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Conservation of IRE1-Regulated bZIP74 mRNA Unconventional Splicing in Rice （Oryza sativa L.） Involved in ER Stress Responses

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Molecular Plant 2012年第2期5卷 504-514页

作者： Sun-Jie Lu zheng-ting yang Ling Sun Le Sun Ze-ting Song Jian-Xiang Li State Key Laboratory of Genetic Engineering Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai200433China

Protein folding in the endoplasmic reticulum （ER） is a fundamental process in plant cells that is vulnerable to many environmental stresses. When unfolded or misfolded proteins accumulate in the ER, the well-conserved unfolded protein response （UPR） is initiated to mitigate the ER stress by enhancing the protein folding capability and/or accelerating the ER-associated protein degradation. Here, we report the conservation of the activation mechanism of OsbZIP74 （also known as OsbZIP50）, an important ER stress regulator in monocot plant rice （Oryza sativa L.）. Under normal conditions, OsbZIP74 mRNA encodes a basic leucine-zipper transcription factor with a putative transmembrane domain. When treating with ER stress-inducing agents such as tunicamycin and DTT, the conserved double stem-loop structures of OsbZIP74 mRNA are spliced out. Thereafter, the resulting new OsbZIP74 mRNA produces the nucleus-localized form of OsbZIP74 protein, eliminating the hydrophobic region. The activated form of OsbZIP74 has transcriptional activation activity in both yeast cells and Arabidopsis leaf protoplasts. The induction of OsbZIP74 splicing is much suppressed in the OsIRE1 knock- down rice plants, indicating the involvement of OslRE1 in OsbZIP74 splicing. We also demonstrate that the unconventional splicing of OsbZIP74 mRNA is associated with heat stress and salicylic acid, which is an important plant hormone in systemic acquired resistance against pathogen or parasite.

关键词： mRNA unconventional splicing unfolded protein response bZIP transcription factor IRE1 heat stress salicylic acid (SA) Oryza sativa.

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The δ^(15)N values of foxtail millet(Setaria italica)and common millet(Panicum miliaceum)are reliable indicators of manuring practices

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Science China Earth Sciences 2024年第9期67卷 2910-2923页

作者： Jishuai yang Xiaoyan yang ting YOU Fahu CHEN Key Scientific Research Base of Bioarchaeology in Cold and Arid Regions(National Cultural Heritage Administration) Key Laboratory of Western China’s Environmental Systems(Ministry of Education)College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730000China Group of Alpine Paleoecology and Human Adaptation(ALPHA) State Key Laboratory of Tibetan Plateau Earth SystemResources and Environment(TPESRE)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000China

Millet agriculture,which originated in northern China,alongside rice agriculture,have nurtured the Chinese *** manuring practices likely promoted and maintained sustainable millet agricultural production in the loess area of northern ***,ongoing controversy exists regarding the indicators of prehistoric manuring intensity of foxtail millet(Setaria italica)and common millet(Panicum miliaceum).Here,we present the results of pot and field fertilization experiments on two millet *** findings suggest that manuring can significantly increase the δ^(15)N values of foxtail millet,and its δ^(15)N values increase with increasing manuring *** δ^(15)N values of foxtail millet leaves are systematically greater than those of grains by approximately 1.6‰.Manuring could have a long-term residual impact on increasing the δ^(15)N values of soil and millet *** with previous crop fertilization experiment results,we propose that the impact of manuring on the δ^(15)N values of non-nitrogen-fixing crops is roughly *** δ^(15)N value and amount of manure are key factors determining the extent of change in plant δ^(15)N *** millet grain δ^(15)N values can serve as reliable indicators of manuring ***,we provide an interpretive framework for assessing the correlation between manuring levels and the δ^(15)N values of archaeological millet *** δ^(15)N values of ancient millet grains suggest widespread and intensive manuring practices in prehistoric millet agriculture in northern China,spanning from the early yangshao period to the Longshan period.

关键词： Millet farming Manuring experiment Farming system Archaeobotany North China

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Ability of Helicobacter pylori to internalize into Candida

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World Journal of Gastroenterology 2024年第16期30卷 2281-2284页

作者： zheng-Hong Chen Jian-Chao Sun ting-Xiu yang Gu-Zhen Cui Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou Guizhou Medical UniversityGuiyang 561113Guizhou ProvinceChina Key Laboratory of Endemic and Ethnic Diseases of Ministry of Education Guizhou Medical UniversityGuiyang 561113Guizhou ProvinceChina Joint Laboratory of Helicobacter Pylori and Intestinal Microecology Affiliated Hospital of Guizhou Medical UniversityGuiyang 550009Guizhou ProvinceChina Department of Quality Control Guizhou Provincial Center for Clinical LaboratoryGuiyang 550002Guizhou ProvinceChina Department of Hospital Infection and Management Guizhou Provincial People’s HospitalGuiyang 550002Guizhou ProvinceChina

The following are our views regarding the“letter to the editor”(Helicobacter is preserved in yeast vacuoles!Does Koch’s postulates confirm it?)by Alipour and Gaeini,and the response“letter to the editor”(Candida accommodates nonculturable Helicobacter pylori in its vacuole-Koch’s postulates aren’t applicable)by Siavoshi and *** and Gaeini rejected the methods,results,discussion,and conclusions summarized in a review article by Siavoshi and *** present article reviews and discusses evidence on the evolutionary adaptation of Helicobacter pylori(***)to thrive in Candida cell vacuoles and concludes that Candida could act as a Trojan horse,transporting potentially infectious *** into the stomach of humans.

关键词： Helicobacter pylori Candida yeast Intracellular presence Helicobacter pylori-specific gene Helicobacter pylori transmission

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Normal sperm head morphometric reference values in fertile Asian males

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Asian Journal of Andrology 2024年第3期26卷 315-320页

作者： Ye-Lin Jia Ying-Bi Wu Lin Yu Yan zheng ting-ting yang Yan-Yun Wang Bin Zhou Lin Zhang Fu-Ping Li Laboratory of Andrology West China Second University Hospital Human Sperm BankChengdu 610000China Laboratory of Molecular Translational Medicine Center for Translational MedicineChengdu 610061China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationChengdu 610061China NHC Key Laboratory of Chronobiology West China Second University HospitalSichuan UniversityChengdu 610000China

Sperm head morphology is crucial for male factor infertility diagnosis and assessment of male reproductive *** are available to analyze sperm head morphology,but they are limited by poor methodology comparability and *** study aimed to explore comprehensive and new normal morphometric reference values for spermatozoa heads infertile Asian *** automated sperm morphology analysis system captured 23152 stained spermatozoa from confirmed *** these samples,1856 sperm head images were annotated by three experienced laboratory technicians as“normal”.Weemployed 14 novel morphometric features to describe sperm head size(head length,head width,length/width ratio,and girth),shape(ellipse intersection over union,girth intersection over union,short-axis symmetry,and long-axis symmetry),area(head,acrosome,postacrosomal areas,and acrosome area ratio),and degrees of acrosome and nuclear *** straight-forwardmethod for the morphometric analysis of sperm by accurate visual measurements is clinically *** measured parameterspresent valuable information to establish morphometric reference intervals for normal sperm heads in fertile Asian *** detailed measurement data will be valuable for interlaboratory comparisons and technician *** vitro fertilizationand andrology laboratory technicians can use these parameters to perform objective morphology evaluation when assessing malefertilization potential.

关键词： fertile Asian males head morphometric reference value normal spermatozoa

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Stability and accuracy of central difference method for real-time dynamic substructure testing considering mass participation coefficient

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Earthquake Engineering and Engineering Vibration 2024年第3期23卷 625-636页

作者： zheng Lichang Xu Guoshan yang Ge Wang Zhen yang Kaibo zheng Zhenyun School of Civil Engineering Harbin Institute of TechnologyHarbin 150090China Key Lab of Structures Dynamic Behavior and Control Ministry of EducationHarbin Institute of TechnologyHarbin 150090China Key Lab of Intelligent Disaster Mitigation Ministry of Industry and Information TechnologyHarbin 150090China School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan 430070China

For real-time dynamic substructure testing(RTDST),the influence of the inertia force of fluid specimens on the stability and accuracy of the integration algorithms has never been ***,this study proposes to investigate the stability and accuracy of the central difference method(CDM)for RTDST considering the specimen mass participation ***,the theory of the CDM for RTDST is ***,the stability and accuracy of the CDM for RTDST considering the specimen mass participation coefficient are ***,numerical simulations and experimental tests are conducted for verifying the effectiveness of the *** study indicates that the stability of the algorithm is affected by the mass participation coefficient of the specimen,and the stability limit first increases and then decreases as the mass participation coefficient *** most cases,the mass participation coefficient will increase the stability limit of the algorithm,but in specific circumstances,the algorithm may lose its *** stability and accuracy of the CDM considering the mass participation coefficient are verified by numerical simulations and experimental tests on a three-story frame structure with a tuned liquid damper.

关键词： real-time dynamic substructure testing central difference method stability mass participation coefficient tuned liquid damper

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The Short-Duration Heavy Rainfall in Different Quadrants of Northeast China Cold Vortices

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Journal of Meteorological Research 2024年第2期38卷 321-338页

作者： Lei yang Yongguang zheng Liaoning Meteorological Disaster Monitoring and Early Warning Center Shenyang 110166 National Meteorological Centre China Meteorological AdministrationBeijing 100081 China Meteorological Administration Tornado Key Laboratory Foshan 528315

The Northeast China cold vortex(NCCV)is one of the main synoptic-scale systems causing short-duration heavy rainfall(SDHR)in Northeast *** conditions(e.g.,water vapor,instability,and vertical wind shear)are known to be distinctly different over the four quadrants of NCCVs,rendering prediction of the SDHR related to NCCVs(NCCV_SDHR)more *** on 5-yr hourly rainfall observations from 3196 automatic weather stations and ERA5 reanalysis data,10,232 NCCV_SDHR events were identified and divided into four quadrant groups according to their relative position to the center of the NCCV(CVC).The results show that the southeast quadrant features the highest frequency of SDHR,with stronger intensity,longer duration,and wider coverage;and the SDHR in different quadrants presents different formation mechanisms and varied temporal evolution.A new coordinate system is established relative to the CVC that uses the CVC as the origin and the radius of the NCCV(r CV)as the unit *** this new coordinate system,all of the NCCV_SDHR events in the 5-yr study period are *** is found that the occurrence frequency of NCCV_SDHR initially increases and then decreases with increasing distance from the *** highest frequency occurs mainly between 0.8 and 2.5 times r CV from the CVC in the southeast *** can be attributed to the favorable conditions,such as convergence of the low-level shear line and abundant water vapor,which are concentrated in this ***,high-frequency NCCV_SDHR larger than 50 mm(NCCV_SDHR50)is observed to be closer to the *** NCCV_SDHR50occurs,the NCCV is in closer proximity to the subtropical high,resulting in stronger low-level convergence and more abundant water ***,there are lower lifting condensation levels and stronger 0-6-and 0-1-km vertical wind shears in these *** findings provide a valuable reference for more accurate prediction of NCCV_SDHR.

关键词： Northeast China cold vortex short-duration heavy rainfall statistical characteristics environmental conditions

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A review on graphite surface modification methods towards low carbon-containing refractories

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Journal of Iron and Steel Research International 2024年第6期31卷 1291-1303页

作者： ting-ting Zhang Jun Ding Bei-yue Ma zheng-long Liu Chao Yu Cheng-ji Deng The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina School of Metallurgy Northeastern UniversityShenyang 110819LiaoningChina

The rapid development of iron and steel metallurgy technology has promoted the continuous innovation and iteration of carbon-containing refractories for clean steel *** meet the high-quality requirements for clean steel production and full exploit the performance advantages of carbon-containing refractories in dynamic smelting environment,it is necessary to explore the role of graphite and modified graphite in carbon-containing *** on this,graphite surface modification methods,including surfactants,surface oxidation,and surface coating,and their applications in carbon-containing refractories are *** advantages and disadvantages of each method are analyzed for practical ***,combined with the existing problems,the application prospect of improved graphite in carbon-containing refractories is discussed.

关键词： Graphite Carbon-containing refractory Clean steel Surface modification Service performance

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Enhanced carbon capture with motif-rich amino acid loaded defective robust metal-organic frameworks

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Nano Research 2024年第3期17卷 2004-2010页

作者： Qi-Ye Ju Jia-Jia zheng Li Xu Hai-Yan Jiang Zi-Qian Xue Lu Bai yang-yang Guo Ming-Shui Yao ting-Yu Zhu State Key Laboratory of Multi-phase Complex Systems CAS Key Laboratory of Green Process and EngineeringBeijing Key Laboratory of Ionic Liquids Clean ProcessInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Laboratory of Theoretical and Computational Nanoscience National Center for Nanoscience and TechnologyChinese Academy of SciencesBeijing 100190China School of Rare Earths University of Science and Technology of ChinaHefei 230026China Ganjiang Innovation Academy Chinese Academy of SciencesGanzhou 341000China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Institute for Integrated Cell-Material Sciences Kyoto UniversityInstitute for Advanced StudyKyoto UniversityYoshidaUshinomiya-choSakyokuKyoto 606-8501Japan

The use of metal-organic frameworks(MOFs)as solid adsorption materials for carbon capture is promising,but achieving efficient and reversible adsorption with a balance of capacity and selectivity for carbon dioxide(CO_(2))over N_(2) remains a *** take full advantage of the strong channel traffic and robustness of MOFs with relatively small pores,it is highly necessary to employ a defect-engineering strategy to construct a broader channel structure that can facilitate the loading of functional motif-rich amino acids(AAs).This strategy can greatly enhance the CO_(2) adsorption performance of *** this study,motif-rich amino acids are loaded into the defective and robust porous frameworks via combined defect-engineering and post-synthetic *** defective Zr/Hf-MOF-808s modified with AAs,especially for the 18 mol%4-nitroisophthalic acid,generated defective products allowing for the loading of L-serine(L-Ser).This modification resulted in a significant improvement in both the adsorption capacity(248%improvement at 298 K,100 kPa)and the selectivity of CO_(2)/N_(2) using the ideal adsorbed solution theory(IAST),with the selectivity increasing to 120.55 and 38.27 at 15 and 100 kPa,respectively,while maintaining good cycling *** functional theory(DFT)simulation,CO_(2) temperature-programmed desorption(CO_(2)-TPD),and in situ Fourier transform infrared spectroscopy(FTIR)were further employed to have a better understanding of the enhanced CO_(2) adsorption ***,unlike the AAs loaded pristine MOF-808s that showed the best CO_(2) adsorption capacity with the loading of short and small glycine(Gly),the broadened channel size in our work enables the loading of functional motif-rich L-serine,which brings more active binding sites,improving CO_(2) adsorption.

关键词： carbon capture and storage metal-organic framework(MOF) amino acids(AAs) defect-engineering motif-rich

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Recent progress in functional modification and crosslinking of bioprosthetic heart valves

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Regenerative Biomaterials 2024年第1期3卷 1-16页

作者： Cheng zheng Li yang Yunbing Wang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China

Valvular heart disease(VHD),clinically manifested as stenosis and regurgitation of native heart valve,is one of the most prevalent cardiovascular diseases with high *** valve replacement surgery has been recognized as golden standard for the treatment of *** to the clinical application of transcatheter heart valve replacement technic and the excellent hemodynamic performance of bioprosthetic heart valves(BHVs),implantation of BHVs has been increasing over recent years and gradually became the preferred choice for the treatment of ***,BHVs might fail within 10-15 years due to structural valvular degeneration(SVD),which was greatly associated with drawbacks of glutaraldehyde crosslinked BHVs,including cytotoxicity,calcification,component degradation,mechanical failure,thrombosis and immune *** prolong the service life of BHVs,much effort has been devoted to overcoming the drawbacks of BHVs and reducing the risk of *** this review,we summarized and analyzed the research and progress on:(i)modification strategies based on glutaraldehyde crosslinked BHVs and(ii)nonglutaraldehyde crosslinking strategies for BHVs.

关键词： bioprosthetic heart valves modification crosslinking anticalcification antithrombosis

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Demonstration of acousto-optical modulation based on a thin-film AlScN photonic platform

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Photonics Research 2024年第6期12卷 1138-1149页

作者： KEWEI BIAN ZHENYU LI YUSHUAI LIU SUMEI XU XINGYAN ZHAO yang QIU YUAN DONG QIZE ZHONG TAO WU SHAONAN zheng ting HU School of Microelectronics Shanghai UniversityShanghai 201800China School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China

Acousto-optic(AO)modulation technology holds significant promise for applications in microwave and optical signal ***-film scandium-doped aluminum nitride(AlScN),with excellent piezoelectric properties and a wide transparency window,is a promising candidate for achieving on-chip AO modulation with a fabri-cation process compatible with complementary metal-oxide-semiconductor(CMOS)*** study presents,to the best of our knowledge,the first demonstration of AO modulators with surface acoustic wave generation and photonic waveguides monolithically integrated on a 400-nm-thick film of AlScN on an *** intramodal AO modulation is realized based on an AlScN straight waveguide,and the modulation efficiency is significantly enhanced by 12.3 dB through the extension of the AO interaction length and the utilization of bidirectional acoustic *** intermodal AO modulation and non-reciprocity are further demonstrated based on a multi-mode spiral waveguide,achieving a high non-reciprocal contrast(>10 dB)across an optical bandwidth of 0.48 *** research marks a significant stride forward,representing an advancement in the realization of microwave photonic filters,magnet-free isolators,and circulators based on the thin-film AlScN photonic platform.

关键词： waveguide film microwave

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