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The Crop Journal 2023年第1期11卷 46-56页

作者： Na Wang Jun Chen Yuan Gao Yongbin Zhou Ming Chen Zhaoshi Xu Zhengwu Fang Youzhi Ma Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture Yangtze UniversityJingzhou 434000HubeiChina Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementKey Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureBeijing 100081China

Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome.The chromosomal locations and structures of the genes,protein properties,and phylogenetic relationships were characterized.ATP/ADP TaIPT genes showed tissue-specific expression.TaIPT2,TaIPT7,and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments,which decreased to low levels after 2 h drought treatment,as did most other TaIPT genes.TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance,which,however,did not manifest as phenotype alterations.By contrast,transgenic wheat plants with drought-induced TaIPT8showed increased drought tolerance.Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.

关键词： TaIPT Phytohormone Abiotic stress Cytokinin biosynthesis Wheat

Genome-wide Analysis of a Plant AT-rich Sequence and Zinc-binding Protein (PLATZ) in Triticum Aestivum

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 971-986页

作者： Xiaohang He Minjie Liu Zhengwu Fang Dongfang Ma Yilin Zhou Junliang Yin Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of EducationHubei Collaborative Innovation Center for Grain IndustryCollege of AgricultureYangtze UniversityJingzhou434025China Shanxi Key Laboratory of Integrated Pest Management in Agriculture Institute of Plant ProtectionTaiyuan030031China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193China

Plant AT-rich sequence and zinc-binding protein(PLATZ)is a plant transcription factor that has been studied in corn.PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression.It is involved in the regulation of dehydration tolerance in seeds.In this study,we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes.We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice,Arabidopsis,and maize.Phylogenetic analysis on PLATZ gene sequences from different species was performed.We found that PLATZs could be divided into three groups.The chromosome(chr)distribution analysis revealed that the 49 identified wheat PLATZ genes are distributed in 15 chrs.Gene structure and motif analyses indicated that most PLATZ genes possess conserved exon/intron arrangements and motif compositions.Our analysis of transcriptional data indicated that several wheat PLATZ genes may play an important role in abiotic stress resistance given that they are expressed under salt stress.The results of qRT-PCR further confirmed that TaPLATZ is involved in plant abiotic stress and is also related to the cell differentiation of plant tissues.Our results lay the foundation for further studies on the function of the wheat PLATZ gene family.

关键词： Transcription factor PLATZ abiotic stress gene expression

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中华民族现代文明建设的文化逻辑与实践路向

思想理论教育导刊 2023年第12期 44-52页

作者：詹小美房正武中山大学马克思主义学院

社会主义文化强国与中华民族现代文明建设,彰显了中华民族以中国式现代化铸就现代文明新辉煌的理性认知和行为自觉。在现实性上,文化与文明的共生发展和双向律动,以历史必然性的时代拓展构成了二者链接的内生逻辑;中华文明历史延续性、... 详细信息

社会主义文化强国与中华民族现代文明建设,彰显了中华民族以中国式现代化铸就现代文明新辉煌的理性认知和行为自觉。在现实性上,文化与文明的共生发展和双向律动,以历史必然性的时代拓展构成了二者链接的内生逻辑;中华文明历史延续性、全体人民共同富裕、“五个文明”协调发展、走和平发展道路的现实向度,表征了中华民族现代文明建设的整体性、普惠性、协调性和包容性特质;推进文化自信自强、秉持开放包容、坚持守正创新、践行社会主义核心价值观、构建中国话语和中国叙事体系,则以精神底气的涵育、发展坐标的明晰、价值根基的夯实、时代新人的培养和中华文明传播力影响力的增强,构成了中华民族现代文明建设的实现策略。

关键词：中华民族现代文明文化逻辑现实向度实践路向

Identification of stable quantitative trait loci underlying waterlogging tolerance post-anthesis in common wheat(Triticum aestivum)

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The Crop Journal 2023年第4期11卷 1163-1170页

作者： Fugong Ding Jingyang Tong Rui Xu Jing Chen Xiaoting Xu Muhammad Nadeem Shuping Wang Yingxin Zhang Zhanwang Zhu Fengju Wang Zhengwu Fang Yuanfeng Hao MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Co-construction by Ministry and Province) College of AgricultureYangtze UniversityJingzhou 434025HubeiChina National Wheat Improvement Centre Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Queensland Alliance for Agriculture and Food Innovation The University of QueenslandSt LuciaQLD 4072Australia Food Crops Institute Hubei Academy of Agricultural SciencesWuhan 430064HubeiChina

Waterlogging is a growing threat to wheat production in high-rainfall areas.In this study,a doubled haploid(DH) population developed from a cross between Yangmai 16(waterlogging-tolerant) and Zhongmai895(waterlogging-sensitive) was used to map quantitative trait loci(QTL) for waterlogging tolerance using a high-density 660K single-nucleotide polymorphism(SNP) array.Two experimental designs,waterlogging concrete tank(CT) and waterlogging plastic tank(PT),were used to simulate waterlogging during anthesis in five environments across three growing seasons.Waterlogging significantly decreased thousand-kernel weight(TKW) relative to non-waterlogged controls,although the degree varied across lines.Three QTL for waterlogging tolerance were identified on chromosomes 4AL,5AS,and 7DL in at least two environments.All favorable alleles were contributed by the waterlogging-tolerant parent Yangmai16.QWTC.caas-4AL exhibited pleiotropic effects on both enhancing waterlogging tolerance and decreasing plant height.Six high-confidence genes were annotated within the QTL interval.The combined effects of QWTC.caas-4AL and QWTC.caas-5AS greatly improved waterlogging tolerance,while the combined effects of all three identified QTL(QWTC.caas-4AL,QWTC.caas-5AS,and QWTC.caas-7DL) exhibited the most significant effect on waterlogging tolerance.Breeder-friendly kompetitive allele-specific PCR(KASP) markers(K_AX_111523809,K_AX_108971224,and K_AX_110553316) flanking the interval of QWTC.caas-4AL,QWTC.caas-5AS,and QWTC.caas-7DL were produced.These markers were tested in a collection of 240 wheat accessions,and three superior polymorphisms of the markers distributed over 67elite cultivars in the test population,from the Chinese provinces of Jiangsu,Anhui,and Hubei.The three KASP markers could be used for marker-assisted selection(MAS) to improve waterlogging tolerance in wheat.

关键词： Genetic mapping QTL Waterlogging tolerance Wheat

甜荞花同源异型基因FeMADS1的克隆和序列结构分析

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西北农业学报 2015年第3期24卷 82-87页

作者：方正武刘志雄长江大学农学院作物遗传育种研究所湖北荆州434025 长江大学园艺园林学院湖北荆州434025

采用同源克隆方法,并结合RACE技术,从甜荞花芽分离得到1个A类MADS-box基因FeMADS1的cDNA全长,GenBank登录号为KM386627,其cDNA全长1 107bp,包括1个编码234个氨基酸、长为705bp的开放阅读框。序列同源比对和分子系统发生分析表明,其蛋白... 详细信息

采用同源克隆方法,并结合RACE技术,从甜荞花芽分离得到1个A类MADS-box基因FeMADS1的cDNA全长,GenBank登录号为KM386627,其cDNA全长1 107bp,包括1个编码234个氨基酸、长为705bp的开放阅读框。序列同源比对和分子系统发生分析表明,其蛋白与拟南芥AGL8(FUL)的相似性最高,属A类MADS-box基因亚家族中的euFUL进化系,含MADS、I、K和C末端4个明显的结构域,并且K结构域包含K1、K2和K3共3个保守的富含疏水氨基酸残基的亚结构域,C末端结构域含FUL型基因2个特有的模体:FUL motif和paleoAP1motfi。

关键词：甜荞 MADS-box基因 FeMADS1 花发育

Inhibitory Effects of Phenamacril, Tebuconazole and Their Mixtures on Fusarium graminearum in Wheat

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Plant Diseases and Pests 2018年第3期9卷 14-17页

作者： Lei Cuiyu Yin Junliang fang zhengwu Wang Shuping Zhang Xing Ma Dongfang Engineering Research Center of Ecology and Agricultural Use of Wetland the Ministry of Education/College of Agriculture Yangtze University Institute of Plant Protection Jiangsu Academy of Agricultural Sciences

Fusarium head blight caused by Fusarium graminearum is one of the most serious diseases in wheat production worldwide, which seriously affects the yield and quality of wheat. Wheat grains carrying the pathogen are poisonous to human and livestock health. With the long-term use of single chemical pesticide, the drug-resistance of F. graminearum is increasing day by day, and mixed agent is one of the effective strategies to improve the efficacy and delay the resistance. The mycelial growth rate method was used to determine the inhibition rates of phenamacril, tebuconazole and their mixtures(1∶1, 1∶3, 1∶5, 1∶7, 3∶1, 5∶1 and 7∶1). The synergistic coefficient(SR) was calculated by Wadley method, and the combined action types of the two agents were evaluated. The results showed that the EC_(50) of tebuconazole was 0.418 28 mg/L, and that of phenamacril was 0.489 8 mg/L; the optimum mixture ratio of phenamacril and tebuconazole was 1 ∶1, with the EC_(50) value of 0.053 74 and the synergistic coefficient of 4.2696, and the control effect of mixed agent was significantly better than that of single agent. It is feasible to control Fusarium head blight by mixing phenamacril and tebuconazole, providing a theoretical basis for development of new compound agents and effective control of Fusarium head blight.

关键词： Phenamacril Tebuconazole Fusarium head blight Resistance

粮食技术扩散中家庭农场采纳意愿——基于河南省家庭农场调查

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江苏农业科学 2019年第4期47卷 326-333页

作者：房正武陈思河南农业大学经济与管理学院河南郑州450000

选取河南省滑县、辉县、济源市、浚县等4个县(市)的50家土地经营规模在33.33 hm^2以上的家庭农场(均为县级及以上示范性家庭农场)作为实证研究主体,以优质小麦种植技术采纳为研究案例,试图找到采纳家庭农场粮食技术意愿的影响因素和采... 详细信息

选取河南省滑县、辉县、济源市、浚县等4个县(市)的50家土地经营规模在33.33 hm^2以上的家庭农场(均为县级及以上示范性家庭农场)作为实证研究主体,以优质小麦种植技术采纳为研究案例,试图找到采纳家庭农场粮食技术意愿的影响因素和采纳家庭农场粮食技术的最优路径。通过对上述地区实地走访、问卷调查、收集数据等,建立河南省家庭农场粮食技术采纳意愿模型,分析感知有用性、感知易用性、家庭农场特征、技术特征和外部环境因素对家庭农场采纳意愿的影响路径,运用偏最小二乘路径分析法,利用Smart PLS 3.0软件对所收集调研数据进行整理和分析,得到家庭农场粮食技术采纳意愿所受影响因素的路径系数,从而找到适合家庭农场最优的粮食技术扩散路径,提出政策建议。

关键词：家庭农场技术采纳模型采纳意愿偏最小二乘法路径系数政策建议

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肺原发性类癌CT影像分析

实用医学影像杂志 2021年第5期22卷 491-493页

作者：方政雄康正武福建省漳州市医院影像科 363000

目的分析肺原发性类癌的CT影像学特征,提高对该病的诊断准确率。方法收集经病理证实肺原发性类癌19例,回顾性分析其临床及CT表现。其中17例完成CT增强扫描,2例仅CT平扫。结果所有病例均为单发,13例表现为边界光滑的类圆形孤立结节或肿块... 详细信息

目的分析肺原发性类癌的CT影像学特征,提高对该病的诊断准确率。方法收集经病理证实肺原发性类癌19例,回顾性分析其临床及CT表现。其中17例完成CT增强扫描,2例仅CT平扫。结果所有病例均为单发,13例表现为边界光滑的类圆形孤立结节或肿块影,3例为长条状沿血管支气管束走行,17例边界光滑圆润;10例密度均匀,4例内部可见钙化,3例见小囊状低密度影;2例表现为腔内结节-“冰山征”,伴阻塞性炎症7例,4例出现同侧及纵隔淋巴结肿大,2例累及邻近胸膜。CT增强扫描:15例出现明显强化,CT值增幅超过40 HU。仅2例呈轻度强化。本组19例均无空洞影及明显胸腔积液,2例于1年后出现远处转移征象。结论肺类癌CT表现缺乏特征性;肺内及支气管腔边界光滑的结节或肿块,增强扫描明显较均匀明显强化,“冰山征”等可提高本病的诊断准确率。

关键词：肺类癌螺旋CT影像学诊断

Inhibition Effect and Synergistic Effect of Prochloraz and Tebuconazole and Their Mixture on Fusarium graminearum

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Plant Diseases and Pests 2017年第6期8卷 28-30页

作者： Zhang Xing Zhang Lin He Yiqin Lu Chen fang zhengwu Zhao Mingmin Zhu Yongxing Ma Dongfang Hubei Collaborative Innovation Center of Main Grain Crop Industrialization/Collage of Agriculture Yangtze University Hubei Provincial Bureau of Agricultural Reclamation Management

Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is growing day by day. In order to reveal the inhibition effect and synergistic effect of prochloraz and tebuconazole and their mixture on F. graminearum,new compound agents for controlling wheat scab were developed. The inhibition effect and synergistic effect of prochloraz and tebuconazole mixed at seven different ratios on F. graminearum were studied. The results showed that the efficiency of the mixture was the best when the proportion of prochloraz and tebuconazole was 1:7; the EC50 was 0.145 36 and the synergistic coefficient was 1.853 9. The results provide a theoretical basis for development of new compound agents and effective control of wheat scab.

关键词： Fusarium graminearum Prochloraz Tebuconazole Compound agent Sensitivity

Sensitivity of Fusarium graminearum to Carbendazim and Prochloraz

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Plant Diseases and Pests 2017年第1期8卷 21-23页

作者： Ma Dongfang Huang Shi Zhang Peng Sun Cai Huang Wendi Li Guangjun Zhang Changqing fang zhengwu Hubei Collaborative Innocvtion Center for Grain Industry/Yangtze University Jingzhou 434025 China

[ Objective] The paper was to understand the resistance of Fusarium graminearum to carbendazim and prochloraz in the main wheat producing areas in Hubei Province. [ Method] Totally 350 F. graminearum strains isolated in 2014 were determined to clear their sensitivity to carbendazim and prochloraz. [ Result] ALl isolates were sensitive to carbendazim and procidoraz in Hubei Province, and carbendazim was still valuable in controlling Fusarium head blight. The control effect of prochloraz was better than that of carbendazim, and the combination of prochloraz and carbendazim at the proportion of 1：7 received the best control effect. [ Conclusion ] Combination of chemical agents will occupy a more and more important position in future prevention and control against Fusarium head blight.

关键词： Fusarium graminearum Carbendazim Prochloraz Sensitivity Fungicide resistance