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三疣梭子蟹附肢再生过程中qRT-PCR内参基因的筛选与Wnt7b表达研究应用

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核农学报 2025年第1期39卷 29-37页

作者：王思翔付媛媛翟伟郑霞刘磊王春琳宁波大学海洋学院浙江宁波315832 宁波海洋研究院浙江宁波315832

为筛选三疣梭子蟹(Portunus trituberculatus)附肢再生过程中的最适内参基因,以附肢再生不同阶段的三疣梭子蟹为研究对象,利用实时荧光定量PCR(qRT-PCR)对细胞骨架蛋白基因(β-actin)、甘油醛-3-磷酸脱氢酶基因(GAPDH)、18S rRNA(18S)... 详细信息

为筛选三疣梭子蟹(Portunus trituberculatus)附肢再生过程中的最适内参基因,以附肢再生不同阶段的三疣梭子蟹为研究对象,利用实时荧光定量PCR(qRT-PCR)对细胞骨架蛋白基因(β-actin)、甘油醛-3-磷酸脱氢酶基因(GAPDH)、18S rRNA(18S)、转录延伸因子基因(EF1α)、核糖体蛋白L18基因(RPL18)、细胞色素C氧化酶基因(COX)和组蛋白基因(HIS)共7个候选内参基因在肌肉、眼柄、血淋巴、肝胰腺、鳃、心脏和再生附肢共7种组织中的表达水平进行检测,并利用△Ct、geNorm、NormFinder和BestKeeper程序对候选内参基因的稳定性进行评价,筛选出合适的内参,最后以最适内参基因作为参考,分析再生相关基因Wnt7b的表达水平。结果显示,在不同组织中综合稳定性最好的是RPL18,而在不同再生阶段的再生附肢中β-actin和RPL18基因做双内参基因更适合。以RPL18和β-actin作双内参基因研究Wnt7b的表达水平时发现,Wnt7b基因在三疣梭子蟹附肢再生过程中的表达呈先上升后下降再上升的趋势;Wnt7b基因在三疣梭子蟹各组织中均有表达,除再生附肢和血淋巴外其他组织中的表达量都较低。本研究结果为甲壳类再生发育相关研究内参基因的选择及分子调控机制研究提供了参考。

关键词：三疣梭子蟹内参基因附肢再生实时荧光定量PCR

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保靖不同海拔黄金春茶品质形成主要气象及生化因子研究

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湖北农业科学 2021年第1期60卷 72-76页

作者：肖健刘慧郑福维刘松卿湘涛衡阳市气象局湖南衡阳421000 气象防灾减灾湖南省重点实验室长沙410118 湘西州气象局湖南吉首416000

利用2016—2018年保靖县黄金村不同海拔高度气象站资料,对保靖县不同海拔高度黄金茶生育期气象条件及影响春茶品质形成的主要生化因子特征进行了研究。结果表明,茶园山谷地带随海拔高度递增,温度递减0.3℃/100 m,年降水量递增106.2 mm/1... 详细信息

利用2016—2018年保靖县黄金村不同海拔高度气象站资料,对保靖县不同海拔高度黄金茶生育期气象条件及影响春茶品质形成的主要生化因子特征进行了研究。结果表明,茶园山谷地带随海拔高度递增,温度递减0.3℃/100 m,年降水量递增106.2 mm/100 m,年日照时数递增47 h/100 m;≥5℃有效积温对春茶开采有明显的指示意义;位于海拔360 m的保靖黄金茶主要生化因子含量最优,且优于其他知名茶叶;茶多酚含量与海拔高度、≥5℃有效积温和年日照时数呈正相关,相关系数均为0.98;酚氨比与上半年总降水量呈正相关(相关系数为0.94);咖啡因含量与海拔、年日照时数和≥5℃有效积温呈高度负相关(相关系数分别为-0.96、-0.96、-0.95)。

关键词：保靖黄金茶海拔气象因子茶叶品质

A Gohberg-Semencul Type Formula for the Inverse of Conjugate-Toeplitz Matrix and Applications

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Acta Mathematicae Applicatae Sinica 2018年第2期34卷 293-303页

作者： Yan-peng zheng Sugoog SHON Zun-wei fu School of Automation and Electrical Engineering Linyi University Linyi 276000 China School of Mathematical Sciences Qufu Normal University Qufu 273165 China Department of Computer Science the University of Suwon Hwaseong-si Gyeonggi-do 445-743 Korea

Constructing a kind of cyclic displacement, we obtain the inverse of conjugate-Toeplitz matrix by the aid of Gohberg-Semencul type formula. The stability of the inverse formula is discussed. Numerical examples are given to verify the feasibility of the inverse formula. We show how the analogue of our Gohberg-Semencul type formula leads to an efficient way to solve the conjugate-Toeplitz linear system of equations. It will be shown the number of real arithmetic operations is not more than known results. The corresponding conjugate-Hankel matrix is also considered.

关键词： conjugate-Toeplitz matrix cyclic displacement stability fast Fourier transform

Shear Banding Driven by Electric Field and Shear Flow

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Chinese Physics Letters 2013年第9期30卷 80-84页

作者： zheng Jie fu wei-Juan ZHOU Lu-wei State Key Laboratory of Surface Physics and Department of PhysicsFudan UniversityShanghai 200433

The shear banding is explored in a granular suspension driven by electric field and shear *** find that,given an imposed electric field,there is a critical shear rate determining whether shear banding emerges.A phase diagram of shear banding and fluidization is constructed by using the present experimental *** evolution in different shear banding phases constitutes an apparent shear thinning phenomenon,probably reflecting an underlying perspective of shear *** the two-phase model,combining with mean field approach and the Onsager least dissipation principle,we give a qualitative explanation of our experimental *** results suggest that the stress heterogeneity plays a crucial role for the physical origin of shear banding in this system.

关键词： flow. Shear qualitative

Influence of carbon addition on mechanical properties of Fe-Mn-C twinning-induced plasticity steels

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Journal of Iron and Steel Research International 2022年第9期29卷 1446-1454页

作者： Peng fu Zhi-bing zheng wei-ping Yang Hao-kun Yang School of Engineering Jiangxi Agricultural UniversityNanchang 330045JiangxiChina Institute of New Materials GuangdongAcademy of SciencesGuangdong Provincial iron Matrix Composite Engineering Research CenterGuangzhou 510650GuangdongChina Smart Manufacturing Division Hong Kong Productivity CouncilHong Kong 999077.China

Mechanical properties and microstructural evolution of Fe-22Mn-0.6C and Fe-22Mn-1.0C(wt.%)twinning-induced plasticity(TwIP)steels were investigated by monotonic,stress-relaxation and unloading-reloading tensile *** dynamic strain aging(DSA)effect,resulting from pinning of dislocations,effectively improved the dislocation activation volume of the two TWIP *** the meanwhile,DSA-facilitated twinning nucleation mechanism kept similar twinning capabilities of the two TWIP *** strain increasing,the formation of high-density deformation twins restricted the dislocation motion and reduced the activation volume with increasing ***,C addition simultaneously improved the ultimate tensile strength and uniform elongation,and significantly enhanced the friction stress,rather than back *** stronger short-range order effect,brought by friction stress,promotes the planar dislocation slipping,thus improving the work-hardening *** a result,the additional work-hardening capacity can be achieved in Fe-Mn-C with higher C addition.

关键词： Twinning induced plasticity steel Work-hardening rate Dynamic strain aging Short-range order Deformation twin

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政府债务审查监督:以人大为主体

学习论坛 2025年第1期 72-79页

作者：郑方辉梁伟湖广西大学公共管理学院广西南宁530004

以人大为主体审查监督政府债务是防范化解政府债务风险、健全现代债务管理制度、驱动政府债务管理向治理转变的重大举措。审视政府债务发展及管治历程,从政府内控管理向外部约束转变具有普遍性,体现价值理性和工具理性耦合。理论上,人... 详细信息

以人大为主体审查监督政府债务是防范化解政府债务风险、健全现代债务管理制度、驱动政府债务管理向治理转变的重大举措。审视政府债务发展及管治历程,从政府内控管理向外部约束转变具有普遍性,体现价值理性和工具理性耦合。理论上,人大主导的政府债务审查监督有利于形成协同监督合力、增强政府债务公信力、保障财政安全,具有法理依据及事实依据。建立报告制度、听取代表建议、引入第三方评价、完善法规条例构成人大审查监督政府债务的主要模式。推行人大主导政府债务“穿透式”监督,针对政府债务“举借、使用、管理、偿还”的全生命周期,是人大审查监督的内容指向。为此,应建立监督数字系统,加强监管协同,强化监督结果应用。

关键词：政府债务审查监督人大监督 “穿透式”监督

Study of thermal decoherence of harmonic oscillators

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原子与分子物理学报 2025年第5期42卷 108-114页

作者： LI Jian ZHOU Gao-Liang YAN zheng-Xin LIU wei WANG Zhao-Qi College of Science Xi’an University of Science and TechnologyXi’an 710054China

The thermal decoherence of harmonic oscillators is investigated *** quantum system presented here is a one-dimensional oscillator with angular frequency,which is surrounded by a thermal bath of environmental *** are various environmental oscillators with different angular frequency(below an ultraviolet cutoff).At the beginning,the quantum system is a pure state and the environmental oscillators are in thermodynamic equilibrium with *** a period,the system-environment interactions inspire significant decoherence of the quantum *** decoherence is displayed by explicit calculations of the purity and von Neumann entropy of the quantum *** is worth noting that the decoherence could be significant even in the weak coupling and low temperature case due to the large amount of environmental degrees of *** the decoherence process is inspired between the quantum system and an ordinary thermal environment here,the thermal decoherence result is quite general.

关键词： Quantum decoherence Grand canonical ensemble Oscillators

Optimization of preparation process of high-strength pellets from Panzhihua ilmenite concentrate

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Journal of Iron and Steel Research International 2023年第12期30卷 2391-2402页

作者： Gang-wei Zhou wei Lv fu-rong Chen zheng-gen Liu Man-sheng Chu Xue-wei Lv School of Metallurgy Northeastern UniversityShenyang110819LiaoningChina College of Materials Science and Engineering Chongqing UniversityChongqing400044China

The preparation of high-strength pellets from Panzhihua ilmenite concentrate is important for the hydrogen-rich smelting of titania slag and was *** effects of the magnetite concentrate and heating procedure including the pre-heating temperature and period as well as roasting temperature and period on the compressive strength of the roasted pellets were ***-strength ilmenite concentrate pellets(>2000 N)were prepared by optimizing the roasting *** addition of magnetite concentrate is beneficial for increasing the compressive strength of the Panzhihua ilmenite concentrate pellet by oxidizing hematite which filled the pseudobrookite particles,functioned as a bridge bond,and improved the interconnection between the *** compressive strength can reach up to 2900 N when the ilmenite concentrate pellet with 15%magnetite concentrate is pre-heated above 800℃ for 20 min and then roasted at 1250℃ for 20 *** addition,there is an overall upward trend in the compressive strength with an increase in the pre-heating temperature and period as well as the roasting temperature and *** micromorphology and strengthening consolidation mechanism of Panzhihua ilmenite concentrate pellets were also discussed.

关键词： Ilmenite concentrate Pelletizing Roasting Compressive strength-Pellet

Smart and fast reinforcement design for anti-dip bedding rock slopes

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第11期15卷 2943-2953页

作者： Yun zheng Congxin Chen Fei Meng Xiaodong fu wei Yuan State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan430070China Key Laboratory of Roads and Railway Engineering Safety Control of Ministry of Education Shijiazhuang Tiedao UniversityShijiazhuang050043China

Rock slopes are usually reinforced by a number of rock bolts due to the high efficiency and low ***,where should the rock bolts be installed is still a troublesome *** anti-dip bedding rock slopes(ABRSs),the installation position of rock bolts is a controlling factor that determines the reinforcement *** this work,a theoretical method is firstly proposed for assessing the stability of ABRSs reinforced by rock bolts using a limit equilibrium model.A comparison of theoretical calculations and numerical results was conducted to test the correctness of the theoretical *** on the stability assessment of ABRSs,we introduce adaptive moment estimation method(Adam)to optimize the installation location of rock *** Adam optimizer,the optimal layout of rock bolts with the maximum factor of safety can be determined,and the factor of safety of the slope increases by about 25%using the same amount of rock bolts but with different installation *** proposed method enables the fast stability analysis and supporting design for reinforced ABRSs,which paves the way to smart supporting design of slopes.

关键词： Rock slopes Toppling failure Rock bolts Stability assessment Smart analysis