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Application of sustainable basil seed as an eco-friendly multifunctional additive for water-based drilling fluids

Petroleum Science 2021年第4期18卷 1163-1181页

作者： Xin Gao Han-Yi Zhong Xian-Bin Zhang An-Liang Chen zheng-Song qiu Wei-An Huang Key Laboratory of Unconventional Oil&Gas Development(China University of Petroleum(East China)) Ministry of EducationQingdao266580ShandongChina School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Drilling Fluid Technology Services of CNPC BoHai Drilling Engineering Company Limited Dagang300280TianjinChina

Basil seed,containing anionic heteropolysaccharides in its outer pericarp,swells as gelatinous hydrocolloid when soaked in *** this study,basil seed powder(BSP)was used as a multifunctional additive for water-based drilling *** chemical composition,water absorbency,rheological properties of aqueous suspension of BSP were *** effect of BSP on the rheological and filtration of bentonitebased drilling fluid before and after thermal aging was *** inhibition characteristics were evaluated by linear swelling,shale cuttings dispersion and shale immersion *** improvement by BSP was measured with extreme pressure lubricity *** results revealed that incorporation of BSP into bentonite suspension improved rheological and filtration properties effectively after thermal aging of 120℃.BSP exhibited superior inhibitive capacity to xanthan and synergistic effect with *** could reduce friction by forming hydration *** nanoscale three-dimensional network structures enable BSP to maintain high water retention and absorb strongly on bentonite and metal surface,contributing to enhanced rheology,filtration,inhibition and lubrication *** versatile characteristic of BSP,as well as biodegradation makes it a promising additive using in high performance water-based drilling fluid and a potential alternative to conventional synthetic polymers.

关键词： Basil seed Water absorbency Multifunction Water-based drilling fluid Three-dimensional network

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Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

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World Journal of Gastroenterology 2009年第30期15卷 3757-3766页

作者： Guang Yan Chen Huang Jun Cao Ke-Jian Huang Tao Jiang zheng-Jun qiu Department of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai 200080 China

AIM： To investigate RNA interference targeting signal transducer and activator of transcription-3 （STAT3） on invasion of human pancreatic cancer cells.METHODS： We constructed three plasmids of RNA interference targeting the STAT3 gene. After LV （lentivirus）-STAT3siRNA （STAT3 small interfering RNA） the vector was transfected into the human pancreatic cell line, SW1990 and cell proliferation was measured by the MTT assay. Flow cytometry was used to assess cell cycle. Vascular endothelial growth favor （VEGF） and matrix metalloproteinase-2 （MMP-2） mRNA and protein expression were examined by quantitative PCR and western blotting, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay.RESULTS： We successfully constructed the LVSTAT3siRNA lentivirus vector and proved that it can suppress expression of STAT3 gene in SW1990 cells. RNA interference of STAT3 by the LV-STAT3siRNA construct significantly inhibited the growth of SW1990 cells, in addition to significantly decreasing both VEGF and MMP-2 mRNA and protein expression. Moreover, suppression of STAT3 by LV-STAT3siRNA decreased the invasion ability of SW1990 cells.CONCLUSION： The STAT3 signaling pathway may provide a novel therapeutic target for the treatment of pancreatic cancer since it inhibits the invasion ability of pancreatic cancer cells.

关键词： Signal transducer and activator of transcription3 RNA interference Lentivirus vector Pancreatic cancercells Invasion

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Thermo-sensitive polymer nanospheres as a smart plugging agent for shale gas drilling operations

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Petroleum Science 2017年第1期14卷 116-125页

作者： Wei-Ji Wang zheng-Song qiu Han-Yi Zhong Wei-An Huang Wen-Hao Dai School of Petroleum Engineering China University of Petroleum Qingdao 266580 Shandong China

Emulsifier-free poly（methyl methacrylate-styrene） [P（MMA-St）] nanospheres with an average particle size of 100 nm were synthesized in an isopropyl alcoholwater medium by a solvothermal method. Then, through radical graft copolymerization of thermo-sensitive mono- mer N-isopropylacrylamide （NIPAm） and hydrophilic monomer acrylic acid （AA） onto the surface of P（MMA- St） nanospheres at 80 ℃, a series of thermo-sensitive polymer nanospheres, named SD-SEAL with different lower critical solution temperatures （LCST）, were prepared by adjusting the mole ratio of NIPAm to AA. The products were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, thermogravimetric analysis, particle size distribution, and specific surface area analysis. The temperature-sensitive behavior was studied by light transmittance tests, while the sealing performance was investigated by pressure transmission tests with Lungmachi Formation shales. The experimental results showed that the synthesized nanoparticles are sensitive to temperature and had apparent LCST values which increased with an increase in hydrophilic monomer AA. When the temperature was higher than its LCST value, SD- SEAL played a dual role of physical plugging and chemical inhibition, slowed down pressure transmission, and reduced shale permeability remarkably. The plugged layer of shale was changed to being hydrophobic, which greatly improved the shale stability

关键词： Nanoparticle plugging agent Polymermicrospheres Thermo-sensitive polymer Wellborestability - Shale gas - Drilling fluid

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Changes of Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Waterlogging-Tolerant and Waterlogging-Sensitive Maize Genotypes at Seedling Stage

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Agricultural Sciences in China 2010年第5期9卷 651-661页

作者： TANG Bin XU Shang-zhong ZOU Xi-ling zheng Yong-lian qiu Fa-zhan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China

To better understand the physiological and biochemical mechanisms of waterlogging tolerance, waterlogging effects on lipid peroxidation and the activity of antioxidative enzymes were investigated in leaves and roots of two maize genotypes, HZ32 （waterlogging-tolerant） and K12 （waterlogging-sensitive）. Potted maize plants were waterlogged at the second leaf stage under glasshouse conditions. Leaves and roots were harvested 1 d before and 2, 4, 6, 8 and 10 d after the start of waterlogging treatment. Through comparing the activities of superoxide dismutase （SOD）, ascorbate peroxidase （APX）, glutathione reductase （GR）, catalase （CAT） and guaiacol peroxidase （POD） between waterlogging-tolerant and waterloggingsensitive genotype, we deduced that CAT was the most important H2O2 scavenging enzyme in leaves, while APX seemed to play a key role in roots. POD, APX, GR and CAT activities in conjunction with SOD seem to play an essential protective role in the O2^- and H2O2 scavenging process. Lipid peroxidation was enhanced significantly only in K12 （P 〈 0.001） and there was no difference （P 〉 0.05） in HZ32 up to 6 d after waterlogging stress. These results indicated that oxidative stress may play an important role in waterlogging-stressed maize plants and that the greater protection of HZ32 leaves and roots from waterlogging-induced oxidative damage results, at least in part, through the maintenance of increased antioxidant enzyme activity.

关键词： antioxidative enzymes lipid peroxidation maize (Zea mays L.) seedling stage waterlogging stress

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Cascade Enzymatic Hydrolysis Coupling with Ultrafine Grinding Pretreatment for Sugarcane Bagasse Saccharification

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Chinese Journal of Chemical Physics 2015年第3期28卷 355-360,I0002页

作者： zheng-qiu Yuan Jin-xing Long Tie-jun Wang Yu-qin Li Qi Zhang Long-long Ma Key Laboratory of Renewable Energy Guangzhou Institute of Energy Conversion Chinese Academy of Science Guangzhou 510640 China University of Chinese Academy of Science Beijing 100049 China School of Chemical EngineerinE Xiangtan University Xiangtan 411105 China

The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse saccharification. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 ℃, pH=4.8 for 72 h （1200 r/min） with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.

关键词： Sugarcane bagasse Ultrafine grinding pretreatment Cascade enzymatic hydrolysis Reducing sugars Glucose

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Impact of nutrient losses from agricultural lands on nutrient stocks in Dianshan Lake in Shanghai, China

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Water Science and Engineering 2014年第4期7卷 373-383页

作者： Shou-bing WANG Xiao-xue MA zheng-qiu FAN Wei-qian ZHANG Xiao-yong QIAN Department of Environmental Science and Engineering Fudan University Shanghai Academy of Environmental Sciences

Abstract： The water quality of Dianshan Lake in Shanghai Municipality, China, is impacted by nutrient losses from agricultural lands around the lake. In this study, nine types of agricultural land use were monitored in 2010 and 2011, and a correlation analysis between nutrient losses from agricultural non-point sources （NPS） and nutrient stocks in the lake was conducted over monthly and seasonal time periods. The results indicate that the monthly average concentration of total nitrogen （TN） ranged from 1.41 to 7.34 mg/L in 2010 and from 1.52 to 5.90 mg/L in 2011, while the monthly average concentration of total phosphorous （TP） ranged from 0.11 to 0.26 mg/L in 2010 and from 0.13 to 0.30 mg/L in 2011. The annual loss of TN from agricultural NPS was 195.55 tons in 2010 and 208.40 tons in 2011. The cultivation of water oat made the largest contribution to the loss of TN. The annual loss of TP was 44.58 tons in 2010 and 48.12 tons in 2011, and multi-vegetable cultivation made the largest contribution to the loss of TP. The results of correlation analysis show that the monthly stocks of TN and TP in the lake have a positive correlation with the monthly losses of TN and TP from agricultural NPS. According to the seasonal data, the stocks of TN and TP in the lake both have a much stronger correlation with the losses of TN and TP from agricultural NPS in summer than in other seasons. Agricultural NPS pollution control should be the main focus for the water resource conservation in this area.

关键词： agricultural non-point source pollution nutrient losses nutrient stocks totalnitrogen (TN) total phosphorus (TP) Dianshan Lake

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Basic Study Long non-coding RNA TP73-AS1 promotes pancreatic cancer growth and metastasis through miRNA-128-3p/GOLM1 axis

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World Journal of Gastroenterology 2021年第17期27卷 1993-2014页

作者： Bin Wang Xing Sun Ke-Jian Huang Li-Sheng Zhou zheng-Jun qiu Department of General Surgery Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China

BACKGROUND Previous studies have suggested that long non-coding RNAs(lncRNA)TP73-AS1 is significantly upregulated in several ***,the biological role and clinical significance of TP73-AS1 in pancreatic cancer(PC)remain *** To investigate the role of TP73-AS1 in the growth and metastasis of *** The expression of lncRNA TP73-AS1,miR-128-3p,and GOLM1 in PC tissues and cells was detected by quantitative real-time polymerase chain *** bioinformatics prediction software ENCORI was used to predict the putative binding sites of *** regulatory roles of TP73-AS1 and miR-128-3p in cell proliferation,migration,and invasion abilities were verified by Cell Counting Kit-8,wound-healing,and transwell assays,as well as flow cytometry and Western blot *** interactions among TP73-AS1,miR-128-3p,and GOLM1 were explored by bioinformatics prediction,luciferase assay,and Western *** The expression of TP73-AS1 and miRNA-128-3p was dysregulated in PC tissues and *** TP73-AS1 expression was correlated with a poor ***73-AS1 silencing inhibited PC cell proliferation,migration,and invasion in vitro as well as suppressed tumor growth in ***,TP73-AS1 was validated to promote PC progression through GOLM1 upregulation by competitively binding to *** Our results demonstrated that TP73-AS1 promotes PC progression by regulating the miR-128-3p/GOLM1 axis,which might provide a potential treatment strategy for patients with PC.

关键词： Pancreatic cancer Long non-coding RNA TP73-AS1 miR-128-3p GOLM1

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Numerical investigation of natural convection characteristics of a heat pipe-cooled passive residual heat removal system for molten salt reactors

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Nuclear Science and Techniques 2020年第7期31卷 25-33页

作者： Cheng-Long Wang Hao Qin Da-Lin Zhang Wen-Xi Tian Guang-Hui Su Sui-zheng qiu Department of Nuclear Science and Technology Xi’an Jiaotong UniversityXi’an 710049China Shaanxi Key Laboratory of Advanced Nuclear Energy and Technology Xi’an Jiaotong UniversityXi’an 710049China State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong UniversityXi’an 710049China

The limited availability of studies on the natural convection heat transfer characteristics of fluoride salt has hindered progress in the design of passive residual heat removal systems(PRHRS)for molten salt *** paper presents results from a numerical investigation of natural convection heat transfer characteristics of fluoride salt and heat pipes in the drain tank of a *** results are compared with experimental data,demonstrating the accuracy of the calculation *** distribution of fluoride salt and heat transfer characteristics are obtained and *** radial temperature of liquid fluoride salt in the drain tank shows a uniform distribution,while temperatures increase with increase in axial height from the bottom to the top of the drain *** addition,natural convection intensity increases with increase in height of the heat pipes in the *** between heat pipes has no obvious effect on the natural convection heat transfer *** study will contribute to the design of passive heat removal systems for advanced nuclear reactors.

关键词： passive characteristics pipes

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Development and evaluation of an electropositive wellbore stabilizer with flexible adaptability for drilling strongly hydratable shales

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Petroleum Science 2015年第3期12卷 458-469页

作者： Wei-An Huang zheng-Song qiu Ming-Lei Cui Xin Zhao Jun-Yi Liu Wei-Ji Wang School of Petroleum Engineering China University of Petroleum School of Mechanical and Chemical Engineering The University of Western Australia Shandong Shengli Vocational College

In order to overcome serious instability prob- lems in hydratable shale formations, a novel electropositive wellbore stabilizer （EPWS） was prepared by a new approach. It has good colloidal stability, particle size dis- tribution, compatibility, sealing property, and flexible adaptability. A variety of methods including measurements of particle size, Zeta potential, colloidal stability, contact angle, shale stability index, shale dispersion, shale swelling, and plugging experiments were adopted to characterize the EPWS and evaluate its anti-sloughing capacity and flexible adaptability. Results show that the EPWS has advantages over the conventional wellbore stabilizer （ZX-3） in particle size distribution, colloidal stability, inhibition, compatibil- ity, and flexible adaptability. The EPWS with an average particle size of 507 nm and an average Zeta potential of 54 mV could be stable for 147 days and be compatible with salt tolerant or positive charged additives, and it also exhibited preferable anti-sloughing performance to hydrat- able shales at 77, 100, and 120 ~C, and better compatibility with sodium bentonite than ZX-3 and KC1. The EPWS can plug micro-fractures and pores by forming a tight external mud cake and an internal sealing belt to retard pressure transmission and prevent filtrate invasion, enhancing hydrophobicity of shale surfaces by adsorption to inhibithydration. The EPWS with flexible adaptability to tem- perature for inhibition and sealing capacity is available for long open-hole sections during drilling.

关键词： Shale Wellbore stabilizer Colloidalstability Plugging Hydrophobic modification

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Engineering inorganic lead halide perovskite deposition toward solar cells with efficiency approaching 20%

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Aggregate 2021年第1期2卷 66-83页

作者： Fa-zheng qiu Ming-Hua Li Jun-Jie Qi Yan Jiang Jin-Song Hu Beijing National Laboratory for Molecular Sciences(BNLMS) CAS Key Laboratory of Molecular Nanostructure and NanotechnologyInstitute of ChemistryChinese Academy of SciencesBeijingChina State Key Laboratory for Advanced Metals and Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijingChina Energy Materials and Optoelectronics Unit Songshan Lake Materials LaboratoryDongguanGuangdongChina School of Chemical Sciences University of Chinese Academy of SciencesBeijingChina

Inorganic perovskite materials have gained tremendous attention due to their superior chemical and thermal stability than organic-inorganic hybrid *** the past years,substantial research efforts have been devoted to developing uniform and pin-hole free inorganic perovskite films with high electronic *** a result,power conversion efficiency of inorganic perovskite solar cells(PSCs)has boosted to over 19%,which presents a promising potential for technology ***,we give a comprehensive review of the recent progress on state-of-the-art inorganic cesium lead halide based PSCs,particularly on the perovskite deposition *** show a clear roadmap to fabricate high electronic quality inorganic perovskite films by tuning the precursor crystallization kinetics,performing post-deposition treatments,and passivating surface/interface *** perovskite films prepared by these approaches exhibit not only high crystallinity,favorable morphology,and low trap densities but also improved phase *** advanced deposition approaches lay the foundation for further improving the performance and long-term operational stability of inorganic perovskite photovoltaics.

关键词： efficiency inorganic perovskite methodology solar cell stability

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