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Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat

Journal of Integrative Agriculture 2020年第5期19卷 1170-1185页

作者： fang zheng-wu HE Yi-qin LIU Yi-ke JIANG Wen-qiang SONG Jing-han WANG Shu-ping MA Dong-fang YIN Jun-liang Hubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of AgricultureYangtze UniversityJingzhou 434025P.R.China Institute of Food Crops Hubei Academy of Agricultural SciencesWuhan 430064P.R.China Institute of Plant Protection Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management of Crop in Southwest ChinaMinistry of Agriculture and Rural AffairsChengdu 610066P.R.China

Non-specific lipid transfer proteins(nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development. Recently, nsLTPs have been shown to respond to different forms of abiotic stresses. Despite the vital roles of nsLTPs in many plants, little is known about the nsLTPs in wheat. In this study, 330 nsLTP proteins were identified in wheat and they clustered into five types(1, 2, c, d, and g) by phylogenetic analysis with the nsLTPs from maize, Arabidopsis, and rice. The wheat nsLTPs of type d included three subtypes(d1, d2, and d3) and type g included seven subtypes(g1–g7). Genetic structure and motif pattern analyses showed that members of each type had similar structural composition. Moreover, GPI-anchors were found to exist in non-g type members from wheat for the first time. Chromosome mapping revealed that all five types were unevenly and unequally distributed on 21 chromosomes. Furthermore, gene duplication events contributed to the proliferation of the nsLTP genes. Large-scale data mining of RNA-seq data covering multiple growth stages and numerous stress treatments showed that the transcript levels of some of the nsLTP genes could be strongly induced by abiotic stresses, including drought and salinity, indicating their potential roles in mediating the responses of the wheat plants to these abiotic stress conditions. These findings provide comprehensive insights into the nsLTP family members in wheat, and offer candidate nsLTP genes for further studies on their roles in stress resistance and potential for improving wheat breeding programs.

关键词： nsLTP abiotic stress wheat genome bioinformatics gene family

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Exploring the effect of in vitro digestion on the phenolics and antioxidant activity of Lycium barbarum fruit extract

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Food Bioscience 2023年第1期51卷 391-396页

作者： Yi-Long Ma Yue Wang zheng-fang wu Jie Mei Wen-Qing Zhang Ya-fang Shang Kiran Thakur Zhao-Jun Wei School of Food and Biological Engineering Hefei University of TechnologyHefei230009China Collaborative Innovation Center for Food Production and Safety School of Biological Science and EngineeringNorth Minzu UniversityYinchuan750021China Research Institute of Jiangnan Small Pit Brewing Technology XuanjiuXuancheng242000China

To assess the effect of the digestive system on the phenolics and antioxidant capacity of Lycium barbarum fruit,its extract was digested and analyzed in *** results revealed that the phenolics/flavonoids contents and antioxidant capacity in the pH-treated group and digested group generally decreased by 4.39%-21.22%in the oral and intestinal digestions but increased by 1.26%-16.14%in the gastric *** phenolic content and antioxidant capacity were highly correlated in digested groups(r>0.899,p<0.01).Five main phenolics including rutin,p-coumaric acid and its hexoside derivatives were identified and quantified,and these phenolics displayed higher stability during the digestion,implying that they may be the potential functional phenolics of *** fruit.

关键词： Lycium barbarum Phenolics Antioxidant capacity In vitro digestion

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AZ61镁合金降温多向压缩中的动态析出及其对晶粒细化和力学性能的影响

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中国有色金属学报 2018年第2期28卷 223-232页

作者：方正午肖振宇张笃秀尹勇杨续跃中南大学材料科学与工程学院长沙410083 中南大学有色金属先进结构材料与制造协同创新中心长沙410083 中南大学有色金属学材料科学与工程教育部重点实验室长沙410083

对AZ61镁合金进行降温多向压缩变形,利用OM,SEM/EBSD及TEM技术对其晶粒细化和析出相变化进行了观察和分析。结果表明:在623~483 K温度范围内降温多向压缩时AZ61镁合金晶粒急剧细化,低角晶界通过吸收位错转变成高角晶界,而降温变形会明... 详细信息

对AZ61镁合金进行降温多向压缩变形,利用OM,SEM/EBSD及TEM技术对其晶粒细化和析出相变化进行了观察和分析。结果表明:在623~483 K温度范围内降温多向压缩时AZ61镁合金晶粒急剧细化,低角晶界通过吸收位错转变成高角晶界,而降温变形会明显抑制晶粒长大和晶界高角化,5道次变形后还存在大量中低角晶界,平均晶粒尺寸约为0.8μm。变形初期析出的第二相尺寸较大,随着变形道次的增加,第二相细化的同时数量明显增多。降温变形可促进动态析出,使得析出相分布更加细小、弥散。动态析出使得AZ61相对AZ31镁合金具有更高的硬度。AZ61镁合金经降温变形后强度和延性得到同步提升,尤其是5道次变形后伸长率显著提高,这是细小第二相在中低角晶界上动态析出造成的。

关键词：镁合金多向压缩中低角度晶界动态析出

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转录因子SCX和MKX在肌腱发育和分化中的研究进展

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中国细胞生物学学报 2016年第12期38卷 1549-1554页

作者：吴震张子旺王晓艾郑莉灵吴芳浙江省立同德医院杭州310012 浙江大学医学院杭州310058 浙江大学医学院附属儿童医院杭州310003

肌腱损伤是临床的常见病之一,其愈合后易发生黏连而影响肌腱功能,因此,有效地改善和促进愈合、恢复肌腱功能成为亟待解决的主要问题。为求在根源上找到解决问题的方法,众多学者对肌腱发育的机制进行了大量的研究,发现了肌腱发育和分化... 详细信息

肌腱损伤是临床的常见病之一,其愈合后易发生黏连而影响肌腱功能,因此,有效地改善和促进愈合、恢复肌腱功能成为亟待解决的主要问题。为求在根源上找到解决问题的方法,众多学者对肌腱发育的机制进行了大量的研究,发现了肌腱发育和分化的关键转录因子Scleraxis(SCX)和Mohawk(MKX)。为了更好地了解肌腱发育机制,该文主要对转录因子SCX和MKX的发现、在肌腱中的分布和功能以及参与的信号通路等方面进行了阐述。

关键词：转录因子 SCX MKX FGFs信号通路 TGFβ信号通路

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Intermittent High Glucose Reduces Androgen Receptor Expression of HUVECs via PI3K/Akt/mTOR signaling Pathway

Intermittent High Glucose Reduces Androgen Receptor Expressi...

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中华医学会第十一次全国内分泌学学术会议

作者： Lin Li,XUEYAO YIN,FENGPING zheng,fang wu,JIAQIANG ZHOU,HONG LI Department of Endocrinology,the Affiliated Sir Run Run Shaw Hospital,School of Medicine,Zhejiang University

Objective To elucidate the effect of intermittent high glucose on androgen receptor(AR) expression and the PI3K/Akt/ roTOR signaling pathway in human umbilical vein endothelial cells(HUVECs). Methods HUVECs were cultured in endothelial cell growth media(ECM) with supplemental fetal bovine serum(5%),endothelial cell growth supplement(IX),penicillin(10U/mL) and streptomycin(10μg/mL).HUVECs were incubated in media with different glucose concentrations:5.5mmol/L(N),30mmol/ L(H),or alternating 5.5 and 30mmol/1 glucose twice a day(F)

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Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： Xiahong Tang Nan zheng Qingming Lin Yan You zheng Gong Yangping Zhuang Jiali wu Yu Wang Hanlin Huang Jun Ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

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小麦品种中梁16的抗条锈性研究

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西南农业学报 2015年第1期28卷 136-139页

作者：马东方方正武孙正祥彭菲鲁红学长江大学农学院湖北荆州434025 主要粮食作物产业化湖北省协同创新中心湖北荆州434025 西北农林科技大学/旱区作物逆境生物学国家重点实验室陕西杨凌712100

小麦条锈病是小麦生产上最严重的世界性病害之一。小麦品种中梁16具有抗逆性强、高产、抗条锈性强等优良特性。为明确其抗条锈性遗传规律,利用条锈菌小种CYR30对中梁16与感病品种铭贤169及其杂交后代进行苗期抗条锈性遗传分析。结果表明... 详细信息

小麦条锈病是小麦生产上最严重的世界性病害之一。小麦品种中梁16具有抗逆性强、高产、抗条锈性强等优良特性。为明确其抗条锈性遗传规律,利用条锈菌小种CYR30对中梁16与感病品种铭贤169及其杂交后代进行苗期抗条锈性遗传分析。结果表明,中梁16对CYR30小种具有良好的抗性,由1对显性基因控制,暂命名为Yr Zhong16。通过分子标记分析,获得了与Yr Zhong16连锁的4个SSR标记Xwmc696、Xgwm644、Xbarc95和Xgwm131。其中与Yr Zhong16最近的侧翼位点为Xgwm644和Xbarc95,其遗传距离分别是2.3和3.5 c M。根据SSR标记的定位结果,将Yr Zhong16定位在小麦染色体7BL上。这些与Yr Zhong16连锁的分子标记为利用中梁16抗条锈病基因进行抗病基因聚合和分子标记辅助育种奠定了基础。

关键词：中梁16 小麦条锈病抗病基因遗传分析分子标记

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Sine oculis homeobox homolog family function in gastrointestinal cancer:Progression and comprehensive analysis

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World Journal of Clinical Oncology 2025年第1期16卷 10-24页

作者： Yang-zheng Lan zheng wu Wen-Jia Chen Xin-Ning Yu Hua-Tao wu Jing Liu Department of The Breast Center Cancer Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of General Surgery First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

The sine oculis homeobox homolog(SIX)family,a group of transcription factors characterized by a conserved DNA-binding homology domain,plays a critical role in orchestrating embryonic development and organogenesis across various organisms,including *** six distinct members,from SIX1 to SIX6,each member contributes uniquely to the development and differentiation of diverse tissues and organs,underscoring the versatility of the SIX *** or mutations in SIX genes have been implicated in a spectrum of developmental disorders,as well as in tumor initiation and progression,highlighting their pivotal role in maintaining normal developmental trajectories and cellular *** to target the transcriptional complex of the SIX gene family have emerged as a promising strategy to inhibit tumor *** the development of inhibitors targeting this gene family is still in its early stages,the significant potential of such interventions holds promise for future therapeutic ***,this review aimed to comprehensively explore the advancements in understanding the SIX family within gastrointestinal cancers,focusing on its critical role in normal organ development and its implications in gastrointestinal cancers,including gastric,pancreatic,colorectal cancer,and hepatocellular *** conclusion,this review deepened the understanding of the functional roles of the SIX family and explored the potential of utilizing this gene family for the diagnosis,prognosis,and treatment of gastrointestinal cancers.

关键词： Sine oculis homeobox homolog Gastrointestinal cancer Transcription factor Development Regulation Diagnosis Therapeutics

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小麦穗发芽鉴定方法的比较与分析

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广西植物 2016年第3期36卷 261-266页

作者：李玉营马东方王晓玲方正武长江大学农学院主要粮食作物产业化湖北省协同创新中心湖北荆州434025

穗发芽是小麦生产中较为严重的灾害之一,易受外界环境的影响,一旦发生不仅会影响产量,而且还会严重影响小麦的品质,因此培育抗穗发芽的小麦品种至关重要。该研究通过对65份小麦材料进行穗发芽试验,比较分析了小麦穗发芽抗性的常用方法,... 详细信息

穗发芽是小麦生产中较为严重的灾害之一,易受外界环境的影响,一旦发生不仅会影响产量,而且还会严重影响小麦的品质,因此培育抗穗发芽的小麦品种至关重要。该研究通过对65份小麦材料进行穗发芽试验,比较分析了小麦穗发芽抗性的常用方法,即籽粒发芽法、整穗发芽法和大田穗发芽法。结果表明:三种方法之间均呈极显著正相关关系,而且在1%水平上均存在极显著性差异;发芽指数与籽粒发芽率的相关性最高,能够更好地评价小麦材料的休眠特性,但不能得出材料的总体抗性;籽粒发芽法和整穗发芽法的变异程度相对较小,试验条件更易控制,可作为小麦穗发芽抗性评价的简易方法;多数参试材料的平均籽粒发芽率>平均整穗发芽率>平均大田穗发芽率,且三者差异程度均达到极显著水平,这说明麦穗的外部结构及外部环境对小麦穗发芽的影响显著。因此,籽粒发芽法可以从休眠性方面,对小麦种子资源进行初步筛选;整穗发芽法可用于穗发芽抗性的进一步鉴定和验证,评价小麦材料穗发芽的综合抗性;大田穗发芽法较易受自然条件的影响、变异程度较大,其结果可以作为室内发芽试验的参考数据。

关键词：小麦穗发芽穗发芽鉴定

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甜荞半胱氨酸蛋白酶FaRDL基因的克隆和序列结构分析

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湖北农业科学 2013年第22期52卷 5609-5611页

作者：方正武景雄刘志雄长江大学作物遗传育种研究所湖北荆州434025 长江大学园艺园林学院湖北荆州434025

同源克隆结合RACE(Ropid amplification of cDNA ends)技术,从甜荞(Fagopyrum esculentum)花芽中克隆到了半胱氨酸蛋白酶FaRDL基因cDNA序列(GenBank登录号为JN605352)。结果表明,其cDNA全长1 298 bp,包括1个编码362个氨基酸共1 089 bp的开放阅读框(Open reading frame,ORF)。其蛋白与拟南芥中木瓜蛋白酶GCP1的同源性最高,属半胱氨酸蛋白酶家族中的木瓜蛋白酶亚家族,有1个由22个氨基酸残基组成的信号肽、1个由100个氨基酸残基组成的前体肽和1个由Cys149-His285-Asn305木瓜蛋白酶家族保守的催化三联体活性位点。

关键词：甜荞(Fagopyrum esculentum) 半胱氨酸蛋白酶 FaRDL基因克隆

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