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基于革兰染色涂片的需氧菌性阴道炎诊断标准在阴道微生态自动化检测中的应用研究

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中国实用妇科与产科杂志 2024年第5期40卷 556-560页

作者：范琳媛白会会刘朝晖曹正宗晓楠张展李婷首都医科大学附属北京妇产医院/北京妇幼保健院妇科北京100026 首都医科大学附属北京妇产医院/北京妇幼保健院检验科北京100026

目的探讨基于革兰染色涂片结合临床特征的需氧菌性阴道炎(aerobic vaginitis,AV)联合诊断标准整合到阴道微生态自动检测系统中的准确性。方法基于AV联合诊断标准,将白细胞数、基底旁细胞占比、乳杆菌分级、背景菌落和临床特征(阴道pH值... 详细信息

目的探讨基于革兰染色涂片结合临床特征的需氧菌性阴道炎(aerobic vaginitis,AV)联合诊断标准整合到阴道微生态自动检测系统中的准确性。方法基于AV联合诊断标准,将白细胞数、基底旁细胞占比、乳杆菌分级、背景菌落和临床特征(阴道pH值、阴道黏膜充血和黄色分泌物)5项评价指标整合到仕达思Comet-60au高倍镜检分析系统中。研究所用样品来自2023年1月至8月首都医科大学附属北京妇产医院门诊进行妇科阴道微生态评价系统检测的样本,共采用1184例样品用于AV自动化诊断的模型开发,进行机器学习,每项指标0~2分,≥4分时诊断为AV。以临床金标准检测结果为最终诊断结果,517例用于模型的验证以评价自动化诊断的准确度。结果在模型建立阶段,乳杆菌分级指标总体一致率73.8%,白细胞数一致率68.6%,背景菌群一致率32.7%,基底旁细胞占比一致率57.0%。AV阴性一致率98.3%,最终两种诊断方法总体一致率96.5%,在AV检出率中差异无统计学意义(P=1.000)。进一步的临床验证阶段,两种检测方法总体一致率87.02%,各项指标总体一致率由高到低依次为基底旁细胞占比(94.6%)、背景菌群(70.8%)、乳杆菌分级(69.6%)、白细胞数(59.6%),各项指标正常的一致率均>95%。结论基于革兰染色涂片结合临床特征的AV联合诊断标准开发的AV自动检测具有可行性,AV自动化检测与临床金标准检测总体一致性较高,但灵敏度有待提高,需要在临床应用中进行更大样本量模型的优化与验证。

关键词：需氧菌性阴道炎革兰染色自动化诊断阴道微生态

Formation mechanism of calcium hexaluminate

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International Journal of Minerals,Metallurgy and Materials 2016年第10期23卷 1225-1230页

作者： Jun-hong Chen Hai-yang Chen Ming-wei Yan zheng cao Wen-jun Mi School of Materials Science and Engineering University of Science and Technology Beijing

To investigate the formation mechanism of calcium hexaluminate（CaAl_（12）O_（19）, CA_6）, the analytically pure alumina and calcia used as raw materials were mixed in cao/Al_2O_3 ratio of 12.57：137.43 by mass. The raw materials were ball-milled and shaped into green specimens, and fired at 1300-1600°C. Then, the phase composition and microstructure evolution of the fired specimen were studied, and a first principle calculation was performed. The results show that in the reaction system of cao and Al_2O_3, a small amount of CA_6 forms at 1300°C, and greater amounts are formed at 1400°C and higher temperatures. The reaction is as follows： cao ·2Al_2O_3（CA_2）＋ 4Al_2O_3 → CA_6. The diffusions of Ca^（2＋） in CA_2 towards Al_2O_3 and Al^（3＋） in Al_2O_3 towards CA_2 change the structures in different degrees of difficulty. Compared with the difficulty of structural change and the corresponding lattice energy change, it is deduced that the main formation mechanism is the diffusion of Ca^（2＋） in CA_2 towards Al_2O_3.

关键词： calcium hexaluminate microstructural evolution first principle formation mechanisms

Characteristics of Shale Reservoir and Sweet Spot Identification of the Lower Cambrian Niutitang Formation in Northwestern Hunan Province, China

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Acta Geologica Sinica(English Edition) 2019年第3期93卷 573-587页

作者： QIN Mingyang cao zheng GUO Jianhua HUANG Yanran SUN Lianpu DONG Li Central South University Press Changsha 410083China School of Geosciences and Info-Physics Engineering Central South UniversityChangsha 410083China The Survey Academy of Coal Geology in Hunan Changsha 410014China School of Geosciences China University of PetroleumQingdao 266580China School of Petroleum Engineering Chongqing University of Science and TechnologyChongqing 401331China Hunan Provincial Key Laboratory of Shale Gas Resource Utilization Hunan University of Science and TechnologyXiangtan411201China Sino Geotech(Beijing)Science&Technology Co. Ltd.Beijing 100020China Exploration&Production Research Institute SINOPEC Beijing 100083China Shandong Provincial Key Laboratory of Depositional Mineralization&Sedimentary Mineral Shandong University of Science and TechnologyQingdao 266590China

The accumulation and productivity of shale gas are mainly controlled by the characteristics of shale reservoirs;study of these characteristics forms the basis for the shale gas exploitation of the Lower Cambrian Niutitang Formation(Fm),Southern *** this study,core observation and lithology study were conducted along with X-ray diffraction(XRD)and electronic scanning microscopy(SEM)examinations and liquid nitrogen(N2)adsorption/desorption and CH4 isothermal adsorption experiments for several exploration wells in northwestern Hunan Province,*** results show that one or two intervals with high-quality source rocks(TOC>2 wt%)were deposited in the deep-shelf *** source rocks,which were mainly composed of carbonaceous shales and siliceous shales,had high quartz contents(>40 wt%)and low clay mineral(50 nm in diameter).The shale reservoir has strong adsorption capacity for *** Langmuir volume(VL)varied from 1.63 to 7.39 cm^3/g,with an average of 3.95 cm^3/*** characteristics of shale reservoir are controlled by several factors:(1)A deep muddy continental shelf is the most favorable environment for the development of shale reservoirs,which is controlled by the development of basic materials.(2)The storage capacity of the shale reservoir is positively related to the TOC contents and plastic minerals and negatively related to cement minerals.(3)High maturity or overmaturity leads to the growth of organic pores and microfractures,thereby improving the reservoir storage *** can be deduced that the high percentage o

关键词： Northwestern Hunan Province Niutitang Formation reservoir characteristics control factors sweet spots identification

基于超声波流量计的间接测量法在输液泵流量校准中的应用

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医疗装备 2024年第15期37卷 6-9页

作者：曹峥李菲吕冉李智袁文斌曹瑞亭吴迪然北京市东城区计量检测所北京100061 中纺标检验认证股份有限公司北京100025 基恩士(中国)有限公司北京100073

目的比较基于超声波流量计的间接测量法和采用输液设备分析仪的直接测量法在输液泵流量校准中的应用效果。方法按照JJF 1259-2018《医用注射泵和输液泵校准规范》,分别采用直接测量法(采用输液设备分析仪)及间接测量法(采用超声波流量计... 详细信息

目的比较基于超声波流量计的间接测量法和采用输液设备分析仪的直接测量法在输液泵流量校准中的应用效果。方法按照JJF 1259-2018《医用注射泵和输液泵校准规范》,分别采用直接测量法(采用输液设备分析仪)及间接测量法(采用超声波流量计)对被测输液泵进行流量校准,比较2种方法的测量相对误差和测量时间。结果间接测量法的测量时间显著少于直接测量法。但间接测量法在低流量校准区间(10~200 ml/h)的相对误差过大。对可能导致间接测量法相对误差较大的原因进行分析并改进后,间接测量法在各校准点的相对误差均降至可接受范围。结论输液泵流量校准中,相较于采用输液设备分析仪的直接测量法,基于超声波流量计的间接测量法可在保证校准准确性的同时有效缩短测量时间,为输液泵使用量大的医疗机构提供一种更有效、便捷的期间核查方式。

关键词：超声波流量计输液泵流量校准期间核查

Real-Time Hybrid Flow Shop Scheduling Approach in Smart Manufacturing Environment

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Complex System Modeling and Simulation 2021年第4期1卷 335-350页

作者： Xiuli Wu zheng cao Shaomin Wu Department of Logistics the School of Mechanical EngineeringUniversity of Science and Technology BeijingBeijing 100083China Kent Business School University of KentKentCT27FSUK.

Smart manufacturing in the“Industry 4.0”strategy promotes the deep integration of manufacturing and information technologies,which makes the manufacturing system a ubiquitous ***,the real-time scheduling of such a manufacturing system is a challenge faced by many decision *** deal with this challenge,this study focuses on the real-time hybrid flow shop scheduling problem(HFSP).First,the characteristic of the hybrid flow shop in a smart manufacturing environment is analyzed,and its scheduling problem is ***,a real-time scheduling approach for the HFSP is *** core module is to employ gene expression programming to construct a new and efficient scheduling rule according to the real-time status in the hybrid flow *** the scheduling rule,the priorities of the waiting job are calculated,and the job with the highest priority will be scheduled at this decision time point.A group of experiments are performed to prove the performance of the proposed *** numerical experiments show that the real-time scheduling approach outperforms other single-scheduling rules and the back-propagation neural network method in optimizing most objectives for different size ***,the contribution of this study is the proposal of a real-time scheduling approach,which is an effective approach for real-time hybrid flow shop scheduling in a smart manufacturing environment.

关键词： smart manufacturing real-time scheduling hybrid flow shop scheduling problem gene expression programming

Rno_circ_0005139 regulates apoptosis by targeting Wnt5a in rat anorectal malformations

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World Journal of Gastroenterology 2020年第29期26卷 4272-4287页

作者： Dan Liu Yuan Qu zheng-Nong cao Hui-Min Jia Department of Pediatric Surgery Shengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina

BACKGROUND The molecular mechanisms underlying anorectal malformations(ARM)are not fully *** RNAs(circRNAs)are new born non-coding RNAs,and their role in ARM is *** assumed that rno_circ_0005139 influences apoptosis and proliferation by acting as a miR-324-3p sponge,and downregulating Wnt5a in *** To identify the differential expression of circRNAs and mRNAs in a rat ARM *** Sixty-six pregnant Wistar rats were randomly divided into two groups:ARM group(2-imidazolidinethione-induced)and control *** were harvested by cesarean delivery,and anorectal tissue was taken on embryonic days 16(E16),17(E17),19(E19),and 21(E21).RNA sequencing and gene microarray analysis was used to identify differentially expressed circRNAs and mRNAs in the ARM in a rat *** selected 6 circRNAs and 3 mRNAs in the Wnt signal pathway from the result of the RNA sequencing and gene microarray analysis,and quantitative reverse transcription polymerase chain reaction was performed to evaluate their tissue *** to bioinformatics prediction,rno_circ_0005139 acted as a miR-324-3p sponge to regulate the expression of *** chose rno_circ_0005139 and Wnt5a as the final *** tested the function of rno_circ_0005139 and the binding sites between rno_circ_0005139 and miR-324-3p,miR-324-3p and Wnt5a by luciferase ***-transfection of rno_circ_0005139 and miR-324-3p was to verify their functional *** We identified 38 upregulated and 42 downregulated circRNAs on E17(P2.0).We found that rno_circ_0006880 and rno_circ_0011386 were upregulated,whereas rno_circ_0000436,rno_circ_0005139,rno_circ_0009285,rno_circ_0014367,Wnt5a,Wnt10b,and Wnt2b were downregulated in ARM *** to bioinformatics prediction,rno_circ_0005139 acted as a miR-324-3p sponge to regulate the expression of *** chose rno_circ_0005139 a

关键词： Anorectal malformation Circular RNA MicroRNA Wnt5a Rno_circ_0005139

Plasma spray coating on interconnector toward promoted solid oxide fuel cells and solid oxide electrolysis cells

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Frontiers in Energy 2024年第3期18卷 390-398页

作者： Junwen cao Yun zheng Wenqiang ZHANG Bo YU Institute of Nuclear and New Energy Technology(INET) Collaborative Innovation Center of Advanced Nuclear Energy TechnologyTsinghua UniversityBeijing 100084China Department of Chemical Engineering University of WaterlooWaterlooOntario N2L 3G1Canada Institute of New Energy Materials and Engineering School of Materials Science and EngineeringFuzhou UniversityFuzhou 350108China

Interconnector is a critical component to construct solid oxide cells (SOCs) stack. Oxidation of metallic interconnectors and Cr poisoning caused by oxidation are important factors that lead to long-term performance degradation of SOCs. Coating on the interconnector surface is an important approach to inhibit the oxidation and Cr migration of the interconnector. Herein, (La0.75Sr0.25)0.95MnO3–δ (LSM) and Mn1.5Co1.5O4 (MCO) are used to fabricate the coatings of interconnector. Two advanced thermal spray technology, atmospheric plasma spraying (APS) and low-pressure plasma spray (LPPS), are adopted for the coating preparation. The electrochemical performance, rising and cooling cycle stability, and Cr diffusion inhibition performance of the coatings are tested and evaluated. The result indicates that MCO can generate more uniform and denser coatings than LSM. In addition, MCO coatings prepared by LPPS shows the best electrochemical performance, rising and cooling cycle stability, and Cr diffusion inhibition. The initial area specific resistance (ASR) is 0.0027 Ω·cm2 at 800 °C. After 4 cooling cycle tests, the ASR increases to 0.0032 Ω·cm2 but lower than other samples. Meanwhile, the relative intense of Cr at the interface of SUS430 with MCO coatings fabricated by LPPS is lower than that of MCO fabricated by APS after 4 rising and cooling cycle operations, showing more favorable Cr diffusion inhibition performance.

关键词： interconnector coating plasma spray electrochemical performance Cr diffusion inhibition solid oxide cells(SOCs)

北京地区周岁内婴幼儿维生素D营养状况调查分析

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标记免疫分析与临床 2024年第1期31卷 16-21,47页

作者：谢杰施陈怡菲王殊曹正翟燕红首都医科大学附属北京妇产医院/北京妇幼保健院检验科北京100026

目的对本地区2022年7月至2023年6月来我院健康查体的周岁内婴幼儿维生素D营养状况和差异进行比较分析。方法选取2022年7月至2023年6月来我院健康查体的周岁内,无急、慢性疾病的婴幼儿作为研究对象。取受试儿童末梢血,利用荧光免疫层... 详细信息

目的对本地区2022年7月至2023年6月来我院健康查体的周岁内婴幼儿维生素D营养状况和差异进行比较分析。方法选取2022年7月至2023年6月来我院健康查体的周岁内,无急、慢性疾病的婴幼儿作为研究对象。取受试儿童末梢血,利用荧光免疫层析法对其血液中的总维生素D和25-羟基维生素D_(3)进行检测,并对数据进行分析和比较。结果本地区周岁内婴幼儿整体维生素D营养状况良好,3/4婴幼儿维生素D为理想水平,维生素D缺乏者占比小于1.3%,且无严重缺乏者。随月龄增大,维生素D理想水平的婴幼儿比例减低,维生素D充足和缺乏的婴幼儿比例增加。整体婴幼儿和男、女婴幼儿在周岁内总维生素D和25-羟基维生素D_(3)的平均值均随着月龄增加而呈现小幅波动,且不同月龄的总维生素D、25-羟基维生素D_(3)在整体和女婴幼儿中均存在差异(P<0.001)。除3月龄外,各月龄男、女婴幼儿的总维生素D、25-羟基维生素D_(3)及其差值、比值均无差异。半岁前,男、女婴幼儿的总维生素D和25-羟基维生素D_(3)的平均值从2月龄开始每两个月进行翻转,半岁后,整体呈每月翻转。结论本地区周岁内婴幼儿总体维生素D营养状况良好,但仍需加强婴幼儿维生素D的合理补充。

关键词：婴幼儿月龄维生素D营养状况总维生素D 25-羟基维生素D3

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机动目标跟踪时滞问题分析

电子技术应用 2024年第7期50卷 1-6页

作者：李暐琪柳超曹政张彦敏薛伟哈尔滨工程大学烟台研究院山东烟台264001 海军航空大学山东烟台264001 海洋电磁探测与控制湖北省重点实验室湖北武汉430071 武汉第二船舶设计研究所湖北武汉430064

机动目标跟踪结果在时间轴上的滞后问题是当前机动目标跟踪领域的一大难点。产生时滞的情况很多,一般在跟踪初期和发生较大机动的时间段内尤为明显,常常会因此出现误差高峰。如果能有方法抑制或者消除时滞现象,将能显著提高跟踪效果。... 详细信息

机动目标跟踪结果在时间轴上的滞后问题是当前机动目标跟踪领域的一大难点。产生时滞的情况很多,一般在跟踪初期和发生较大机动的时间段内尤为明显,常常会因此出现误差高峰。如果能有方法抑制或者消除时滞现象,将能显著提高跟踪效果。从仿真实验的结果和现象入手,结合卡尔曼滤波理论、交互式多模型算法和现代神经网络模型对时滞问题进行剖析,根据跟踪各个阶段情况的变化,分析时滞产生的不同原因,并提出可能的解决方法,以期为提高机动目标跟踪效果提供参考。

关键词：机动目标跟踪时滞卡尔曼滤波交互式多模型神经网络