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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long Xiao Gao zhengwei Wang Zhizhong Guan Xiaolan Qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

PtCu dendroframe octahedrons:Enhancing peroxidase-like activity and antibacterial efficiency through structural evolution

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Nano Research 2025年第1期18卷 100-106页

作者： Ri Feng Jiuyi Hu Liangjun Wang Jiayu Xu Jiajia Li Zeeshan Ahmad Shaohui zheng Muhammad Farhan Farid Wenjing Liu Faisal Saleem Key Laboratory of Flexible Electronics(KLOFE) Institute of Advanced Materials(IAM)and School of Flexible Electronics(Future Technologies)Nanjing Tech UniversityNanjing 211816China

Nanozymes,which possess enzyme-like catalytic activity,are attractive alternatives to natural *** activity is largely dependent on the structure of the ***,challenges remain in optimizing the structure-activity *** work explores the synthesis and utilization of PtCu nanostructures(NSs),focusing on their peroxidase(POD)-like properties and their potential as antibacterial *** developed PtCu dendrites that transform into unique dendroframe octahedrons(DFOs)and subsequently stabilize into solid octahedrons with unusual growth *** intermediate DFO,showed higher POD-like activity in comparison to other shapes of PtCu *** DFOs also have the better ability to consume glutathione,thus enhancing their sterilization ***,we demonstrate the effectiveness of these PtCu DFOs as powerful antibacterial *** work provides valuable information on shape dependent activities of PtCu NSs in the field of biomedicine.

关键词： nanozymes antibacterials peroxidase PtCu dendroframe octahedrons dendrites

Solubility and pharmacokinetics determination of chlorogeninc acid

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中国药理学与毒理学杂志 2015年第S1期29卷 113-114页

作者： LiZHANG Wei-shengXU Su-bowang Guan-huaDU YangLU Beijing City Key Laboratory of Polymorphic Drugs Research Centre of Polymorphic Drugs Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China Beijing City Key Laboratory of Drug Target and Screening Research National Center for Pharmaceutical Screening Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

OBJECTIVE To enhance the quality and efficiency of chlorogeninc acid by investigating the differences among the chlorogeninc acid polymorphs in bioavailability and *** Determinative method was used to analyze the solubility of chlorogeninc acid polymorphs;solid chlorogeninc acid in different forms were orally administered to the rats,and a HPLC method was established to determinate plasma lever of metabolite-acyclovir and the bioavailability was *** The indirect pharmacokinetic parameters of Chlorogeninc acid,as the metabolites of the form Ⅰ,Ⅱ,Ⅲ,were as follows:cmaxwas 0.37,0.34 and 0.44mg·L-1,respectively;AUC0→twas 0.71,0.76 and 0.79mg·L-1·h,*** The solubility of form Ⅲ was larger than the other forms′.The solubility of Chlorogeninc acid polymorphs:form Ⅰ,form Ⅱ,form Ⅲ were merely the same,there was no statistically significant difference in pharmacokinetic parameters among these three forms.

关键词： chlorogeninc acid polymorphs solubility pharmacoki

The compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one alleviates neuroinflammation and cognitive impairment in a mouse model of Alzheimer's disease

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Neural Regeneration Research 2025年第11期20卷 3330-3344页

作者： Xueyan Liu Wei Wu Xuejuan Li Chengyan Wang Ke Chai Fanru Yuan Huijuan zheng Yuxing Yao Chenlu Li Zu-Cheng Ye Daijun Zha Department of Medicinal Chemistry School of PharmacyFujian Medical UniversityFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Brain Aging and Neurodegenerative Diseases School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Institute of Laboratory Animal Center Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurosurgery Neurosurgery Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina Department of Hyperbaric Oxygen National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina

Previous studies have shown that the compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one(D30),a pyromeconic acid derivative,possesses antioxidant and anti-inflammatory properties,inhibits amyloid-β aggregation,and alleviates scopolamine-induced cognitive impairment,similar to the phase Ⅲ clinical drug *** this study,we established a mouse model of Alzheimer's disease via intracerebroventricular injection of fibrillar amyloid-β to investigate the effect of D30 on fibrillar amyloid-β-induced *** results showed that D30 alleviated fibrillar amyloid-β-induced cognitive impairment,promoted fibrillar amyloid-β clearance from the hippocampus and cortex,suppressed oxidative stress,and inhibited activation of microglia and astrocytes.D30 also reversed the fibrillar amyloid-β-induced loss of dendritic spines and synaptic protein ***,we demonstrated that exogenous fibrillar amyloid-βintroduced by intracerebroventricular injection greatly increased galectin-3 expression levels in the brain,and this increase was blocked by *** the role of D30 in clearing amyloid-β,inhibiting neuroinflammation,protecting synapses,and improving cognition,this study highlights the potential of galectin-3 as a promising treatment target for patients with Alzheimer's disease.

关键词： Alzheimer's disease amyloid-β astrocyte cognitive impairment D30 dendritic spines galectin-3 microglia neuroinflammation neuron

Luminescence: a new perspective for investigating perovskite precursors

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Science China Materials 2025年第1期 312-313页

作者： Haofeng zheng Shaocong Hou School of Electrical Engineering and Automation Wuhan University Wuhan University Shenzhen Research Institute

In the past two decades, perovskite materials have made significant progress in optoelectronic semiconductors, emerging as formidable candidates for the next generation of commercial solar cells, light-emitting diodes (LEDs), and *** the fundamental solution chemistry and photophysics in solution-processed perovskite devices is crucial for comprehending to achieve highly efficient optoelectronic devices.

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我国“高空抛坠物”侵权规则适用的三段证成——兼论《民法典》第1254条适用的司法范式

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海南大学学报（人文社会科学版） 2025年第1期43卷 142-150页

作者：郑志涛海南大学法学院海南海口570228

通过检视现行立法规范和实务适用逻辑,针对司法范式缺陷与适用困境,结合民法解释学,在大前提、小前提和结论三段结构中论证“高空抛坠物”侵权责任的法律适用,有助于提升司法裁判的可预期性。“小前提”系侵权案件事实,“大前提”系《... 详细信息

通过检视现行立法规范和实务适用逻辑,针对司法范式缺陷与适用困境,结合民法解释学,在大前提、小前提和结论三段结构中论证“高空抛坠物”侵权责任的法律适用,有助于提升司法裁判的可预期性。“小前提”系侵权案件事实,“大前提”系《民法典》第1254条之规定,“法律解释”系“大前提”和“小前提”之连结。将法律解释置于“大前提”和“小前提”之间,并推导“结论”的三段论证,有助正本溯源,统一类案之间法律适用。首先,由法律解释确定某法律规范是否适用;其次,通过法律解释规范“大前提”对“小前提”的理解和适用;最后,形成稳定的司法范式得出符合预期的“结论”。

关键词：高空抛坠物侵权责任公平责任

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Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-Min Qin Zhongliang Li Wen‑Xia Su Jia‑Min Hu Hanqin Zou Zhixuan Wu Zhiqin Ruan Yue‑Peng Cai Kang Li Qifeng zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

板柱节点考虑弯剪相关性的受冲切承载力计算方法

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哈尔滨工业大学学报 2025年第1期57卷 65-76页

作者：郑文忠吕盛先郑博文王英结构工程灾变与控制教育部重点实验室(哈尔滨工业大学) 哈尔滨150090 土木工程智能防灾减灾工业和信息化部重点实验室(哈尔滨工业大学) 哈尔滨150090

对于同时受到竖向荷载和不平衡弯矩作用的板柱节点,目前已有方法大多将不平衡弯矩等效为竖向荷载,从而给出其受冲切承载力计算方法。但该方法所涉及的不平衡弯矩比例系数的计算还存在较大争议,且没有考虑竖向荷载与不平衡弯矩耦合的影... 详细信息

对于同时受到竖向荷载和不平衡弯矩作用的板柱节点,目前已有方法大多将不平衡弯矩等效为竖向荷载,从而给出其受冲切承载力计算方法。但该方法所涉及的不平衡弯矩比例系数的计算还存在较大争议,且没有考虑竖向荷载与不平衡弯矩耦合的影响。因此,本文首先通过推导给出仅有竖向荷载作用的板柱节点受冲切承载力计算公式和仅有不平衡弯矩作用的板柱节点受弯承载力计算公式,并与试验结果进行对比,验证了所提公式的合理性。然后基于板柱节点在竖向荷载和不平衡弯矩共同作用下的试验结果,拟合得到板柱节点考虑弯剪相关性的受冲切承载力计算公式。最后对所提计算方法与不同规范计算方法进行对比,结果表明本文计算方法能够更加准确地预测板柱节点受冲切承载力。

关键词：板柱节点竖向荷载不平衡弯矩受冲切承载力计算方法

Effect of naked eukaryotic expression plasmid encoding rat augmenter of liver regeneration on acute hepatic injury and hepatic failure in rats

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World Journal of Gastroenterology 2005年第24期11卷 3680-3685页

作者： Li-MeiZhang Dian-WuLiu Jian-BoLiu Xiao-LinZhang Xiao-bowang Long-MeiTang Li-QinWang DepartmentofIntemalMedicine SecondPeople'sHospitalKunming650021YunnanProvinceChina DepartmentofEpidemiology HebeiMedicalUniversityShijiazhuang050017HebeiProvinceChina

AIM: To study the protective effect of eukaryotic expression plasmid encoding augmenter of liver regeneration (ALR) on acute hepatic injury and hepatic failure in rats. METHODS: The PCR-amplified ALR gene was recombined with pcDNA3 plasmid, and used to treat rats with acute hepatic injury. The rats with acute hepatic injury induced by intraperitoneal injection of 2 mL/kg 50% carbon tetrachloride (CCl4) were randomly divided into saline control group and recombinant pcDNA3-ALR plasmid treatment groups. Recombinant pcDNA3-ALR plasmid DNA (50 or 200 μg/kg) was injected into the rats with acute hepatic injury intravenously, intraperitoneally, or intravenously and intraperitoneally in combination 4 h after CCl4 administration, respectively. The recombinant plasmid was injected once per 12 h into all treatment groups four times, and the rats were decapitated 12 h after the last injection. Hepatic histopathological alterations were observed after HE staining, the expression of proliferating cell nuclear antigen (PCNA) in liver tissue was detected by immunohistochemical staining, and the level of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was determined by biochemical method. The recombinant plasmid DNA (200 μg/kg) and saline were intraperitoneally injected into the rats with acute hepatic failure induced by intraperitoneal injection of 4 mL/kg 50% CCl4 after 4 h of CCl4 administration, respectively. Rats living over 96 h were considered as ***: The sequence of ALR cDNA of recombinant pcDNA3-ALR plasmid was accordant with the reported sequence of rat ALR cDNA. After the rats with acute hepatic injury were treated with recombinant pcDNA3-ALR plasmid, the degree of liver histopathological injury markedly decreased. The pathologic liver tissues, in which hepatic degeneration and necrosis of a small amount of hepatocytes and a large amount of infiltrating inflammatory cells were observed, and they became basically normal in the most

关键词： ALR Acute hepatic injury Hepatic failure Gene therapy