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Ductile and ultrahigh-strength eutectic high-entropy alloys by large-volume 3D printing

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Journal of Materials Science & Technology 2022年第31期126卷 15-21页

作者： Yiping Lu Xiaoxiang wu zhenghong Fu Qiankun Yang Yong Zhang Qiming Liu Tianxin Li Yanzhong Tian Hua Tan Zhiming Li Tongmin Wang Tingju Li High Entropy Alloy Material Engineering Research Center(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian 116024China School of Iron and Steel Soochow UniversitySuzhou 215137China School of Materials Science and Engineering Central South UniversityChangsha 410083China Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China

*** Additive manufacturing or 3D printing outweighs conventional casting methods in the aspect of complex parts fabrication,which can realize one-step formation without the need of complicated cast dies.3D printing significantly promotes industrial production for making near-net shaped ***,this promising technique is not always ideally applicable for metals and *** example,titanium alloys prepared by 3D printing often suf-fer from poor plasticity,and usually require further complex heat treatment or hot isostatic pressing treatment,in order to remove internal stress and regulate plasticity and strength[1,2],which de-feats the original intention of employing additive *** of the fundamental causes for such issues is the low fluidity of the alloys upon melting,leading to great chemical heterogeneity,high porosity content and residual *** limitation hinders further design and fabrication of high-performance printable alloys from large scale production and application.

关键词： alloys strength eutectic

海南省39株幽门螺杆菌的耐药性及毒力基因特征

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贵州医科大学学报 2024年第4期49卷 479-488页

作者：卓书伟林美幸吴翔潘静琳周海清陈海陈峥宏贵州医科大学基础医学院微生物学教研室贵州贵阳550025 海南省中医院检验科海南海口570203 海南省中医院消化内科海南海口570203 海南省中医院医院感染管理科海南海口570203

目的了解海南省幽门螺杆菌(Hp)耐药性及其毒力基因vacA、cagA和iceA基因型特征。方法对146例上消化道症状疾病患者进行胃镜检查,取胃黏膜组织进行Hp的分离和培养,对Hp进行药敏检测;通过聚合酶链式反应(PCR)对毒力基因cagA、vacA和iceA... 详细信息

目的了解海南省幽门螺杆菌(Hp)耐药性及其毒力基因vacA、cagA和iceA基因型特征。方法对146例上消化道症状疾病患者进行胃镜检查,取胃黏膜组织进行Hp的分离和培养,对Hp进行药敏检测;通过聚合酶链式反应(PCR)对毒力基因cagA、vacA和iceA进行检测和分型,cagA测序进行聚类分析、并构建系统进化树;使用Fisher确切概率法分析各型毒力基因和患者疾病发生的相关性。结果共培养出39株Hp,对阿莫西林和克拉霉素的耐药率分别为2.6%和48.7%;39株均为vacA^(+)菌株,iceA 1型26株、2型13株;33株为cagA^(+)菌株,其中5株为西方型(EPIYA-ABC型),28株为东亚型(EPIYA-ABD型);cagA^(+)和vacA^(+)菌株与胃和十二指肠疾病有显著的相关性(P0.05)。结论海南省胃和十二指肠Hp患者中Hp毒力基因多样化,vacA s1am2/cagA^(+)基因型在消化性溃疡患者中占优势,不同毒力基因型对胃和十二指肠疾病的发生具有明显的差别,阿莫西林可作为该地区根除Hp首选抗生素。

关键词：幽门螺杆菌耐药性基因多样性消化性溃疡 cagA vacA iceA

Genomic insights into the origin, adaptive evolution, and herbicide resistance of Leptochloa chinensis, a devastating tetraploid weedy grass in rice fields

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Molecular Plant 2022年第6期15卷 1045-1058页

作者： Lifeng Wang Xuepeng Sun Yajun Peng Ke Chen Shan wu Yanan Guo Jingyuan Zhang Haona Yang Tao Jin Lamei wu Xiaomao Zhou Bin Liang zhenghong Zhao Ducai Liu Zhangjun Fei Lianyang Bai State Key Laboratory of Hybrid Rice Hunan Academy of Agricultural SciencesChangsha 410125China Hunan Weed Science Key Laboratory Hunan Academy of Agricultural SciencesChangsha 410125China Boyce Thompson Institute Cornell UniversityIthacaNY 14853USA College of Horticulture Science Zhejiang A&F UniversityHangzhou 311300China Qingdao Kingagroot Compounds Co.Ltd Qingdao 266000China USDA-ARS Robert W.Holley Center for Agriculture and HealthIthacaNY 14853USA

Chinese sprangletop (Leptochloa chinensis), belonging to the grass subfamily Chloridoideae, is one of the most notorious weeds in rice ecosystems. Here, we report a chromosome-scale reference genome assembly and a genomic variation map of the tetraploid L. chinensis. The L. chinensis genome is derived from two diploid progenitors that diverged ∼10.9 million years ago, and its two subgenomes display neither fractionation bias nor overall gene expression dominance. Comparative genomic analyses reveal substantial genome rearrangements in L. chinensis after its divergence from the common ancestor of Chloridoideae and, together with transcriptome profiling, demonstrate the important contribution of tetraploidization to the gene sources for the herbicide resistance of L. chinensis. Population genomic analyses of 89 accessions from China reveal that L. chinensis accessions collected from southern/southwestern provinces have substantially higher nucleotide diversity than those from the middle and lower reaches of the Yangtze River, suggesting that L. chinensis spread in China from the southern/southwestern provinces to the middle and lower reaches of the Yangtze River. During this spread, L. chinensis developed significantly increased herbicide resistance, accompanied by the selection of numerous genes involved in herbicide resistance. Taken together, our study generated valuable genomic resources for future fundamental research and agricultural management of L. chinensis, and provides significant new insights into the herbicide resistance as well as the origin and adaptive evolution of L. chinensis.

关键词： Leptochloa weed sgenome polyploidization genome evolution genetic diversity adaptive selection herbicide resistance

热纤梭菌Cthe_2401蛋白功能预测及在大肠杆菌中的克隆表达与纯化

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贵州医科大学学报 2024年第4期49卷 501-507页

作者：崔古贞管玉祝王鑫鑫花登雄吴道艳吴晓娟钟欣言洪伟陈峥宏贵州医科大学基础医学院微生物学教研室贵州贵阳550025 贵州省病原生物学特色重点实验室贵州贵阳550004 贵州省分子生物学重点实验室贵州贵阳550004

目的利用生信手段预测Cthe_2401蛋白的特性与功能,并在大肠杆菌中进行体外克隆、表达与纯化。方法利用国家生物技术信息中心数据库(NCBI)、蛋白保守结构域数据库(CDD)分析Cthe_2401蛋白的序列和进化特征,采用SWISS-MODEL在线软件进行同... 详细信息

目的利用生信手段预测Cthe_2401蛋白的特性与功能,并在大肠杆菌中进行体外克隆、表达与纯化。方法利用国家生物技术信息中心数据库(NCBI)、蛋白保守结构域数据库(CDD)分析Cthe_2401蛋白的序列和进化特征,采用SWISS-MODEL在线软件进行同源建模并预测该蛋白的结构与功能;利用聚合酶链式反应(PCR)扩增Cthe_2401基因,并与pET28a载体链接构建pET28a-Cthe_2401表达质粒,转化大肠杆菌后利用异丙基硫代半乳糖(IPTG)诱导Cthe_2401蛋白表达;利用50%硫酸铵沉淀、Ni-NTA柱以及SephadexG75葡聚糖凝胶柱纯化Cthe_2401蛋白。结果生物信息学预测表明,Cthe_2401蛋白属于Veg蛋白家族,可能与生物膜的发生和孢子形成有关;Cthe_2401蛋白成功在大肠杆菌克隆与表达,体外纯化获得约10 ku大小的蛋白条带,与预期结果一致。结论成功预测Cthe_2401蛋白的功能,成功获得Cthe_2401蛋白在大肠杆菌中的克隆、表达与纯化。

关键词：热纤梭菌 Cthe_2401 Veg蛋白生物信息学蛋白表达与纯化大肠杆菌

新型抗菌肽Mt-22S3对白念珠菌细胞的作用及机制

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贵州医科大学学报 2024年第3期49卷 375-380,396页

作者：李彩多曾晔石艳萍张迎春吴建伟陈峥宏王涛贵州医科大学基础医学院贵州贵阳550025 贵州省普通高等学校病原生物学特色重点实验室贵州贵阳550025

目的探讨家蝇抗真菌肽-1A(MAF-1A)突变体-22S3(Mt-22S3)对白念珠菌(***)细胞的作用及机制。方法取对数生长期的***菌悬液,分别与0、125、250及500 mg/L Mt-22S3作用12 h,采用荧光显微技术及荧光检测法观测Mt-22S3作用***后细胞内活性氧(... 详细信息

目的探讨家蝇抗真菌肽-1A(MAF-1A)突变体-22S3(Mt-22S3)对白念珠菌(***)细胞的作用及机制。方法取对数生长期的***菌悬液,分别与0、125、250及500 mg/L Mt-22S3作用12 h,采用荧光显微技术及荧光检测法观测Mt-22S3作用***后细胞内活性氧(ROS)的变化;以荧光检测法测定Mt-22S3作用***后细胞内线粒体膜电位水平的变化;通过Annexin V-FITC/PI双染法,观察Mt-22S3对***细胞凋亡的影响;以琼脂糖凝胶电泳阻滞实验分析Mt-22S3对***细胞DNA的影响。结果经Mt-22S3作用的***细胞内ROS增高,且随着Mt-22S3浓度升高,细胞内ROS上升(P<0.05);线粒体膜电位检测发现,经Mt-22S3作用的***线粒体膜电位下降(P<0.01);Mt-22S3可引起***细胞凋亡及细胞坏死的发生,且Mt-22S3浓度越高,细胞凋亡及细胞坏死现象越明显;凝胶阻滞实验结果显示,Mt-22S3与*** DNA孵育后产生明显的凝胶阻滞现象。结论Mt-22S3可进入***细胞内发挥抗菌作用,其机制与诱导细胞内ROS积累、去极化线粒体膜、诱导细胞凋亡和死亡、结合DNA等有关。

关键词：白念珠菌活性氧细胞凋亡抗菌肽线粒体膜电位 DNA凝胶阻滞

RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response

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Signal Transduction and Targeted Therapy 2020年第1期5卷 196-205页

作者： Zezhong Liu Wei Xu Shuai Xia Chenjian Gu Xinling Wang Qian Wang Jie Zhou Yanling wu Xia Cai Di Qu Tianlei Ying Youhua Xie Lu Lu zhenghong Yuan Shibo Jiang Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) School of Basic Medical Sciences and BSL-3 facilityFudan UniversityShanghai 200032China

The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective *** receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)*** contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 ***,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 *** mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 *** results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV.

关键词： ACE2 vaccine neutral

The zinc finger transcription factor,KLF2,protects against COVID-19 associated endothelial dysfunction

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2502-2510页

作者： Suowen Xu Yujie Liu Yu Ding Sihui Luo Xueying Zheng Xiumei wu zhenghong Liu Iqra Ilyas Suyu Chen Shuxin Han Peter J.Little Mukesh K.Jain Jianping Weng Institute of Endocrine and Metabolic Diseases The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina Department of Obstetrics and Gynecology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhuiP.R.China Department of Endocrinology and Metabolism The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina Sunshine Coast Health Institute University of the Sunshine CoastBirtinyaQLDAustralia School of Pharmacy Pharmacy Australia Centre of Excellencethe University of QueenslandWoolloongabbaQLDAustralia Department of Medicine Case Cardiovascular Research InstituteCase Western Reserve UniversityClevelandOHUSA Department of Medicine Harrington Heart and Vascular InstituteUniversity Hospitals Cleveland Medical CenterClevelandOHUSA The First Affiliated Hospital Bengbu Medical CollegeBengbuChina

Coronavirus disease 2019(COVID-19)is regarded as an endothelial disease(endothelialitis)with its patho-mechanism being incompletely *** evidence has demonstrated that endothelial dysfunction precipitates COVID-19 and its accompanying multi-organ ***,pharmacotherapies targeting endothelial dysfunction have potential to ameliorate COVID-19 and its cardiovascular *** objective of the present study is to evaluate whether kruppel-like factor 2(KLF2),a master regulator of vascular homeostasis,represents a therapeutic target for C0VID-19-induced endothelial ***,we demonstrate that the expression of KLF2 was reduced and monocyte adhesion was increased in endothelial cells treated with COVID-19 patient serum due to elevated levels of pro-adhesive molecules,ICAM1 and ***-1β and TNF-α;two cytokines elevated in cytokine release syndrome in COVID-19 patients,decreased KLF2 gene ***(atorvastatin and tannic acid)and genetic(adenoviral overexpression)approaches to augment KLF2 levels attenuated COVID-19-serum-induced increase in endothelial inflammation and monocyte ***-generation RNA-sequencing data showed that atorvastatin treatment leads to a cardiovascular protective transcriptome associated with improved endothelial function(vasodilation,antiinflammation,antioxidant status,anti-thrombosis/-coagulation,anti-fibrosis,and reduced angiogenesis).Finally,knockdown of KLF2 partially reversed the ameliorative effect of atorvastatin on COVID-19-serum-induced endothelial inflammation and monocyte ***,the present study implicates loss of KLF2 as an important molecular event in the development of COVID-19-induced vascular disease and suggests that efforts to augment KLF2 levels may be therapeutically beneficial.

关键词： KLF2 inflammation elevated

针刺干预阿尔茨海默病的功能磁共振成像研究进展

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中华中医药杂志 2024年第8期39卷 4253-4256页

作者：保琼楠银子涵李雅琴张欣悦夏嫚泽陈正红姚瑾钟婉琪吴可欣张振勇梁繁荣成都中医药大学针灸推拿学院成都610000 云南省第一人民医院中医科昆明650000

针刺是干预阿尔茨海默病(AD)的有效手段,功能磁共振成像(fMRI)技术有助于为针刺干预AD的中枢机制提供客观依据。文章综述了近10年针刺干预AD的fMRI研究文献,从研究设计、分析方法及响应脑区/网络等方面进行归纳并总结,发现静息态fMRI采... 详细信息

针刺是干预阿尔茨海默病(AD)的有效手段,功能磁共振成像(fMRI)技术有助于为针刺干预AD的中枢机制提供客观依据。文章综述了近10年针刺干预AD的fMRI研究文献,从研究设计、分析方法及响应脑区/网络等方面进行归纳并总结,发现静息态fMRI采用低频振幅和基于种子点的功能连接法,观察针刺后的脑功能变化,任务态fMRI观察针刺时出现的脑区激活。AD患者的响应脑区有扣带回、额下回、额中回、额上回、颞中回等,响应脑网络包括默认模式网络、额顶网络和视觉网络。但当前针刺干预AD的fMRI研究设计较单一,分析方法较局限,今后仍需拓展研究策略以深入探索针刺干预AD的中枢作用机制。

关键词：针刺功能磁共振成像阿尔茨海默病综述分析方法响应脑区

Stereocontrolled construction of dihydrothiopyrano[2,3-b]indole skeleton via organocatalyzed asymmetric cascade aza-Michael-aldol reaction

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Stereocontrolled construction of dihydrothiopyrano[2,3-b]ind...