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Frontiers of Architectural Research 2023年第3期12卷 556-574页

作者： Wenfang He zhenying wu Ran Jin Jiaping Liu School of Architecture Xi’an University of Architecture and TechnologyXi’an 710055China State Key Laboratory of Green Building in Western China Xi’an University of Architecture and TechnologyXi’an 710055China

Under the global crisis of energy shortage and environmental pollution,the climate responsive strategies used in vernacular buildings have attracted much attention for their potential to reduce energy consumption and carbon emissions.However,the relationships between these traditional climatic strategies were not precisely perceived,which may cause the inapplicability of these strategies for contemporary rural houses.In this paper,taking the Turpan vernacular buildings in arid region of China,the climate responsive strategies of buildings in the most significant periods such as the Gaochang period(before 1318),the Khanate and Republican period(1318–1949),the Modern period(1949–2010),and the Contemporary period(2011–present)were summarized.In addition,two different types of climatic strategies organizations,namely multilayer spaces and integrated building envelopes,were identified based on the temperature difference measurement and comparative analysis.The assessment of thermal performance of the organizations was conducted by the methodology of software simulation.Furthermore,the applicability of the organizations in rural areas was discussed,and a new combined organization was proposed.Consequently,this study can contribute to provide the main approaches for climatically responsive rural houses.

关键词： Climate responsive strategy Vernacular building Organization Temperature difference Multilayer space Arid

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幼儿园生态游戏场的建构及其活动实施

学前教育研究 2024年第1期 87-90页

作者：吴桢映浙江省慈溪市实验幼儿园教育集团慈溪315300

遵从自然规律的教育可以更好地塑造完整的儿童。以生态为基本视域,将幼儿的学习场分为自然、生活和生产,强调幼儿学习的主体性、探究性和愉悦性,是幼儿自然教育的应有之义。幼儿园可以通过建构自然游戏场、生活游戏场和生产游戏场的方... 详细信息

遵从自然规律的教育可以更好地塑造完整的儿童。以生态为基本视域,将幼儿的学习场分为自然、生活和生产,强调幼儿学习的主体性、探究性和愉悦性,是幼儿自然教育的应有之义。幼儿园可以通过建构自然游戏场、生活游戏场和生产游戏场的方式来为幼儿创设完整的学习环境,通过情景体验、任务驱动和问题探究的方式来促进幼儿的深度学习。

关键词：生态游戏场自然教育自主探究园本课程

On Power Finite Rank Operators

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Journal of Mathematical Research with Applications 2019年第4期39卷 378-382页

作者： Qingping ZENG zhenying wu College of Computer and Information Sciences Fujian Agriculture and Forestry University College of Mathematics and Informatics Fujian Normal University

An operator F ∈ B（X） is called power finite rank if F;is of finite rank for some n ∈ N.In this note, we provide several interesting characterizations of power finite rank operators. In particular, we show that the class of power finite rank operators is the intersection of the class of Riesz operators and the class of operators with eventual topological uniform descent.

关键词： power finite rank operator Drazin invertible eventual topological uniform descent Riesz operator

小桐子植物特异性酪蛋白激酶PS-CK1-5基因的克隆及原核表达分析

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热带亚热带植物学报 2024年第1期32卷 93-100页

作者：王海波吴贞莹郭俊云曲靖师范学院生物资源与食品工程学院云南曲靖655011

酪蛋白激酶(casein kinase,CK)作为一类普遍存在的Ser/Thr蛋白激酶,通过调节靶标蛋白的活性与稳定性,在植物整个生理过程及信号转导途径中发挥重要作用。基于同源序列比对,该研究对小桐子(Jatropha curcas)酪蛋白激酶基因家族进行鉴定... 详细信息

酪蛋白激酶(casein kinase,CK)作为一类普遍存在的Ser/Thr蛋白激酶,通过调节靶标蛋白的活性与稳定性,在植物整个生理过程及信号转导途径中发挥重要作用。基于同源序列比对,该研究对小桐子(Jatropha curcas)酪蛋白激酶基因家族进行鉴定与表达分析。结果表明,小桐子基因组中共鉴定到7个酪蛋白激酶1基因(CK1)、5个植物特异性酪蛋白激酶1基因(PS-CK1)、3个酪蛋白激酶2α亚基基因(CK2-α)、2个酪蛋白激酶2β亚基基因(CK2-β),4个亚家族成员在氨基酸长度、等电点及外显子数目等都有其家族特异性。蛋白的氨基酸序列比对表明,小桐子酪蛋白激酶1都包含N端保守激酶结构域,同时其内部都鉴定到典型的激酶活性环基序、ATP结合核心基序、核定位信号肽。qRT-PCR表达分析表明,小桐子JcPS-CK1-5基因在叶片与根中都属于低温诱导基因,可能参与小桐子抗冷性过程。构建其原核表达载体pET-32a-JcPS-CK1-5,并在BL21(DE3)中诱导表达,得到81.6 kD的条带,与理论融合蛋白的分子量一致。这可为小桐子CK基因的功能鉴定及逆境信号转导机制研究提供参考。

关键词：小桐子基因家族酪蛋白激酶基因克隆原核表达

Functional characterization of caffeic acid O-methyltransferase in internode lignification of switchgrass(Panicum virgatum)

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Frontiers of Agricultural Science and Engineering 2018年第1期5卷 98-107页

作者： Fengyan wu zhenying wu Aiguo YANG Shanshan JIANG Zeng-Yu WANG Chunxiang FU Key Laboratory of Energy Genetics of Shandong Province/Qingdao Engineering Research Center of Biomass Resources and Environment/Key Laboratory of Biofuels/Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Tobacco Research Institute Chinese Academy of Agricultural Sciences Forage Improvement Division The Samuel Roberts Noble Foundation

Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin.

关键词： biofuel crop caffeic acid O-methyltransferase forage lignin Panicum virgatum switchgrass transgenic plant

锡掺杂对氧化锌纳米柱阵列结构与光电性能的影响

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功能材料与器件学报 2024年第2期 74-78页

作者：汤小兰吴振英苏州工业职业技术学院电子与通信工程系江苏苏州215104

选择不同的Sn浓度,以溶胶凝胶法在N型重掺杂基板上制备了系列Zn_(1-x)Sn_(x)O(x=0.01-0.07)纳米柱样品,并在不同温度下(500℃、600℃、700℃)进行了退火热处理。利用光/暗电流测试对样品的光电性能进行了表征,发现当偏压为5V时,在600℃... 详细信息

选择不同的Sn浓度,以溶胶凝胶法在N型重掺杂基板上制备了系列Zn_(1-x)Sn_(x)O(x=0.01-0.07)纳米柱样品,并在不同温度下(500℃、600℃、700℃)进行了退火热处理。利用光/暗电流测试对样品的光电性能进行了表征,发现当偏压为5V时,在600℃退火1小时后,x=0.04的样品表现出最佳的光/暗电流变化比(600%)。XRD与FE-SEM结果显示该纳米柱样品具有最大的长度以及较好的结晶度,意味着样品具有较高的结晶质量;PL测试发现x=0.04时样品缺陷浓度最小,这说明在该缺陷浓度下锡对锌进行替代时产生了更多的载流子,从而导致了最优的光电性能。

关键词：锡掺杂氧化锌光电性能溶胶凝胶法

MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar

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Horticulture Research 2020年第1期7卷 1247-1258页

作者： Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang zhenying wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu Shandong Provincial Key Laboratory of Energy Genetics Key Laboratory of BiofuelsChinese Academy of SciencesQingdao Institute of BioEnergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China University of Chinese Academy of Sciences Beijing 100049China College of Biological Sciences&Biotechnology Beijing Forestry UniversityBeijing 10083China Collaborative Innovation Center of Crop Stress Biology Henan Province and Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences HarbinHeilongjiangChina

Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.

关键词： miR156 analysis anthocyanin

蒙古冰草咖啡酸氧甲基转移酶基因AmCOMT1的鉴定及功能分析

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植物学报 2024年第3期59卷 383-396页

作者：杜锦瑜孙震苏彦龙王贺萍刘亚玲吴振映何峰赵彦付春祥内蒙古农业大学草原与资源环境学院/农业农村部饲草栽培、加工与高效利用重点实验室/草地资源教育部重点实验室呼和浩特010018 中国科学院青岛生物能源与过程研究所青岛266101 山东能源研究院青岛266101 青岛新能源山东省实验室青岛266101 内蒙古草业技术创新中心有限公司呼和浩特010018

蒙古冰草(即沙芦草(Agropyron mongolicum))是我国北方代表性的多年生牧草之一,具有较强的耐寒和耐旱能力。在植物中,咖啡酸氧甲基转移酶基因(COMT)是参与木质素和褪黑素生物合成的关键基因,在调节植物生长、品质和抗逆性中发挥重要作... 详细信息

蒙古冰草(即沙芦草(Agropyron mongolicum))是我国北方代表性的多年生牧草之一,具有较强的耐寒和耐旱能力。在植物中,咖啡酸氧甲基转移酶基因(COMT)是参与木质素和褪黑素生物合成的关键基因,在调节植物生长、品质和抗逆性中发挥重要作用。通过分析蒙古冰草全长转录组数据,从蒙古冰草中克隆了COMT候选基因AmCOMT1。该基因在茎秆和根等木质素含量高的组织中高表达,且其表达受多种非生物胁迫诱导。在拟南芥(Arabidopsis thaliana)野生型(Col-0)和突变体(omt1-2)中过表达AmCOMT1,显著促进了转基因拟南芥的木质素合成,使突变体的木质素单体和组分恢复至野生型水平,同时Col-0/35S:AmCOMT1中木质素总量提高11%。此外, AmCOMT1过表达显著提高了Col-0/35S:AmCOMT1转基因拟南芥的褪黑素含量。在盐胁迫条件下,该株系平均根长相比野生型拟南芥提高20.3%,表现出更强的抗逆性。综上,蒙古冰草AmCOMT1基因在木质素和褪黑素合成中发挥关键作用,可提高转基因拟南芥的抗逆性,在蒙古冰草等单子叶牧草遗传改良方面具有重要应用潜力。

关键词：蒙古冰草咖啡酸氧甲基转移酶木质素褪黑素盐胁迫

Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height

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Horticulture Research 2021年第1期8卷 3506-3519页

作者： Ruijuan Yang zhenying wu Chen Bai Zhichao Sun Mengqi Wang Yuzhu Huo Hailing Zhang Yamei Wang Huapeng Zhou Shaojun Dai Wenwen Liu Chunxiang Fu Shandong Provincial Key Laboratory of Energy Genetics and CAS Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences266101 QingdaoShandongChina University of Chinese Academy of Sciences 100049 BeijingChina Shanghai Normal University 201418 ShanghaiChina Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences HarbinHeilongjiangChina Key Laboratory of Bio-resource and Eco-environment of Ministry of Education College of Life SciencesSichuan University610064 ChengduChina

Switchgrass(Panicum virgatum L.)is an important perennial,noninvasive,tall ornamental grass that adds color and texture to gardens and landscapes.Moreover,switchgrass has been considered a forage and bioenergy crop because of its vigorous growth,low-input requirements,and broad geography.Here,we identified PvWOX3a from switchgrass,which encodes a wuSCHEL-related homeobox transcription factor.Transgenic overexpression of PvWOX3a in switchgrass increased stem length,internode diameter,and leaf blade length and width,all of which contributed to a 95%average increase in dry weight biomass compared with control plants.Yeast one-hybrid and transient dual-luciferase assays showed that PvWOX3a can repress the expression of gibberellin 2-oxidase and cytokinin oxidase/dehydrogenase through apparently direct interaction with their promoter sequences.These results suggested that overexpression of PvWOX3a could increase gibberellin and cytokinin levels in transgenic switchgrass plants,which promotes cell division,elongation,and vascular bundle development.We also overexpressed PvWOX3a in a transgenic miR156-overexpressing switchgrass line that characteristically exhibited more tillers,thinner internodes,and narrower leaf blades.Double transgenic switchgrass plants displayed significant increases in internode length and diameter,leaf blade width,and plant height but retained a tiller number comparable to that of plants expressing miR156 alone.Ultimately,the double transgenic switchgrass plants produced 174%more dry-weight biomass and 162%more solubilized sugars on average than control plants.These findings indicated that PvWOX3a is a viable potential genetic target for engineering improved shoot architecture and biomass yield of horticulture,fodder,and biofuel crops.

关键词： expressing blade landscape

Samuel multiplicity and the structure of essentially semi-regular operators: A note on a paper of Fang

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Science China Mathematics 2013年第6期56卷 1213-1231页

作者： ZENG QingPing ZHONG HuaiJie wu zhenying School of Mathematics and Computer Science Fujian Normal University Fuzhou Strait Vocation Technological College

Motivated by a paper of Fang (2009), we study the Samuel multiplicity and the structure of essentially semi-regular operators on an infinite-dimensional complex Banach space. First, we generalize Fang's results concerning Samuel multiplicity from semi-Fredholm operators to essentially semi-regular operators by elementary methods in operator theory. Second, we study the structure of essentially semi-regular operators. More precisely, we present a revised version of Fang's 4 × 4 upper triangular model with a little modification, and prove it in detail after providing numerous preliminary results, some of which are inspired by Fang's paper. At last, as some applications, we get the structure of semi-Fredholm operators which revised Fang's 4 × 4 upper triangular model, from a different viewpoint, and characterize a semi-regular point λ∈ C in an essentially semi-regular domain.

关键词：空间结构半正规运营商多样性半Fredholm算子 Banach 三角模型初等方法