi zhang Zhifang Tan Guangda Niu Haodi Wu Jiang Tang Wuhan National Laboratory for Optoelectronics(WNLO) Huazhong University of Science and Technology (HUST) School of Information Engineering Nanchang University School of Optical and Electronic Information Huazhong University of Science and Technology (HUST) College of Life Science and Technology Huazhong University of Science and Technology (HUST) College of Physics Huazhong University of Science and Technology (HUST)

Metal halide perovskites have shown great performance for various applications,including solar cells,light emitting diodes,and radiation detectors,but they still suffer from the toxicity of lead and *** we report the use of lanthanide series as trivalent metals to obtain low toxicity and highly stable double perovskites(Cs_2NaLnCl_6,Ln=Tb or Eu)with high scintillation light *** crystals exhibit typical f-f transitions of lanthanide cations,while Cs_2NaTbCl_6exhibits strong green photoluminescence,and Cs_2NaEuCl_6exhibits red *** X-ray radiations,the light yield of Cs_2NaTbCl_6reaches46600 photons MeV^(-1),much higher than that of the commercially used(Lu,Y)_2SiO_5:Ce^(3+)crystals(LYSO,28500 photons MeV^(-1)),and previously reported lead-based perovskites(14000 photons MeV^(-1)).As a new member of lead-free perovskites,lanthanide-based double perovskites open up a new route toward radiation detections and potential medical imaging.

关键词： low toxic scintillation light yield lanthanide double perovskites

The CDF and its sensitivity analysis of stochastic structure with stochastic excitation by advanced stratified line sampling

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Science China(Physics,Mechanics & Astronomy) 2013年第8期56卷 1559-1567页

作者： SONG ShuFang LU zhenzhou zhang WeiWeiNational Key Laboratory of Aerodynamic Design and Research Northwestern Polytechnical University YE ZhengYin School of Aeronautics Northwestern Polytechnical University National Key Laboratory of Aerodynamic Design and Research Northwestern Polytechnical University

For the stochastic structure with stochastic excitation, an advanced stratified line sampling (SLS) method is presented to obtain the cumulative distribution function (CDF) of the structural response and its sensitivity. The advanced stratified line sampling method introduces a set of middle failure subsets firstly. And for each subset, the conventional line sampling can be used to obtain the corresponding value of the response's CDF. At the same time, the sensitivity estimations of each failure subset can also be computed by modifying the important direction and corresponding reliability coefficients. The properties of CDF sensitivity are proved while the performance function is linear with normal random variables. After two simple examples are used to demonstrate the properties of CDF sensitivity and the feasibility of the presented method, the method employed to analyze the CDF and corresponding sensitivity of root bending moment (RBM) responses for the stochastic BAH is wing with gust excitation to a square-edged gust and to a Dryden gust. The results show that the parameters of the second and the fifth order modals exert more influence on the CDF of response than the other ones, and the presented SLS method can more significantly reduce the computational cost compared with Monte Carlo simulation (MCS).

关键词： stochastic excitation line sampling cumulative distribution function (CDF) sensitivity analysis root bending moment (RBM) gust response analysis

Reliability and Sensitivity Analysis of Transonic Flutter Using Improved Line Sampling Technique

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Chinese Journal of Aeronautics 2009年第5期22卷 513-519页

作者： Song Shufang Lu zhenzhou zhang Weiwei Ye Zhengyin School of Aeronautics Northwestern Polytechnical University Xi'an 710072 China National Key Laboratory of Aerodynamic Design and Research Northwestern Polytechnical University Xi'an 710072 China

The improved line sampling （LS） technique, an effective numerical simulation method, is employed to analyze the probabilistic characteristics and reliability sensitivity of flutter with random structural parameter in transonic flow. The improved LS technique is a novel methodology for reliability and sensitivity analysis of high dimensionality and low probability problem with implicit limit state function, and it does not require any approximating surrogate of the implicit limit state equation. The improved LS is used to estimate the flutter reliability and the sensitivity of a two-dimensional wing, in which some structural properties, such as frequency, parameters of gravity center and mass ratio, are considered as random variables. Computational fluid dynamics （CFD） based unsteady aerodynamic reduced order model （ROM） method is used to construct the aerodynamic state equations. Coupling structural state equations with aerodynamic state equations, the safety margin of flutter is founded by using the critical velocity of flutter. The results show that the improved LS technique can effectively decrease the computational cost in the random uncertainty analysis of flutter. The reliability sensitivity, defined by the partial derivative of the failure probability with respect to the distribution parameter of random variable, can help to identify the important parameters and guide the structural optimization design.

关键词： flutter aeroelastic line sampling technique Monte Carlo simulation uncertainty reduced order model

A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses

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Virologica Sinica 2021年第4期36卷 746-754页

作者： Yingxue Li zhenzhou Wan Lulu Zuo Shenwei Li Honglian Liu Yingying Ma Lianqun Zhou Xia Jin Yuye Li Chiyu zhang Shanghai Public Health Clinical Center Fudan UniversityShanghai201508China CAS Key Laboratory of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou215163China School of Biomedical Engineering University of Science and Technology of ChinaHefei260026China Medical Laboratory of Taizhou Fourth People's Hospital Taizhou225300China Viral Hemorrhagic Fevers Research Unit Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai200031China Shanghai International Travel Healthcare Center Shanghai200335China Department of Dermatology and Venereology First Affiliated Hospital of Kunming Medical UniversityKunming650032China

Human herpesviruses are double-stranded DNA viruses that are classified into nine *** than 90%of adults are ever infected with one or more *** symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and *** and accurate detection of herpesvirus infection is critical for clinical management and *** this study,we established a single-tube nonuple qPCR assay for detection of all nine herpesviruses using a 2-D multiplex qPCR method with a house-keeping gene as the internal *** novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25µL single-tube reaction,and does not cross-react with 20 other human viruses,including DNA and RNA *** robustness of the novel assay was evaluated using 170 clinical *** novel assay showed a high consistency(100%)with the single qPCR assay for HHVs *** features of simple,rapid,high sensitivity,specificity,and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.

关键词： Human herpesviruses(HHVs) 2-D multiplex qPCR Melting temperature(Tm) Fluorescence probe Melting curve

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

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Virologica Sinica 2020年第3期35卷 344-347页

作者： Renfei Lu Xiuming Wu zhenzhou Wan Yingxue Li Lulu Zuo Jianru Qin Xia Jin Chiyu zhang Clinical Laboratory Nantong Third Hospital Affiliated to Nantong UniversityNantong 226006China Pathogen Discovery and Evolution Unit Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China Medical Laboratory of Taizhou Fourth People’s Hospital Taizhou 225300China College of Life Sciences Henan Normal UniversityXinxiang 453007China Shanghai Public Health Clinical Center Fudan UniversityJinshan DistrictShanghai 201508China

Dear Editor,Since early December 2019, a large outbreak of pneumonia caused by a novel coronavirus(COVID-19) had emerged in Wuhan, China(Wu et al. 2020 a, b;Zhou et al. 2020;Zhu et al. 2020;Jiang and Shi 2020). Similar to severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome coronavirus(MERS-CoV),the new coronavirus also belongs to Betacoronavirus。

关键词： al. respiratory acute