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“蛋白质化学”的课程思政教学设计与探讨

生命的化学 2024年第1期44卷 177-182页

作者：陈燕马利刚郑晓珂河南中医药大学药学院郑州450046

课程思政是我国高等教育探索实践的热点之一。生物化学作为中药学专业的必修课程,对学生的知识体系构建、能力培养和价值塑造具有重要的影响。本文以生物化学课程中“蛋白质化学”教学内容为例,从爱国情怀、团结协作、勇于创新、遵纪守... 详细信息

课程思政是我国高等教育探索实践的热点之一。生物化学作为中药学专业的必修课程,对学生的知识体系构建、能力培养和价值塑造具有重要的影响。本文以生物化学课程中“蛋白质化学”教学内容为例,从爱国情怀、团结协作、勇于创新、遵纪守法、职业道德等方面深入挖掘知识点相关的思政元素;并以“蛋白质结构与功能”为例,介绍了课程思政融入教学过程的探索,旨在为生物化学的课程思政建设提供参考。

关键词：课程思政生物化学蛋白质化学

基于非靶向代谢组学研究腺苷对阿尔兹海默症的干预作用

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中国医院药学杂志 2024年第9期44卷 1007-1015页

作者：陈龙周宁王瑞锋曾梦楠冯卫生郑晓珂河南中医药大学药学院河南郑州450046 河南省中药开发工程技术研究中心河南郑州450046 河南中医药大学呼吸疾病中医药防治省部共建协同创新中心河南郑州450046

目的:基于代谢组学方法研究腺苷对Aβ25-35多肽诱导的阿尔兹海默症(Alzheimer’s disease,AD)小鼠体内代谢紊乱的干预作用。方法:将小鼠随机分为对照组、模型组、阳性药组、腺苷组。脑室内注射Aβ25-35多肽模拟AD的生理病理状态,末次给... 详细信息

目的:基于代谢组学方法研究腺苷对Aβ25-35多肽诱导的阿尔兹海默症(Alzheimer’s disease,AD)小鼠体内代谢紊乱的干预作用。方法:将小鼠随机分为对照组、模型组、阳性药组、腺苷组。脑室内注射Aβ25-35多肽模拟AD的生理病理状态,末次给药结束,禁食12 h后收集各组小鼠的尿液样本。处理后的尿液样本进行超高效液相色谱-四极杆-飞行时间质谱(UHPLC-Q/TOF-MS)分析,得到质谱原始数据,经预处理后导入SIMCA-P软件,进行主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)筛选潜在差异代谢物,并利用HMDB、MassBank等在线数据库进一步鉴定生物标志物。通过Mev软件对生物标志物在各组中的相对含量进行热图聚类分析。结合MBRole、KEGG和MetaboAnalyst5.0等在线数据库进行通路富集分析和代谢网络构建。结果:腺苷组小鼠代谢轮廓与模型组明显分离,并向正常组回调。腺苷组与模型组间共筛选出59个差异标志物(正源模式下38个、负源模式下21个),通路富集分析后得到烟酸盐和烟酰胺代谢、精氨酸和脯氨酸代谢、精氨酸生物合成、苯丙氨酸代谢、半胱氨酸和蛋氨酸代谢等显著受干扰的代谢通路。结论:腺苷可能通过氨基酸代谢和维生素代谢来修复AD小鼠的神经损伤,为临床上腺苷治疗AD的应用提供了理论依据。

关键词：腺苷阿尔兹海默症代谢组学超高效液相色谱-四极杆-飞行时间质谱

Recent Climate Trends on the Northern Slopes of the Tianshan Mountains, Xinjiang, China

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Journal of Mountain Science 2009年第3期6卷 255-265页

作者： ZHANG Hongfeng OUYANG Zhiyun zheng Hua WANG xiaoke State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China

In arid regions, mountains fulfill important ecological and economic functions for the surrounding lowlands. In the scenario of global warming, mountain ecosystems change rapidly, especially in the arid region of northwestern China. This paper provides an assessment of the changes in temperature and precipitation in the historical records of climate on the northern slopes of the eastern Tianshan Mountains. A Mann-Kendall nonparametric trend and Sen＇s tests are employed to analyze the interannual changes and innerannual variability in temperature and precipitatiofi in the regions of low to high altitude. The present study finds that the largest increases in annual temperature are observed at stations in the low altitude regions. The significant increasing trends in temperature tend to occur mainly in late winter and early spring at stations from middle to high altitude, but in summer and autumn at stations of low altitudes. The increasing trends in annual precipitation are found from the middle to high altitude areas, but decreasing trends are found in the low altitude areas. The significant increasing trends in precipitation occur mostly in winter and earlier spring at stations from the middle to high altitudes, while the increasing and decreasing trend coexists at stations of low altitude with most of the significant trend changes occurring in March, June and August.

关键词： Mountainous region Climate change Temperature Precipitation China

Association between the fMRI manifestations of activated brain areas and muscle strength in patients with space-occupying lesions in motor cortex

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Neural Regeneration Research 2006年第4期1卷 347-350页

作者： Wenbin zheng xiaoke Chen Guorui Liu Renhua Wu Department of Radiology Second Affiliated Hospital ShantouUniversity Medical CollegeShantou 501041 GuangdongProvince China

BACKGROUND ： Functional magnetic resonance imaging （fMRI） studies have disclosed the changes of the motor function in the motor cortex of ipsilateral and contralateral hemispheres of tumor, which have special significance for making the surgical planning and most greatly minimizing the postoperative functional damages. OBJECTIVE： To analyze the association between the manifestation characteristics of hand functional area and motor dysfunction using fMRI in patients with space-occupying lesions of tumor in motor cortex. DESIGN ： A case-controlled observation SETTING： Department of Radiology, Second Affiliated Hospital, Shantou University Medical College .PARTICIPANTS： Twenty-three patients （13 males and 10 females） with space-occupying lesions of central sulcus area, aged 21-53 years with a mean age of （47±1） years were selected from the Second Affiliated Hospital of Shantou University Medical College. All the patients were diagnosed by MR scanning as space-occupying lesions of motor area, and pathologically confirmed that the lesions involved central sulcus and central Iobule; Lesions occurred in left and right hemispheres in 13 and 10 cases respectively. The tumor types were astrocytoma （n =8）, metastatic tumours （n =7）, meningiomas （n =5） and oligodendroglioma （n =3）. The muscle strength was normal in 11 cases （grade 5） and obviously decreased in 12 cases （grade 2-3 in 3 cases and grade 4 in 9 cases）; muscle strengths of both upper and lower limbs were decreased in 7 cases, and only that of upper limbs was decreased in 5 cases. Informed consents were obtained from all the subjects. Meanwhile, 9 healthy physical examinees （5 males and 4 females） of 20-56 years old with an average of （34±1） years were taken as controls. All the patients and healthy subjects were right-handed. METHODS： All the enrolled subjects were examined with MR scanning and functional imaging. Twenty cases whose clinical symptoms were mild in the patient group and 9 healthy volunteers

关键词： fMRI Association between the fMRI manifestations of activated brain areas and muscle strength in patients with space-occupying lesions in motor cortex

Different meridian tropism in three Chinese medicines: Tinglizi(Semen Lepidii Apetali), Yiyiren(Semen Coicis), Cheqianzi(Semen Plantaginis)

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Journal of Traditional Chinese Medicine 2019年第2期39卷 213-220页

作者： Zeng Mengnan Li Miao Zhang Li Zhang Beibei Wu Guangcao Feng Weisheng zheng xiaoke Department of Pharmacy Henan University of Chinese Medicine Department of Pharmacy Henan University of Chinese Medicine Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province

OBJECTIVE: To study the meridian tropism(MT) for three Chinese medicines: Tinglizi(Semen Lepidii Apetali, TLZ), Yiyiren(Semen Coicis, YYR), Cheqianzi(Semen Plantaginis, CQZ) in vivo ***: The extracts of TLZ,YYR, CQZ were orally administered to rats at a dose 234, 700 and 350 mg/kg, respectively. The urinary excretion rate, loss in weight of rats and the electrolyte levels in the plasma were measured, in order to assess the mechanisms of the three traditional Chinese diuretic ***: TLZ, YYR and CQZ exhibited obvious diuretic effect and reduced the weight of saline-loaded animals. All the three diuretic medicines differentially reduced various serum, urinary, lung, stomach, kidney and hormonal markers, thus indicating the different mechanisms of action and a close resemblance with their different meridian as per the MT ***: Our findings suggest that the three Traditional Chinese Medicine(TCM) medicines-TLZ,YYR and CQZ-possess different Shamisen *** can support the regimen of treating the same disease with different therapies in TCM theory.

关键词： Medicine,Chinese traditional Diuretics Meridian Tropism

3D Printing of Well Dispersed Electrospun PLGA Fiber Toughened Calcium Phosphate Scaffolds for Osteoanagenesis

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Journal of Bionic Engineering 2020年第4期17卷 652-668页

作者： Guoru Zhao Rongwei Cui You Chen Sijie Zhou Chen Wang Zhangmei Hu xiaoke zheng Maohong Li Shuxin Qu Key Lab of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Analytical and Testing Center Southwest Jiaotong UniversityChengdu 610031China Key Lab of High-speed Railway Engineering Ministry of EducationSchool of Civil EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Although the toughening of Calcium phosphate(CaP)scaffold by the addition of fiber has been well recognized,integrated mechanical,structural and functional considerations have been neglected in the design and fabrication of CaP scaffold *** emerging 3D printing provides a promising technique to construct CaP scaffold with precise size and elaborate ***,the most challenge is to extrude smoothly the CaP paste containing fibers for frequently-used extrusion-based 3D *** this study,frozen section and chemical dispersant(Pluronic F127,F127)were employed jointly to prepare non-aggregated polylactic-co-glycolic acid(PLGA)*** injectability of CaP pastes with well dispersed PLGA fibers was more than 90%when the content of PLGA fibers was no more than 3 wt%.Meanwhile rheological property of CAP pastes with well dispersed fibers showed shear thinning,which were both beneficial to extrude CaP paste with well dispersed fibers for 3D ***,these CaP scaffolds showed ductile fracture behavior due to the pullout and bridging effect of PLGA *** cell proliferation and alkaline phosphatase(ALP)activity indicated that 3D printed CAP scaffold containing PLGA fibers possesses excellent biocompatibility and facilitate osteogenic differentiation ***,it was feasible to print CaP pastes with well dispersed PLGA fibers to construct toughening CaP scaffolds with the higher shape fidelity and complex structures,which had significant clinical potentials in osteoanagenesis due to their higher toughness and excellent biocompatibility.

关键词： CaP scaffold extrusion-based 3D printing PLGA fiber toughening biocompatibility

山茶油干预的阿尔茨海默病小鼠尿液代谢组学分析

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食品科学 2024年第8期45卷 114-121页

作者：王瑞锋周宁陈龙刘通郭彭莉张冰贤张振凯曾梦楠熊维政郑晓珂冯卫生河南中医药大学药学院河南郑州450046 河南绿达山茶油股份有限公司河南信阳465550 河南省中药开发工程技术研究中心河南郑州450046

目的:基于超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography-quadrupole time-of-flightmassspectrometry,UPLC-Q/TOF-MS)技术,利用代谢组学方法探讨山茶油干预的阿尔茨海默病(Alzheimer’s disease,AD)小鼠尿液的代谢组学变化。方法:将小鼠随机分为正常对照组、模型组、阳性药组、山茶油组,除对照组外其余各组小鼠均由β-淀粉样蛋白25-35肽诱导AD,并取尿液样本。尿液沉淀蛋白后进行UPLC-Q/TOF-MS分析,获得小鼠尿液代谢物数据,使用SIMCA-P14.1软件进行主成分分析(principalcomponent analysis,PCA)和正交偏最小二乘判别分析,使用HMBD、MassBank、MetaboAnalyst、京都基因与基因组百科全书在线数据库对生物标志物进行鉴定、通路富集分析和代谢网络的构建。结果:在PCA得分图中,山茶油组样本点远离模型组样本点区域,更接近正常对照组样本点区域。鉴定得到48个生物标志物,主要涉及的通路有牛磺酸和次牛磺酸代谢,精氨酸和脯氨酸代谢,半胱氨酸和蛋氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢等。结论:山茶油通过调节能量代谢、氨基酸代谢等改善了AD小鼠体内代谢,为山茶油干预AD的进一步研究提供参考。

关键词：代谢组学山茶油小鼠尿液阿尔茨海默病

Studying the nature of ascending-descending-floating-sinking of Chinese medicines based on gonadotropin-releasing hormone

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Journal of Traditional Chinese Medicine 2022年第4期42卷 546-555页

作者： FU Yang YUAN Peipei ZENG Mengnan HOU Ying GAO Liyuan WEI Yaxin zheng xiaoke FENG Weisheng Department of Pharmacy Henan University of Chinese MedicineThe Engineering and Technology Center for Chinese Medicine Development of Henan ProvinceCo-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education MinistryZhengzhou 450046China

OBJECTIVE:To explore the nature of ascendingdescending-floating-sinking of Traditional Chinese Medicine on normal rats using gonadotropin-releasing hormone(GnRh).METHODS:Normal male Sprague-Dawley rats were orally administered six floating Chinese medicines and seven sinking Chinese medicines for 14 ***,GnRh and relevant indicators were ***,the different effects of floating and sinking drugs on the body were *** verify the effects of floating and sinking drugs on the body,normal rats were orally administered Mahuang(Herba Ephedra Sinica)and Tinglizi(Semen Lepidii Apetali)for 14 ***,GnRh antagonists were *** observed the changes of relevant indicators and clarified the correlation between GnRh and the laws of floating and ***:Floating Chinese medicines significantly increased the testicular coefficients;GnRh level in the serum;the protein level of GnRh receptor(GnRhR)in the testis;and the mRNA levels of androgen receptor(AR),small C-terminal domain phosphatase(SCP)3,SCP2,SYCE1,SMC1 B,SMC3,and Rec8(P<0.01 or<0.05).Sinking Chinese medicines did not react similarly,while GnRh antagonists blocked the regulatory effect of Mahuang(Herba Ephedra Sinica)but did not affect Tinglizi(Semen Lepidii Apetali).CONCLUSIONS:GnRh may be closely related to the nature of ascending-descending-floating-sinking of Chinese *** Chinese medicines may promote their medicinal properties by regulating Gn RH level;however,sinking Chinese medicines did not affect GnRh level.

关键词： gonadotropin-releasing hormone receptors,LHRH Mahuang(Herba Ephedra Sinica) Tinglizi(Semen Lepidii Apetali) testis ascending-descending-floating-sinking

A new megastigmane from fresh roots of Rehmannia glutinosa

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Acta Pharmaceutica Sinica B 2013年第5期3卷 333-336页

作者： Weisheng Feng Yanyan Lv xiaoke zheng Yanli Zhang Yangang Cao Yuanyuan Pei School of Pharmacy Henan University of Traditional Chinese MedicineZhengzhou 450046China

A new megastigmane,rehmamegastigmane(1),together with eighteen known compounds lariciresinol(2),lariciresinol-4′-O-β-D-glucopyranoside(3),hierochin D(4),yemuoside YM1(5),darendoside B(6),decaffeoylacteoside(7),jionoside B1(8),catalpol(9),ajugol(10),6-O-vanilloylajugol(11),6-O-E-feruloylajugol(12),rehmapicroside(13),rehmapicrogenin(14),3-methoxy-2,6,6-trimethylcyclohexane-1-enecarboxylic acid(15),vanillic acid(16),hydroferulic acid(17),threo-1-(4-hydroxy-3-methoxyphenyl)-1,2,3-propanetriol(18),p-hydroxyphenylethyl alcohol(19)was isolated from the fresh roots of Rehmannia *** 2–6 and 16–18 were isolated from this plant for the first time.

关键词： Scrophulariaceae Rehmannia glutinosa Chemical constituents Iridoids