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Organization and Ultra-Structural Components of Endothelial Surface Glycocalyx Revealed by Stochastic Optical Reconstruction Microscopy(STORM)

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医用生物力学 2019年第A1期34卷 6-7页

作者： Jie Fan yi Sun yifan Xia John M.Tarbell Bingmei M.fua Department of Biomedical Engineering the City College of the City University of New YorkNew YorkNY 10031USA Department of Electrical Engineering the City College of the City University of New YorkNew YorkNY 10031USA

Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood *** EC responses to these hemodynamic forces play a critical role in the homeostasis of the circulatory *** addition to forming a transport barrier between the blood and vessel wall,vascular ECs play important roles in regulating circulation *** biochemical stimuli,blood flow induced(hemodynamic)mechanical stimuli,such as shear stress,pressure and circumferential stretch,modulate EC morphology and functions by activating mechanosensors,signaling pathways,and gene and protein *** EC responses to the hemodynamic forces(mechano-sensing and transduction)are critical to maintaining normal vascular *** in the mechano-sensing and transduction leads to serious vascular diseases including hypertension,atherosclerosis,aneurysms and thrombosis,to name a few[1].On the luminal surface of our blood vessels,there is a thin layer called endothelial surface glycocalyx(ESG)which consists of proteoglycans,glycosaminoglycans(GAGs)and *** GAGs in the ESG are heparan sulfate(HS),hyaluronic acid(HA),chondroitin sulfate(CS),and sialic acid(SA)[2].In order to play important roles in vascular functions,such as being a mechanosensor and transducer for the endothelial cells(ECs)to sense the blood flow,a molecular sieve to maintain normal microvessel permeability and a barrier between the circulating cells and endothelial cells forming the vessel wall,the ESG should have an organized structure at the molecular *** to the limitations of optical and electron microscopy,the ultra-structure and organization of ESG has not been revealed until recent development of a super high resolution fluorescence optical microscope,STORM(Stochastic Optical Reconstruction Microscopy).The diffraction of a single fluorescence molecule can be described as the point spread function(PSF).When the light of wavelengt

关键词： Organization Ultra-Structural Components Endothelial Surface Glycocalyx Revealed Optical Reconstruction Microscopy STORM

High performance liquid chromatographic separation of thirteen drugs collected in Chinese Pharmacopoeia 2010(Ch.P2010) on cellulose ramification chiral stationary phase

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Journal of Pharmaceutical Analysis 2012年第1期2卷 48-55页

作者： ying Zhoua,b, Chao Maa, Yan Wanga, Qi-Ming Zhangb, yi-ying Zhanga, Jie fua, Hong Gaoc, Li-Xun Zhaoc aState Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China bNational Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China cInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China. National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China. Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China.

The enantiomers separation of thirteen drugs collected in Ch.P2010 was performed on chiral stationary phase of cellulose ramification (chiralpak OD and chiralpak OJ) by high performance liquid chromatographic (HPLC) methods, which included ibuprofen (C1), ketoprofen (C2), nitrendipine (C3), nimodipine (C4), felodipine (C5), omeprazole (C6), praziquantel (C7), propranolol hydrochloride (C8), atenolol (C9), sulpiride (C10), clenbuterol hydrochloride (C11), verapamil hydrochloride (C12), and chlorphenamine maleate (C13). The mobile phase consisted of isopropanol and n-hexane. The detection wavelength was set at 254 nm and the fiow rate was 0.7 mL/min. The enantiomers separation of these thirteen racemates on chiralpak OD column and chiralpak OJ column was studied, while the effects of proportion of organic additives, alcohol displacer and temperature on the separation were studied. And the mechanism of some of racemates was discussed. The results indicated that thirteen chiral drugs could be separated onchiral stationary phase of cellulose ramification in normal phase chromatographic system. The chromatographic retention and resolution of enantiomers could be adjusted by factors including column temperature and the concentration of alcohol displacer and organic alkaline modifier in mobile phase. It was shown that the resolution was improved with reducing concentration of alcohol displacer. When concentration of organic alkaline modifier was 0.2% (v/v), the resolution and the peak shape were fairly good. Most racemates mentioned above had better resolution at column temperature of 25 1C. When racemates were separated, the temperature should be kept so as to obtain stable separation results.

关键词： HPLC Chiral stationary phase Optical enantiomers Cellulose ramification

针对血清6型副黏病毒(APMV-6)快速荧光定量PCR检测方法的建立与初步应用

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中国兽医学报 2018年第4期38卷 645-649页

作者：陶志刘新鑫丁壮杨莹莹费亦东陈严玉李俊姣鲁承尹仁福延边大学农学院吉林延吉133000 吉林大学食品科学与工程学院吉林长春130062 吉林大学动物医学学院吉林长春130062

根据GenBank发表的6型副黏病毒（APMV6）参考毒株（KF267717．1）M基因序列，利用Oligo7设计合成1对引物，以重组阳性质粒为标准品，建立了APMV-6SYBRGreen实时荧光定量PCR检测方法。结果显示，建立的检测方法经优化后，线性关系良好，... 详细信息

根据GenBank发表的6型副黏病毒（APMV6）参考毒株（KF267717．1）M基因序列，利用Oligo7设计合成1对引物，以重组阳性质粒为标准品，建立了APMV-6SYBRGreen实时荧光定量PCR检测方法。结果显示，建立的检测方法经优化后，线性关系良好，标准曲线R^2=0．998，扩增效率107．322％。本方法敏感度高，特异性强，重复性好，最低检出拷贝数为2．78×10^2copies／μL，与NDV和APMV-4不发生特异性反应，比常规检测方法检出率高18％，可将其用于APMV-6的定量检测以及临床早期快速诊断。

关键词：禽副黏病毒6型 M基因高效荧光定量PCR

菊苣根水提物对正常大鼠糖脂尿酸代谢及肝肾安全性的影响研究

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世界中医药 2024年第13期19卷 1908-1915页

作者：胥亦王雨刘泽宇王雪安笑叶张冰北京中医药大学中药学院北京102488

目的:观察菊苣根水提物对正常大鼠糖、脂、尿酸代谢及肝肾安全性的影响。方法:SD大鼠随机分为空白对照组(水灌胃),菊苣高剂量、中剂量、低剂量组(10 mg/kg、5 mg/kg、2.5 mg/kg菊苣根水提物),别嘌醇组(20 mg/kg别嘌醇溶液),连续灌胃28 d... 详细信息

目的:观察菊苣根水提物对正常大鼠糖、脂、尿酸代谢及肝肾安全性的影响。方法:SD大鼠随机分为空白对照组(水灌胃),菊苣高剂量、中剂量、低剂量组(10 mg/kg、5 mg/kg、2.5 mg/kg菊苣根水提物),别嘌醇组(20 mg/kg别嘌醇溶液),连续灌胃28 d,动态检测实验大鼠血清葡萄糖(GLU)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血清尿酸(SUA)、尿尿酸(UUA)、粪便尿酸(fua)水平,并计算尿酸清除率(CUUA)及12 h尿尿酸排泄量;检测实验大鼠血清中尿酸代谢相关酶,如黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)水平;检测肝肾功能指标,如谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿素氮(BUN)、肌酐(Cr),并计算肝脏指数及肾脏指数。结果:实验期间,相较于空白对照组,菊苣各给药组大鼠糖、脂、尿酸代谢相关指标GLU、TC、TG、HDL-C、LDL-C、SUA、XOD、ADA、UUA、fua水平、CUUA、12 h尿尿酸排泄量,以及肝肾安全相关指标GPT、GOT、BUN、Cr、肝脏指数及肾脏指数均无明显差异。结论:菊苣根水提物不会影响正常大鼠糖、脂及尿酸代谢,且具有较好的肝肾安全性。

关键词：菊苣根水提物正常大鼠血清葡萄糖糖代谢脂代谢尿酸代谢肝肾安全性

聚焦超声消融治疗不同型别子宫腺肌病的临床疗效比较

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重庆医学 2022年第21期51卷 3623-3628,3633页

作者：赵易何玉春刘晓芳何佳遵义医科大学研究生院贵州遵义563006 四川省遂宁市中心医院妇产科 629000 四川省遂宁市中医院妇产科 629000

目的比较基于磁共振(MRI)特征不同型别子宫腺肌病聚焦超声消融(fua)治疗后的临床疗效。方法纳入2014年1月至2019年12月在遂宁市中心医院行fua治疗的子宫腺肌病患者227例,根据术前盆腔MRI检查,根据病灶位置将其分为4个亚型,其中Ⅰ型(内生... 详细信息

目的比较基于磁共振(MRI)特征不同型别子宫腺肌病聚焦超声消融(fua)治疗后的临床疗效。方法纳入2014年1月至2019年12月在遂宁市中心医院行fua治疗的子宫腺肌病患者227例,根据术前盆腔MRI检查,根据病灶位置将其分为4个亚型,其中Ⅰ型(内生型)82例,Ⅱ型(外生型)75例,Ⅲ型(结节型)34例,Ⅳ型(异质型)36例。对比分析4种亚型子宫腺肌病fua治疗结果及术后不良反应,对比术后3、6个月病灶体积变化情况、痛经及月经量情况。结果所有患者均在镇静镇痛下顺利完成fua治疗,按国防放射介入学会(SIR)分级发生A级不良反应51例(22.47%),B级不良反应3例(1.32%),无C~F级不良反应发生,各型A、B级不良反应的发生率比较差异无统计学意义(P>0.05)。Ⅲ型患者病灶消融率(NPVR)明显高于其他3型(P0.05)。治疗前后病灶体积变化:所有患者治疗后病灶体积较术前均有缩小,缩小率随时间增加逐渐增大。术后6个月,Ⅲ型患者病灶缩小率大于其他3型(P0.05)。月经量增多症状缓解情况:术后6个月,Ⅲ型患者的月经量增多症状总缓解率100%,而Ⅳ型患者的月经量增多症状总缓解率低于Ⅰ、Ⅱ、Ⅲ型(Pfua治疗不同类型子宫腺肌病是安全有效的,但不同腺肌病亚型间疗效存在差异,Ⅲ型子宫腺肌病患者行fua的治疗效果优于其他3型。

关键词：聚焦超声消融子宫腺肌病核磁共振分型治疗效果

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培元固本方联合聚焦超声消融治疗肝癌疗效观察

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湖北中医药大学学报 2023年第3期25卷 26-29页

作者：陈明明陈文直郭旭熊乙霓何佳超声医学工程国家重点实验室重庆医科大学重庆400016 四川省遂宁市中医院肿瘤科四川遂宁629000

目的观察培元固本方联合聚焦超声消融(fua)治疗肝癌疗效观察。方法将我院97例肝癌患者随机分为对照组48例与观察组49例。对照组仅给予fua治疗,观察组在对照组治疗基础上予培元固本方治疗。评价两组疗效,治疗前后检测两组血管内皮生长因... 详细信息

目的观察培元固本方联合聚焦超声消融(fua)治疗肝癌疗效观察。方法将我院97例肝癌患者随机分为对照组48例与观察组49例。对照组仅给予fua治疗,观察组在对照组治疗基础上予培元固本方治疗。评价两组疗效,治疗前后检测两组血管内皮生长因子(VEGF)、骨桥蛋白(OPN)、乏氧诱导因子-1α(HIF-1α)含量及两组糖类抗原125(CA125)、甲胎蛋白(AFP)、糖类抗原19-9(CA19-9)含量,两组天冬氨酸氨基转移酶(AST)、白蛋白(ALB)、丙氨酸氨基转移酶(ALT)含量。结果治疗后,观察组客观应答率高于对照组(Pfua治疗肝癌的效果确切,明显抑制肿瘤周围血管形成,降低肿瘤标志物含量,改善肝功能。

关键词：肝癌培元固本方聚焦超声消融