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Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal sIgA secretions and gut microbial populations in weaned piglets

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Journal of Integrative Agriculture 2017年第9期16卷 2029-2037页

作者： ZHU Cui WANG Li WEI Shao-yong CHEN Zhuang MA Xian-yong zheng Chun-tian JIANG Zong-yong Key Laboratory of Animal Nutrition and Feed (South China) Ministry of Agriculture/Institute of Animal ScienceGuangdong Academy of Agricultural Sciences Agro-biological Gene Research Center Guangdong Academy of Agricultural Sciences

This study was conducted to determine the effect of different forms of yeasts Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal secretory immunoglobulin A（s Ig A） secretions and gut microbial populations in weaned piglets. A total of 96 piglets weaned at 14 d of age were randomly allotted to 4 dietary treatments：（1） basal diet without yeast（Control）;（2） basal diet supplemented with 3.00 g kg–1 live yeast（LY）;（3） basal diet supplemented with 2.66 g kg–1 heat-killed whole yeast（HKY）; and（4） basal diet supplemented with 3.00 g kg–1 superfine yeast powders（SFY）. Each treatment had 4 replicates（pens）, with 6 piglets per replicate. The experiment lasted for 3 wk. At d 7 and 21 of the experiment, the samples of serum, mucosa and mesenteric lymph node（MLN） from jejunum, and digesta from the ileum and cecum were collected for determinations. Compared with the Control, dietary SFY supplementation increased serum superoxide dismutase（SOD） activity and lysozyme levels at d 7, and jejunum mucosal s Ig A secretions at d 21 of the experiment（P〈0.05）. Dietary LY supplementation increased serum SOD activity and jejunum mucosal s Ig A secretions, but decreased serum malondialdehyde（MDA） concentration at d 7 and 21（P〈0.05）. Piglets fed diets supplemented with LY and SFY had lower p H values and decreased numbers of Escherichia coli in the ileum and cecum contents at d 21 compared with the Control（P〈0.05）. Moreover, the ratio of Lactobacilli to E. coli in the ileum and cecum contents was increased by dietary LY and SFY supplementations（P〈0.05）. Collectively, different forms of yeasts, especially LY and SFY, may modulate body antioxidant capacity and enhance the intestinal immunity by regulation of secretions of mucosal s Ig A and reduction of pathogenic bacteria colonization, thus improving intestinal health of weaned piglets.

关键词： Saccharomyces cerevisiae antioxidant capacity sIgA intestinal health gut microbiota weaned piglets

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OsHemA gene, encoding glutamyl-tRNA reductase(GluTR) is essential for chlorophyll biosynthesis in rice(Oryza sativa)

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Journal of Integrative Agriculture 2020年第3期19卷 612-623页

作者： ZENG Zhao-qiong LIN tian-zi ZHAO Jie-yu zheng tian-hui XU Le-feng WANG Yi-hua LIU Ling-long JIANG Ling CHEN Sai-hua WAN Jian-min State Key Laboratory for Crop Genetics&Germplasm Enhancement/Jiangsu Provincial Center of Plant Gene Engineering/College of Agriculture Nanjing Agricultural UniversityNanjing 210095P.R.China Nanchong Academy of Agricultural Sciences Nanchong 637000P.R.China Key Laboratory of Plant Functional Genomics Ministry of Education/CoInnovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009P.R.China

Chlorophyll(Chl) biosynthesis is essential for photosynthesis and plant ***-tRNA reductase(GluTR) catalyzes glutamyl-tRNA into glutamate-1-semialdehyde(GSA) and initiates the chlorophyll *** though the main role of GluTR has been established,the effects caused by natural variations in its corresponding gene remain largely ***,we characterized a spontaneous mutant in paddy field with Chl biosynthesis deficiency,designated as *** intact thylakoid lamellar structure,the cbd1 plant showed light green leaves and reduced Chl and carotenoids(Cars) content significantly compared to the wild *** map-based gene cloning,the mutation was restricted within a 57-kb region on chromosome 10,in which an mPingA miniature inverted-repeat transposable element(MITE) inserted in the promoter region of OsHemA *** leaf color and the pigment contents in cbd1 were recovered in a complementation test,confirming OsHemA was responsible for the mutant *** was uniquely predicted to encode GluTR and its expression level was dramatically repressed in *** transformation in protoplasts demonstrated that GluTR localized in chloroplasts and a signal peptide exists in its N-terminus.A majority of Chl biosynthesis genes,except for POR and CHLG,were down-regulated synchronously by the repression of OsHemA,suggesting that an attenuation occurred in the Chl biosynthesis ***,we found major agronomic traits involved in rice yield were statistically unaffected,except for the number of full grains per panicle was increased in ***,OsHemA plays an essential role in Chl biosynthesis in rice and its weak allele can adjust leaf color and Chls content without compromise to rice yield.

关键词： OsHemA GluTR chlorophyll biosynthesis

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A series of high-nuclear planar equilateral triangle-shaped {Ln_(6)(μ_(3)-OH)_(6)} cluster encapsulated polyoxoniobates with frequency dependent magnetic property

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Chinese Chemical Letters 2023年第7期34卷 360-363页

作者： Zeng-Kui Zhu Ya-Yun Lin Rong-Da Lai Xin-Xiong Li Yan-Qiong Sun Shou-tian zheng State Key Laboratory of Photocatalysis on Energy and Environment College of ChemistryFuzhou UniversityFuzhou 350108China

The integration of lanthanide(Ln)ions and polyoxoniobates(PONbs)is challenging,and the know Ln-substituted PONbs are still *** work introduces high-nuclear iso-Ln-oxo clusters into th PONb *** first series of high-nuclear Ln-oxo clusters encapsulated heterometallic polyoxonio bates H_9[Na(H_(2)O)_(4)][Cu(en)_(2)]_(10){Ln_6(μ_(3)-OH)_6(Si Nb_(18)O_(54))_(3)}·18H_(2)O(1-Ln,en=ethylenediamine,Ln=Dy,Gd Tb,Ho,Er,Tm,Yb,Lu)based on flower-like{Ln_(6)(μ_(3)-OH)_(6)(Si Nb_(18)O_(54))_(3)}({Ln_6Si_(3)Nb_(54)})clusters hav been successfully synthesized via one-pot hydrothermal synthesis *** flower-like polyoxoanio{Ln_6Si_(3)Nb_(54)}is consisted of three heteropolyoxoniobate{Si Nb_(18)O_(54)}clusters and one unique planar equ lateral triangle-shaped{Ln_6(μ_(3)-OH)_6}cluster,which presents the highest nuclear iso-Ln-oxo cluster i PONb ***{Ln_6(μ_(3)-OH)_6}cluster,each pair ofμ_(3)-OH groups link three Dy^(3+)ions to form a sma approximate equilateral triangle-shaped{Dy_(3)(OH)_(2)}***,the three{Dy_(3)(OH)_(2)}cluster comprise a bigger approximate equilateral triangle-shaped{Dy_6(μ_(3)-OH)_6}*** reported hexanu clear{Ln_6}cluster skeletons are mostly octahedral,however,such equilateral triangle-shaped skeleton o the hexanuclear Ln-oxo cluster is first *** 1-Dy exhibits good water vapor adsorption capacit and ferromagnetic properties.

关键词： Heteropolyoxoniobate Hexanuclear iso-Ln-oxo cluster Copper complexes Water vapor adsorption Ferromagnetism

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Seepage characteristics of parallel flow and radiation flow in rock fractures

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Yantu Lixue/Rock and Soil Mechanics 2020年 41卷 11-18页

作者： Wang, Zhe-Chao zheng, tian Yang, Jin-Jin Liu, Jie Li, Wei Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines Northeastern University Shenyang 110819 China

In underground engineering, parallel flow and radial flow are two kinds of groundwater flow configurations that mainly affects the seepage characteristics. In this paper, the models based on two flow configurations were established respectively to calculate roughness using different types of selected lines. Through theoretical analysis and numerical simulation, the critical Reynolds number, equivalent hydraulic aperture, non-Darcy coefficient and microscopic flow characteristics of single fracture in different flow configurations were studied. It is found that the relation between macroscopic pressure gradient and flow rate in the fracture meets the Forchheimer equation under two flow configurations. Under two different flow configurations, as the fracture roughness increases, the critical Reynolds number and the equivalent hydraulic opening decrease, while the non-Darcy coefficient increases. Compared the parallel flow with that of the radiant flow under the same condition, it shows that the critical Reynolds number and the equivalent hydraulic aperture of the radial flow configuration are much lower, and the roughness has a much greater influence on the non-Darcy coefficient. © 2020, Science Press. All right reserved.

关键词： Radial flow

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Computer-assisted minimally invasive spine surgery for resection of ossification of the ligamentum flavum in the thoracic spine

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Chinese Medical Journal 2014年第11期127卷 2043-2047页

作者： Yuan Qiang zheng Shan tian Wei Department of Spine Surgery Beijing Jishuitan Hospital the Fourth Clinical Medical College of Peking University Beijing 100035China

Background Ossification of the ligamentum flavum （OLF） has been widely recognized as one of the main causes of thoracic spinal canal stenosis and thoracic *** is the only effective strategy for treating thoracic myelopathy caused by *** purpose of this study was to describe the clinical outcomes of computer-assisted minimally invasive spine surgery （CAMISS） for posterior decompression in patients with thoracic myelopathy caused by *** In all cases,the surgical procedure was performed with the assistance of an intraoperative three-dimensional navigation *** of the spinal cord was performed with a high-speed drill; the supraspinal ligaments and spinous process were partially *** outcomes were evaluated by a modified Japanese Orthopedic Association （JOA） scoring system and recovery *** The mean duration of follow-up for the 14 cases was 3.9 *** patients experienced neurological recovery,the mean JOA score improving from 6.1 points preoperatively to 8.6 points at final follow-up and the mean rate of recovery being 52.7% （excellent in two cases,good in eight,fair in three,and unchanged in one）.Conclusion CAMISS is a safe and effective procedure for resection of the OLF in the thoracic spine.

关键词： computer-assisted minimally invasive spine surgery ligamentum flavum, ossification thoracic spinal canal stenosis decompression

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Graphitic carbons:preparation,characterization,and application on K-ion batteries

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Rare Metals 2024年第9期43卷 4056-4075页

作者： Kang-Zhe Cao Jia-Hui Ma Yu-Lian Dong Yu Duan Run-tian zheng Dharani Bundhooa Hui-Qiao Liu Yong Lei College of Chemistry and Chemical Engineering Xinyang Normal UniversityXinyang 464000China Xinyang Key Laboratory of Low-Carbon Energy Materials Xinyang 464000China Fachgebiet Angewandte Nanophysik Institut fur Physik&IMN MacroNanoTechnische Universitat Ilmenau98693 IlmenauGermany Laboratory of Inorganic Materials Chemistry(CMI) University of NamurB-5000 NamurBelgium

K-ion batteries(KIBs)have drawn much attention due to the abundant potassium reserves and wide accessibility as well as high energy density,which can be designed for large-scale energy storage *** the most promising anode materials for KIBs,graphitic carbons,especially those with an intermediate structure between the crystalline graphite and amorphous carbons become a hot research focus because of the improved rate capability and enhanced diffusion-controlled capacity at low voltage ***,we first review the structures of graphitic carbons in the view of graphitic domains and the structure changes in their K-ion intercalation ***,we summarize the preparation mechanisms and characterizations of graphitic carbons and the influence factors in their degree of ***,we illustrate the strategies to optimize their K-ion storage properties from four aspects,namely graphitic domain design,microstructure engineering,electrochemical active component regulation,and defect ***,we propose the issues that urgently need to be solved in graphitic carbons and the possible *** hope that this view could offer some inspiration for the further designing and optimizing of graphitic carbons for practical KIBs.

关键词： Graphitic carbon K-ion battery Anode Graphite Structure design

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Revealing the Structure-Property Difference of Natural Rubber Prepared by Different Methods: Protein and Gel Content are Key Factors

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Chinese Journal of Polymer Science 2024年第4期42卷 457-467页

作者： Shao-Qi Huang Jun-Qi Zhang Yong Zhu Ling-Min Kong Lu-Sheng Liao Fu-Quan Zhang zheng-tian Xie Jin-Rong Wu State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu610065China Hainan Provincial Key Laboratory of Natural Rubber Processing Agricultural Products Processing Research Institute of Chinese Academy of Tropical Agricultural SciencesZhanjiang524001China

Natural rubber (NR) is widely used in various fields including aerospace, military industry and transportation due to its superior elasticity and comprehensive mechanical properties. Nonetheless, the commercial NR prepared by different methods usually exhibits different mechanical properties, primarily due to variations in processing conditions during the conversion from latex to bulk rubber material. Consequently, this poses challenges in scientific research and industrial production of NR. In order to assess the properties of various commercially available NR and identify key structural and compositional components, this study systematically compares and analyzes four representative NR raw materials: air dried sheet (ADS), ribbed smoked sheets (RSS), constant viscidity NR (CV), and whole field latex rubber (WF). The investigation focuses on evaluating their static mechanical behavior, SIC behavior, wear resistance, and fatigue resistance. The findings indicate that protein and gel content exhibit a crucial influence on the NR properties. These constituents contribute to the formation of a high-crosslinking density region, generating a heterogeneous network structure within the rubber. This structure amplifies strains during deformation, leading to earlier and stronger strain-induced crystallization (SIC). Among the four commercial NR brands, RSS demonstrates superior overall mechanical and dynamic properties owing to its high protein and gel content. This study serves as a valuable reference for comprehending the differences in properties among various commercial NR, thereby offering guidance for the actual processing and selection of NR.

关键词： Natural rubber Processingmethod Protein Gel content Property

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Recycle Adsorption of Cu2＋ on Amine-functionalized Mesoporous Silica Monolithic

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Chemical Research in Chinese Universities 2013年第4期29卷 793-797页

作者： ZHAO Da-zhou JING Shu-bo XU Jia-ning YANG Hong zheng Wei SONG tian-you ZHANG Ping State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

3-Aminopropyltriethoxysilane functionalized worm-like mesoporous silica monolithic（WMSM-NH2） was prepared and used as a new regenerable adsorbent for the removal of Cu2＋ ions. The analysis results show that the WMSM-NH2 monolithic had a high efficiency value of 99.3% for Cu2＋ remediation when the Cu2＋ solution was at an initial concentration of 10.32 mg/L. The regeneration study of the WMSM-NH2 monolithic presented that the adsorp- tion efficiency of 89.0% was remained and a mass of 92.0% was left after seven adsorption-desorption cycles were executed. The monolithic material with high resistance to the acid and good mechanical stability can facilitate the operations of adsorption and regeneration of the adsorbent.

关键词： Amine-functionalized mesoporous silica monolithic(WMSM-NH2) Cu2+ removal Multiple cycle Adsorp- tion Easy operation

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Catastrophe theory-based risk evaluation model for water and mud inrush and its application in karst tunnels

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Journal of Central South University 2020年第5期27卷 1587-1598页

作者： ZHU Jian-qun LI tian-zheng School of Civil Engineering and Architecture Changzhou Institute of TechnologyChangzhou 213002China School of Civil Engineering Central South UniversityChangsha 410075China

This paper presents a risk evaluation model of water and mud inrush for tunnel excavation in karst *** factors affecting the probabilities of water and mud inrush in karst tunnels are investigated to define the dangerousness of this geological *** losses that are caused by water and mud inrush are taken into consideration to account for its *** a risk evaluation model based on the dangerousness-harmfulness evaluation indicator system is constructed,which is more convincing in comparison with the traditional *** catastrophe theory is used to evaluate the risk level of water and mud inrush and it has great advantage in handling problems involving discontinuous catastrophe *** validate the proposed approach,the Qiyueshan tunnel of Yichang-Wanzhou Railway is taken as an example in which four target segments are evaluated using the risk evaluation ***,the evaluation results are compared with the excavation data,which shows that the risk levels predicted by the proposed approach are in good agreements with that observed in *** conclusion,the catastrophe theory-based risk evaluation model is an efficient and effective approach for water and mud inrush in karst tunnels.

关键词： risk evaluation model water and mud inrush catastrophe theory karst area tunneling

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Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles

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Agricultural Sciences in China 2011年第1期10卷 120-125页

作者： JIANG Zong-yong ZHOU Gui-lian LIN Ying-cai JIANG Shou-qun zheng Chun-tian HAN Li-ming CHEN Fang Key Laboratory of Animal Nutrition and Feed Science （South China） Ministry of Agriculture/Institute of Animal Science Guangdong Academy of Agricultural Science Guangzhou 510640 P.R.China

A synthetic isoflavone （ISO-S） or genistein was added in culture medium at different concentrations （0, 10, 20, 30, 40, and 80 *** L^-1） to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells. After 48 h incubation, the suspension was cryopreserved for the determination of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px） activities, and malondialdehyde （MDA） content. The mRNA levels of SOD, CAT, and GSH-Px gene in cells were detected with Taqman fluorescent probe method. The results showed that the content of MDA and the activities and the mRNA levels of SOD of porcine skeletal muscle satellite cells were influenced by supplemented soybean isoflavone （P〈0.05） when adding 10-80 μmol L^-1 ISO-S or genistein in the medium. The MDA contents, SOD and CAT activities and their mRNA expression levels of porcine skeletal muscle cells responded quadratically （P〈 0.05） as the level of ISO-S or genistein increased. Pre-incubation of porcine skeletal muscle satellite cells with ISO-S or genistein at 10-40 pmol L-1 elevated the activities and the mRNA expression levels of SOD and CAT in cells concurrently and decreased the cellular content of MDA （P〈 0.05）. The results indicated that pre-incubation of ISO-S or genistein at 10- 40μmol L^-1 could improve the antioxidative capacity of porcine skeletal muscle satellite cells.

关键词： soybean isoflavone antioxidative capacity superoxide dismutase catalase skeletal muscle satellite cells

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