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Effects of maternal methyl donor intake during pregnancy on ileum methylation and function in an intrauterine growth restriction pig model

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1173-1189页

作者： Yan Lin Jiangnan Wu Yong Zhuo Bin Feng zhengfeng fang Shengyu Xu Jian Li Hua Zhao De Wu Lun Hua Lianqiang Che Key Laboratory of Animal Disease‑Resistance Nutrition Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina

Background Intrauterine growth retardation(IUGR)affects intestinal growth,morphology,and function,which leads to poor growth performance and high *** present study explored whether maternal dietary methyl donor(MET)supplementation alleviates IUGR and enhances offspring’s growth performance by improving intestinal growth,function,and DNA methylation of the ileum in a porcine IUGR *** Forty multiparous sows were allocated to the control or MET diet groups from mating until *** farrowing,8 pairs of IUGR and normal birth weight piglets from 8 litters were selected for sampling before suckling *** The results showed that maternal MET supplementation tended to decrease the IUGR incidence and increased the average weaning weight of ***,maternal MET supplementation significantly reduced the plasma concentrations of isoleucine,cysteine,urea,and total amino acids in sows and newborn *** also increased lactase and sucrase activity in the jejunum of newborn *** addition resulted in lower ileal methionine synthase activity and increased betaine homocysteine S-methyltransferase activity in the ileum of newborn *** methylation analysis of the ileum showed that MET supplementation increased the methyla-tion level of DNA CpG sites in the ileum of newborn ***-regulated differentially methylated genes were enriched in folic acid binding,insulin receptor signaling pathway,and endothelial cell *** contrast,up-regulated methylated genes were enriched in growth hormone receptor signaling pathway and nitric oxide biosyn-thetic *** Maternal MET supplementation can reduce the incidence of IUGR and increase the weaning litter weight of piglets,which may be associated with better intestinal function and methylation status.

关键词： Ileum Intrauterine growth restriction Methyl donor Methylation Sows

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Standardized ileal digestibility of amino acids in soybean meal fed to non-pregnant and pregnant sows

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Journal of Animal Science and Biotechnology 2024年第1期15卷 362-373页

作者： Ke Wang Ya Wang Lei Guo Yong Zhuo Lun Hua Lianqiang Che Shengyu Xu Ruinan Zhang Jian Li Bin Feng zhengfeng fang Xuemei Jiang Yan Lin De Wu Institute of Animal Nutrition Key Laboratory for Animal Disease‑Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130SichuanChina

Background Two studies were designed to determine standard ileal crude protein(CP)and amino acid(AA)digestibility of soybean meal(SBM)from different origins fed to non-pregnant and pregnant *** solvent-extracted SBMs from soybeans produced in the USA,Brazil,and China were *** Exp.1,eight different diets were created:a nitrogen(N)-free diet and 7 experimental diets containing SBM from different origins as the only N *** non-pregnant,multiparous sows were arranged in an 8×8 Latin square design(8 periods and 8 diets).In Exp.2,the diet formula was the same as in *** gestating sows(parity 3)were assigned to 4 different diets in a replicated 4×3 Youden square design(three periods and four diets)in mid-gestation and again in late-gestation *** When fed to non-pregnant and late-gestating sows,the standardized ileal digestibility(SID)of CP and most AAs from different SBM were not significantly different(P>0.05).When fed to mid-gestating sows,the SID values for Arg,His,Lys,Phe,Cys,Gly,Ser,and Tyr in SBM 1 were lower than in SBM 4 and 5(P<0.05),whereas SID for Leu from SBM 5 was higher than in SBM 1 and 4(P<0.05).SID values for Ile,Ala,and Asp from SBM 4 were lower than in SBM 1 and 5(P<0.05).Sows had significantly greater SID values for Lys,Ala,and Asp during mid-gestation when compared with late-gestation stages(P<0.05).Mid-gestating sows had greater SID value for Val and lower SID value for Tyr when compared with non-pregnant and late-gestating sows(P<0.01),whereas non-pregnant sows had significantly greater SID value for Met when compared with gestating sows(P<0.01).Conclusions When fed to mid-gestating sows,the SID values for most AAs varied among SBM *** SID values for Lys,Met,Val,Ala,Asp,and Tyr in SBM were affected by sow gestation *** findings provide a cornerstone for accurate SBM use in sow diets.

关键词： Amino acids Sows Soybean meal Standardized ileal digestibility

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儿童配方奶粉对小鼠骨骼发育影响的研究

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食品与发酵工业 2024年第3期50卷 100-109页

作者：杨丽刘恬欣方正峰敖晓琳韩国全陈洪四川农业大学食品学院四川雅安625014

该研究比较2种奶粉对地塞米松诱导小鼠骨骼发育不良的调节作用。选取雌性ICR小鼠,随机分为对照组、地塞米松组、地塞米松+奶粉1组、地塞米松+奶粉2组,分别给予相应饲料饲喂8周,采用肌肉注射1 mg/kg地塞米松建立骨骼发育不良模型,每周3次... 详细信息

该研究比较2种奶粉对地塞米松诱导小鼠骨骼发育不良的调节作用。选取雌性ICR小鼠,随机分为对照组、地塞米松组、地塞米松+奶粉1组、地塞米松+奶粉2组,分别给予相应饲料饲喂8周,采用肌肉注射1 mg/kg地塞米松建立骨骼发育不良模型,每周3次,实验结束时检测基础指标、血清生化指标和肠道菌群的变化。结果表明,与地塞米松组相比,2种奶粉添加后都能改善小鼠的骨微结构。血清生化分析表明,奶粉补充后血清骨转换标志物水平上升,血清促炎细胞因子水平下降,抗炎细胞因子水平增加,血清中钙含量增加。肠道菌群分析表明,奶粉补充后能显著改变肠道菌群的组成。在门水平上,奶粉1的添加增加了Verrucomicrobiota的相对丰度,奶粉2的添加增加了Firmicutes的相对丰度,降低了Bacteroidota的相对丰度。在属水平上,奶粉1的添加能上调Akkermansia和Alloprevotella的相对丰度,而奶粉2的添加能上调Lactobacillus和Lachnoclostridium的相对丰度。综上所述,2种奶粉都能促进骨骼发育,而调节机制存在差异,这可能与不同奶粉配方的独特组成有关。

关键词：奶粉骨骼发育肠道菌群钙炎症因子

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Impact of dietary fat levels and fatty acid composition on milk fat synthesis in sows at peak lactation

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1134-1149页

作者： Li Zhe Uffe Krogh Charlotte Lauridsen Mette Olaf Nielsen zhengfeng fang Peter Kappel Theil Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationAnimal Nutrition InstituteSichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130China Department of Animal and Veterinary Sciences Aarhus UniversityFoulumDk‑8830 TjeleDenmark

Background Dietary fat is important for energy provision and immune function of lactating sows and their ***,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis,and milk fatty acid(FA)output is sparse in *** study aimed to evaluate impacts of dietary fat levels and FA composition on these traits in *** second-parity sows(Danish Landrace×Yorkshire)were assigned to 1 of 5 dietary treatments from d 108 of gestation until weaning(d 28 of lactation):low-fat control diet(3%added animal fat);or 1 of 4 high-fat diets with 8%added fat:coconut oil(CO),fish oil(FO),sunflower oil(SO),or 4%octanoic acid plus 4%FO(OFO).Three approaches were taken to estimate de novo milk fat synthesis from glucose and body *** Daily intake of FA was lowest in low-fat sows within fat levels(P<0.01)and in OFO and FO sows within highfat diets(P<0.01).Daily milk outputs of fat,FA,energy,and FA-derived carbon reflected to a large extent the intake of *** average,estimates for de novo fat synthesis were 82 or 194 g/d from glucose according to method 1 or 2 and 255 g de novo+mobilized FA/d according to method *** low-fat diet increased mammary FAS expression(P<0.05)and de novo fat synthesis(method 1;P=0.13)within fat *** OFO diet increased de novo fat synthesis(method 1;P<0.05)and numerically upregulated mammary FAS expression compared to the other high-fat *** diets,a daily intake of 440 g digestible FA minimized milk fat originating from glucose and mobilized body *** Sows fed diets with low-fat or octanoic acid,through upregulating FAS expression,increased mammary de novo fat synthesis whereas the milk FA output remained low in sows fed the low-fat diet or high-fat OFO or FO diets,indicating that dietary FA intake,dietary fat level,and body fat mobilization in concert determine de novo fat synthesis,amount and profiles of FA in milk.

关键词： Carbon metabolism De novo fat synthesis Dietary fatty acid Fat balance Mammary gene expression Mammary lipogenesis Milk fat production Piglet growth

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基于网络药理学和分子对接的半枝莲二萜类化合物抗肝癌作用机制

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安徽医药 2024年第3期28卷 475-482,I0003,I0004页

作者：严绪华梅格格梅凌方振峰武汉市中医医院综合药学部湖北武汉430010 江汉大学医学院药学系湖北武汉430056

目的基于网络药理学和分子对接技术探讨半枝莲二萜类成分抗肝癌的作用机制。方法根据文献调研(1987年1月至2022年7月)获得的155个半枝莲二萜类化合物,通过SwissADME平台筛选后得到符合条件的93种活性成分,并通过Swiss Target Predict... 详细信息

目的基于网络药理学和分子对接技术探讨半枝莲二萜类成分抗肝癌的作用机制。方法根据文献调研(1987年1月至2022年7月)获得的155个半枝莲二萜类化合物,通过SwissADME平台筛选后得到符合条件的93种活性成分,并通过Swiss Target Prediction网络平台预测得到其中65个化合物可能的作用靶点;通过GeneCards、OMIM、PharmGkb、TTD、DrugBank以及GEO、TCGA数据库获取与肝癌有关的靶点,利用Cytoscape软件构建可视化“活性成分-靶点-肝癌”网络作用图,并通过度值筛选主要活性成分;使用String平台进行蛋白质–蛋白质相互作用(PPI)网络功能富集分析,利用Cytoscape软件构建PPI可视化网络图,通过cytoHubba插件的评分规则筛选出核心靶点和关键靶点;采用R语言软件包对半枝莲二萜抗肝癌的作用靶点进行基因本体(GO)功能和京都基因和基因组数据库(KEGG)通路富集分析;使用AutoDock vina软件对主要活性成分与关键靶点进行对接分析。结果筛选出半枝莲二萜中6种活性成分包括Scutebarbolide I、Scutebata O、Scutelinquanine D、Scutellone F、Scutellone H和Scutebarbatine J,预测得到10个抗肝癌核心靶点,其中蛋白STAT3、MAPK1、MAPK3、HSP90AA1、PIK3R1和PIK3CA为关键靶点。基因富集分析得到953个GO功能条目和137条通路(P<0.05);分子对接结果显示,半枝莲二萜活性成分与关键靶点间存在潜在结合位点;网络药理学分析表明半枝莲二萜成分可能通过肽基-丝氨酸磷酸化、肽基-丝氨酸修饰、磷酸酶结合、蛋白质丝氨酸/苏氨酸激酶活性等生物学过程,作用于STAT3、MAPK1、MAPK3、PIK3R1、PIK3CA等关键蛋白的表达,进一步调控癌症中蛋白多糖、化学致癌受体激活、催乳素信号通路、癌症程序性死亡受体配体1(PD-L1)表达和程序性死亡受体1(PD-1)检查点通路等信号通路,发挥治疗肝癌作用。结论半枝莲二萜类化合物能够通过多分子、多靶点、多通路、多作用机制达到治疗肝癌作用。

关键词：半枝莲二萜类肝癌网络药理学分子对接作用机制

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长大裂隙控滑机制及斜坡破坏模式分析

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土工基础 2024年第1期38卷 112-116页

作者：张亚刘仕洪雷青方正峰姜波贵州省铜仁公路管理局贵州铜仁554399 贵州省交通规划勘察设计研究院股份有限公司贵阳550001

以甘龙滑坡为例,基于滑坡灾害与地质构造的实测和三维数值模拟,分析了长大裂隙控制下的斜坡破坏模式。结果表明:①该滑坡为长大裂隙控制的V型拉剪式滑坡;②滑坡启动后Ⅰ区中部滑体沿长大裂隙切割岩体的临空面顺层滑动,后缘山体受牵引拉... 详细信息

以甘龙滑坡为例,基于滑坡灾害与地质构造的实测和三维数值模拟,分析了长大裂隙控制下的斜坡破坏模式。结果表明:①该滑坡为长大裂隙控制的V型拉剪式滑坡;②滑坡启动后Ⅰ区中部滑体沿长大裂隙切割岩体的临空面顺层滑动,后缘山体受牵引拉裂破坏,滑体遇左缘陡壁的阻挡,随即向滑坡右缘转向,并推挤剪切Ⅰ区前缘坡体,堆积至河道呈“7”型分布;③随裂隙的贯通长度与深度增加,切割岩体长度与深度增加,岩体临空面增大,与层面组合形成的楔形体增大,导致滑坡的边界附近的位移峰值和变形区均不同程度的增加,一旦发育出的贯通滑面的裂隙,便会形成类似楔形体的滑坡。

关键词：长大裂隙滑坡三维数值模拟斜坡破坏模式

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Identification of Epsin1 as a regulator for hepatic lipid and glucose metabolism

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Genes & Diseases 2023年第1期10卷 72-75页

作者： Xiaohua Huang Long Jin zhengfeng fang Lianqiang Che Yan Lin Shengyu Xu Yong Zhuo Mingzhou Li De Wu Bin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengduSichuan 611130China Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education Sichuan Agricultural UniversityChengduSichuan 611130China College of Animal Science Sichuan Agricultural UniversityChengduSichuan 611130China

Obesity related non-alcoholic fatty liver disease(NAFLD),which can progress to steatohepatitis and insulin resistance,has become a major chronic liver disease worldwide.1 As an adaptor of clathrin-mediated endocytosis... 详细信息

关键词： metabolism healing wound

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Comparative effects of enzymatic soybean,fish meal and milk powder in diets on growth performance,immunological parameters,SCFAs production and gut microbiome of weaned piglets

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Journal of Animal Science and Biotechnology 2022年第2期13卷 567-577页

作者： Yingjie Li Yang Liu Jiangnan Wu Qiuhong Chen Qiang Zhou Fali Wu Ruinan Zhang zhengfeng fang Yan Lin Shengyu Xu Bin Feng Yong Zhuo De Wu Lianqiang Che Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Institute of Animal NutritionSichuan Agricultural UniversityNo.211Huimin RoadWenjiang DistrictChengdu 611130SichuanPeople’s Republic of China

Background:The objective of this study was to evaluate the replacement effects of milk powder(MK)and fish meal(FM)by enzymatic soybean(ESB)in diets on growth performance,immunological parameters,SCFAs production and gut microbiome of weaned ***:A total of 128 piglets with initial body weight at 6.95±0.46 kg,were randomly assigned into 4 dietary treatments with 8 replicates per treatment and 4 piglets per replicate for a period of 14 *** were offered isonitrogenous and iso-energetic diets as follows:CON diet with MK and FM as high quality protein sources,ESB plus FM diet with ESB replacing MK,ESB plus MK diet with ESB replacing FM,and ESB diet with ESB replacing both MK and ***:No significant differences were observed in growth performance among all treatments(P>0.05).However,piglets fed ESB plus FM or ESB diet had increased diarrhea index(P<0.01),and lower digestibility of dry matter(DM),gross energy(GE)or crude protein(CP),relative to piglets fed CON diet(P<0.01).Moreover,the inclusion of ESB in diet markedly decreased the plasma concentration of HPT and fecal concentration of butyric acid(BA)(P<0.01).The High-throughput sequencing of 16S rRNA gene V3−V4 region of gut microbiome revealed that the inclusion of ESB in diet increased the alpha diversity,and the linear discriminant analysis effect size(LEfSe)showed that piglets fed with ESB plus FM or ESB diet contained more gut pathogenic bacteria,such as g_Peptococcus,g_Veillonella and g_***:The inclusion of ESB in diet did not markedly affect growth performance of piglets,but the replacement of MK or both MK and FM by ESB increased diarrhea index,which could be associated with lower nutrients digestibility and more gut pathogenic ***,piglets fed diet using ESB to replace FM did not markedly affect gut health-related parameters,indicating the potential for replacing FM with ESB in weaning diet.

关键词： Enzymatic soybean Growth performance Gut microbiome Immunology SCFAs Weaned piglets

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Maternal organic selenium supplementation during gestation enhances muscle fiber area and muscle fiber maturation of offspring in porcine model

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Journal of Animal Science and Biotechnology 2023年第2期14卷 654-665页

作者： Yan Lin Hui Yan Lei Cao Daolin Mou Dajiang Ding Binting Qin Lianqiang Che zhengfeng fang Shengyu Xu Yong Zhuo Jian Li Jianping Wang Chao Huang Yuanfeng Zou Lixia Li De Wu Bin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengduChina Key Laboratory of Animal Disease‑Resistant Nutrition of Ministry of Education Sichuan Agricultural UniversityChengduChina College of Veterinary Medicine Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background:Organic selenium supplementation during gestation improves the antioxidant status and reproduc-tive performance of sows and increases the antioxidative capacity of the intestines of their *** study was conducted to investigate the effect of maternal basel diet(control)supplemented with an organic Se,2-hydroxy-4-methylselenobutanoic acid(HMSeBA),or inorganic sodium selenite(Na_(2)SeO_(3))during gestation on the antioxidant status and development of muscle in newborn and weaned *** piglets before colostrum intake and weaned piglets were selected for longissimus dorsi(LD)muscle collection and ***:The results showed that maternal HMSeBA supplementation increased the muscle area and content of Se in the LD muscle of newborn piglets,improved gene expression of selenoproteins,and decreased oxidative status in the LD muscle of both newborn and weaned piglets compared with the *** expression of muscle development-related genes of newborn piglets in the HMSeBA group was lower than in the control group,whereas the expression of MRF4 in weaned piglets was higher in the HMSeBA group than in the control and Na_(2)SeO_(3)*** addition,HMSeBA supplementation decreased the mRNA expressions of myosin heavy chains(MyHC)IIx and MyHC IIb and the percentage of MyHC IIb;increased the expression of PGC-1αin the LD muscle of newborn piglets;increased the gene expression of MyHC IIa;and decreased the protein expression of slow MyHC and the activity of malate dehydrogenase in the LD muscle of weaned piglets compared with the control ***:Maternal HMSeBA supplementation during gestation can improve the antioxidative capacity of the muscle of their offspring and promote the maturity of muscle fibres in weaned offspring.

关键词： Antioxidation HMSeBA Muscle Offspring Sows

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Microbial and metabolomic mechanisms mediating the effects of dietary inulin and cellulose supplementation on porcine oocyte and uterine development

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Journal of Animal Science and Biotechnology 2022年第3期13卷 907-922页

作者： Zhaoyue Men Meng Cao Yuechan Gong Lun Hua Ruihao Zhang Xin Zhu Lianchao Tang Xuemei Jiang Shengyu Xu Jian Li Lianqiang Che Yan Lin Bin Feng zhengfeng fang De Wu Yong Zhuo Animal Nutrition Institute Sichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130People’s Republic of China College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130People’s Republic of China

Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive *** objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms ***:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous *** diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted ***:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P0.1%)increased linearly or quadratically with increasing DF *** metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte ***:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic i

关键词： Dietary fiber Gilts Metabolomics Microbiota Oocyte maturation

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