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Microeukaryotic plankton community dynamics under ecological water replenishment:Insights from eDNA metabarcoding

Environmental Science and Ecotechnology 2024年第4期20卷 278-290页

作者： Shuping Wang Songsong Gu Yaqun Zhang Ye Deng Wenhui Qiu Qianhang Sun Tianxu Zhang Pengyuan Wang zhenguang yan State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing100012China CAS Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing100085China Key Laboratory of Aquatic Genomics Ministry of Agriculture and Rural AffairsBeijing Key Laboratory of Fishery BiotechnologyChinese Academy of Fishery SciencesBeijing100141China College of Resources and Environment University of Chinese Academy of SciencesBeijing100190China Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen518055China

Ecological water replenishment(EWR)is an important strategy for river restoration globally,but timely evaluation of its ecological effects at a large spatiotemporal scale to further adjust the EWR schemes is of great ***,we examine the impact of EWR on microeukaryotic plankton communities in three distinct river ecosystems through environmental DNA(eDNA)*** three ecosystems include a long-term cut-off river,a short-term connected river after EWR,and long-term connected *** analyzed community stability by investigating species composition,stochastic and deterministic dynamics interplay,and ecological network *** found that EWR markedly reduced the diversity and complexity of microeukaryotic plankton,altered their community dynamics,and lessened the variation within the ***,EWR disrupted the deterministic patterns of community organization,favoring dispersal constraints,and aligning with trends observed in naturally connected *** shift from an isolated to a temporarily connected river appeared to transition community structuring mechanisms from deterministic to stochastic dominance,whereas,in permanently connected rivers,both forces concurrently influenced community *** ecological network in temporarily connected rivers post-EWR demonstrated significantly greater stability and intricacy compared to other river *** shift markedly bolstered the resilience of the ecological *** eDNA metabarcoding insights offer a novel understanding of ecosystem resilience under EWR interventions,which could be critical in assessing the effects of river restoration projects throughout their life cycle.

关键词： Ecological water replenishment Community stability Succession Community assembly mechanism Ecological network

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基于环境DNA的长江中华鲟分布特征探究

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环境工程技术学报 2024年第1期14卷 71-78页

作者：周权杜浩王洁邵芸闫振广环境基准与风险评估国家重点实验室中国环境科学研究院中国水产科学研究院长江水产研究所

中华鲟(Acipenser sinensis)是我国长江流域的旗舰物种,在长江十年禁渔的大背景下,研究中华鲟无损化检测技术具有重要意义。环境DNA(eDNA)技术是一种环境友好型生物监测技术,可以在不直接观察或捕获生物体的情况下对物种进行检测。从文... 详细信息

中华鲟(Acipenser sinensis)是我国长江流域的旗舰物种,在长江十年禁渔的大背景下,研究中华鲟无损化检测技术具有重要意义。环境DNA(eDNA)技术是一种环境友好型生物监测技术,可以在不直接观察或捕获生物体的情况下对物种进行检测。从文献中筛选出可以用于检测中华鲟eDNA的特异性引物,于2020年9月在长江中下游选取4个中华鲟常出现的区域,进行各断面立体式采样;提取16个点位的e DNA,使用筛选得到的引物对中华鲟进行eDNA的检测,以探究中华鲟的分布特征。结果显示,成功筛选到1组可以检测中华鲟eDNA的引物,使用该引物成功检测到包括中华鲟在内的长江4种鲟类的eDNA,共计测得约300万条鲟类序列。依据测序结果分析不同断面检测到的中华鲟eDNA的差异,发现宜昌江段断面的中华鲟eDNA最多,洞庭湖口断面最少,且表层和底层水体的中华鲟eDNA检出也有显著差异。筛选得到的引物可以用于中华鲟eDNA的检测,中华鲟e DNA的检测结果与中华鲟的历史调查和洄游特征较为吻合。不同水深条件中华鲟eDNA的检出量有显著差异,表明在今后的调查中采用混合或者立体采样可以更加全面地进行中华鲟eDNA的检测。

关键词：中华鲟特异性引物环境DNA 操作分类单元长江

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杭州湾溶解性无机氮、活性磷酸盐营养物基准研究

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环境科学研究 2024年第7期37卷 1412-1422页

作者：魏超凌佳楠闫振广安立会邓邦平欧梦圆郑欣中国环境科学研究院环境基准与风险评估国家重点实验室北京100012 南方海洋科学与工程广东省实验室广州珠海528315 自然资源部东海生态中心自然资源部海洋生态监测与修复技术重点实验室上海201206 浙江省生态环境科学设计研究院国家环境保护水污染控制工程技术(浙江)中心浙江杭州310007

制定氮磷营养物基准是预防和控制水体富营养化的重要手段。本研究梳理了2010-2022年杭州湾历史监测数据,通过主成分分析、相关性分析等方法筛选了杭州湾营养物基准关键指标,并综合运用频数分布法、压力-响应模型等方法对指标进行统计分... 详细信息

制定氮磷营养物基准是预防和控制水体富营养化的重要手段。本研究梳理了2010-2022年杭州湾历史监测数据,通过主成分分析、相关性分析等方法筛选了杭州湾营养物基准关键指标,并综合运用频数分布法、压力-响应模型等方法对指标进行统计分析,推导营养物基准值。结果表明:①溶解性无机氮(DIN)和活性磷酸盐(SPR)是制定杭州湾营养物基准的关键营养物指标,其浓度变化直接影响水体的富营养化程度和生态系统的稳定性;Chla和DO则是关键生物学指标,可用于评价水体健康程度。②由于频数分布法和压力-响应模型法的适用性不同,两种方法推导出的杭州湾营养物基准值存在较大差异,由压力响应模型法得出的营养物基准值比频数分布法高。③根据杭州湾目前的水质状况和富营养化水平,本研究提出杭州湾DIN、SPR的基准值分别为0.732和0.033 mg/L。研究显示,采用多种方法联合推导出的营养物基准值更符合水生态保护的实际需求,本研究结果可为杭州湾水体富营养化控制及营养物标准修订提供科学支撑。

关键词：杭州湾富营养化营养物基准频数分布法压力-响应模型

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Preliminary analysis of species sensitivity distribution based on gene expression effect

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Science China Earth Sciences 2012年第6期55卷 907-913页

作者： yan zhenguang yanG NiYun WANG XiaoNan WANG WanHua MENG ShuangShuang LIU ZhengTao State Key Laboratory of Environmental Criteria and Risk Assessment State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals Chinese Research Academy of Environmental Sciences Beijing 100012 China

Species sensitivity analysis is one of the major techniques applied to derive water quality criteria. Presently, the toxicity data used for development of water quality criteria are mainly in the biological individual level. With the increase of ecotoxicogenomics toxicity data, it is worth studying whether the gene expression effect data can be used to derive water quality criteria. Taking cadmium, copper and zinc as examples, we analyzed the toxic effects of the three heavy metals by constructing the species sensitivity distribution curves on the basis of extensive toxicity data. The results showed that the rank of species sensitivity for the acute, chronic and gene expression effect toxicity data of cadmium is "chronic>gene>acute". Although the gene expression effect data of copper and zinc are insufficient, the trend of data sensitivity of zinc is similar to cadmium. However, the trend of species sensitivity of copper is different from that of cadmium and zinc with higher sensitivity of gene expression data. It suggested that though the existing data of gene expression effects are not sufficient enough, they have the potential to be used in the development of chronic water quality criteria. For application in the derivation of water quality criteria, illogical test concentration design and insufficient target genes are two main weaknesses in the study of gene expression effects.

关键词： water quality criteria heavy metal gene expression effect species sensitivity distribution data analysis

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A possible mechanism for the formation of annual growth lines in bivalve shells

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Science China(Life Sciences) 2011年第2期54卷 175-180页

作者： LIU XiaoJun yan zhenguang ZHENG GuiLan ZHANG GuiYou WANG HongZhong XIE LiPing ZHANG RongQing Institute of Marine Biotechnology School of Life Sciences Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China Key Lab of Ecological Effect and Risk Assessment for Chemicals Chinese Research Academy of Environmental Sciences Beijing 100012 China

We report a unique shell margin that differed from the usual shell structure of Pinctada *** observed empty organic envelopes in the prismatic layer and the formation of the nacreous layer in the shell *** the characteristics of the growing margin indicated that the shell was growing *** explain this anomaly,we propose the concept of "jumping development".During jumping development,the center of growth in the bivalve shell jumps forward over a short time interval when the position of the mantle *** development explains the unusual structure of the anomalous shell and the development of annual growth lines in typical *** growth lines are the result of a discontinuity in the shell microstructure induced by jumping development.

关键词： bivalves shell microstructure internal growth bands the prismatic layer the nacreous layer

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High specificity detection of Pb^2＋ ions by p-SCN-Bz-DTPA immunogen and p-NH2-Bn-DTPA coating antigen

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Frontiers of Environmental Science & Engineering 2014年第5期8卷 729-736页

作者： Ruozhen YU Zheng FANG Wei MENG zhenguang yan Lina DU Hong WANG Zhengtao LIU State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences Beijing 100012 China

Only one bifunctional metal-chelator was used to prepare immunogen and coating antigen in all of the previous researches. However, the antibody-specific recognition to the spacer arm of the bifunctional metal- chelator might lower the specificity of heavy metal ions immunoassay. Two different bifunctional metal-chelators were adopted to prepare the immunogen and coating antigen respectively in our study to avoid this problem. The conjugates of keyhole limpet hemocyanin （KLH） and p-SCN-Bz-DTPA-Pb were used as immunogen, whereas the conjugates of bovine sentrn albumin （BSA） and p- NH2-Bn-DTPA-Pb were used as coating antigen. Poly- clonal antibodies specific to DTPA-Pb chelates were obtained from rabbits. Indirect competitive enzyme-linked immunosorbent assay （ELISA） was adopted to detect Pb^2＋ ion solutions prepared by Pb^2＋ standard solution and ultrapure water. In the mixing microplate, DTPA and Pb2＋ ions formed chelates and combined with specific anti- bodies. After incubation, the DTPA-Pb and the antibodies complex were added into the wells of the reaction microplate. The reaction microplate was coated by the conjugates ofBSA andp-NH2-DTPA-Pb, which competed for the specific antibodies. The result signals presented a good sigmoid curve when the Pb^2＋ concentration ranges from 0.01 to 100mg·L^-1 The IC50 of the indirect competitive ELISA is 0.23±0.04mg·L^-1 Pb2＋ ion. The cross-reaction with Cd^2＋, Cu^2＋, Fe^2＋, Mn^2＋, Zn^2＋, and other divalent ions were less than 5%.

关键词： p-SCN-Bz-DTPA p-NH2-Bn-DTPA leadion polyclonal antibody

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Study on the four Qi of Pfaffia glomerata based on the metabolomics technology and comparison of Dangshen(Radix Codonopsis) in the equivalent substitution prescription

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Journal of Traditional Chinese Medicine 2024年第4期44卷 713-721页

作者： SONG zhenguang yanG Bin WANG Fei yan Dongmei ZHOU Xiaoqing HUANG Liping GAO Xuemei LI Bin HUANG Luqi Academician Workstation Jiangxi University of Chinese MedicineNanchang 330004China State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China School of PharmacyChengdu University of Traditional Chinese MedicineChengdu 610075China National Resource Center for Chinese Materia Medica Chinese Academy of Chinese Medical Sciences(CACMS)Beijing 100700China

OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese ***:The normal group,six cold herb groups,six hot herb groups,six cool herb groups,PG prescription group(PGPG),and Dangshen(Radix Codonopsis)prescription group(CPPG)were prepared with corresponding concentrations of water extracts,these herb extracts were administered by gavage to SpragueDawley rats,and the 12 h urine at night on the 29th day of the SD rats in each group were collected,Liquid Chromatograph Mass Spectrometer system was used to analyze them,the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up,so as to the medicinal properties of PGPG,CPPG and PG were *** on the Progenesis QI,Human Metabolome Database,Kyoto Encyclopedia of Genes and Genomes,MetaboAnalyst 5.0 database,we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG ***:We established a best model of cold-hot herbal discrimination in the positive ion mode,then the probability that PGPG was predicted cold property was 88.9%.Furthermore,a model of cold-cool herbal discrimination was established,then the probability of PGPG containing the cool property was 77.8%.In addition,typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline,(R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in *** regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine ***:The results showed PGPG had a cool medicinal property as same as CPPG,and the regulation trend of PGPG on markers was consistent with cool ***,the medicine properties PG and CP should be consistent,and the Traditional Chinese Medicine p

关键词： Pfaffia glomerata medicinal properties of Chinese medicine metabolomics molecular mechanism equivalent substitution

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In vivo and in vitro biomineralization in the presence of the inner-shell film of pearl oyster

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Acta Oceanologica Sinica 2011年第1期30卷 87-93页

作者： yan zhenguang MENG Wei LIU Zhengtao yanG Suwen LIU Xiaojun SUN Juan XIE Liping ZHANG Rongqing Institute of Marine Biotechnology Department of Biological Sciences and BiotechnologyTsinghua University Beijing 100084 China Chinese Research Academy of Environmental Sciences Beijing 100012 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing100084 China

The inner shell surface is the biomineralization site in shell formation and an inner-shell film covers it. This surface is composed of two regions： an outer calcitic region and an inner aragonitic region. In this study, some amalgamated calcite crystals were found in the calcitic region and some aragonitic ＂imprints＂ were found in the central part of the aragonitic region. The ＂imprints＂ are probably the trace of mantle cells that adhered to the inner shell surface when the shell was produced. Furthermore, to build a novel in vitro biomineralization system, the inner-shell film was detached from the shell and introduced to the calcitic crystallization solution. Crystallization experiments showed that nacre proteins could induce aragonite crystals in the novel system but inhibited calcite growth in the absence of the inner-shell film. These data suggested that the inner-shell film may induce aragonite growth in vivo by combining nacre proteins.

关键词： biomineralization inner shell surface inner-shell film nacre Pinctada fucata

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Development of emergency water quality standard for Cr^(6+) and Hg^(2+) in Liao River basin

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Engineering Sciences 2014年第2期12卷 45-51页

作者： yan zhenguang Zheng Xin Ye Jing Wang Xiaonan Wu Jiangyue Liu Zhengtao Meng Wei State Key Laboratory of Environmental Criteria and Risk Assessment State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of ChemicalChinese Research Academy of Environmental Sciences Chemical Registration Centre of Ministry of Evironmental Protection of China

The acute toxicity data of Cr＋ and Hg2＋ to the aquatic organisms in Liao River basin was collected and screened. Calculated results of criteria values using popular species sensitivity distribution （SSD） methods were evaluated through data analysis. The comprehensive methodology of emergency water quality standards （EWQSs） was established on the basis of the SSD method of Australia and New Zealand according to the evalu- ation results. The affected fractions of the aquatic organisms were set to be 5 %, 15 %, 30 % and 50 %, corre- sponding to the risk grades of Ⅰ,Ⅱ,Ⅲ and IV, and four-grade EWQSs, respectively. The EWQSs for Cr6＋ and Hg2. in the Liao River were derived and the corresponding risk indicators were also proposed. The results showed that the four-grade EWQSs for Cr6＋ were 2.85 μg/L, 21.0 μg/L, 161 μg/L and 797 μ/L, respec- tively, and the risk indicators were daphnia （grade Ⅱ ）, shrimp （grade Ⅲ）, and the bighead fish or tadpole （grade IV）. The four-grade EWQSs for Hg2＋ were 0.59 μg/L, 2.32 μg/L, 6.25 μg/L and 15.6 μg/L, respective- ly, and the risk indicators were crucian carp or daphnia （grade Ⅱ）, shrimp （grade Ⅲ）, and worm or crab （grade IV）. The results provided valuable information for the risk analysis of sudden ambient water environmen- tal pollution accident.

关键词： Liao River Cr6+ Hg2+ emergency water quality standards risk indicators

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Localization of calmodulin and calmodulin-like protein and their functions in biomineralization in P. fucata

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Progress in Natural Science:Materials International 2008年第4期18卷 405-412页

作者： Zi Fang a, zhenguang yan a, Shuo Li a, Qin Wang a, Weizhong Cao a, Guangrui Xu a, Xunhao Xiong a, Liping Xie a,b, Rongqing Zhang a,b, a Institute of Marine Biotechnology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China b Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China Institute of Marine Biotechnology Department of Biological Science and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

Calmodulin (CaM) and calmodulin-like protein (CaLP) are two proteins involved in biomineralization. Their localizations in Pinct- ada fucata mantle epithelia were studied by Western blot (WB) analysis of the nuclear/cytosol fraction of primary cultured P. fucata mantle cells and immunogold electron microscopy. The results showed a completely different distribution of these two proteins at the subcellular level. CaM was distributed throughout both the nucleus and cytoplasm of the mantle epithelium but CaLP was distributed only in the cytoplasm. The functions of these two proteins in biomineralization were investigated by shell regeneration. During this pro- cess, the expressions of CaM and CaLP were greatly enhanced in different organelles of the mantle epithelium. Overexpression of these two proteins and a mutant of calmodulin-like protein (M-CaLP) that lacks an extra C-terminal tail in MC3T3-E1 promoted the mRNA expression of osteopontin, a biomineralization marker for osteoblasts. All of the results indicated that CaM and CaLP have completely different distributions in the mantle epithelium and affect the biomineralization process at different levels. The extra C-terminal tail of CaLP is important for its functions in biomineralization in P. fucata.

关键词： Pinctada fucata Calmodulin Calmodulin-like protein Immunogold electron microscopy

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