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Pm21, Encoding a Typical CC-NBS-LRR Protein, Confers Broad-Spectrum Resistance to Wheat Powdery Mildew Disease

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Molecular Plant 2018年第6期11卷 879-882页

作者： Huagang He Shanying Zhu Renhui Zhao zhengning jiang Yaoyong Ji Jian Ji Dan Qiu Hongjie Li Tongde Bie School of Food and Biological Engineering Jiangsu University Zhenjiang Jiangsu 212013 China School of Environment Jiangsu University Zhenjiang Jiangsu 2120131 China Key Laboratory of Wheat Biology and Genetic Improvement on Low & Middle Yangtze River Valley Wheat Region (Ministry of Agriculture) Yangzhou Academy of Agricultural Sciences Yangzhou Jiangsu 225007 China National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Dear Editor, Common wheat （Triticum aestivum, 2n = 6x = 42, AABBDD） is one of the most widely cultivated cereal crops, providing -20% of the calories consumed by humans （Fu et al., 2009）. However, wheat production is constantly challenged by powdery mildew disease, which occurs globally and is caused by the biotrophic fungal pathogen Blumena graminis f. sp. tritici （Bgt）. Utilization of powdery mildew resistance （Pm） genes is the most effective and economical way to control powdery mildew disease.

关键词： Pm21 疾病蛋白质小麦粉编码用途普通小麦小麦生产

The gene Pm21,encoding a typical CC-NBS-LRR protein,confers broad spectrum resistance to wheat powdery mildew

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The gene Pm21,encoding a typical CC-NBS-LRR protein,confers ...

A genome-wide association study of Fusarium head blight resistance in common wheat(Triticum aestivum L.)

第八届全国小麦基因组学及分子育种大会

作者： Huagang He Shanying Zhu Yaoyong Ji zhengning jiang Renhui Zhao Tongde Bie jiangsu University Yangzhou Academy of Agricultural Sciences

Common wheat is the most widely grown cereal crop providing 0% of the calories consumed by ***,wheat production is challenged constantly by devastating powdery mildew caused by Blumeria graminis ***(Bgt).Utilization of powdery mildew resistance(Pm) genes is an effective and economical way to reduce yield losses caused by *** to now,more than 100 Pm genes in wheat and its wild relatives have been documented,among which,the Pm21 gene,originating from Dasypyrum villosum,confers high resistance to all known Bgt *** is important to clarify the genetic basis and functional mechanism of *** this study,fine genetic mapping of Pm21 was conducted on an F population(harboring 10,536 individuals)derived from the cross between the resistant *** line DvRes-1 and the susceptible line DvSus-1,and a high-density map was ***21 was mapped to a 0.01-cM interval flanked by the markers 6 VS-08.4 b and 6 VS-10 *** on comparative mapping,a conserved CC-NBS-LRR-encoding resistance gene analog(RGA) locus was found between wheat and *** to the conserved RGA locus,a large genomic sequence harboring three complete genes DvPP2 C,encoding a protein phosphatase,DvRGA2 and DvRGA1,as well as a separated gene DvRGA3,were then obtained by PCR *** analysis showed that all these genes co-segregate *** analysis demonstrated both DvRGA1 and DvRGA2 responded to Bgt invasion while DvRGA3 had no ***,the silencing effects of DvRGA1 and DvRGA2 in Yangmai 18 carrying Pm21 were analyzed by using the BSMV-VIGS *** silencing of DvRGA2 could cause the normal devebpment of Bgt spores and macroscopic disease symptom on ***,no obvious effects were observed after silencing of *** 6,408 M families of Yangmai 18 treated with EMS,a total of 58 independent lines highly susceptible to powdery mildew during the whole growth stage were screened *** for Y18-S16

关键词： Dasypyrum villosum wheat powdery mildew resistance DvRGA2 Pm21

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A genome-wide association study of Fusarium head blight resi...

小麦DNA去甲基化酶基因全基因组鉴定及其在籽粒发育中的表达分析

第十届全国小麦基因组学及分子育种大会

作者： Wenjing Hu Derong Gao Hongya Wu Jian Liu Chunmei Zhang Junchan Wang zhengning jiang Yeyu Liu Dongsheng Li Yong Zhang Chenbin Lu Institute of Agriculture Sciences of the Lixiahe District Branch of National Wheat Improvement Center

We performed a genome-wide association study(GWAS) using the high-density wheat single nucleotide polymorphism(SNP) assays to better understand the genetic basis of FHB resistance and identify associated molecular *** resistance to FHB fungal spread along the rachis(Type Ⅱ resistance) was evaluated on a nature wheat population in two growing *** Illumina Infinum iSelect 90 K SNP genotyping data,genome-wide association study(GWAS) identified new loci for Type II resistance,which were found on syntenic genomic regions of chromsome 4 A and chromosome 5 D,showing highly collinarity in gene content and *** FHB resistant cultivars and associated SNP markers identified in this study are valuable for FHB resistance breeding via marker-assisted selection.

关键词： Triticum aestivum L. Fusarium head blight(FHB) Mixed linear model(MLM) Genome-wide association study(GWAS) Single nucleotide polymorphism(SNP)

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麦类作物学报 2023年第11期43卷 1361-1371页

作者：蒋正宁刘大同张晓江伟王玲李东升程晓明高德荣江苏里下河地区农业科学研究所/农业部长江中下游小麦生物与遗传改良重点实验室江苏扬州225007

DNA去甲基化酶(dMTase)是一种高度保守的表观遗传修饰因子,涉及许多生物学过程,包括生长发育、应激反应和次生代谢。本研究基于小麦基因组数据,对小麦DNA去甲基化酶基因(TadMTase)进行了全面鉴定和生物信息学分析。结果表明,小麦基因组... 详细信息

DNA去甲基化酶(dMTase)是一种高度保守的表观遗传修饰因子,涉及许多生物学过程,包括生长发育、应激反应和次生代谢。本研究基于小麦基因组数据,对小麦DNA去甲基化酶基因(TadMTase)进行了全面鉴定和生物信息学分析。结果表明,小麦基因组中包含18个TadMTase基因,分布于小麦15条染色体上。系统进化分析将TadMTase分为ROS、DML3、DML4和DML5等4个亚家族,亚家族之间的TadMTase基因序列长度和内含子数量存在差异,但同一个系统进化树分支中的亚族成员具有高度相似的基因结构、保守motifs和结构域,为植物dMTase基因家族的直系同源基因,在进化方面具有保守性。亚细胞定位预测TadMTase均定位于细胞核中;通过与小麦祖先物种的进化及共线性分析,发现在小麦异源六倍体形成过程中存在部分TadMTase基因丢失;TadMTase基因家族启动子区域包含大量光信号、植物激素、胁迫响应和生长发育等相关顺式作用元件;转录组数据分析表明TadMTase基因在不同组织器官和籽粒发育不同时期表达模式不同,有一定的组织特异性。进一步RNA-Seq和荧光定量PCR分析表明TaROS1b-1A.1和TaROS1a-5A/D分别在籽粒的种皮和胚乳发育时期显著上调表达,且在强筋和弱筋小麦品种中表达存在差异。结果为TadMTase基因在调控小麦籽粒生长发育及其品质形成中的调控机制提供参考。

关键词：小麦(Triticum aestivum) DNA去甲基化酶基因家族籽粒表达分析

小麦木瓜类半胱氨酸蛋白酶基因TaRD21的克隆及赤霉病菌诱导下的表达分析

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麦类作物学报 2023年第7期43卷 819-826页

作者：蒋正宁高致富寿露露江伟王玲陈甜甜张勇高德荣江苏里下河地区农业科学研究所/农业部长江中下游小麦生物与遗传改良重点实验室江苏扬州225007

木瓜类半胱氨酸蛋白酶(PLCPs)作为一类重要的蛋白水解酶,在植物生长发育以及胁迫应答过程中都发挥着重要作用。本研究从抗、感赤霉病小麦品种差异表达基因谱中获得1个注释为RD21 Cysteine proteases的EST(表达序列标签),以此序列检索小... 详细信息

木瓜类半胱氨酸蛋白酶(PLCPs)作为一类重要的蛋白水解酶,在植物生长发育以及胁迫应答过程中都发挥着重要作用。本研究从抗、感赤霉病小麦品种差异表达基因谱中获得1个注释为RD21 Cysteine proteases的EST(表达序列标签),以此序列检索小麦最新基因组数据库并设计引物,从小麦中克隆到3个基因,分别命名为TaRD21-2A、TaRD21-2B和TaRD21-2D,属于PLCPs RD21家族。序列分析表明,3个基因的开放阅读框长度分别为1410、1428和1419 bp,分别编码469、475和472个氨基酸。序列比对发现,3个基因的序列相似性为89.3%,所编码蛋白的氨基酸序列相似性为95.6%。系统进化分析表明,TaRD21-2A、TaRD21-2B和TaRD21-2D蛋白的同源性较高,且与乌拉尔图小麦TuRD21A蛋白聚为一类。qRT-PCR分析表明,3个TaRD21基因均受水杨酸(SA)、乙烯利(ETH)以及赤霉病菌诱导表达;感病品种中,TaRD21-2A对SA和赤霉病菌的响应更迅速,且表达量较高;抗病品种中,TaRD21-2B和TaRD21-2D基因对ETH的响应更迅速。

关键词：小麦木瓜类半胱氨酸蛋白酶 TaRD21 赤霉病菌表达分析

小麦不同灌浆速率品种籽粒淀粉合成与积累研究

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植物生理学报 2021年第3期57卷 703-712页

作者：刘大同蒋正宁张晓刘健杨阳周宾寒朱冬梅高德荣江苏里下河地区农业科学研究所农业农村部长江中下游小麦生物学与遗传育种重点实验室江苏扬州225007 扬州大学/江苏省粮食作物现代产业技术协同创新中心江苏扬州225009 长江大学农学院湖北荆州434023

为探明小麦(Triticum aestivum)不同灌浆速率品种籽粒淀粉合成与积累差异的生理基础,本研究以长江中下游麦区灌浆速率较快的品种‘扬麦16’和‘扬麦23’为材料,以灌浆速率较慢的‘扬麦15’和‘宁麦13’为对照,测定不同灌浆时间籽粒中淀... 详细信息

为探明小麦(Triticum aestivum)不同灌浆速率品种籽粒淀粉合成与积累差异的生理基础,本研究以长江中下游麦区灌浆速率较快的品种‘扬麦16’和‘扬麦23’为材料,以灌浆速率较慢的‘扬麦15’和‘宁麦13’为对照,测定不同灌浆时间籽粒中淀粉含量,通过树脂半薄切片和扫描电镜观察胚乳淀粉体发育特征,采用荧光定量PCR测定灌浆期籽粒淀粉合成酶基因相对表达量,并测定淀粉合成关键酶活性。结果表明:‘扬麦16’和‘扬麦23’灌浆盛期单粒淀粉合成量大于对照,形态学表现为胚乳细胞内淀粉体大,充实快,且B型淀粉体形成早,淀粉体总体发育进程快。灌浆前中期,‘扬麦16’和‘扬麦23’中AGPaseI、GBSSI、SSSIII、SBEI等4种基因表达量总体高于对照,并且ADP-葡萄糖焦磷酸化酶(AGPase)、可溶性淀粉合成酶(SSS)、结合态淀粉合成酶(GBSS)和淀粉分支酶(SBE)活性也均高于‘扬麦15’和‘宁麦13’。本研究表明,‘扬麦16’和‘扬麦23’灌浆期淀粉体较大、淀粉合成关键酶活性高是淀粉合成与积累快的主要原因。

关键词：小麦灌浆速率淀粉合成淀粉体基因表达关键酶

94份小麦种质Puroindoline和HMW-GS分子检测与品质分析

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植物遗传资源学报 2024年第4期25卷 509-521页

作者：张晓江伟高德荣郭延玲刘大同蒋正宁李曼刘健袁博陆成彬江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室扬州225007 玉米生物育种全国重点实验室沈阳110164

籽粒硬度和高分子量谷蛋白亚基(HMW-GS)对小麦品质起决定作用,为发掘和利用硬度Puroindoline基因和HMW-GS优异等位变异,提升长江中下游麦区中强筋小麦品质,对长江中下游麦区推广品种以及其他麦区优质推广品种和地方品种共计94份材料进... 详细信息

籽粒硬度和高分子量谷蛋白亚基(HMW-GS)对小麦品质起决定作用,为发掘和利用硬度Puroindoline基因和HMW-GS优异等位变异,提升长江中下游麦区中强筋小麦品质,对长江中下游麦区推广品种以及其他麦区优质推广品种和地方品种共计94份材料进行分子检测和品质分析。结果表明,硬度变幅7.21~72.91,软质类型42份、占44.68%,硬质类型42份、占44.68%,混合类型10份、占10.64%。硬度突变基因型共有5种,包括Pina-D1b/Pinb-D1a、Pina-D1r/Pinb-D1a、Pina-D1s/Pinb-D1a、Pina-D1a/Pinb-D1b和Pina-D1a/Pinb-D1p,数量分别为8份、3份、1份、29份和9份,籽粒硬度表现依次为Pina-D1r/Pinb-D1a>Pina-D1s/Pinb-D1a>Pina-D1b/Pinb-D1a>Pina-D1a/Pinb-D1p>Pina-D1a/Pinb-D1b。HMW-GS分析表明,Glu-A1位点1和Null亚基材料比例分别为53.33%和45.56%,此外有1G330E亚基材料1份;Glu-B1位点7+8和7+9亚基材料比例分别为47.78%和46.67%,此外有14+15亚基材料3份、7OE+8*亚基材料1份、6+8亚基材料1份;Glu-D1位点2+12和5+10亚基材料比例分别为61.11%和38.89%。在微量SDS沉淀值上,Glu-A1位点的1和Null亚基、Glu-B1位点的7+8和7+9亚基无显著差异,Glu-D1位点5+10亚基极显著大于2+12亚基。硬度和SDS沉淀值呈极显著正相关,硬度对SDS沉淀值影响大于HMW-GS。本研究对小麦种质Pin和HMW-GS基因型和品质进行了分析,为小麦品质遗传改良尤其是中强筋小麦品质改良提供了参考。

关键词：小麦硬度 Puroindoline HMW-GS 分子检测品质分析

Copper Hydride-Catalyzed Conjugate Reduction-Aldol Addition Domino Reaction of α,β-Unsaturated Carboxylates with Ketones

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Chinese Journal of Chemistry 2013年第4期31卷 539-544页

作者： Zengchang Li Lan jiang zhengning Li Huiying Chen College of Environmental and Chemical Engineering Dalian University Dalian Liaoning 116622 China Liaoning Key Laboratory of Bioorganic Chemistry Dalian University Dalian Liaoning 116622 China College of Life Science Dalian Nationalities University Dalian Liaoning 116600 China

Copper hydride-catalyzed conjugate reduction-intermolecular aldol addition domino reactions were realized using α,β-unsaturated carboxylates as hydride acceptors and a silane as the reducing reagent. High diastereoselec- tivities were achieved with 4,5-bis（diphenylphosphino）-9,9-dimethylxanthene as the ligand and tert-butyl acrylate as the hydride acceptor.

关键词： reduction aldol addition domino reaction copper hydride catalysis

An Efficient Synthesis of 3-Alkyl-2-alkyliminooxazolidin-4-one via a Domino Reaction

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Chinese Journal of Chemistry 2011年第2期29卷 303-308页

作者： Liu Yongwei Li zhengning jiang Lan Zheng Aijun College of Environmental and Chemical Engineering Dalian University Dalian Liaoning 116622 China Liaoning Key Laboratory of Bioorganic Chemistry Dalian University Dalian Liaoning 116622 China

3-Alkyl-2-alkyliminooxazolidin-4-ones were prepared from a copper-catalyzed domino intermolecular nucleophilic addition of a-hydroxy-（or mecapto） carboxylic acid ester to carbodiimide and a followed nucleophilic substitution of the formed amino group to carboxylic acid ester. The reactions could be performed under convenient conditions and good yields were achieved in most cases.

关键词： iminooxazolidin-4-one synthesis carbodiimide a-hydroxycarboxylate