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Site-specific transfer of chromosomal segments and genes in wheat engineered chromosomes

Journal of Genetics and Genomics 2017年第11期44卷 531-539页

作者： Jing Yuan Qinghua Shi Xiang Guo Yalin Liu Handong Su Xianrui Guo zhenling lv Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Recently, engineered minichromosomes have been produced using a telomere-mediated truncation technique in some plants. However, the study on transferring genes to minichromosomes is very ***, telomere-mediated truncation was successfully performed in common wheat（Triticum aestivum）to generate stable truncated chromosomes accompanied by a relatively high frequency of chromosomal rearrangements. After the cross between transgenic parents, a promoter-less DsRed gene in a chromosome from one parent was transferred to another chromosome from the other parent at the site behind a maize ubiquitin promoter via the Cre/lox system. DsRed transcripts and red fluorescent proteins were detected in the recombinant plants. In one such seedling, transgenic signals were detected at the centric terminus of chromosome 4D and the distal terminus of chromosome 3A. Clear translocations could be detected at the transgenic loci of these two chromosomes. Intriguingly, signals of centric-specific sequences were co-localized with the translocated D-group chromosomal segment in the terminal region of chromosome 3A. Our results indicate that the Cre/lox system induces the gene swapping to the target chromosome and non-homologous chromosomal recombination simultaneously. These approaches could offer a platform to transfer large DNA fragments or even terminal chromosomal segments to other chromosomes of the natural genome.

关键词： Telomere-mediated truncation Engineered chromosome Crellox system Chromosomal recombination Chromosome translocation Triticum aestivum

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Alteration of Terminal Heterochromatin and Chromosome Rearrangements in Derivatives of Wheat-Rye Hybrids

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Journal of Genetics and Genomics 2013年第8期40卷 413-420页

作者： Shulan Fu zhenling lv Xiang Guo Xiangqi Zhang Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Geneticsand Developmental BiologyChinese Academy of Sciences Key Laboratory of Plant Breeding and Genetics Sichuan Agriculture University

Wheat-rye addition and substitution lines and their self progenies revealed variations in telomeric heterochromatin and centromeres, Furthermore, a mitotically unstable dicentric chromosome and stable multicentric chromosomes were observed in the progeny of a Chinese Spring-lmperial rye 3R addition line. An unstable multicentric chromosome was found in the progeny of a 6R/6D substitution line. Drastic variation of terminal heterochromatin including movement and disappearance of terminal heterochromatin occurred in the progeny of wheat- rye addition line 3R, and the 5RS ditelosomic addition line. Highly stable minichromosomes were observed in the progeny ofa monosomic 4R addition line, a ditelosomic 5RS addition line and a 6R/6D substitution line. Minichromosomes, with and without the FISH signals for telomeric DNA （TTTAGGG）n, derived from a monosomic 4R addition line are stable and transmissible to the next generation. The results indicated that centromeres and terminal heterochromatin can be profoundly altered in wheat-rye hybrid derivatives.

关键词： Wheat-Rye addition lines Chromosome rearrangements Multiple centromeres Minichromosomes Heterochromatin

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Molecular Cytogenetic Characterization of Wheat-Thinopyrum elongatum Addition,Substitution and Translocation Lines with a Novel Source of Resistance to Wheat Fusarium Head Blight

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Journal of Genetics and Genomics 2012年第2期39卷 103-110页

作者： Shulan Fu zhenling lv Bao Qi Xiang Guo Jun Li Bao Liu Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics&Cytology Northeast Normal UniversityChangchun 130024China

Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.

关键词： Thinopyrum elongatum Alien addition lines Substitution lines Fusarium Head Blight resistance (FHB) Genomic in situ hybridization (GISH)

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Molecular Cytogenetic Characterization and Stem Rust Resistance of Five Wheat-Thinopyrum ponticum Partial Amphiploids

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Journal of Genetics and Genomics 2014年第11期41卷 591-599页

作者： Qi Zheng zhenling lv Zhixia Niu Bin Li Hongwei Li Steven S.Xu Fangpu Han Zhensheng Li State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences United States Department of Agriculture Agricultural Research ServiceCereal Crops Research Unit

Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids （Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631） using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK （commonly known as Ug99）. Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.

关键词： Thinopyrum ponticum Triticum aestivum Partial amphiploid Multicolor-genomic in situ hybridization (mc-GISH) Multicolor-fluorescent insitu hybridization (me-FISH) Stem rust

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Characterization of CENH3 proteins in diploid and allopolyploid wheat species

Characterization of CENH3 proteins in diploid and allopolypl...

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第十三届全国植物基因组学大会

作者： Jing Yuan,zhenling lv,Xiang Guo,Fangpu Han~* Institute of Genetics and Developmental Biology,Cas,Beijing 100101,China

Bread wheat(*** L.) is an important crop plants that have a complicated evolutional *** lots of studies have focused on the domestication process of wheat,little is known about the underlying molecular mechanisms of its evolution and allopolyploid *** of CENH3,a centromere-specific histone H3 variant,is reported to result in failure of centromere formation and chromosome

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The evolution of repeat sequences in wheat polyploids

The evolution of repeat sequences in wheat polyploids

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第十三届全国植物基因组学大会

作者： Xiang Guo,Shunlan Fu,zhenling lv,Fangpu Han~*,Bao Liu~* Institute of Genetics and Developmental Biology,Beijing 100101,China

Allopolyploidization,a major force in plant evolution,can bring strong "genetic shock" to the plant genome which results in great epigenetic changes and sequence *** nonrandom sequence eliminations occur not only in l...

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Integrated Communication System for Urban Emergency Command

Integrated Communication System for Urban Emergency Command

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2011 2nd Asia-Pacific Conference on Wearable Computing Systems (APWCS 2011);2011 International Conference on Intelligent Control and Information Technology(ICICIT 2011)

作者： Jing lv zhenling Liu General Office of Tianjin Municipal Government Information Technology Service Center China Electronics Technology Group Corporation No.54 Research Institute

Urban emergency communication system is an important part of the command system。In dealing with major public emergencies,we need professional,multi-arms work together,how to achieve synergy of combined arms operations in the communication command scheduling is one of the problems the urban emergency communication system should *** this paper,we integrated the urban emergency command communications dispatch system implementation project as a preliminary discussion on technical issues and propose a practical solution.

关键词： City Emergency General Communications network System

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川牛膝炮制前后阿魏酸含量比较

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光明中医 2010年第11期25卷 1992-1994页

作者：李松武王昭张振凌吕鹏河南省食品药品监督管理局郑州450053 河南中医学院郑州450008

目的对酒制前后川牛膝中阿魏酸含量进行比较,研究炮制对川牛膝中阿魏酸含量的影响。方法采用酒炙法对川牛膝进行炮制,HPLC法测定川牛膝中阿魏酸的含量。结果 HPLC法测定阿魏酸在2.368μg～7.104μg范围内线性关系良好,回归方程为:y=1×1... 详细信息

目的对酒制前后川牛膝中阿魏酸含量进行比较,研究炮制对川牛膝中阿魏酸含量的影响。方法采用酒炙法对川牛膝进行炮制,HPLC法测定川牛膝中阿魏酸的含量。结果 HPLC法测定阿魏酸在2.368μg～7.104μg范围内线性关系良好,回归方程为:y=1×10-6x-0.1658,r2=0.9999。回收率为98.53%,RSD=1.00%(n=5)。生品中阿魏酸含量在0.59～0.73之间,制品中阿魏酸含量在0.62～0.85之间。结论酒制后川牛膝中阿魏酸含量略微升高。

关键词：川牛膝酒炙阿魏酸 HPLC

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线性α-烯烃供需状况及生产工艺研究进展

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宁夏工程技术 2022年第2期21卷 133-137页

作者：程嵩鹏安良成李镇伶刘素丽吕俊敏范素兵赵天生宁夏大学化学化工学院宁夏银川750021 宁夏大学省部共建煤炭高效利用与绿色化工国家重点实验室宁夏银川750021 国家能源集团宁夏煤业集团有限责任公司宁夏银川750411

线性α-烯烃(LAO)是非常重要的有机化工原料和中间体,应用范围广泛,近年来在国内外的需求量逐年增高,其生产工艺是行业内关注的重点。介绍了LAO近年来的供需状况,并对国内外α-烯烃的主要生产工艺进行了综述,阐述了不同工艺的流程、特... 详细信息

线性α-烯烃(LAO)是非常重要的有机化工原料和中间体,应用范围广泛,近年来在国内外的需求量逐年增高,其生产工艺是行业内关注的重点。介绍了LAO近年来的供需状况,并对国内外α-烯烃的主要生产工艺进行了综述,阐述了不同工艺的流程、特点及进展。结合我国资源特点,提出了“以大力投入乙烯齐聚工艺为主、以费托蜡裂解和生物质能源路线为保障”的LAO生产工艺发展方向。

关键词：线性α-烯烃供需状况乙烯齐聚蜡裂解费托合成高碳醇脱水生物质

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慢性低氧对肺动脉高压大鼠肺血管ERK 1/2、p38MAPK表达的影响

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国际呼吸杂志 2016年第23期36卷 1804-1808页

作者：程振玲施熠炜杜永成山西省人民医院感染科太原030012 山西医科大学第一医院呼吸科太原030001 山西省人民医院呼吸科太原030012

目的：通过建立慢性低氧性肺动脉高压大鼠模型,研究慢性低氧对大鼠肺血管细胞外信号调节蛋白激酶（ERK1/2）、p38MAPK蛋白表达的影响。方法建立慢性常压低氧肺动脉高压大鼠模型,将雄性SD大鼠随机分为正常对照组、低氧1d、3d、7d、14d和... 详细信息

目的：通过建立慢性低氧性肺动脉高压大鼠模型,研究慢性低氧对大鼠肺血管细胞外信号调节蛋白激酶（ERK1/2）、p38MAPK蛋白表达的影响。方法建立慢性常压低氧肺动脉高压大鼠模型,将雄性SD大鼠随机分为正常对照组、低氧1d、3d、7d、14d和21d组,应用免疫组织化学技术检测肺动脉高压形成过程中大鼠肺血管 ERK1/2、p38MAPK 蛋白表达水平。结果①RVSP 和 RV/（lv＋S）比值较正常对照组明显增加（P〈0.05）,低氧后3 d、7 d、14 d和21 d后大鼠肺血管明显增厚；②ERK1/2、p38MAPK蛋白广泛分布于肺血管内皮细胞、平滑肌细胞和成纤维细胞中,且随着低氧时间的延长,ERK1/2、p38MAPK蛋白表达量增加。结论 ERK1/2、p38MAPK 蛋白表达量的上调可能参与了慢性低氧诱导的大鼠肺动脉高压肺血管重塑的发生、发展过程。

关键词：细胞外信号调节蛋白激酶 p38丝裂原活化蛋白激酶慢性低氧肺动脉高压肺血管重塑

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