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WTV2.0:A high-coverage plant volatilomics method with a comprehensive selective ion monitoring acquisition mode

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Molecular Plant 2024年第6期17卷 972-985页

作者： Honglun Yuan Yiding Jiangfang zhenhuan liu Rongxiu Su Qiao Li Chuanying Fang Sishu Huang Xianqing liu Alisdair R.Fernie Jie Luo School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication)and College of Tropical Agriculture and Forestry Hainan UniversitySanya Hainan 572025China Yazhouwan National Laboratory(YNL) Sanya Hainan 572025China Department of Molecular Physiology Max-Planck-Institute of Molecular Plant PhysiologyAm Mühlenberg 1Potsdam-Golm 14476Germany

Volatilomics is essential for understanding the biological functions and fragrance contributions of plant ***,the annotation coverage achieved using current untargeted and widely targeted volatomics(WTV)methods has been limited by low sensitivity and/or low acquisition ***,we introduce WTV 2.0,which enabled the construction of a high-coverage library containing 2111 plant volatiles,and report the development of a comprehensive selective ion monitoring(cSIM)acquisition method,including the selection of characteristic qualitative ions with the minimal ion number for each compound and an optimized segmentation method,that can acquire the smallest but sufficient number of ions for most plant volatiles,as well as the automatic qualitative and semi-quantitative analysis of cSIM ***,the library and acquisition method we developed can be self-expanded by incorporating compounds not present in the library,utilizing the obtained cSIM *** showed that WTV 2.0 increases the median signal-to-noise ratio by 7.6-fold compared with the untargeted method,doubled the annotation coverage compared with the untargeted and WTV 1.0 methods in tomato fruit,and led to the discovery of menthofuran as a novel flavor compound in passion *** 2.0 is a Python library with a user-friendly interface and is applicable to profiling of volatiles and primary metabolites in any species.

关键词： volatilomics widely targeted methods GC-MS selective ion monitoring plant volatiles

Research on the Curriculum System Construction Plan for Animation Design and Production of Specialty in High Vocational Colleges

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International Journal of Technology Management 2016年第1期 23-25页

作者： zhenhuan liu Department of Computer Engineering Dongguan Polytechnic Dongguan Guangdong 523808 China

In this paper, we conduct research on curriculum system construction plan for animation design and production of specialty in high vocational colleges. With the development of animation industry in our country, the existing higher vocational anime education already cannot satisfy the needs of the enterprises. Anime professional teaching reform is imminent. This article stands on the basis of existing animation specialty teaching reform, the analysis summarizes common problems, and proposes the corresponding solution hope to make their efforts to the development of animation education. We insist on teachers in the teaching process, and in the case of the classroom into more comprehensive knowledge, to improve the students＇ technology at the same time, more to improve students＇ comprehensive qualities to look for the better education performance.

关键词： Curriculum System Reform Animation Design High Vocational Colleges Specialty.

Nanoscale all-optical logic devices

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Science China(Physics,Mechanics & Astronomy) 2019年第4期62卷 14-28页

作者： Ye Chen YinKe Cheng RongBin Zhu FeiFan Wang HaoTian Cheng zhenhuan liu ChongXiao Fan YuXuan Xue ZhongCheng Yu JianKun Zhu XiaoYong Hu QiHuang Gong State Key Laboratory for Mesoscopic Physics & Department of Physics Collaborative Innovation Center of Quantum Matter Peking University Collaborative Innovation Center of Extreme Optics Shanxi University

Commercial computers based on electronic logic devices have brought great changes to the world. However, traditional electronic devices are suffering from numerous technical challenges in their attempts to continue to satisfy Moore's law. Alloptical logic devices, as promising successors to their electronic counterparts, have become a major focus of optics research. In this paper, we provide a review of current all-optical logic devices. The logic gates in these devices, which are described in the first part of the review, are divided into five categories based on the different principles used in their realization. Complex optical devices with various functions and reconfigurable devices are summarized in the next section. In the final part of this paper, we discuss some of the previous works on all-optical integrated chips with specific functions. This review will provide a complete technological roadmap for all-optical devices and aims to be helpful in possible future developments in this growing field.

关键词： optical logic elements integrated optics optical switching

Children with Autism on Music Therapy Clinical Study

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Children with Autism on Music Therapy Clinical Study

Development of a widely targeted volatilomics method for profiling volatilomes in plants

第二十三次全国儿科中西医结合学术会议

作者： zhenhuan liu Nanhai Maternity and Children’s Hospital Affiliated to Guangzhou University of Chinese Medicine

Objective: To study the intervention effect of music therapy in the treatment of children’s autism and observe the clinical effect of the therapy in improving the attention, social communication, and sensibility and emotion of the children with autism. Methods: 30 cases of children with autism from 2008 to 2009, come to outpatient of Nanhai Obstetrics and Pediatric Hospital Affiliated to Guangzhou University of Chinese Medicine, who answered for American DSMIV autism diagnosis were chosen. There are 26 male cases and 4 female cases in this group. 8 cases aged from 3 to 7 years old, 22 cases aged from 1 to 3 years old. 10 cases had cerebral anoxia history during neonatal perinatal. 20 cases had no reasons. Patients were treated with music therapy, by way of passive listening, active participation, improvisation and relaxation suggestion, 6 times a week, 30～35 min each time, running for three weeks and then resting for 10 d,60 times as a course of *** the attention, social communication, emotion and sensibility of children with autism before, process and after music therapy. The behavioral development,musical development and emotional development of the 30 cases of children with autism before, process and after music therapy were graded and analyzed with the method of paired-samples T-tests. Results: The grades of the behavioral development,musical development and emotional development of children with autism after music therapy treatment were improved comparing to those before the *** grades in the course of treatment were improved comparing to those before. The grades after treatment were improved comparing to those in the course of treatment. There were no adverse reactions in the course of treatment. The grades of the behavioral development before treatment was 18.80±5.21,in the course of treatment was 21.13±5.45, after the treatment was 25.33±5.64;The grades of the musical development before treatment was 8.87±3.18,in the course of treatm

关键词： children autism music therapy clinical study

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Molecular Plant 2022年第1期15卷 189-202页

作者： Honglun Yuan Guangping Cao Xiaodong Hou Menglan Huang Pengmeng Du Tingting Tan Youjin Zhang Haihong Zhou Xianqing liu Ling liu Yiding Jiangfang Yufei Li zhenhuan liu Chuanying Fang Liqing Zhao Alisdair R.Fernie Jie Luo College of Tropical Crops Hainan UniversityHaikou 570288China Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya572025China College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Department of Molecular Physiology Max-Planck-lnstitute of Molecular Plant PhysiologyAm Muhlenberg 114476 Potsdam-GolmGermany

Volatile organic compounds play essential roles in plant environment interactions as well as determining the fragrance of *** gas chromatography-mass spectrometry-based untargeted metabolo-mics is commonly used to assess plant volatiles,it suffers from high spectral convolution,low detection sensitivity,a limited number of annotated metabolites,and relatively poor ***,we report a widely targeted volatilomics(WTV)method that involves using a“targeted spectra extraction”algorithm to address spectral convolution,constructing a high-coverage MS2 spectral tag library to expand volatile annotation,adapting a multiple reaction monitoring mode to improve sensitivity,and using regression models to adjust for signal *** newly developed method was used to profile the volatilome of rice *** with the untargeted method,the newly developed WTV method shows higher sensitivity(for example,the signal-to-noise ratio of guaicol increased from 4.1 to 18.8),high annotation coverage(the number of annotated volatiles increased from 43 to 132),and better reproducibility(the number of volatiles in quality control samples with relative standard deviation value below 30.0%increased from 14 to 92 after normalization).Using the WTV method,we studied the metabolic responses of tomato to environmental stimuli and profiled the volatilomes of different rice *** results identified benzothiazole as a potential airborne signal priming tomato plants for enhanced defense and 2-nonanone and 2-heptanone as novel aromatic compounds contributing to rice *** case studies suggest that the widely targeted volatilomics method is more efficient than those currently used and may considerably promote plant volatilomics studies.

关键词： widely targeted volatilomics GC-MS MS2T library plant volatilome rice tomato

Effect of Interactive Scalp Acupuncture Music and therapy in Treating Autism Spectrum Disorder

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Effect of Interactive Scalp Acupuncture Music and therapy in...

Change analysis of rice area and production in China during the past three decades

第二十三次全国儿科中西医结合学术会议

作者： liu zhenhuan Jin Bingxu Zhao Yong Nanhai Affiliated Maternity and Children’s Hospital of Guangzhou Traditional Chinese Medicine University

Objective: To investigate the therapeutic effect of interactive scalp acupuncture on autism spectrum disorder. Methods: The 83 cases of children with ASD according to the visiting sequence were randomly divided into observation group and control group, the control group using music and education therapy, the observation group using interactive scalp acupuncture therapy, before treatment and after treatment were CARS, ABC and Gesell developmental quotient evaluation, continuous treatment for 3 courses after evaluation of clinical efficacy of bed. Results: The two groups of children with CARS, ABC, social adaptation DQ and personal social DQ score were statistically significant(P<0.01), and the comparison between groups was statistically significant(P<0.05).Conclusion: Scalp acupuncture therapy and music and education therapy combination can improve the social and cognitive function in patients with ASD, interactive scalp acupuncture treatment is better than single music education therapy.

关键词： Autism spectrum disorder Interactive scalp acupuncture Music and education therapy

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Journal of Geographical Sciences 2013年第6期23卷 1005-1018页

作者： liu zhenhuan LI Zhengguo TANG Pengqin LI Zhipeng WU Wenbin YANG Peng YOU Liangzhi TANG Huajun Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences / Key Laboratory of Agri-informatics Ministry ofAgriculture Beijing 100081 China Environment and Production Technology Division International Food Policy Research Institute 2033KStreet NW Washington DC 20006 USA

Rice＇s spatial-temporal distributions, which are critical for agricultural, environ- mental and food security research, are affected by natural conditions as well as socio-eco- nomic developments. Based on multi-source data, an effective model named the Spatial Production Allocation Model （SPAM） which integrates arable land distribution, administrative unit statistics of crop data, agricultural irrigation data and crop suitability data, was used to get a series of spatial distributions of rice area and production with 10-km pixels at a national scale -it was applied from the early 1980s onwards and used to analyze the pattern of spatial and temporal changes. The results show that significant changes occurred in rice in China during 1980-2010. Overall, more than 50% of the rice area decreased, while nearly 70% of rice production increased in the change region during 1980-2010. Spatially, most of the increased area and production were in Northeast China, especially, in Jilin and Heilongjiang; most of the decreased area and production were located in Southeast China, especially, in regions of rapidly urbanization in Guangdong, Fujian and Zhejiang. Thus, the centroid of rice area was moved northeast approximately 230 km since 1980, and rice production about 320 km, which means rice production moved northeastward faster than rice area because of the significant rice yield increase in Northeast China. The results also show that rice area change had a decisive impact on rice production change. About 54.5% of the increase in rice pro- duction is due to the expansion of sown area, while around 83.2% of the decrease in rice production is due to contraction of rice area. This implies that rice production increase may be due to area expansion and other non-area factors, but reduced rice production could largely be attributed to rice area decrease.

关键词： rice distribution spatial production allocation model spatial-temporal changes China

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发育性运动协调障碍儿童的早期筛查与干预

中国儿童保健杂志 2024年第5期32卷 475-479页

作者：刘振寰广州中医药大学附属南海妇产儿童医院广东佛山528200

发育性协调障碍(DCD)是一种神经发育障碍性疾病,发病率高,诊断率低,症状出现早,阻碍儿童身心健康发展。DCD的早期识别与干预有利于减轻该疾病的终身不良影响。但目前国内大众对该病仍缺乏认识,医疗工作者对DCD的早期筛查及干预不够重视... 详细信息

发育性协调障碍(DCD)是一种神经发育障碍性疾病,发病率高,诊断率低,症状出现早,阻碍儿童身心健康发展。DCD的早期识别与干预有利于减轻该疾病的终身不良影响。但目前国内大众对该病仍缺乏认识,医疗工作者对DCD的早期筛查及干预不够重视。本文概述发育性协调障碍的临床特征、早期筛查与诊断,并从西医干预、中医干预两个层面阐述DCD的临床治疗,首次提出中西医结合干预治疗,希望以此促进DCD儿童早期筛查工作的开展,为DCD的干预在我国的研究和发展提供新的方法,提高其康复效果。

关键词：发育性协调障碍早期筛查中西医结合干预

Spatio-temporal dynamics of maize cropping system in Northeast China between 1980 and 2010 by using spatial production allocation model

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Journal of Geographical Sciences 2014年第3期24卷 397-410页

作者： TAN Jieyang YANG Peng liu zhenhuan WU Wenbin ZHANG Li LI Zhipeng YOU Liangzhi TANG Huajun LI Zhengguo Key Laboratory of Agri-informatics Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 China Geography and Planning School of Sun Yat-sen University Guangzhou 510275 China Environment and Production Technology Division International Food Policy Research Institute 2033K Street NW Washington DC 20006 USA

Understanding crop patterns and their changes on regional scale is a critical re- quirement for projecting agro-ecosystem dynamics. However, tools and methods for mapping the distribution of crop area and yield are still lacking. Based on the cross-entropy theory, a spatial production allocation model （SPAM） has been developed for presenting spa- tio-temporal dynamics of maize cropping system in Northeast China during 1980-2010. The simulated results indicated that （1） maize sown area expanded northwards to 48~N before 2000, after that the increased sown area mainly occurred in the central and southern parts of Northeast China. Meanwhile, maize also expanded eastwards to 127°E and lower elevation （less than 100 m） as well as higher elevation （mainly distributed between 200 m and 350 m）; （2） maize yield has been greatly promoted for most planted area of Northeast China, espe- cially in the planted zone between 42°N and 48°N, while the yield increase was relatively homogeneous without obvious longitudinal variations for whole region; （3） maize planting density increased gradually to a moderately high level over the investigated period, which reflected the trend of aggregation of maize cultivation driven by market demand.

关键词： spring maize spatial production allocation model spatio-temporal pattern Northeast China