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A novel pathogen Fusarium cuneirostrum causing common bean(Phaseolus vulgaris)root rot in China

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Journal of Integrative Agriculture 2024年第1期23卷 166-176页

作者： Dong Deng Wenqi Wu Canxing Duan Suli Sun zhendong zhu Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Several fungal pathogens cause root rot of common bean,among which Fusarium *** the most common pathogens causing Fusarium root rot(FRR)*** has been becoming an increasingly severe disease of common bean in China,but the species of Fusarium *** remained ***,this study was performed to identify the pathogen causing common bean root rot in Liangcheng County,Inner Mongolia,*** Fusarium-like isolates were obtained after pathogen isolation and *** pathogenicity test indicated that eight isolates caused severe disease symptoms on common bean,while 11 other isolates were not *** eight pathogenic isolates,FCL1–FCL8,were identified as Fusarium cuneirostrum by morphological characterization and phylogenetic analysis using partial sequences of EF-1α,ITS,28S,and IGS *** range test showed that the representative *** isolate FCL3 was also pathogenic to mung bean,while not pathogenic to adzuki bean,chickpea,cowpea,faba bean,pea,and ***,50 common bean and 50 mung bean cultivars were screened for resistance to FRR,and seven highly resistant or resistant cultivars of common bean were identified,while no resistant cultivars of mung bean were *** study revealed that *** was one of common bean FRR pathogens in Inner Mongolia and it could induce mung bean root rot as *** our knowledge,this is the first report of *** causing FRR of common bean in China.

关键词： Fusarium cuneirostrum fusarium root rot pathogenicity molecular phylogenetic analysis Phaseolus vulgaris

Fano resonance boosted cascaded optical field enhancement in a plasmonic nanoparticle-in-cavity nanoantenna array and its SERS application

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Light(Science & Applications) 2015年第1期4卷 330-336页

作者： zhendong zhu Benfeng Bai Oubo You Qunqing Li Shoushan Fan State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua UniversityBeijing 100084China TsinghuaFoxconn Nanotechnology Research Center Department of PhysicsTsinghua UniversityBeijing 100084China

Cascaded optical field enhancement(CFE)can be realized in some specially designed multiscale plasmonic nanostructures,in which the generation of extremely strong fields at nanoscale volume is crucial for many applications,for example,surface-enhanced Raman spectroscopy(SERS).In this paper,we propose a strategy for realizing a high-quality plasmonic nanoparticle-in-cavity(PIC)nanoantenna array,in which strong coupling between a nanoparticle(NP)dark mode with a high-order nanocavity bright mode can produce strong Fano resonance at the target *** Fano resonance can effectively boost the CFE in a PIC.A cost-effective and reliable nanofabrication method is developed using room temperature nanoimprinting lithography to manufacture high-quality PIC *** technique guarantees the generation of only one gold NP at the bottom of each nanocavity,which is crucial for the generation of the expected *** demonstrate the performance and application of the PIC array,the PIC array is employed as an active SERS substrate for detecting 4-aminothiophenol *** experimental SERS enhancement factor of 2×10^(7) is obtained,which verifies the field enhancement and the potential of this device.

关键词： cascaded optical field enhancement Fano resonance localized surface plasmon plasmonic nanoantenna surfaceenhanced Raman spectroscopy

Two major er1 alleles confer powdery mildew resistance in three pea cultivars bred in Yunnan Province, China

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The Crop Journal 2016年第5期4卷 353-359页

作者： Suli Sun Yuhua He Cheng Dai Canxing Duan zhendong zhu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Yunnan Academy of Agricultural Sciences

Powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical means of controlling this disease. The objectives of this study were to screen Chinese elite pea cultivars for resistance to E. pisi and to identify the responsible gene at the er1 locus. Among the 37 pea cultivars tested, three(Yunwan 8, Yunwan 21, and Yunwan 23) were immune to E. pisi infection in phenotypic evaluations. The full-length cD NA sequences of the er1 candidate gene, PsM LO1, from the three resistant cultivars and control plants were analyzed. Comparison of the cD NA sequences of 10 clones revealed differences among the powdery mildew-resistant cultivars, susceptible controls, and wild-type cultivar Sprinter. The observed resistance in Yunwan 8 plants resulted from a point mutation(C → G) at position 680 of PsM LO1 that introduced a stop codon, leading to premature termination of protein synthesis. The responsible resistance allele was identified as er1–1. Powdery mildew resistance in Yunwan 21 and Yunwan 23 plants was caused by identical insertions or deletions in PsM LO1. Three distinct PsM LO1 transcripts were observed in Yunwan 21 and Yunwan 23 plants. These transcripts were characterized by a129-bp deletion and 155- and 220-bp insertions, respectively. The responsible resistance allele was identified as er1–2. We have characterized two important er1 alleles in three E. pisi-resistant pea cultivars bred in Yunnan Province, China. These cultivars represent important genetic resources for the breeding of powdery mildew-resistant pea cultivars.

关键词： Erysiphe pisi er1 Pea Powdery mildew Yunnan Province

QTL and candidate genes associated with common bacterial blight resistance in the common bean cultivar Longyundou 5 from China

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The Crop Journal 2016年第5期4卷 344-352页

作者： Jifeng zhu Jing Wu Lanfen Wang Matthew W.Blair zhendong zhu Shumin Wang Institute of Crop Science Chinese Academy of Agricultural Sciences Department of Agriculture & Environment Tennessee State University

Common bacterial blight(CBB), caused by Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans(Xff), is a worldwide disease of common bean(Phaseolus vulgaris L.).Longyundou 5, a Chinese cultivar in the Mesoamerican gene pool of common bean, displays resistance to the Xff strain XSC3-1. To identify the genetic mechanisms behind this resistance,we crossed Long 5 with a susceptible genotype to develop a mapping population of F2 *** resistance to CBB was identified at 14 and 21 days after inoculation with Xff strain XSC3-1.A major QTL at 14 and 21 days after inoculation was mapped on chromosome Pv10 with LOD scores of 6.41 and 5.35, respectively. This locus was associated with SAP6, a previouslyidentified and much-used dominant marker, but in a 4.2 cM interval between new codominant markers BMp10s174 and BMp10s244. Ten candidate genes were found between markers BMp10s174 and BMp10s244 on chromosome Pv10 and could encode defense response proteins responding to CBB pathogens. Four pairs each of epistatic QTL for CBB resistance were detected at 14 and 21 days after inoculation. Phenotypic variation explained by the epistatic QTL ranged from 7.19% to 12.15% and 7.72% to 8.80% at 14 and 21 days after inoculation, respectively. These results confirmed the importance of epistasis in CBB resistance in common bean. The adjacent markers found may be more efficient for marker assisted selection in common bean breeding for CBB resistance owing to their closer linkage to the target QTL.

关键词： Common bean(Phaseoius vulgaris L.) Common bacterial blight Quantitative trait locus Days after inoculation

SFLA算法下综合能源微电网分布式电能鲁棒优化

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电子设计工程 2025年第4期33卷 17-20,24页

作者：曾绘宁朱振东曾权赋三峡大学电气与新能源学院湖北宜昌443002

针对综合能源微电网受可再生能源波动性和不可预测性的影响,导致电能供应波动性较大的问题,采用SFLA算法,优化综合能源微电网分布式电能的鲁棒性。根据综合能源微电网拓扑结构,以最小化日前电能运行成本为目标,构建包括分布式电源成本... 详细信息

针对综合能源微电网受可再生能源波动性和不可预测性的影响,导致电能供应波动性较大的问题,采用SFLA算法,优化综合能源微电网分布式电能的鲁棒性。根据综合能源微电网拓扑结构,以最小化日前电能运行成本为目标,构建包括分布式电源成本、储能系统储能成本、用电负荷补贴成本、配电网购电成本的目标函数。通过对目标函数对偶处理,得到鲁棒优化模型。使用蛙跳算法(Shuffled Frog-Leaping Algorithm,SFLA)求解,通过计算适应度值,更新组内最差个体,获取当前全局最优解。通过算例得出,在14:00时刻,光伏功率达到极大值为60 kW,07:00时刻,储能功率达到极小值-3 kW,研究方法的微电网分布式电能优化结果与理想情况具有高度一致性。

关键词： SFLA算法综合能源微电网分布式电能鲁棒优化对偶处理

Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1

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The Crop Journal 2015年第6期3卷 489-499页

作者： Suli Sun Zhongyi Wang Haining Fu Canxing Duan Xiaoming Wang zhendong zhu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences

Powdery mildew, caused by Erysiphe pisi D.C., is a major constraint to pea production worldwide. The pea cultivar Xucai 1 has shown high resistance to E. pisi under greenhouse and field conditions. The objectives of this study were to identify and characterize genes conferring resistance to powdery mildew in Xucai 1. Three crosses, Qizhen 76 × Xucai 1,Bawan 6 × Xucai 1, and Xucai 1 × Bawan 6, were made to generate populations for genetic analysis. The resistance to E. pisi and segregation ratios in the F_1, F_2, and F_(2:3)populations suggested a single recessive gene conferring the resistance of Xucai 1. Bulked segregant analysis was used to map the resistance gene using two F2 populations. The resistance gene was close to markers AD60 and c5 DNAmet on linkage group VI with genetic distances of9.9 c M and 15.4 c M in the Xucai 1 × Bawan 6 F_2 population and 8.7 c M and 8.1 c M in the Qizhen 76 × Xucai 1 F_2 population, respectively, suggesting that the resistance gene was an er1 allele. This hypothesis was confirmed by comparison of the c DNA sequences of the Ps MLO1 gene between the parents and the Ps MLO1 wild type. Three distinct types of transcripts in Xucai 1, characterized by a 129-bp deletion and 155- and 220-bp insertions,were detected, consistent with the structure of the er1-2 allele. We concluded that resistance in Xucai 1 was conferred by er1-2 and that its linked markers will be useful in pea breeding programs.

关键词： Erysiphe pisi er1-2 Pea Powdery mildew Xucai1

An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo

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Journal of Animal Science and Biotechnology 2016年第2期7卷 158-166页

作者： Jinzhou Qin Haixia Xu Pengfei Zhang Conghui Zhang zhendong zhu Rongfeng Qu Yuwei Qin Wenxian Zeng College of Animal Science and Technology Northwest A&F University

Background： Male germline stem cells（MGSCs） are a subpopulation of germ cells in the testis tissue. MGSCs are capable of differentiation into spermatozoa and thus are perfect targets for genomic manipulation to generate transgenic ***： The present study was to optimize a protocol of production of transgenic mice through transduction of MGSCs in vivo using lentiviral-based vectors. The recombinant lentiviral vectors with either EF-1 or CMV promoter to drive the expression of enhanced green fluorescent protein（e GFP） transgene were injected into seminiferous tubules or inter-tubular space of 7-day-old and 28-day-old mouse testes. At 5 or 6 wk post-surgery, these pre-founders were mated with wild-type C57BL/6J female mice（1.5 to 2.0-month-old）.Results： Sixty-seven percent of F1 generation and 55.56 % of F2 offspring were positive for eG FP transgene under the control of EF-1 promoter via PCR analysis. The transgenic pups were generated in an injection site-and age-independent manner. The expression of transgene was displayed in the progeny derived from lentiviral vector containing CMV promoter to drive transgene, but it was silenced or undetectable in the offspring derived from lentiviral vector with transgene under EF-1 promoter. The methylation level of g DNA in the promoter region of transgene was much higher in the samples derived lentiviral vectors with EF-1 promoter than that with CMV promoter,suggesting e GFP transgene was suppressed by DNA methylation in ***： This research reported here an effective strategy for generation of transgenic mice through transduction of MGSCs in vivo using lentivirus vectors with specific promoters, and the transgenic offspring were obtained in an injection site-and age-independent manner. This protocol could be applied to other animal species, leading to advancement of animal transgenesis in agricultural and biomedical fields.

关键词： In vivo Lentivirus vectors Male germline stem cells Transgenesis

Induced defense responses in rice plants against small brown planthopper infestation

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The Crop Journal 2014年第1期2卷 55-62页

作者： Canxing Duan Jiaojiao Yu Jianyu Bai zhendong zhu Xiaoming Wang Institute of Crop Science Chinese Academy of Agricultural SciencesNational Key Facility for Crop Gene Resources and Genetic Improvement

The small brown planthopper(SBPH), Laodelphax striatellus Fallén(Homoptera: Delphacidae), is a serious pest of rice(Oryza sativa L.) in China. To understand the mechanisms of rice resistance to SBPH, defense response genes and related defense enzymes were examined in resistant and susceptible rice varieties in response to SBPH infestation. The salicylic acid(SA) synthesis-related genes phenylalanine ammonia-lyase(PAL), NPR1, EDS1 and PAD4 were induced rapidly and to a much higher level in the resistant variety Kasalath than in the susceptible cultivar Wuyujing 3 in response to SBPH infestation. The expression level of PAL in the Kasalath rice at 12 h post-infestation(hpi) increased 7.52-fold compared with the un-infested control, and the expression level in Kasalath was 49.63, 87.18, 57.36 and 75.06 times greater than that in Wuyujing 3 at 24, 36, 48 and 72 hpi, respectively. However, the transcriptional levels of the jasmonic acid(JA) synthesis-related genes LOX and AOS2 in resistant Kasalath were significantly lower than in susceptible Wuyujing 3 at 24, 36, 48 and 72 hpi. The activities of the defense enzymes PAL, peroxidase(POD), and polyphenol oxidase(PPO) increased remarkably in Kasalath in response to SBPH infestation, and were closely correlated with the PAL gene transcript level. Our results indicated that the SA signaling pathway was activated in the resistant Kasalath rice variety in response to SBPH infestation and that the gene PAL played a considerable role in the resistance to SBPH.

关键词： Defense enzyme Defense gene Real-time PCR Rice Small brown planthopper

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运输过程中振动对猪精液质量影响的研究进展

黑龙江畜牧兽医 2025年第1期 37-40页

作者：王琦王善鹏刘申奥周开锋曾文先罗亚东朱振东青岛农业大学动物科技学院山东青岛266000 山东省畜牧总站济南250102 西北农林科技大学动物科技学院陕西杨凌712100 杭州东源生物科技有限公司杭州311121

在生产实践中,精液通常通过物流运输方式送达养殖场,但是在运输过程中车辆振动会对猪精子质量造成影响,甚至会损伤精子,进而降低人工授精的成功率。目前尚未建立精液运输过程监测系统,因此如何避免运输过程中振动对精子的损伤是提高养... 详细信息

在生产实践中,精液通常通过物流运输方式送达养殖场,但是在运输过程中车辆振动会对猪精子质量造成影响,甚至会损伤精子,进而降低人工授精的成功率。目前尚未建立精液运输过程监测系统,因此如何避免运输过程中振动对精子的损伤是提高养殖效益的关键因素之一。文章对精液运输过程中振动的产生及其对精液质量的影响研究进行了综述,旨在为提升运输后的精液质量提供参考依据。

关键词：猪精液运输振动精液质量氧化应激