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Inactivated SARS-CoV-2 induces acute respiratory distress syndrome in human A CE2-transgenic mice

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Signal Transduction and Targeted Therapy 2022年第1期7卷 310-321页

作者： zhenfei bi Weiqi Hong Haiying Que Cai He Wenyan Ren Jingyun Yang Tianqi Lu Li Chen Shuaiyao Lu Xiaozhong Peng Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of BiotherapyNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityNo.17Block 3Southern Renmin RoadChengduSichuan 610041PR China National Kunming High-level biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKunmingYunnanChina

The development of animal models for COVID-19 is essential for basic research and drug/vaccine *** reported COVID-19 animal models need to be established under a high biosafety level condition for the utilization of live SARS-CoV-2,which greatly limits its application in routine ***,we gen erate a mouse model of COVID-19 un der a gen eral laboratory condition that captures multiple characteristics of SARS-CoV-2-induced acute respiratory distress syndrome(ARDS)observed in huma ***,human ACE2-tra nsge nic(MCE2)mice were in tratracheally in stilled with the formaldehyde-inactivated SARS-CoV-2,resulting in a rapid weight loss and detrimental changes in lung structure and *** pulmonary pathologic changes were characterized by diffuse alveolar damage with pulmonary consolidation,hemorrhage,necrotic debris,and hyaline membrane *** production of fatal cytokines(IL-β,TNF-α,and IL-6)and the infiltration of activated neutrophils,inflammatory monocyte-macrophages,and T cells in the lung were also determined,suggesting the activation of an adaptive immune *** strategies,such as dexamethasone or passive antibody therapy,could effectively ameliorate the disease progression in this ***,the established mouse model for SARS-CoV-2-induced ARDS in the current study may provide a robust tool for researchers in the standard open laboratory to investigate the pathological mechanisms or develop new therapeutic strategies for COVID-19 and ARDS.

关键词： distress lung routine

Tripterin liposome relieves severe acute respiratory syndrome as a potent COVID-19 treatment

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Signal Transduction and Targeted Therapy 2023年第1期8卷 288-301页

作者： Haiying Que Weiqi Hong Tianxia Lan Hao Zeng Li Chen Dandan Wan zhenfei bi Wenyan Ren Min Luo Jingyun Yang Cai He Ailing Zhong Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer CenterNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityChengduSichuanPeople’s Republic of China

For coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),15–30%of patients are likely to develop COVID-19-related acute respiratory distress syndrome(ARDS).There are still few effective and well-understood therapies *** variants and short-lasting immunity are posing challenges to vaccine efficacy,so finding antiviral and antiinflammatory treatments remains ***,tripterin(TP),a traditional Chinese medicine,was encapsulated into liposome(TP lipo)to investigate its antiviral and antiinflammatory effects in severe *** using two severe COVID-19 models in human ACE2-transgenic(hACE2)mice,an analysis of TP lipo’s effects on pulmonary immune responses was *** pathological alterations and viral burden were reduced by TP lipo *** lipo inhibits SARS-CoV-2 replication and hyperinflammation in infected cells and mice,two crucial events in severe COVID-19 pathophysiology,it is a promising drug candidate to treat SARS-CoV-2-induced ARDS.

关键词： immunity alterations distress

A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome

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Signal Transduction and Targeted Therapy 2021年第2期6卷 223-225页

作者： Weiqi Hong Jingyun Yang zhenfei bi Cai He Hong Lei Wenhai Yu Yun Yang Changfa Fan Shuaiyao Lu Xiaozhong Peng Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer CenterNational Clinical Research Center for GeriatricsWest Chino HospitalSichuan UniversityNo.17Block 3Southern Ren min Rood610041 ChengduSichuanPeople's Republic of China National Kunming High-level biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeYun nonChina Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control102629 BeijingChina State Key Laboratory of Medical Molecular biology Deportment of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina

Dear Editor,The COVID-19 pandemic has covered more than 200 countries and regions around the world since its outbreak in January *** date,the SARS-CoV-2 virus has caused>1.2 million *** mortality rate of COVID-19 is closely concerned with the clinical symptoms of the patients from mild-to-severe ***,in its most severe form,COVID-19 leads to life-threatening pneumonia and acute respiratory distress syndrome(ARDS),which is mostly accom-panied by a hyperactive immune response called"cytokine storm"and has high death rates from 40 to 50%.

关键词： distress acute respiratory

Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2536-2549页

作者： Xuemei He Weiqi Hong Jingyun Yang Hong Lei Tianqi Lu Cai He zhenfei bi Xiangyu Pan Yu Liu Lunzhi Dai Wei Wang Canhua Huang Hongxin Deng Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer CenterNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityChengduSichuanPeople’s Republic of China Experimental Medicine Center the Affiliated Hospital of Southwest Medical UniversityLuzhouSichuanPeople’s Republic of China

Mesenchymal stem cell(MSC)-mediated immunomodulation has been harnessed for the treatment of human diseases,but its underlying mechanism has not been fully *** cells,including apoptotic cells have immunomodulatory *** has been repeatedly reported that the proportion of nonviable MSCs in a MSC therapeutic preparation varied from 5-50%in the ongoing clinical *** is conceivable that the nonviable cells in a MSC therapeutic preparation may play a role in the therapeutic effects of *** found that the MSC therapeutic preparation in the present study had about 5%dead MSCs(DMSCs),characterized by apoptotic ***,1×10^(6) MSCs in the preparation contained about 5×10^(4) *** found that the treatment with even 5×10^(4) DMSCs alone had the equal therapeutic effects as with 1×10^(6) *** protective effect of the dead MSCs alone was confirmed in four mouse models,including concanavalin A(ConA)-and carbon tetrachloride(CCI4)-induced acute liver injury,LPS-induced lung injury and spinal cord *** also found that the infused MSCs died by apoptosis in ***,the therapeutic effect was attributed to the elevated level of phosphatidylserine(PS)upon the injection of MSCs or *** direct administration of PS liposomes(PSLs)mimic apoptotic cell fragments also exerted the protective effects as MSCs and *** Mer tyrosine kinase(MerTK)deficiency or the knockout of chemokine receptor C-C motif chemokine receptor 2(CCR2)reversed these protective effects of MSCs or *** results revealed that DMSCs alone in the therapeutic stem cell preparation or the apoptotic cells induced in vivo may exert the same immunomodulatory property as the'living MSCs preparation"through releasing PS,which was further recognized by MerTK and participated in modulating immune cells.

关键词： therapeutic protective preparation

Antibacterial effects and mechanisms of a Chinese folk medicinepreparation “natural plant antimicrobial solution” against Staphylococcus aureus

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Antibacterial effects and mechanisms of a Chinese folk medic...

Correction: Spontaneous apoptosis of cells in therapeutic stemcell preparation exert immunomodulatory effects throughrelease of phosphatidylserine

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Zongying Liu zhenfei bi Yijie Zhou Danzhou Xiangfei Chao Wu Canquan Mao Southwest Jiaotong University

Staphylococcus aureus antibacterialm echanismStaphylococcus aureus remains the leading cause of bacterial infections worldwide rangingfrom minor skin and soft tissue infections to more severe conditions such as bacteraemiaand infective *** the occurrence and discovery of more antibiotic-resistantstrains such as MRSA,MSSA and VRSA,theneed of new and efficient antimicrobial drugsare greatly *** folk medicine may be an alternative and promising candidatefor novel antibacterial drug ***,we investigated the antibacterial effects and primary mechanisms of"natural plantantimicrobial solution"(herein abbreviated to PAMS)prepared by the Institute of Yunnanfolk medicine Co.,Ltd.&Yunnan Puer Danzhou Pharmaceutical Co.,*** *** aureus could be completely killed after treatment with100%concentration of PAMS at minimum of *** 36%concertration of PAMS,Staphylococcusaureus was completely *** order to investigate the antibacterialmechanism of PAMS,the total soluble saccharide concentration and relative electric conductivityof the culture treated with low-dose PAMS were *** was showed thatboth of the total soluble saccharide concentration and relative electric conductivity displayeda tendency of initiatory decrease and later subsequent ***,by employingscanning electron microscope,we could easily find ruptured cell membranes onStaphylococcus aureus and the integrity of the cell membrance was ***’s appropriateto infer that constituents of PAMS might be transferred into the cells or adhered tothe surface of Staphylococcus aureus,which influenced its normal physiological functionand damage and rupture of cell membranes with the release of cellular contents and finallyleading to the death of Staphylococcus aureus.

关键词： natural plant antimicrobial solution PAMS

来源： cnki会议评论

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Signal Transduction and Targeted Therapy 2022年第2期7卷 595-596页

作者： Xuemei He Weiqi Hong Jingyun Yang Hong Lei Tianqi Lu Cai He zhenfei bi Xiangyu Pan Yu Liu Lunzhi Dai Wei Wang Canhua Huang Hongxin Deng Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer Center National Clinical Research Center for Geriatrics West China Hospital Sichuan University Chengdu Sichuan People’s Republic of China Experimental Medicine Center the Affiliated Hospital of Southwest Medical University Luzhou Sichuan People’s Republic of China

After online publication of the article1,the authors noticed one inadvertent mistake in Fig.5a that needs to be *** detail,the pathological picture of PBS group in Fig.5a is inadvertently duplicated as the image of PBS group in Fig.7b in the main *** duplication is a result of errors in figure assembly,and the correct Fig.5 is provided as *** key findings of the article are not affected by these corrections.

关键词： modulator release preparation

Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells

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Cellular & Molecular Immunology 2022年第5期19卷 577-587页

作者： Weiqi Hong Jingyun Yang Jun Zou zhenfei bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei Laboratory of Aging Research and Cancer Drug Targeting State Key Laboratory of Biotherapy and Cancer CenterNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityNo.17Block 3Southern Renmin RoadChengduSichuan610041China National Kunming High-level biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeYunnanChina Westvac biopharm Co. Ltd.No.618Fenghuang RoadShuangliu DistrictChengduSichuanChina Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control102629BeijingChina State Key Laboratory of Medical Molecular biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina

Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host *** to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse *** histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino *** acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S ***,histones enhance cell-cell ***,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse *** findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19.

关键词： COVID-19 SARS-CoV-2 neutrophil extracellular traps histones sialic acid

硼银共掺杂二氧化钛材料的制备及光催化性能的研究

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化学世界 2020年第3期61卷 176-182页

作者：陈适毕颖洁陈敏卉王伟陈威杨振飞罗洁中南林业科技大学材料科学与工程学院湖南长沙410004

以钛酸四丁酯为钛源,硝酸银和硼酸为掺杂剂,采用溶胶-凝胶法制备硼银共掺杂二氧化钛催化剂,采用紫外可见光漫反射光谱(UV-Vis DRS)、扫描电镜(SEM)、能谱仪(EDS)、X射线衍射光谱(XRD)对样品进行表征。以400W金卤灯为光源降解20mg/L甲基... 详细信息

以钛酸四丁酯为钛源,硝酸银和硼酸为掺杂剂,采用溶胶-凝胶法制备硼银共掺杂二氧化钛催化剂,采用紫外可见光漫反射光谱(UV-Vis DRS)、扫描电镜(SEM)、能谱仪(EDS)、X射线衍射光谱(XRD)对样品进行表征。以400W金卤灯为光源降解20mg/L甲基橙溶液,光照30min分别探究Ag摩尔分数、B摩尔分数、煅烧温度、煅烧时间等制备条件对光催化剂性能的影响。结果表明,银硼共掺杂二氧化钛是结构不规则、大小不均匀的块状颗粒,硼、银离子成功掺入TiO2中。硼银元素的掺入使TiO2的光谱响应范围向可见光方向发生了明显红移。当煅烧温度为400℃,煅烧时间为2h,Ag摩尔分数为1.2%,B摩尔分数为6mol%时,Ag-B-TiO2降解效果最为显著,降解率为89.47%。

关键词：银硼共掺杂二氧化钛光催化溶胶-凝胶法

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某钨矿山多金属硫化矿综合回收试验研究

有色金属（选矿部分） 2017年第5期 35-41页

作者：李平黄云松沈新春李振飞赣州有色冶金研究所江西赣州341000

针对某钨矿山多金属硫化矿的矿石性质特点,开展了优先浮钼—铜铋混合浮选工艺、优先浮钼—浮铜—浮铋工艺和钼铜混合浮选—浮铋—浮锌的工艺流程对比试验,结果表明:采用钼铜混合浮选—浮铋—浮锌的全流程工艺,可获得含钼53.50%、回收率... 详细信息

针对某钨矿山多金属硫化矿的矿石性质特点,开展了优先浮钼—铜铋混合浮选工艺、优先浮钼—浮铜—浮铋工艺和钼铜混合浮选—浮铋—浮锌的工艺流程对比试验,结果表明:采用钼铜混合浮选—浮铋—浮锌的全流程工艺,可获得含钼53.50%、回收率为92.72%的钼精矿,含铋11.30%、回收率为58.71%的铋精矿,含铜22.89%、回收率为87.62%的铜精矿,含锌55.28%、回收率为73.22%的锌精矿;而且铋精矿中含银9 000 g/t、含铅58.23%,回收率分别为66.89%、77.40%;同时浮选尾矿进一步回收可获得含钨38.52%、回收率为79.57%的钨精矿,实现了钼、铋、铜、锌、铅和钨的综合回收。

关键词：多金属硫化矿钼铜混合浮选综合回收