检索结果-南通市图书馆

Composition,function,and timing:exploring the early‑life gut microbiota in piglets for probiotic interventions

Journal of Animal Science and Biotechnology 2024年第2期15卷 659-678页

作者： Jianping Quan Cineng Xu Donglin Ruan Yong Ye Yibin Qiu Jie wu Shenping Zhou Menghao Luan Xiang Zhao Yue Chen Danyang Lin Ying Sun Jifei Yang Enqin Zheng Gengyuan Cai zhenfang wu Jie Yang College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhouGuangdongPeople’s Republic of China Guangdong Provincial Key Laboratory of Agro‑Animal Genomics and Molecular Breeding South China Agricultural UniversityGuangzhouGuangdongChina Yunfu Subcenter of Guangdong Laboratory for Lingnan Modern Agriculture YunfuGuangdongChina National Engineering Research Center for Breeding Swine Industry Wens Foodstuff Group Co.Ltd.YunfuGuangdongPeople’s Republic of China

Background The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous ***,the optimal timeframe for introducing probiotics to achieve this outcome remains *** In the context of this investigation,we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time ***,we gathered vaginal and fecal samples from 23 *** 16S rRNA gene and metagenomic sequencing methodologies,we conducted a comprehensive analysis of the fluctuation patterns in microbial composition,functional capacity,interaction networks,and colonization resistance within the gut microbiota of *** the piglets progressed in age,discernible modifications in intestinal microbial diversity,composition,and function were observed.A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal *** D21,the microbial interaction network displayed a more concise and efficient configuration,accompanied by enhanced colonization resistance relative to the other two time ***,we identified three strains of Ruminococcus *** D10 as potential candidates for improving piglets’weight gain during the weaning *** The findings of this study propose that D10 represents the most opportune juncture for the introduction of external probiotic interventions during the early stages of piglet *** investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forthcoming probiotic interventions.

关键词： Colonization resistance Intestinal microbiota Piglet Weaning Weight gain

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy

引用

Science China(Life Sciences) 2024年第8期67卷 1676-1696页

作者： Xupeng Zang Shengchen Gu Wenjing Wang Junsong Shi Jianyu Gan Qun Hu Chen Zhou Yue Ding Yanjuan He Lei Jiang Ting Gu Zheng Xu Sixiu Huang Huaqiang Yang Fanming Meng Zicong Li Gengyuan Cai Linjun Hong zhenfang wu State Key Laboratory of Swine and Poultry Breeding Industry National Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding South China Agricultural UniversityGuangzhou 510642China Yunfu Subcenter of Guangdong Laboratory for Lingnan Modern Agriculture Yunfu 527300China Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China Key Laboratory of South China Modern Biological Seed Industry Ministry of Agriculture and Rural AffairsGuangzhou 510520China

Dynamic crosstalk between the embryo and mother is crucial during ***,we comprehensively profile the single-cell transcriptome of pig peri-implantation embryos and corresponding maternal endometrium,identifying 4 different lineages in embryos and 13 cell types in the ***-specific gene expression characterizes 4 distinct trophectoderm subpopulations,showing development from undifferentiated trophectoderm to polar and mural *** expression of genes in different types of endometrial cells illustrates their molecular response to embryos during ***,we developed a novel tool,ExtraCellTalk,generating an overall dynamic map of maternal-foetal crosstalk using uterine luminal proteins as *** cross-species comparisons,we identified a conserved RBP4/STRA6 pathway in which embryonic-derived RBP4 could target the STRA6 receptor on stromal cells to regulate the interaction with other endometrial *** results provide insight into the maternal-foetal crosstalk during embryo implantation and represent a valuable resource for further studies to improve embryo implantation.

关键词： endometrial remodeling ExtraCellTalk implantation maternal-foetal crosstalk trophoblast

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于目标检测仔猪乳头计数及乳房形态评估方法

引用

农业工程学报 2024年第3期40卷 156-164页

作者：李熙雅尹令黄文杰吴珍芳蔡更元田绪红华南农业大学数学与信息学院广州510642 华南农业大学动物科学学院广州510642 国家生猪种业工程技术研究中心广州510642 猪禽种业全国重点实验室广州510640

母猪乳头数量是生猪选育中重要参考指标之一,也是生猪繁殖表型数据的组成部分。成年母猪其腹部视频较难获取,且容易受污渍干扰,乳头自动点数实现难度较大,人工计数母猪乳头数工作量大、强度高、效率低、容易产生人为误差。鉴于猪仔从出... 详细信息

母猪乳头数量是生猪选育中重要参考指标之一,也是生猪繁殖表型数据的组成部分。成年母猪其腹部视频较难获取,且容易受污渍干扰,乳头自动点数实现难度较大,人工计数母猪乳头数工作量大、强度高、效率低、容易产生人为误差。鉴于猪仔从出生到成年乳头数量保持一致性,该研究提出了一种基于仔猪腹部视频的深度学习乳头计数及乳房形态评估方法。通过架设在仔猪初生护理平台上的相机拍摄仔猪腹部视频,根据清晰度筛选出细节清晰的帧序列图像集,经过数据预处理再使用改进Pignip-YOLOv5s目标检测网络对仔猪乳头进行自动计数。为提高计数准确率,帧序列图像集的乳头计数使用滑动窗口取众数得到最终计数结果。试验结果表明,改进的Pignip-YOLOv5s平均精度值(mean average precision, mAP)高达0.97,较YOLOv5原模型具备更高的鲁棒性。该研究方案在113段仔猪腹部视频数据集上测试得到仔猪乳头计数方法准确率达90.26%。同时该研究提出仔猪乳房形态评估参数乳头成对数、乳头间距,从而量化仔猪乳头形态表型特征,构建了母猪乳房外在形态指标,可为母猪选育和繁殖工作提供重要的参考依据。

关键词：模型深度学习视频计数 YOLOv5s 乳头计数乳房形态评估

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

猪三维点云体尺自动计算模型Pig Back Transformer

引用

智慧农业(中英文) 2024年第04期6卷 76-90页

作者：王宇啸石源源陈招达吴珍芳蔡更元张素敏尹令国家生猪种业工程技术研究中心华南农业大学数学与信息学院华南农业大学动物科学学院猪禽种业全国重点实验室

[目的/意义]为了提高体尺关键点定位准确率，猪三维点云体尺自动测量方法会采用点云分割，在各个分割后局部点云定位测量关键点，以减少点云之间相互干扰。然而点云分割网络通常需要消耗较大计算资源，且现有测量点定位效果仍有待提升... 详细信息

[目的/意义]为了提高体尺关键点定位准确率，猪三维点云体尺自动测量方法会采用点云分割，在各个分割后局部点云定位测量关键点，以减少点云之间相互干扰。然而点云分割网络通常需要消耗较大计算资源，且现有测量点定位效果仍有待提升空间。本研究旨在通过设计关键点生成网络从猪体点云中提取出各体尺测量所需关键点。在降低显存资源需求的同时提高测量关键点定位效果，提高体尺测量的效率和精度。[方法]针对猪三维表面点云进行体尺测量，提出了一种定位猪体尺关键点的模型Pig Back Transformer。模型分为两个模块，分别设计了两种改进的Transformer自注意力编码器，第一模块为全局关键点模块，首先设计了一种猪背部边缘点提取算法用于获取边缘点，再使用edge encoder编码器以边缘点集合作为输入，edge encoder的edge attention中加入了边缘点和质点的偏移距离信息；第二模块为关键点生成模块，使用了back attention机制的back encoder，其中加入了与质心和第一模块生成的全局关键点的偏移量，并将偏移量与点云注意力通过按位max pooling操作结合，最后通过生成猪的体尺测量关键点和背脊走向点。最后设计了使用关键点和背脊走向点作为输入的体尺算法。[结果和讨论]对比关键点和背脊走向点生成任务上Pig Back Transformer表现最佳，并对比体尺计算结果与人工测量结果，体长相对误差为0.63%，相对PointNet++、Point Transformer V2、Point Cloud Transforme、OctFormer PointTr等模型有较大提升。[结论] Pig Back Transformer能相对准确地生成猪体尺关键点，提高体尺测量数据准确度，并且通过点云特征定位体尺关键点节省了计算资源，为无接触牲畜体尺测量提供了新思路。

关键词： Pig Back Transformer 三维点云体尺自动测量测量关键点定位深度相机自注意力机制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Incombustible solid polymer electrolytes:A critical review and perspective

引用

Journal of Energy Chemistry 2024年第6期93卷 264-281,I0007页

作者： Kai wu Jin Tan zhenfang Liu Chenguang Bao Ao Li Qi Liu Baohua Li College of Materials Science and Engineering Hunan UniversityChangsha 413000HunanChina Tsinghua Shenzhen International Graduate School Tsinghua UniversityShenzhen 055GuangdongChina

Since the advent of the solid-state batteries,employing solid polymer electrolytes(SPEs)to replace routine flammable liquid electrolytes is regarded to be one of the most promising solutions in pursing highenergy-density battery *** with superior thermal stability,good processability,and high mechanical modulus obtain increasing ***,SPE-based batteries are not impenetrable due to their decomposition and combustibility under extreme *** believe incorporating appropriate flame-retardant additives/solvents/fragments into SPEs can intrinsically reduce their flammability to solve the battery safety *** this review,the recent research progress of incombustible SPEs,with special emphasis on flame-retardant structural design,is ***,a brief introduction of flame-retardant mechanism,evaluation index for safety of SPEs,and a detailed overview of the latest advances on diverse-types SPEs in various battery systems are *** deep insight into thermal ru naway process,the free-standing incombustible GPEs,and the ratio nal design of pouch cell structures may be the main directions to motivate revolutionary next-generation for safety batteries.

关键词： Non-flammable electrolyte Solid polymer electrolyte High safety electrolyte Solid state electrolyte Solid state battery

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Improving the accuracy of genomic prediction for meat quality traits using whole genome sequence data in pigs

引用

畜牧与生物技术杂志:英文版 2023年第5期14卷 1880-1894页

作者： Zhanwei Zhuang Jie wu Yibin Qiu Donglin Ruan Rongrong Ding Cineng Xu Shenping Zhou Yuling Zhang Yiyi Liu Fucai Ma Jifei Yang Ying Sun Enqin Zheng Ming Yang Gengyuan Cai Jie Yang zhenfang wu College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding Guangzhou 510642China College of Animal Science and Technology Zhongkai University of Agriculture and EngineeringGuangzhou 510225China Yunfu Subcenter of Guangdong Laboratory for Lingnan Modern Agriculture Yunfu 527400China

Background Pork quality can directly affect customer purchase tendency and meat quality traits have become valu-able in modern pork ***,genetic improvement has been slow due to high phenotyping *** this study,whole genome sequence(WGS)data was used to evaluate the prediction accuracy of genomic best linear unbiased prediction(GBLUP)for meat quality in large-scale crossbred commercial *** We produced WGS data(18,695,907 SNPs and 2,106,902 INDELs exceed quality control)from 1,469 sequenced Duroc×(Landrace×Yorkshire)pigs and developed a reference panel for meat quality including meat color score,marbling score,L*(lightness),a*(redness),and b*(yellowness)of genomic *** prediction accuracy was defined as the Pearson correlation coefficient between adjusted phenotypes and genomic estimated breeding values in the validation *** different marker density panels derived from WGS data,accuracy differed substantially among meat quality traits,varied from 0.08 to *** showed that MultiBLUP outperform GBLUP and yielded accuracy increases ranging from 17.39%to 75%.We optimized the marker density and found medium-and high-density marker panels are beneficial for the estimation of heritability for meat ***,we conducted genotype imputation from 50K chip to WGS level in the same population and found average concord-ance rate to exceed 95%and r^(2)=*** Overall,estimation of heritability for meat quality traits can benefit from the use of WGS *** study showed the superiority of using WGS data to genetically improve pork quality in genomic prediction.

关键词： Genomic prediction Meat quality Pigs Whole genome sequence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tel1 and Rif2 oppositely regulate telomere protection at uncapped telomeres in Saccharomyces cerevisiae

引用

Journal of Genetics and Genomics 2018年第9期45卷 467-476页

作者： Ling-Li Zhang zhenfang wu Jin-Qiu Zhou CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sdences Shanghai 200031 China School of Life Science and Technology Shanghai Tech University Shanghai 201210 China

It has been well documented that Tel1 positively regulates telomere-end resection by promoting Mre11-Rad50-Xrs2（MRX） activity, while Rif2 negatively regulates telomere-end resection by inhibiting MRX activity. At uncapped telomeres, whether Tel1 or Rif2 plays any role remains largely unknown. In this work, we examined the roles of Tel1 and Rif2 at uncapped telomeres in yku70△ and/or cdc13-1 mutant cells cultured at non-permissive temperature. We found that deletion of TEL1 exacerbates the temperature sensitivity of both yku70△ and cdc13-1 cells. Further epistasis analysis indicated that MRX and Tel1 function in the same pathway in telomere protection. Consistently, TEL1 deletion increases accumulation of Exo1-dependent telomeric single-stranded DNA（ssDNA） at uncapped telomeres, which stimulates checkpoint-dependent cell cycle arrest. Moreover, TEL1 deletion in yku70△ cells facilitates Rad51-dependent Y0 recombination. In contrast, RIF2 deletion in yku70△ cells decreases the accumulation of telomeric ssDNA after 8 h of incubation at the non-permissive temperature of 37℃ and suppresses the temperature sensitivity of yku70△ cells, likely due to the increase of Mre11 association at ***, our findings indicate that Tel1 and Rif2 regulate telomere protection at uncapped telomeres via their roles in balancing MRX activity in telomere resection.

关键词： Tel1 RiF2 MRX Uncapped telomeres Telomere protection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SAR-GMTI investigation in hybrid along-and cross-track baseline InSAR

引用

Science in China(Series F) 2009年第8期52卷 1399-1408页

作者： SUO ZhiYong LI zhenfang BAO Zheng wu JianXin National Key Lab of Radar Signal Processing Xidian University Xi'an 710071 China

A joint-pixel clutter suppression method based on slope compensation is proposed in this paper, In order to eliminate the effect of the terrain interferometric phase caused by the cross-track baseline in hybrid baseline InSAR, the local independent identical distribution of the clutter is satisfied by using the slope compensation technique, and thus the clutter can be suppressed successfully by using the orthogonality of the clutter subspace and the noise subspace. This approach utilizes the information contained in the current pixel as well as in its neighbors, showing robustness to the image coregistration errors. Both the simulated data and the real airborne data are used in proving the validity of the presented approach.

关键词： interferometric synthetic aperture radar (InSAR) along-/cross-track baseline ground moving target indication (GMTI) slope compensation clutter suppression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

PIK-75 promotes homology-directed DNA repair

引用

Journal of Genetics and Genomics 2019年第3期46卷 141-144页

作者： Guoling Li Xianwei Zhang Hao Ou Haoqiang Wang Dewu Liu Huaqiang Yang zhenfang wu College of Animal Science South China Agricultural University

Homo!ogy-directed repair(HDR)is one of two major DNA repair pathways to mend the double-strand breaks(DSBs)formed in the genome(Liang et al.,1998;Pardo et al.,2009).Although less efficient compared with another DNA repair pathway,nonhomologous end joining(NHEJ),HDR is a type of precise repair to restore DNA damage and sustain genomic stability(Pardo et al.,2009;Ceccaldi et al.,2016).By contrast,NHEJ usually introduces mutations into the repaired site,thus probably harming the genomic integrity(Lieber et al.,2003).The error-free property enables HDR to be harnessed to correct a faulty mutation for therapeutic purpose in cells or in the body(wu et al.,2013).In add让ion,HDR possesses great potential in the generation of genome-edited animals with precise genetic modifications,such as point mutation,DNA replacement,and DNA insertion in a specific genomic site(Wang et al.,2013).However,the low repair frequency mediated by HDR significantly limits让s application for efficient gene correction or establishment of various genetically modified animal ***,multiple site-specific endonucleases have emerged as highly efficient tools to create targeted DSBs and markedly promote subsequent DNA repair either via HDR or NHEJ(Gaj et al.,2013).Nonetheless,the HDR-mediated modifications following the cleavage of engineering nucleases are still inefficient,usually with an efficiency less than 20%in cultured mammalian cells and embryos(Mali et al..2013;Wang et al.,2013;Yang et al.,2013).

关键词： ogy-directed repair major DNA repair mammalian cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

裂殖酵母Shelterin五元复合物的电镜结构研究

引用

上海交通大学学报（医学版） 2022年第3期42卷 290-297页

作者：鲁彦伽孙虹吴振芳雷鸣上海交通大学医学院附属第九人民医院上海精准医学研究院上海200125

目的·通过电子显微镜(电镜)和负染技术分析裂殖酵母端粒结合蛋白质Shelterin五元复合物(Rap1-Poz1-Tpz1-Ccq1-Pot1)的三维结构。方法·利用大肠埃希菌表达系统在体外重组和表达带有组氨酸-小分子泛素相关修饰物蛋白(histidine-small ub... 详细信息

目的·通过电子显微镜(电镜)和负染技术分析裂殖酵母端粒结合蛋白质Shelterin五元复合物(Rap1-Poz1-Tpz1-Ccq1-Pot1)的三维结构。方法·利用大肠埃希菌表达系统在体外重组和表达带有组氨酸-小分子泛素相关修饰物蛋白(histidine-small ubiquitin related modifier,His-SUMO)和谷胱甘肽巯基转移酶(glutathione S-transferase,GST)纯化标签的目的蛋白质,通过两步亲和层析(镍离子金属螯合亲和层析、谷胱甘肽琼脂糖凝胶亲和层析)以及一步凝胶过滤层析(SuperoseTM610/300 GL)分离纯化目的蛋白质复合物。采用0.75%甲酸双氧铀对蛋白质样品进行染色,用120 kV透射电镜观察,放大倍数设置为92000倍,收集颗粒分散性较好的负染照片。用EMAN2软件和Relion3.0软件,通过单颗粒重构技术研究裂殖酵母Shelterin复合物的三维结构,最后利用UCSF Chimera软件对裂殖酵母Shelterin复合物的重构模型进行分析。结果·经过两步亲和层析以及凝胶过滤层析获得纯度高、组分齐、均一性良好的重组裂殖酵母Shelterin五元复合物的蛋白质样品;利用120 kV透射电镜获得负染电镜照片83张,从中挑选18659个蛋白质复合物颗粒进行三维模型构建,初步解析了该复合物的三维结构;将重构模型与已报道的蛋白质组分的高分辨率结构进行分子对接,确定各组分在复合物中的定位情况,揭示该复合物以二聚体的形式存在。结论·构建了裂殖酵母Shelterin五元复合物(Rap1-Poz1-Tpz1-Ccq1-Pot1)的低分辨率的三维模型。

关键词：端粒端粒末端保护 Shelterin复合物电子显微镜负染

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：