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Effects of Adipose-derived Mesenchymal Stem Cell Exosomes on Corneal Stromal Fibroblast Viability and Extracellular Matrix Synthesis

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Chinese Medical Journal 2018年第6期131卷 704-712页

作者： Ting Shen Qing-Qing zheng jiang Shen Qiu-Shi Li Xing-Hui Song Hong-Bo Luo Chao-Yang Hong Ke Yao Eye Institute of Zhejiang University Eye Center of Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou Zhejiang 310009 China Department of Ophthalmology Zhejiang Provincial People's Hospital and People's Hospital of Hangzhou Medical College Hangzhou Zhejiang 310014 China School of Ophthalmology and Optometry Wenzhou Medical University Wenzhou Zhejiang 325035 China Facility for Biochemistry and Molecular Medicine Core Facilities School of Medicine Zhejiang University Hangzhou Zhejiang 310058 China

Background： Corneal stromal cells （CSCs） are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells （ADSCs） have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear. Methods： ADSCs and CSCs were isolated from New Zealand white rabbits and cultured in vitro. An Exosome Isolation Kit, Western blotting, and nanoparticle tracking analysis （NTA） were used to isolate and confirm the exosomes from ADSC culture medium. Meanwhile, the optimal exosome concentration and treatment time were selected. Cell Counting Kit-8 and annexin V-fluorescein isothiocyanate/ propidium iodide assays were used to assess the effect of ADSC-derived exosomes on the proliferation and apoptosis of CSCs. To evaluate the effects ofADSC-derived exosomes on CSC invasion activity, Western blotting was used to detect the expression of matrix metalloproteinases （MMPs） and collagens. Results： ADSCs and CSCs were successfully isolated from New Zealand rabbits. The optimal concentration and treatment time of exosomes for the following study were 100 μg/ml and 96 h, respectively. NTA revealed that the ADSC-derived exosomes appeared as nanoparticles （40-200 nm）, and Western blotting confirmed positive expression of CD9, CDSI, flotillin-1, and HSP70 versus ADSC cytoplasmic proteins （all P〈 0.01 ）. ADSC-derived exosomes （50μg/ml and 100μg/ml） significantly promoted proliferation and inhibited apoptosis （mainly early apoptosis） of CSCs versus non-exosome-treated CSCs （all P 〈 0.05）. Interestingly, MMPs were downregulated and extracellular matrix （ECM）-related proteins including collagens and fibronectin were upregulated in the exosome-treated CSCs versus non-

关键词： Adipose-derived Mesenchymal Stem Cell Corneal Stromal Cells Exosomes Extracellular Matrix Synthesis

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A drug-eluting Balloon for the trEatment of coronarY bifurcatiON lesions in the side branch:a prospective multicenter ranDomized (BEYOND)clinical trial in China

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Chinese Medical Journal 2020年第8期133卷 899-908页

作者： Quan-Min Jing Xin Zhao Ya-Ling Han Ling-Ling Gao Yang zheng Zhan-Quan Li Ping Yang Hong-Liang Cong Chuan-Yu Gao Tie-Min jiang Hui Li Jun-Xia Li Dong-Mei wang Geng wang Zhan-Chun Cong Zhong Zhang Department of Cardiology General Hospital of Northern Theater CommandShenyangLiaoning 110016China Peking University Clinical Research Institute Beijing 100191China Department of Cardiology The First Bethune Hospital of Jilin UniversityChangchunJilin 130021China Department of Cardiology The People’s Hospital of Liaoning ProvinceShenyangLiaoning 110016China Department of Cardiology China-Japan Union Hospital of Jilin UniversityChangchunJilin 130033China Department of Cardiology Tianjin Chest HospitalTianjin 300051China Department of Cardiology Henan Provincial People’s HospitalZhengzhouHenan 450008China Department of Cardiology Affiliated Hospital of Logistics University of People’s Armed Police ForceTianjin 300163China Department of Cardiology Daqing Oilfield General HospitalDaqingHeilongjiang 163001China Department of Cardiology The Seventh Medical Center of People’s Liberation Army General HospitalBeijing 100125China Department of Cardiology Bethune International Peace HospitalShijiazhuangHebei 050082China Department of Cardiology The 306th Hospital of People’s Liberation ArmyBeijing 100101China

Background:Treatment of coronary bifurcation lesions remains challenging;a simple strategy has been preferred as of late,but the disadvantage is ostium stenosis or even occlusion of the side branch(SB).Only a few single-center studies investigating the combination of a drug-eluting stent in the main branch followed by a drug-eluting balloon in the SB have been *** prospective,multicenter,randomized study aimed to investigate the safety and efficacy of a paclitaxel-eluting balloon(PEB)compared with regular balloon angioplasty(BA)in the treatment of non-left main coronary artery bifurcation ***:Between December 2014 and November 2015,a total of 222 consecutive patients with bifurcation lesions were enrolled in this study at ten Chinese *** were randomly allocated at a 1:1 ratio to a PEB group(n=113)and a BA group(n=109).The primary efficacy endpoint was angiographic target lesion stenosis at 9 *** efficacy and safety endpoints included target lesion revascularization,target vessel revascularization,target lesion failure,major adverse cardiac and cerebral events(MACCEs),all-cause death,cardiac death,non-fatal myocardial infarction,and thrombosis in target *** main analyses performed in this clinical trial included case shedding analysis,base-value equilibrium analysis,effectiveness analysis,and safety *** version 9.4 was used for the statistical ***:At the 9-month angiographic follow-up,the difference in the primary efficacy endpoint of target lesion stenosis between the PEB(28.7%±18.7%)and BA groups(40.0%±19.0%)was-11.3%(95%confidence interval:-16.3%to-6.3%,Psuperiority<0.0001)in the intention-to-treat analysis,and similar results were recorded in the per-protocol analysis,demonstrating the superiority of PEB to *** lumen loss was significantly lower in the PEB group than in the BA group(-0.06±0.32 vs.0.18±0.34 mm,P<0.0001).For intention-to-treat,there were no significant differences bet

关键词： Coronary bifurcation lesions Drug-eluting balloon Target lesion stenosis Late lumen loss

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Increased regional homogeneity in internet addiction disorder： a resting state functional magnetic resonance imaging study

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Chinese Medical Journal 2010年第14期123卷 1904-1908页

作者： LIU Jun GAO Xue-ping Isoken Osunde LI Xin ZHOU Shun-ke zheng Hui-rong LI Ling-jiang Institute of Mental-Health Department of Radiology the Second Xiangya Hosipital Central South University Changsha Hunan 410011 China Wayne State University School of Medicine Detroit MI 48202 USA

Background Internet addition disorder （lAD） is currently becoming a serious mental health problem among Chinese adolescents. The pathogenesis of lAD, however, remains unclear. The purpose of this study applied regional homogeneity （ReHo） method to analyze encephalic functional characteristic of lAD college students under resting state. Methods Functional magnetic resonanc image （fMRI） was performed in 19 lAD college students and 19 controls under resting state. ReHo method was used to analyze the differences between the average ReHo in two groups. Results The following increased ReHo brain regions were found in lAD group compared with control group： cerebellum, brainstem, right cingulate gyrus, bilateral parahippocampus, right frontal lobe （rectal gyrus, inferior frontal gyrus and middle frontal gyrus）, left superior frontal gyrus, left precuneus, right postcentral gyrus, right middle occipital gyrus, right inferior temporal gyrus, left superior temporal gyrus and middle temporal gyrus. The decreased ReHo brain regions were not found in the lAD group compared with the control group. Conclusions There are abnormalities in regional homogeneity in lAD college students compared with the controls and enhancement of synchronization in most encephalic regions can be found. The results reflect the functional change of brain in lAD college students. The connections between the enhancement of synchronization among cerebellum, brainstem, limbic lobe, frontal lobe and apical lobe may be relative to reward pathways.

关键词： internet addiction disorder resting state regional homogeneity functional magnetic resonance image

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Diagnostic performance of magnetic resonance venography in the detection of recanalization in patients with chronic cerebral venous sinus thrombus

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Chinese Medical Journal 2009年第20期122卷 2428-2432页

作者： SUN Ying zheng Dong-you JI Xun-ming Peter WEALE WU Hao jiang Li-dan YANG Li-zhuang Department of Neurosurgery The 2nd Affiliated Hospital of Harbin Medical University Harbin 150086 China Department of Anesthesiology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Neurosurgery Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Radiology Xuanwu Hospital Capital Medical University Beijing 100053 China Siemens Medical Solutions USA Inc. Chicago IL 60611 USA

Background In the chronic stage of cerebral venous sinus thrombosis （CVST）, recanalization can result in disparate MR appearances. We aimed to prospectively investigate the diagnostic accuracy of magnetic resonance venography （MRV） in the evaluation of the recanalization of CVST. Methods This study prospectively evaluated the diagnostic performance of 2-dimensional time-of-flight （2D-TOF） MRV in thirty-two consecutive patients during a threeto six-month follow-up for CVST. Both 2D-TOF MRV and digital substraction angiography （DSA） were undertaken. Diagnostic accuracy of 2D-TOF MRV in the detection of recanalized thrombus was evaluated using DSA as the reference standard. Results MRV and DSA were completed without complications in all 32 patients. The sensitivity, specificity, positive predictive value, and negative predictive value of 2D-TOF MRV for the detection of recanalization on a segmental basis were 91% （62/68）, 93% （37/40）, 95% （62/65）, and 86% （37/43） respectively. Conclusion 2D-TOF MRV provides high sensitivity and specificity for the diagnosis of recanalized CVST segments.

关键词： venous sinus thrombosis, cranial magnetic resonance venography angiography, digital subtraction

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超声破裂载基因微泡增强心肌细胞报告基因的转染与表达

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中国应用生理学杂志 2005年第4期21卷 371-375,i0009页

作者：汪国忠胡申江郑哲岚孙坚李江郑霞朱朝晖姚宇玫浙江大学医学院附属第一医院心内科杭州310003 浙江大学医学院附属第一医院超声影像科杭州310003

目的:通过超声破裂载基因微泡介导报告基因心肌细胞转染,探讨其能否增强心肌细胞外源基因转染与表达。方法:以-βgalactosidase质粒为报告基因,将其与自制氟碳气体微泡粘附,制备载基因微泡。利用诊断性超声破裂微泡进行体外心肌细胞基... 详细信息

目的:通过超声破裂载基因微泡介导报告基因心肌细胞转染,探讨其能否增强心肌细胞外源基因转染与表达。方法:以-βgalactosidase质粒为报告基因,将其与自制氟碳气体微泡粘附,制备载基因微泡。利用诊断性超声破裂微泡进行体外心肌细胞基因转染;以磷酸钙共沉淀转染为阳性对照并将其以不同方式与超声破裂微泡技术联合应用,以期进一步增强基因转染效果。分别采用原位染色及酶学定量检测-βgalactosidase表达水平,同时进行细胞活性检测。结果:超声破裂载基因氟碳气体微泡(PESDA)转染组心肌细胞-βgalactosidase表达水平可达单纯质粒转染组60倍(P<0.01)。磷酸钙共沉淀转染3.67倍(P<0.01)超声强度、微泡浓度对超声破裂介导基因转染效果有明显影响。超声破裂微泡技术与磷酸钙共沉淀联合应用可进一步提高报告基因的表达(P<0.05),即使在磷酸钙转染后6 h,超声破裂微泡仍能明显增强报告的基因的表达(P<0.05)。结论:超声破裂微泡技术是一种高效基因转染方法,其不但能增加DNA转染,而且增强入胞后基因的表达。超声破裂微泡与其它基因转染技术联合应用能进一步增加基因转染效率。

关键词：超声造影剂基因心肌细胞

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Statistical analyses of metric data of hominoid teeth found in Yuanmou of China

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Chinese Science Bulletin 2000年第10期45卷 936-942页

作者： LIU Wu, zheng Liang & jiang Chu1. Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China 2. Institute of Archaeology of Yunnan Province, Kunming 650118, China 3. Yuanmou Man Museum, Yuanmou 651300, China Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing China Institute of Archaeology of Yunnan Province Kunming China Yuanmou Man Museum Yuanmou China

Mesial-distal and buccal-lingual dimensions of 1 266 hominoid teeth found in Yuanmou of Yunnan Province during the period of 1986 and 1988 have been measured. The statistical analyses of the metric data show that the tooth sizes of Yuanmou hominoid can be divided into big and small two types, and most tooth dimensions display obvious bimodalities in both histograms and 2 dimensional plots. The coefficients of variance (CV) reveal that the CVs of Yuanmou hominoid tooth sizes are within or close to the CV ranges of Lufengpithecus and extant great apes. The comparisons of tooth sizes and their proportions indicate that Yuanmou hominoids have close affinities with Lefeng and Kaiyuan hominoids, and have obvious differences with Gorilla, Chimpanzee and Orangutan. The authors believe that the hominoid fossils found in Yuanmou in the past 10 years represent a single species with highly sexual dimorphism in morphology and this species may have a close evolutionary relationship with the hominoids found in

关键词： human origin Yuanmou hominoid dental metrics statistical analyses.

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一类关联混沌系统的分时切换混沌同步

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物理学报 2008年第2期57卷 709-713页

作者：刘扬正姜长生林长圣南京航空航天大学自动化学院南京210016 南京工程学院非线性物理研究所南京211167

提出关联混沌系统分时切换混沌同步的思想.利用线性反馈控制方法实现一类关联混沌系统中各个子系统与其复制系统间的分时切换混沌同步.根据时变系统的稳定性理论,得到了使各子系统都能实现混沌同步的反馈控制增益的取值范围.数值仿真实... 详细信息

提出关联混沌系统分时切换混沌同步的思想.利用线性反馈控制方法实现一类关联混沌系统中各个子系统与其复制系统间的分时切换混沌同步.根据时变系统的稳定性理论,得到了使各子系统都能实现混沌同步的反馈控制增益的取值范围.数值仿真实验证明了理论分析的正确性和可行性.

关键词：分时切换混沌同步线性反馈关联混沌系统

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Fabrication and characterization of ZnO/Se1‑xTex solar cells

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Frontiers of Optoelectronics 2022年第3期15卷 75-85页

作者： Jiajia zheng Liuchong Fu Yuming He Kanghua Li Yue Lu Jiayou Xue Yuxuan Liu Chong Dong Chao Chen jiang Tang Wuhan National Laboratory for Optoelectronics(WNLO)and School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China China-EU Institute for Clean and Renewable Energy(ICARE) Huazhong University of Science and TechnologyWuhan 430074China

Selenium(Se)element is a promising light-harvesting material for solar cells because of the large absorption coefcient and prominent ***,the efciency of Se solar cells has been stagnated for a long time owing to the suboptimal bandgap(>1.8 eV)and the lack of a proper electron transport *** this work,we tune the bandgap of the absorber to the optimal value of Shockley-Queisser limit(1.36 eV)by alloying 30%Te with 70%***,ZnO electron transport layer is selected because of the proper band alignment,and the mild reaction at ZnO/Se_(0.7)Te_(0.3) interface guarantees a good-quality ***,a superior efciency of 1.85%is achieved on ZnO/Se_(0.7)Te_(0.3)solar cells.

关键词： Se1−xTex alloy ZnO electron transport layer Recombination mechanism Solar cells

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功能性调驱微球的制备及稳定性研究

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当代化工 2024年第8期53卷 1905-1908页

作者：肖丽华宋鑫吕鹏鲍文博董宏超蒋志伟郑旭林李文涛杜若飞中海石油(中国)有限公司天津分公司天津300452 东北石油大学提高采收率重点实验室黑龙江大庆163318

渤海油田已形成了微球调驱、分级组合深部调剖、在线组合调驱等多项具有渤海特色的调驱技术,通过对产出液中调驱体系的精准检测,可进一步提高调驱过程动态调整的精准度,提升多轮次方案设计精度。但目前受微球材料、检测方法的限制,难以... 详细信息

渤海油田已形成了微球调驱、分级组合深部调剖、在线组合调驱等多项具有渤海特色的调驱技术,通过对产出液中调驱体系的精准检测,可进一步提高调驱过程动态调整的精准度,提升多轮次方案设计精度。但目前受微球材料、检测方法的限制,难以较好实现微球调驱产出液的精准检测。针对此问题,提出选用在制备功能性调驱微球过程中加入荧光量子点,然后和壳层单体水液混合吸附的方法制备功能性调驱微球,并对其稳定性进行分析。结果表明:制备的功能性调驱微球具有良好的荧光特性,并且随着老化温度、老化时间、模拟水矿化度的变化,仍表现出较好的稳定性。

关键词：渤海油田聚合物微球稳定性机理分析

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局部外加钢筋网水泥砂浆面层加固砌体结构试验研究

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施工技术 2014年第10期43卷 41-45页

作者：郑昊蒋璐姜浩上海市建筑科学研究院(集团)有限公司上海200032 上海市工程结构新技术重点实验室上海200032

制作了2个1∶2砌体结构整体模型,其中1个采用局部外加钢筋网水泥砂浆面层加固,另1个为已有圈梁-构造柱体系砌体结构模型,进行了低周往复加载对比试验。通过对比2个模型的滞回特性、骨架曲线、延性系数、耗能能力以及刚度退化等指标的变... 详细信息

制作了2个1∶2砌体结构整体模型,其中1个采用局部外加钢筋网水泥砂浆面层加固,另1个为已有圈梁-构造柱体系砌体结构模型,进行了低周往复加载对比试验。通过对比2个模型的滞回特性、骨架曲线、延性系数、耗能能力以及刚度退化等指标的变化规律发现,采用局部外加钢筋网水泥砂浆面层法加固后的砌体结构具有良好的抗震性能与较好的延性,且该方法加固量小,附加影响少,是一种较为理想的加固方法,具有较高的推广价值。

关键词：砌体结构钢筋网水泥砂浆面层低周往复加载抗震加固

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