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Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Acta Oceanologica Sinica 2005年第4期24卷 155-161页

作者： zhang zhendong zhang Peijun MO Zhaolan WANG Chunling YU Yang Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.

关键词： Vibrio anguillarum extracellular products protease dot-ELISA indirect ELISA Westem blot

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Inhaled multilevel size-tunable,charge-reversible and mucus-traversing composite microspheres as trojan horse:Enhancing lung deposition and tumor penetration

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Chinese Chemical Letters 2024年第9期35卷 273-278页

作者： Lishan Xiong Xinyuan Li Xiaojie Lu zhendong zhang Yan zhang Wen Wu Chenhui Wang Chongqing Key Laboratory of Natural Product Synthesis and Drug Research School of Pharmaceutical SciencesChongqing UniversityChongqing 401331China Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System Chongqing Engineering Research Center of Pharmaceutical SciencesChongqing Medical and Pharmaceutical CollegeChongqing 404120China

Dry powder inhalation represents a promising approach for the treatment of lung cancer,offering several advantages such as enhanced targeting,improved bioavailability,and reduced ***,traditional dry powder formulations suffer from limitations,notably low pulmonary delivery efficiency and inadequate penetration into tumor tissues,thereby limiting their therapeutic *** response to these challenges,we have developed an innovative trojan horse strategy,harnessing an inhalable nanoparticlein-microsphere system characterized by tunable size,reversible charge,and mucus-penetrating *** inhalable nanoparticle-in-microsphere system exhibit stable structural properties,excellent environmental responsiveness and high *** importantly,the therapeutic effect of MTX@PAMAM@HA@Gel(MPHG)was demonstrated in vitro and in *** system offers improved pulmonary delivery efficiency,enhanced drug retention within tumor tissues,and effective penetration,thus representing a promising strategy in lung cancer treatment.

关键词： Pulmonary delivery Size-tunable nanoparticles Charge-reversible nanoparticles Mucus penetration Lung cancer

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Optimization of the Structural Parameters of a Plastic Centrifugal Pump in the Framework of a Flow Field Analysis

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Fluid Dynamics & Materials Processing 2022年第3期18卷 789-813页

作者： Wenbin Luo Youmin Wang Yuting Yan Yifang Shi zhendong zhang School of Mechanical Engineering Anhui Polytechnic UniversityWuhu241000China Hohai University Nanjing210098China Anhui Institute of Information Technology Wuhu241199China

In order to determine the optimal structural parameters of a plastic centrifugal pump in the framework of an orthogonal-experiment approach,a numerical study of the related flow field has been performed using *** thickness S,outlet angleβ2,inlet angleβ1,wrap angle,and inlet diameter D1 of the splitter blades have been considered as the variable factors,using the shaft power and efficiency of the pump as evaluation *** a parametric analysis,the relative importance of the influence of each structural parameter on each evaluation index has been obtained,leading to the following combinations:β119°,β235°,S 2 mm,wrap angle 154°,and D185 mm(corresponding to the maximum efficiency of 75.48%);β119°,β220°,S 6 mm,wrap angle 158°,and D181 mm(corresponding to the minimum shaft power of 75.48%).Moreover,the grey correlation method has been applied to re-optimize the shaft power and efficiency of the pump,leading to the following optimal combination:β119°,β215°,S 4 mm,D181 mm,and wrap angle 152°(corresponding to the maximum efficiency of 71.81%and minimum shaft power of 2.187 kW).

关键词： Plastic centrifugal pump splitter blade orthogonal experiment grey correlation method optimal combination

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Genome-wide identification and analysis of the DREB genes and their expression profiles under abiotic stresses in Chinese jujube(Ziziphus jujuba Mill.)

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Journal of Forestry Research 2019年第4期30卷 1277-1287页

作者： Heying Zhou Jiping Jia Decang Kong zhendong zhang Shuang Song Yingyue Li Xiaoming Pang Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree BreedingKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China National Foundation for Improved Cultivar of Chinese Jujube Cangzhou 061000HebeiChina

CBF/DREB proteins play a critical role in abiotic stress-mediated gene expression and represent attractive regulons for plant breeding ***,no study has been conducted for CBF/DREB protein-related genes in jujube(Ziziphus jujuba Mill.).In this study,twenty-five ZjDREB genes were identified and annotated from the jujube(***‘Dongzao’)*** analysis,including gene classification,annotation,phylogenetic evaluation,conserved motif determination and expression profiling were performed on all *** analysis showed that ZjDREB proteins were divided into five subgroups(A1–A5),but lacking a subgroup A6 corresponding to *** ZjDREB genes were distributed in nine of twelve chromosomes in the ***,the expression patterns of the DREB genes under different abiotic stresses were investigated using q *** ZjDREB genes were down-regulated under low temperature,in contrast six ZjDREB genes(01,03,05,11,23 and 24)were *** drought,salinity and high temperature conditions,expression of ZjDREB03,09,10,14,15,17 and 20 genes were induced and showed similar expression patterns,suggesting that various stress conditions share common elements in the signaling *** results suggest that the family of DREB genes play an important role in abiotic stresses in jujube,and provide a foundation for further functional studies of this important class of transcriptional regulators.

关键词： Abiotic stress Chinese jujube DREB Expression patterns Phylogenetic analysis

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Evaluating the flavor and divergent bacterial communities in corn-based zha-chili

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Food Bioscience 2022年第2期46卷 403-412页

作者： Zhuang Guo Yurong Wang Fanshu Xiang Yun Dong Qiangchuan Hou zhendong zhang Hubei Provincial Engineering and Technology Research Center for Food Ingredients Hubei University of Arts and ScienceXiangyangHubei provincePR China Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory Hubei University of Arts and ScienceXiangyangHubei provincePR China

Zha-chili is a naturally fermented traditional food from central southern ***-based zha-chili is one of the most popular varieties with a special *** investigate its distinct microbiome,corn-based zha-chili samples were collected and investigated using both high-throughput sequencing and culture-dependent ***,E-nose and E-tongue sensors were used to evaluate the overall sensory *** found that the bacterial communities present in zha-chili samples from the two regions were significantly different(P<0.05).Companilactobacillus,Lactiplantibacillus,and Corynebacterium were found to be the dominant genera common to samples from both *** these,lactic acid bacteria were the most *** genera were Ligilactobacillus,Lactobacillus,and Levilactobacillus(Huaihua)and Pantoea,Lactiplantibacillus,and Weissella(Songtao).Using culture-dependent methods,the most dominant genus Companilactobacillus was found to comprise Companilactobacillus alimentarius and Companilactobacillus *** sensory profile was also *** indicated that zha-chili from the two regions could be discriminated by sourness and aftertaste-B(E-taste),and W1C,W3C,W5C,W1S,W2S,W5S,and W2W(E-nose).Correlation analysis suggested that the dominant genera might not affect the aroma quality of corn-based zha-chili,whereas they were related to taste *** and Ligilactobacillus were positively correlated with the characteristic taste indices and negatively correlated with the off-flavor *** dominant genus,Kocuria was positively correlated with umami and richness(P<0.05).Our results could provide a basis for supporting zha-chili production and help the isolation of appropriate lactic acid bacteria from corn-based zha-chili.

关键词： Corn-based zha-chili Bacterial community Dominant taxa Sensory quality Correlation analysis Culture-dependent method

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A Lamellar Ceria Structure with Encapsulated Platinum Nanoparticles

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Nano Research 2008年第6期1卷 474-482页

作者： Angelo C.Mak Changlin Yu Jimmy C.Yu zhendong zhang Chunman Ho Department of Chemistry The Center of Novel Functional Moleculesand Environmental Science ProgrammeThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina

A novel lamellar feather-like CeO_(2) structure has been fabricated by using a triblock copolymer as the structure-directing *** material was characterized in detail by X-ray diffraction,scanning electron microscopy,transmission electron microscopy,X-ray photoelectron spectroscopy,and BET surface area *** with conventional spherical shaped ceria prepared by ammonia gelation,the ceria feathers have superior ability to support nanosized platinum particles due to their special ***“skeletons”of ceria feathers can serve as an ideal host matrix to anchor the platinum ***,the inter-crossing pattern of the“skeletons”also acts as a partition to separate platinum particles,allowing the Pt nanoparticles(average diameter~6 nm)to be highly dispersed in the *** Pt/feather-like CeO_(2) catalyst exhibits high activity in the water gas shift reaction.

关键词： Ceria nanostructure platinum nanoparticles surfactant-controlled synthesis water gas shift reaction

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Bone density, microarchitecture and stiffness in Caucasian and Caribbean Hispanic postmenopausal American women

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Bone Research 2014年第3期2卷 194-202页

作者： Bin Zhou Ji Wang Emily M Stein zhendong zhang Kyle K Nishiyama Chiyuan A zhang Thomas L Nickolas Elizabeth Shane X Edward Guo Bone Bioengineering Laboratory Department of Biomedical Engineering Columbia University Division of Endocrinology Department of Medicine Columbia University Department of Orthopedic Surgery First Affiliated Hospital School of Medicine Shihezi University

Hispanic Americans of Caribbean origin are a fast-growing subset of the US population, but there are no studies on bone density, microstructure and biomechanical integrity in this minority group. In this study, we aimed to compare Caucasian and Caribbean Hispanic postmenopausal American women with respect to these characteristics. Thirty-three Caribbean Hispanics were age-matched to thirty-three Caucasian postmenopausal women. At the lumbar spine, the Hispanic women had significantly lower areal bone mineral density （aBMD）. At the radius by high-resolution peripheral quantitative computed tomography （HR-pQCT）, there were minimal differences between Hispanic and Caucasian women. At the tibia, Hispanic women had lower trabecular volumetric bone density and trabecular number, and higher trabecular separation. Individual trabecula segmentation （ITS） analyses indicated that at the tibia, Hispanic women not only had significantly lower bone volume fraction, but also had significantly lower rod bone volume fraction, plate trabecular number, rod trabecular number and lower plate-plate, plate-rod and rod-rod junction densities compared to Caucasian women. The differences in bone quantity and quality contributed to lower whole bone stiffness at the radius, and both whole bone and trabecular bone stiffness at the tibia in Hispanic women. In conclusion, Hispanic women had poorer bone mechanical and microarchitectural properties than Caucasian women, especially at the load-bearing distal tibia.

关键词： bone DXA Bone density microarchitecture and stiffness in Caucasian and Caribbean Hispanic postmenopausal American women

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二自由度平面机械手的 T-S 模型模糊自适应控制

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清华大学学报（自然科学版） 1998年第3期38卷 54-57页

作者：张振东孙增圻清华大学计算机科学与技术系河北工业大学

为解决二自由度平面机械手的控制问题，提出基于Ｔ－Ｓ模糊模型和并行分配补偿（ｐａｒａｌｅｌｄｉｓｔｒｉｂｕｔｅｄｃｏｍｐｅｎ－ｓａｔｉｏｎ）法的模糊模型参考自适应控制方法。并提出采用初始条件补偿回路，消除参考模型和实际... 详细信息

为解决二自由度平面机械手的控制问题，提出基于Ｔ－Ｓ模糊模型和并行分配补偿（ｐａｒａｌｅｌｄｉｓｔｒｉｂｕｔｅｄｃｏｍｐｅｎ－ｓａｔｉｏｎ）法的模糊模型参考自适应控制方法。并提出采用初始条件补偿回路，消除参考模型和实际系统之间由于初始条件不同所引起的误差和实际系统参数变化引起的部分不能被自适应调节律消除的误差。根据李雅普诺夫稳定性理论，求得反馈控制律、稳定条件和调节律（自适应律）。最后，对连续系统情况进行了仿真验证。所提方法可用于解决一类非线性系统的控制问题。

关键词：自适应控制 T-S模型模糊控制参考模型机械手

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EXPERIMENTAL STUDY OF FRETTING WEAR OF TITANIUM ALLOY BEFORE AND AFTER LASER BEAM QUENCHING

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Chinese Journal of Aeronautics 1997年第3期10卷 48-53页

作者： Dai zhendong, Tang Yaxing, zhang Hong, Wang Min (Department of Mechanical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China) Yang Shenrong, zhang Xueshou (Laboratory of Solid Lubrication, Lanzhou Institu Department of Mechanical Engineering Nanjing Univ. Aero. and Astronaut. Nanjing 210016 China Laboratory of Solid Lubrication Lanzhou Inst. of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 China

The effects of amplitudes, normal loads and laser beam quenching on the fretting wear of titanium alloy (TC11) were experimentally investigated on SRV fretting wear test machine in air, at room temperature and without lubrication conditions. The purpose of this study is to learn the rules of fretting wear in a disk blades dovetail joint of an aircraft turbine so the test parameters are determined based on the relative movement and load in the joint. The wear depths are measured by a profilometer, the worn areas are observed and measured by an optical microscopy, and the microtopography of the worn scar is studied by scanning electron microscopy (SEM) .The tests and observations state clearly that fretting wear rate (FWR) is heavily influenced by sliding amplitude(SA) and load. In this experiment, if SA is greater than 60 μm at Hertz contact stress 105 MPa, the FWR is much higher, and the SEM makes it known that the wear mechanism is the combination of adhesive and contact fatigue in the above test conditions. In contrast, if SA smaller, the FWR lower too, and the SEM suggests that the major wear mechanism is contact fatigue. The experiments also reveal that the laser beam quenching greatly improve the fretting wear resistance of titanium alloy, especially at heavy load and large amplitude.

关键词： fretting wear laser beams quenching (cooling) titanium alloys

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The Origin of PRRSV and Advances in the Evolution and Pathogenicity of PRRSV 1

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Animal Husbandry and Feed Science 2022年第1期14卷 27-31页

作者： Xu Xiaozhou zhang zhendong Xing Jun Lian Xue Shi Hongjing zhang Shoudong Jiangsu Vocational College of Agriculture and Forestry Zhenjiang 212400China College of Biotechnology Jiangsu University of Science and TechnologyZhenjiang 212018China Changjia(Shanghai)Agricultural Science and Technology Co. Ltd.Shanghai 201600China

Porcine reproductive and respiratory syndrome virus(PRRSV) has been mutating and evolving constantly since its emergence in the1980s, which has brought inestimable economic losses to the global swine industry. The virus has two genotypes, of which genotype 1 PRRSV(PRRSV 1) first broke out in Germany and mainly prevailed in Europe, which can be clustered into four subtypes based on the ORF5 sequence. Al-though few cases of PRRSV 1 have been reported in China, the prevention and control of PRRSV should not be ignored. The origin of PRRSV, ge-netic evolution and pathogenicity of PRRSV 1 were retrospectively analyzed, in order to provide valuable evidences for molecular epidemiology and immune prevention and control of PRRSV 1.

关键词： Porcine reproductive and respiratory syndrome virus(PRRSV) PRRSV genotype 1 Origin Variation Evolution Pathogenicity

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