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Identification and Characterization of a New IgE-binding Protein in Mackerel (Scomber japonicus) by MALDI-TOF-MS

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Journal of Ocean University of China 2011年第1期10卷 93-98页

作者： WANG Bangping LI zhenxing zheng Lina LIU Yixuan LIN Hong Food Safety Laboratory College of Food Science and Engineering Ocean University of China Qingdao 266003 P. R. China

As fish is one source of the ‘big eight’ food allergens,the prevalence of fish allergy has increased over the past few *** order to better understand fish allergy,it is necessary to identify fish *** on the sera from fish-allergenic patients,a 28 kDa protein from local mackerel (Scomber japonicus),which has not been reported as a fish allergen,was found to be reactive with most of the patients’ *** 28 kDa protein was analyzed by MALDI-TOF-MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry).Mascot search in NCBI database (Date: 08/07/2010) showed that the top protein matched,*** isomerase (TPI) from Xiphophorus maculatus and Poecilia reticulata,had a mowse (molecular weight search) score of *** addition,TPI from Epinephelus coioides also matched this mackerel protein with a mowse score of *** TPI is con-sidered as an allergen in other non-fish organisms,such as lychee,wheat,latex,archaeopotamobius (Archaeopotamobius sibiriensis) and crangon (Crangon crangon),we consider that it may also be an allergen in mackerel.

关键词： mackerel (Scomberjaponicus) immunoblotting MALDI-TOF-MS allergen

翠碧一号不同部位物理特性及化学成分差异研究

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农产品加工 2024年第8期 71-74页

作者：郑振兴李科都丁佳冯永新李萌河北中烟工业有限责任公司河北石家庄050011 郑州轻工业大学烟草科学与工程学院河南郑州450001

以福建省龙岩市连城县标准化生产管理烟田烤后烟叶为试验材料,对样品化学成分、物理特性进行检测,采用描述性统计、变异系数分析、偏态峰和峰度分析及差异显著性分析等方法来分析研究翠碧一号烟叶不同部位的主要物理和化学特性。物理特... 详细信息

以福建省龙岩市连城县标准化生产管理烟田烤后烟叶为试验材料,对样品化学成分、物理特性进行检测,采用描述性统计、变异系数分析、偏态峰和峰度分析及差异显著性分析等方法来分析研究翠碧一号烟叶不同部位的主要物理和化学特性。物理特性分析表明,翠碧一号烟叶的厚度、含梗率、拉力、叶梗分离力、剪切力、穿透力和黏附力在3个不同部位间存在显著差异。上部烟叶在这些物理指标上普遍优于中部和下部烟叶。具体来说,上部烟叶的厚度比下部烟叶厚53.60μm,拉力高1.94 N,叶梗分离力高0.41N,剪切力高0.79 N,穿透力高0.50 N,黏附力高0.74 N。而含梗率以下部烟叶最高。化学成分分析显示,水溶性总糖、还原糖、烟碱、总氮和钾的含量在不同部位间也存在显著差异。中部烟叶的水溶性总糖和还原糖含量最高,下部烟叶次之,上部烟叶最低。烟碱和总氮含量则以上部烟叶最高,中部烟叶次之,下部烟叶最低。钾含量以下部烟叶最高,中部烟叶次之,上部烟叶最低。这些结果提供了翠碧一号烟叶不同部位特性的重要信息,为烟叶的分级和加工利用提供了科学依据。

关键词：翠碧一号物理特性化学成分

基于SP+模型的三元锂离子电池基本反应模型参数修正研究

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黑龙江科学 2024年第4期15卷 94-98页

作者：徐伟周显松许铀周开晴姚锦祥郑振兴广东技术师范大学广州510665

通过改进单粒子模型(SP+模型)对电池基本参数进行预测及限制负极电位来获得充电安全边界是快充策略应用的有效评估方法。SP+模型需建立在三电极电池的基础上,在各种特性上与真实电池有一定的区别,这导致利用负极电位评估方法在预测精度... 详细信息

通过改进单粒子模型(SP+模型)对电池基本参数进行预测及限制负极电位来获得充电安全边界是快充策略应用的有效评估方法。SP+模型需建立在三电极电池的基础上,在各种特性上与真实电池有一定的区别,这导致利用负极电位评估方法在预测精度上存在一定的差异。针对上述问题建立电池基本反应模型,将修正因子导入到涉及SOC、正负极电位、正负极嵌锂浓度的电池平衡电动势表达式中,设置相应的测试计划对修正因子进行标定,实现在25℃和特定SOC情况下对三元锂离子电池平衡电动势及正负极电势预测精度的提升,为后续安全边界评估开路正负极电位预测值提供参考。

关键词：三元锂离子动力电池改进单粒子模型(SP+模型) 电池基本反应模型

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烟草黑胫病和青枯病抗药性检测

生物灾害科学 2024年第1期47卷 111-118页

作者：李敏靳彦峰郑振兴安泉成陈雨轩王杰周艳宾河北中烟工业有限责任公司河北石家庄050000 中国农业科学院烟草研究所山东青岛266101

【目的】长期连续施用化学农药防治烟草根茎病害导致病原菌产生不同程度的抗药性。为明确病原菌的抗药性水平,根据烟草K326品种黑胫病和青枯病发生和流行的关键要素筛选对其有高效低毒低残留杀菌剂,建立精准施药技术。【方法】采集广西... 详细信息

【目的】长期连续施用化学农药防治烟草根茎病害导致病原菌产生不同程度的抗药性。为明确病原菌的抗药性水平,根据烟草K326品种黑胫病和青枯病发生和流行的关键要素筛选对其有高效低毒低残留杀菌剂,建立精准施药技术。【方法】采集广西壮族自治区主要烟区烟草黑胫病和青枯病病株,采用组织分离法分离得到黑胫病和青枯病的病原菌,通过生长速率法测得不同杀菌剂对病原菌的EC50,进而计算得到抗性指数。【结果】在广西烟区一共分离得到185株烟草黑胫病菌和79株烟草青枯病菌。其中,50%烯酰吗啉可湿性粉剂对烟草黑胫病菌的抑制效果最好,EC50仅为1.96μg/m L。而66.5%霜霉威盐酸盐水剂的抑制效果最差,EC50为8 863.03μg/m L。烟草黑胫病菌已对甲霜·锰锌、噁霉·稻瘟灵、甲基硫菌灵、多菌灵和霜霉威产生了高度抗性。52%氯尿硫酸铜可溶性粉剂对烟草青枯病菌的抑制效果最好,EC50达到了97.55μg/m L。而20%噻菌铜悬浮剂的抑制效果最差,EC50达到了198.25μg/mL。90%以上烟草青枯病原菌对6种药剂均敏感,没有产生明显的抗药性。【结论】广西烟区烯酰吗啉和氟吗·乙铝在生产防治上仍可选用。对其余5种药剂来说,所有分离出的烟草黑胫病菌均为高抗菌株,抗性频率达到了100%,这5种药剂在广西烟区烟草黑胫病的防控中已经失效,没有实用价值。对于烟草青枯病菌来说,噻森铜、氯尿硫酸铜、中生菌素、噻唑锌、溴菌壬菌铜和噻菌铜都以敏感菌株为优势群体,占比94.94%。靖西2株表现出高抗性,富川2株表现出中抗性,仅占总菌株数的5.06%,上述6种药剂仍可用于百色和贺州主要烟区防治烟草青枯病。

关键词：广西烟区烟草黑胫病烟草青枯病抗药性

3D vertically aligned microchannel structure to enhance piezoelectric energy harvesting performance of PZT/PVDF&CNTs piezoelectric composites

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Journal of Materiomics 2024年第5期10卷 1063-1070页

作者： Limin Hou Yu Huan Mupeng zheng Yilong Liu Chuilei Wang Xinjian Wang Changxiao Li zhenxing Wang Tao Wei School of Materials Science and Engineering University of JinanJinan250022China Key Laboratory of Advanced Functional Materials Faculty of Materials and ManufacturingBeijing University of TechnologyBeijing100124China Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education and International Center for Dielectric ResearchXi'an Jiaotong UniversityXi'an710049China SICC Co. LtdJinan250000China

Piezoelectric energy harvesters(PEHs)have attracted significant attention with the ability of converting mechanical energy into electrical energy and power the self-powered microelectronic ***,material's superior energy harvesting performance is closely related to its high transduction coefficient(d_(33)×g_(33)),which is dependent on higher piezoelectric coefficient d33 and lower dielectric constantεr of ***,the high d33 and lowεr are difficult to be simultaneously achieved in piezoelectric ***,lead zirconate titanate(PZT)based piezoelectric composites with vertically aligned microchannel structure are constructed by phase-inversion *** polyvinylidene fluoride(PVDF)and carbon nanotubes(CNTs)are mixed as fillers to fabricate PZT/PVDF&CNTs *** unique structure and uniformly distributed CNTs network enhance the polarization and thus improve the *** PVDF filler effectively reduce theε*** a consequence,the excellent piezoelectric coefficient(d_(33)=595 pC/N)and relatively low dielectric constant(ε_(r)=1,603)were obtained in PZT/PVDF&CNTs composites,which generated an ultra-high d_(33)×g_(33) of 24,942×10^(−15) m^(2)/***,the PZT/PVDF&CNTs piezoelectric composites achieve excellent energy harvesting performance(output voltage:66 V,short current:39.22μA,and power density:1.25μW/mm^(2)).Our strategy effectively boosts the performance of piezoelectric-polymer composites,which has certain guiding significance for design of energy harvesters.

关键词： Piezoelectric-polymer composites Phase-inversion method 3D vertically aligned microchannel structure Piezoelectric energy harvester Carbon nanotubes

Hundred-Joule-level,nanosecond-pulse Nd:glass laser system with high spatiotemporal beam quality

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High Power Laser Science and Engineering 2016年第1期4卷 63-74页

作者： Sensen Li Yulei Wang Zhiwei Lu Lei Ding Yi Chen Pengyuan Du Dexin Ba zhenxing zheng Xin Wang Hang Yuan Chengyu Zhu Weiming He Dianyang Lin Yongkang Dong Dengwang Zhou Zhenxu Bai Zhaohong Liu Can Cui National Key Laboratory of Science and Technology on Tunable Laser Harbin Institute of Technology Research Center of Laser Fusion China Academy of Engineering Physics

A 100-J-level Nd:glass laser system in nanosecond-scale pulse width has been constructed to perform as a standard source of high-fluence-laser science experiments. The laser system, operating with typical pulse durations of 3–5 ns and beam diameter 60 mm, employs a sequence of successive rod amplifiers to achieve 100-J-level energy at 1053 nm at3 ns. The frequency conversion can provide energy of 50-J level at 351 nm. In addition to the high stability of the energy output, the most valuable of the laser system is the high spatiotemporal beam quality of the output, which contains the uniform square pulse waveform, the uniform flat-top spatial fluence distribution and the uniform flat-top wavefront.

关键词： design frequency conversion laser amplifiers laser engineering laser systems light propagation modeling nonlinear optics optimization wavefront correction

Determination of molecular energies via variational-based quantum imaginary time evolution in a superconducting qubit system

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 1-11页

作者： Zhiwen Zong Sainan Huai Tianqi Cai Wenyan Jin Ze Zhan zhenxing Zhang Kunliang Bu Liyang Sui Ying Fei Yicong zheng Shengyu Zhang Jianlan Wu Yi Yin Zhejiang Province Key Laboratory of Quantum Technology and Device School of PhysicsZhejiang UniversityHangzhou 310027China Tencent Quantum Laboratory Shenzhen 518057China

As a valid tool for solving ground state problems,imaginary time evolution(ITE)is widely used in physical and chemical *** ITE-based algorithms in their quantum counterpart have recently been proposed and applied to some real *** experimentally realize the variational-based quantum imaginary time evolution(QITE)algorithm to simulate the ground state energy of hydrogen(H_2)and lithium hydride(Li H)molecules in a superconducting qubit *** H_2 molecule is directly simulated using the 3-qubit circuit with unitary-coupled clusters(UCC)*** also combine QITE with the cluster mean-field(CMF)method to obtain an effective *** Li H molecule is correspondingly simulated using the 3-qubit circuit with hardware-efficient *** comparison,the Li H molecule is also directly simulated using the 4-qubit circuit with UCC ansatz at the equilibrium *** the experimental results show a convergence within 4 iterations,with high-fidelity ground state energy *** a more complex system in the future,the CMF may allow further grouping of interactions to obtain an effective Hamiltonian,then the hybrid QITE algorithm can possibly simulate a relatively large-scale system with fewer qubits.

关键词： quantum computation quantum algorithm superconducting qubit

Biocompatibility of FGL Peptide Self-assembly Nanofibers with Neural Stem Cells in vitro

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Journal of Wuhan University of Technology(Materials Science) 2009年第6期24卷 992-996页

作者：张振兴郑启新 Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells （NSCs）, FGL pepitide-amphiphile （FGL-PA） was synthesized by solid-phase peptide synthesis technique. The diluted hydrochloric acid was added into FGL-PA solution to reduce the PH value and accordingly induce self-assembly. The morphological features of the assembled material were studied by transmission electron microscope. NSCs were cultured and added with self-assembled FGL-PA. CCK-8 kit was used to test its effect on the proliferation of NSCs. The differentiation of NSCs was also tested after FGL-PA assembled material added. The experimental results showed that FGL-PA could be self-assembled to form a hydrogel. TEM analysis showed the self-assembled hydrogel was nanofibers with diameter of 10-20 nm and length of hundreds nanometers. FGL-PA with concentrations of 50,100, or 200 mg/L could promote the proliferation of NSCs, and absorbance of them was increased （P〈0.05）. The rate of neurons differentiated from NSCs was improved greatly by FGL-PA assembled material compared with control （P〈0.05）. The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel, which had good biocompatibility with NSCs.

关键词： peptide nanofiber biomaterial neural stem cell biocompatibility

挤压铸造低体积分数SiC_w/Al复合材料的组织与性能

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特种铸造及有色合金 2017年第6期37卷 635-637页

作者：郑振兴朱德智广东技术师范学院机电学院华南理工大学机械与汽车工程学院

采用挤压铸造工艺制备了SiC_w含量为14%的Al基复合材料,并利用金相显微镜、扫描电镜(SEM)、电子万能试验机等手段分析了退火及T6处理工艺对SiC_w/Al复合材料组织和性能的影响。结果表明,SiC_w/Al复合材料中SiC_w分布均匀,无孔洞、气孔... 详细信息

采用挤压铸造工艺制备了SiC_w含量为14%的Al基复合材料,并利用金相显微镜、扫描电镜(SEM)、电子万能试验机等手段分析了退火及T6处理工艺对SiC_w/Al复合材料组织和性能的影响。结果表明,SiC_w/Al复合材料中SiC_w分布均匀,无孔洞、气孔等铸造缺陷。热处理可以显著提高材料的抗拉强度,退火和T6处理后SiC_w/Al复合材料的抗拉强度分别为321.4 MPa和392.7 MPa,相较铸态分别提高了52.9%和86.8%。结果表明,挤压铸造工艺可以制备出高强度的低体积分数铝基复合材料。

关键词：金属基复合材料挤压铸造 T6处理